EGFR Monoclonal Antibody (528) | Invitrogen MA5-12875 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Monoclonal Antibody (528)

catalog :

MA5-12875

quantity :

500 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

528

reactivity :

African green monkey, human, mouse, rat, dogs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 54

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 2 western blot; human; 1:10,000; fig 1	Simon N, Antignani A, Sarnovsky R, Hewitt S, Fitzgerald D. Targeting a Cancer-Specific Epitope of the Epidermal Growth Factor Receptor in Triple-Negative Breast Cancer. J Natl Cancer Inst. 2016;108: pubmed publisher
immunoprecipitation; human; 0.8 ug/time western blot; human	Hasegawa Y, Takahashi M, Ariki S, Asakawa D, Tajiri M, Wada Y, et al. Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling. Oncogene. 2015;34:838-45 pubmed publisher
chromatin immunoprecipitation; human	Chou C, Lee H, Tsou P, Chen C, Hsu J, Yamaguchi H, et al. mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes. Sci Signal. 2014;7:rs1 pubmed publisher
blocking or activating experiments; human	Otero C, Linke M, Sanchez P, Gonzalez A, Schaap I. Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization. PLoS ONE. 2013;8:e83086 pubmed publisher
immunocytochemistry; human	Henriksen L, Grandal M, Knudsen S, van Deurs B, Grøvdal L. Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands. PLoS ONE. 2013;8:e58148 pubmed publisher
flow cytometry; human	Ryu S, Lee S, Yang Y, Song S, Kim J, Choi E, et al. Intracytoplasmic epidermal growth factor receptor shows poor response to the cetuximab antitumor effect in irradiated non-small cell lung cancer cell lines. Lung Cancer. 2012;77:482-7 pubmed publisher
chromatin immunoprecipitation; human	Han W, Carpenter R, Cao X, Lo H. STAT1 gene expression is enhanced by nuclear EGFR and HER2 via cooperation with STAT3. Mol Carcinog. 2013;52:959-69 pubmed publisher
ELISA; human; 0.3 ug/ml and 1.25	Schwenk J, Poetz O, Zeillinger R, Joos T. A Miniaturized Ligand Binding Assay for EGFR. Int J Proteomics. 2012;2012:247059 pubmed publisher
immunoprecipitation; human; 1.5 ug	Siegemund M, Pollak N, Seifert O, Wahl K, Hanak K, Vogel A, et al. Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity. Cell Death Dis. 2012;3:e295 pubmed publisher
flow cytometry; human	Sak M, Breen K, Rønning S, Pedersen N, Bertelsen V, Stang E, et al. The oncoprotein ErbB3 is endocytosed in the absence of added ligand in a clathrin-dependent manner. Carcinogenesis. 2012;33:1031-9 pubmed publisher
blocking or activating experiments; human; 100 ug/ml	Pötz O, Schneiderhan Marra N, Henzler T, Herget T, Joos T. Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays. Methods Mol Biol. 2012;795:191-202 pubmed publisher
immunoprecipitation; African green monkey western blot; African green monkey	Yang X, Guo Z, Sun F, Li W, Alfano A, Shimelis H, et al. Novel membrane-associated androgen receptor splice variant potentiates proliferative and survival responses in prostate cancer cells. J Biol Chem. 2011;286:36152-60 pubmed publisher
western blot; human	Deb T, Zuo A, Wang Y, Barndt R, Cheema A, Sengupta S, et al. Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex. Am J Physiol Cell Physiol. 2011;300:C1139-54 pubmed publisher
immunoprecipitation; rat; 2 ug/ml	Poindexter N, Williams R, Powis G, Jen E, Caudle A, Chada S, et al. IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes. Exp Dermatol. 2010;19:714-22 pubmed publisher
western blot; human	Chou C, Jing N, Yamaguchi H, Tsou P, Lee H, Chen C, et al. High speed digital protein interaction analysis using microfluidic single molecule detection system. Lab Chip. 2010;10:1793-8 pubmed publisher
chromatin immunoprecipitation; human immunocytochemistry; human western blot; human	Liu H, Hsu P, Lai M, Chang H, Ho C, Cheng H, et al. An unusual function of RON receptor tyrosine kinase as a transcriptional regulator in cooperation with EGFR in human cancer cells. Carcinogenesis. 2010;31:1456-64 pubmed publisher
