EGFR Monoclonal Antibody (225) | Invitrogen MA5-12880 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Monoclonal Antibody (225)

catalog :

MA5-12880

quantity :

500 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

225

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig 6e	Lisse T, Rieger S. IKKα regulates human keratinocyte migration through surveillance of the redox environment. J Cell Sci. 2017;130:975-988 pubmed publisher
immunocytochemistry; human; fig 2	He Y, Ryu T, Shrestha N, Yuan T, Kim H, Shrestha H, et al. Interaction of EGFR to Î´-catenin leads to Î´-catenin phosphorylation and enhances EGFR signaling. Sci Rep. 2016;6:21207 pubmed publisher
blocking or activating experiments; human	Klotzsche von Ameln A, Prade I, Grosser M, Kettelhake A, Rezaei M, Chavakis T, et al. PHD4 stimulates tumor angiogenesis in osteosarcoma cells via TGF-?. Mol Cancer Res. 2013;11:1337-48 pubmed publisher
immunocytochemistry; human	Terakawa M, Tsunoi Y, Mitsuhashi T. In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse. Int J Nanomedicine. 2012;7:2653-60 pubmed publisher
immunohistochemistry; human	Soonthornthum T, Arias Pulido H, Joste N, Lomo L, Muller C, Rutledge T, et al. Epidermal growth factor receptor as a biomarker for cervical cancer. Ann Oncol. 2011;22:2166-78 pubmed publisher
immunocytochemistry; human	Lu R, Bian F, Zhang X, Qi H, Chuang E, Pflugfelder S, et al. The ?-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells. Int J Biochem Cell Biol. 2011;43:751-9 pubmed publisher
blocking or activating experiments; human western blot; human	Black P, Brown G, Dinney C, Kassouf W, Inamoto T, Arora A, et al. Receptor heterodimerization: a new mechanism for platelet-derived growth factor induced resistance to anti-epidermal growth factor receptor therapy for bladder cancer. J Urol. 2011;185:693-700 pubmed publisher
blocking or activating experiments; human; 10 umol/L	Kajiya M, Shiba H, Komatsuzawa H, Ouhara K, Fujita T, Takeda K, et al. The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics. J Endod. 2010;36:1009-13 pubmed publisher
immunocytochemistry; human; 10 ug/ml	Weigum S, Floriano P, Redding S, Yeh C, Westbrook S, McGuff H, et al. Nano-bio-chip sensor platform for examination of oral exfoliative cytology. Cancer Prev Res (Phila). 2010;3:518-28 pubmed publisher
blocking or activating experiments; human	Kojic N, Chung E, Kho A, Park J, Huang A, So P, et al. An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium. FASEB J. 2010;24:1604-15 pubmed publisher
blocking or activating experiments; human; 10 ug/ml	Leach R, Kilburn B, Petkova A, Romero R, Armant D. Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor. Am J Obstet Gynecol. 2008;198:471.e1-7; discussion 471.e7-8 pubmed publisher
blocking or activating experiments; human	Wolff G, Chiang P, Smith S, Romero R, Armant D. Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells. Biol Reprod. 2007;77:53-60 pubmed
western blot; human	Yasuda H, Hirata S, Inoue K, Mashima H, Ohnishi H, Yoshiba M. Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells. Biochem Biophys Res Commun. 2007;354:154-9 pubmed
blocking or activating experiments; human	Farhan H, Schuster C, Klinger M, Weisz E, Waxenecker G, Schuster M, et al. Inhibition of xenograft tumor growth and down-regulation of ErbB receptors by an antibody directed against Lewis Y antigen. J Pharmacol Exp Ther. 2006;319:1459-66 pubmed
blocking or activating experiments; human; 10 ug/ml	Armant D, Kilburn B, Petkova A, Edwin S, Duniec Dmuchowski Z, Edwards H, et al. Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor. Development. 2006;133:751-9 pubmed
flow cytometry; human; 10 ug/ml	Kim Y, Bhandari R, Cochran J, Kuriyan J, Wittrup K. Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast. Proteins. 2006;62:1026-35 pubmed
blocking or activating experiments; human; 0.5 ug/ml	Wu K, Dupré E, Kim H, Tin U C, Bissonnette R, Lamph W, et al. Receptor-selective retinoids inhibit the growth of normal and malignant breast cells by inducing G1 cell cycle blockade. Breast Cancer Res Treat. 2006;96:147-57 pubmed
immunocytochemistry; human; 1:200	Ward D, Vaughn M, Shiflett S, White P, Pollock A, Hill J, et al. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. J Biol Chem. 2005;280:10548-55 pubmed
	Raimbourg J, Joalland M, Cabart M, de Plater L, Bouquet F, Savina A, et al. Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib. Mol Cancer Ther. 2017;16:1634-1644 pubmed publisher
	Chao G, Cochran J, Wittrup K. Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display. J Mol Biol. 2004;342:539-50 pubmed
	Kansra S, Stoll S, Johnson J, Elder J. Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Mol Biol Cell. 2004;15:4299-309 pubmed
	Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
	Planque S, Taguchi H, Burr G, Bhatia G, Karle S, Zhou Y, et al. Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity. J Biol Chem. 2003;278:20436-43 pubmed
	Planque S, Zhou Y, Nishiyama Y, Sinha M, O Connor McCourt M, Arnett F, et al. Autoantibodies to the epidermal growth factor receptor in systemic sclerosis, lupus, and autoimmune mice. FASEB J. 2003;17:136-43 pubmed
	Jin K, Mao X, Sun Y, Xie L, Jin L, Nishi E, et al. Heparin-binding epidermal growth factor-like growth factor: hypoxia-inducible expression in vitro and stimulation of neurogenesis in vitro and in vivo. J Neurosci. 2002;22:5365-73 pubmed
	Yang J, Kim O, Wu J, Qiu Y. Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking. J Biol Chem. 2002;277:30219-26 pubmed
	Stoll S, Kansra S, Peshick S, Fry D, Leopold W, Wiesen J, et al. Differential utilization and localization of ErbB receptor tyrosine kinases in skin compared to normal and malignant keratinocytes. Neoplasia. 2001;3:339-50 pubmed

image

image 1 :

Immunofluorescent analysis of Phalloidin (orange) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

image 2 :

Immunofluorescent analysis of Phalloidin (purple) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

image 3 :

Immunoprecipitation of EGFR was performed on A431 cell lysates prepared in RIPA buffer (native conditions). Antigen-antibody complexes were formed by incubating 500ug of A431 cell lysate in 1ml total volume with 2ug of an EGFR monoclonal antibody (Product # MA5-12880), preincubated with 10ul of protein G sepharose overnight on a rocking platform at 4°C, for at least 1 hour at 4?C. The immune complexes were washed extensively and eluted with 1X SDS sample buffer. Samples were resolved on a SDS-PAGE gel, transferred to a membrane, blocked, and probed with a set of appropriate antibodies and reagents to detect EGFR.

product information

Product Type :

Antibody

Product Name :

EGFR Monoclonal Antibody (225)

Catalog # :

MA5-12880

Quantity :

500 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Non-human primate

Applications :

Immunocytochemistry: 5 µg/mL, Immunoprecipitation: 2 µg/mL

Species :

Human, Non-human primate

Clone :

225

Isotype :

IgG1

Storage :

4° C

Description :

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

Purified EGFR from A431 cells

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunoprecipitation: 2 µg/mL

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2

more info or order :

Invitrogen product webpage