immunohistochemistry; human; fig 3	Qi H, Zheng X, Yuan X, Pflugfelder S, Li D. Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium. J Cell Physiol. 2010;225:180-5 pubmed publisher
immunoprecipitation; human immunocytochemistry; human	Hsu S, Miller S, Wang Y, Hung M. Nuclear EGFR is required for cisplatin resistance and DNA repair. Am J Transl Res. 2009;1:249-58 pubmed
western blot; human	Zhang Y, Su H, Rahimi M, Tochihara R, Tang C. EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer. Int J Cancer. 2009;125:2021-8 pubmed publisher
flow cytometry; human immunohistochemistry; human western blot; human	Al Nedawi K, Meehan B, Kerbel R, Allison A, Rak J. Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A. 2009;106:3794-9 pubmed publisher
immunoprecipitation; human	Bitler B, Menzl I, Huerta C, Sands B, Knowlton W, Chang A, et al. Intracellular MUC1 peptides inhibit cancer progression. Clin Cancer Res. 2009;15:100-9 pubmed publisher
western blot; human; 1:500	Klessner J, Desai B, Amargo E, Getsios S, Green K. EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2. Mol Biol Cell. 2009;20:328-37 pubmed publisher
immunoprecipitation; human	Nakamura A, Naito M, Tsuruo T, Fujita N. Freud-1/Aki1, a novel PDK1-interacting protein, functions as a scaffold to activate the PDK1/Akt pathway in epidermal growth factor signaling. Mol Cell Biol. 2008;28:5996-6009 pubmed publisher
immunoprecipitation; human; 1:50 immunohistochemistry; human; 1:500 western blot; human; 1:500	Rajagopalan V, Zucker I, Jones J, Carlson M, MA Y. Cardiac ErbB-1/ErbB-2 mutant expression in young adult mice leads to cardiac dysfunction. Am J Physiol Heart Circ Physiol. 2008;295:H543-54 pubmed publisher
western blot; human; 1 ug/ml	Stutz M, Shattuck D, Laederich M, Carraway K, Sweeney C. LRIG1 negatively regulates the oncogenic EGF receptor mutant EGFRvIII. Oncogene. 2008;27:5741-52 pubmed publisher
immunoprecipitation; human	Umebayashi K, Stenmark H, Yoshimori T. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 2008;19:3454-62 pubmed publisher
immunoprecipitation; African green monkey	Xu W, Soga S, Beebe K, Lee M, Kim Y, Trepel J, et al. Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition. Br J Cancer. 2007;97:741-4 pubmed
immunoprecipitation; human western blot; human	Yamasaki F, Zhang D, Bartholomeusz C, Sudo T, Hortobagyi G, Kurisu K, et al. Sensitivity of breast cancer cells to erlotinib depends on cyclin-dependent kinase 2 activity. Mol Cancer Ther. 2007;6:2168-77 pubmed
immunoprecipitation; human; 2 ug/time	Yamasaki F, Johansen M, Zhang D, Krishnamurthy S, Felix E, Bartholomeusz C, et al. Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt. Cancer Res. 2007;67:5779-88 pubmed
flow cytometry; human	Yokoyama H, Ikehara Y, Kodera Y, Ikehara S, Yatabe Y, Mochizuki Y, et al. Molecular basis for sensitivity and acquired resistance to gefitinib in HER2-overexpressing human gastric cancer cell lines derived from liver metastasis. Br J Cancer. 2006;95:1504-13 pubmed
immunoprecipitation; human western blot; human	Faivre E, Lange C. Progesterone receptors upregulate Wnt-1 to induce epidermal growth factor receptor transactivation and c-Src-dependent sustained activation of Erk1/2 mitogen-activated protein kinase in breast cancer cells. Mol Cell Biol. 2007;27:466-80 pubmed
immunoprecipitation; mouse	Crotty T, Cai J, Sakane F, Taketomi A, Prescott S, Topham M. Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling. Proc Natl Acad Sci U S A. 2006;103:15485-90 pubmed
immunoprecipitation; human	Martin N, Mohney R, Dunn S, Das M, Scappini E, O Bryan J. Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling. Mol Pharmacol. 2006;70:1643-53 pubmed
immunocytochemistry; human; 1:500	Funes M, Miller J, Lai C, Carraway K, Sweeney C. The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3. J Biol Chem. 2006;281:19310-9 pubmed
immunoprecipitation; human	Lo H, Ali Seyed M, Wu Y, Bartholomeusz G, Hsu S, Hung M. Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1. J Cell Biochem. 2006;98:1570-83 pubmed
flow cytometry; human	Kim Y, Bhandari R, Cochran J, Kuriyan J, Wittrup K. Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast. Proteins. 2006;62:1026-35 pubmed
flow cytometry; mouse; 10 ug/ml	Whitson K, Whitson S, Red Brewer M, McCoy A, Vitali A, Walker F, et al. Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor. Biochemistry. 2005;44:14920-31 pubmed
immunoprecipitation; human western blot; human	Xia W, Gerard C, Liu L, Baudson N, Ory T, Spector N. Combining lapatinib (GW572016), a small molecule inhibitor of ErbB1 and ErbB2 tyrosine kinases, with therapeutic anti-ErbB2 antibodies enhances apoptosis of ErbB2-overexpressing breast cancer cells. Oncogene. 2005;24:6213-21 pubmed
immunoprecipitation; African green monkey	Xu W, Yuan X, Xiang Z, Mimnaugh E, Marcu M, Neckers L. Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex. Nat Struct Mol Biol. 2005;12:120-6 pubmed
	Benadda S, Nugue M, Koumantou D, Bens M, De Luca M, Pell xe9 O, et al. Activating FcγR function depends on endosomal-signaling platforms. iScience. 2023;26:107055 pubmed publisher
	Zhu H, Cao X, Ali Osman F, Keir S, Lo H. EGFR and EGFRvIII interact with PUMA to inhibit mitochondrial translocalization of PUMA and PUMA-mediated apoptosis independent of EGFR kinase activity. Cancer Lett. 2010;294:101-10 pubmed publisher
	Schmidt M, Hoeller D, Yu J, Furnari F, Cavenee W, Dikic I, et al. Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex. Mol Cell Biol. 2004;24:8981-93 pubmed
	Fan Y, Wong L, Deb T, Johnson G. Ligand regulates epidermal growth factor receptor kinase specificity: activation increases preference for GAB1 and SHC versus autophosphorylation sites. J Biol Chem. 2004;279:38143-50 pubmed
	Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
	Schmidt M, Furnari F, Cavenee W, Bogler O. Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization. Proc Natl Acad Sci U S A. 2003;100:6505-10 pubmed
	Luo X, Gong X, Tang C. Suppression of EGFRvIII-mediated proliferation and tumorigenesis of breast cancer cells by ribozyme. Int J Cancer. 2003;104:716-21 pubmed
	Cordero J, Cozzolino M, Lu Y, Vidal M, Slatopolsky E, Stahl P, et al. 1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor. J Biol Chem. 2002;277:38965-71 pubmed
	Stoll S, Kansra S, Elder J. Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture. J Biol Chem. 2002;277:26839-45 pubmed
	Alper O -, Bergmann Leitner E, Bennett T, Hacker N, Stromberg K, Stetler Stevenson W. Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells. J Natl Cancer Inst. 2001;93:1375-84 pubmed
	Gaudry C, Palka H, Dusek R, Huen A, Khandekar M, Hudson L, et al. Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation. J Biol Chem. 2001;276:24871-80 pubmed
	Tikhomirov O, Carpenter G. Geldanamycin induces ErbB-2 degradation by proteolytic fragmentation. J Biol Chem. 2000;275:26625-31 pubmed
	Tang C, Gong X, Moscatello D, Wong A, Lippman M. Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer. Cancer Res. 2000;60:3081-7 pubmed
	Tang C, Concepcion X, Milan M, Gong X, Montgomery E, Lippman M. Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo. Cancer Res. 1999;59:5315-22 pubmed
	Kiyokawa N, Lee E, Karunagaran D, Lin S, Hung M. Mitosis-specific negative regulation of epidermal growth factor receptor, triggered by a decrease in ligand binding and dimerization, can be overcome by overexpression of receptor. J Biol Chem. 1997;272:18656-65 pubmed

product information

Product Type :

Antibody

Product Name :

EGFR Monoclonal Antibody (528)

Catalog # :

MA5-12875

Quantity :

500 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human

Applications :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:400, Immunoprecipitation: Assay-dependent

Species :

Canine, Human

Clone :

528

Isotype :

IgG2a

Storage :

4° C

Description :

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

Purified EGFR from A431 cells

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:400, Immunoprecipitation: Assay-dependent

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2