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Invitrogen
other brands :
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product type :
antibody
product name :
Prohibitin Monoclonal Antibody (II-14-10), Biotin
catalog :
MA5-12855
quantity :
500 µL
price :
US 508.00
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
II-14-10
reactivity :
human, mouse, rat, chicken, bovine, pigs , Hepacivirus C
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 56
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Wang Y, Guo X, Li S, Zhao Y, Liu Z, Lee W, et al. Prohibitin is involved in the activated internalization and degradation of protease-activated receptor 1. Biochim Biophys Acta. 2014;1843:1393-401 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; 1:400
Begum A, Lin Q, Yu C, Kim Y, Yun Z. Interaction of delta-like 1 homolog (Drosophila) with prohibitins and its impact on tumor cell clonogenicity. Mol Cancer Res. 2014;12:155-64 pubmed publisher
  • western blot; human
Panico K, Forti F. Proteomic, cellular, and network analyses reveal new DUSP3 interactions with nucleolar proteins in HeLa cells. J Proteome Res. 2013;12:5851-66 pubmed publisher
  • flow cytometry; human; 1:100
  • immunohistochemistry; human; 1:100
  • western blot; human; 1:500
Jiang P, Xiang Y, Wang Y, Li S, Wang Y, Hua H, et al. Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer. Int J Clin Exp Pathol. 2013;6:2092-101 pubmed
  • western blot; human
Yurugi H, Tanida S, Akita K, Ishida A, Toda M, Nakada H. Prohibitins function as endogenous ligands for Siglec-9 and negatively regulate TCR signaling upon ligation. Biochem Biophys Res Commun. 2013;434:376-81 pubmed publisher
  • western blot; human
Jourdain A, Koppen M, Wydro M, Rodley C, Lightowlers R, Chrzanowska Lightowlers Z, et al. GRSF1 regulates RNA processing in mitochondrial RNA granules. Cell Metab. 2013;17:399-410 pubmed publisher
  • immunocytochemistry; human; 1:50
Picard C, Pellicelli M, Taheri M, Lavoie J, Doucet R, Wang D, et al. Nuclear accumulation of prohibitin 1 in osteoarthritic chondrocytes down-regulates PITX1 expression. Arthritis Rheum. 2013;65:993-1003 pubmed publisher
  • western blot; mouse
Leanza L, Zoratti M, Gulbins E, Szabo I. Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels. Curr Med Chem. 2012;19:5394-404 pubmed
  • western blot; human; 1:1000
Quast S, Berger A, Buttstädt N, Friebel K, Schönherr R, Eberle J. General Sensitization of melanoma cells for TRAIL-induced apoptosis by the potassium channel inhibitor TRAM-34 depends on release of SMAC. PLoS ONE. 2012;7:e39290 pubmed publisher
  • western blot; human; 1:1000
Fletcher C, Dart D, Sita Lumsden A, Cheng H, Rennie P, Bevan C. Androgen-regulated processing of the oncomir miR-27a, which targets Prohibitin in prostate cancer. Hum Mol Genet. 2012;21:3112-27 pubmed publisher
  • western blot; human
Leanza L, Henry B, Sassi N, Zoratti M, Chandy K, Gulbins E, et al. Inhibitors of mitochondrial Kv1.3 channels induce Bax/Bak-independent death of cancer cells. EMBO Mol Med. 2012;4:577-93 pubmed publisher
  • immunohistochemistry; rat; 1:300
Zhou T, Qin Y, Li Z, Xu H, Zhao Y, Lei F. All-trans retinoic acid treatment is associated with prohibitin expression in renal interstitial fibrosis rats. Int J Mol Sci. 2012;13:2769-82 pubmed publisher
  • western blot; human
Kamiński M, Roth D, Sass S, Sauer S, Krammer P, Gülow K. Manganese superoxide dismutase: a regulator of T cell activation-induced oxidative signaling and cell death. Biochim Biophys Acta. 2012;1823:1041-52 pubmed publisher
  • western blot; mouse
Yurugi H, Tanida S, Ishida A, Akita K, Toda M, Inoue M, et al. Expression of prohibitins on the surface of activated T cells. Biochem Biophys Res Commun. 2012;420:275-80 pubmed publisher
  • western blot; human
Orr S, Boutz D, Wang R, Chronis C, Lea N, Thayaparan T, et al. Proteomic and protein interaction network analysis of human T lymphocytes during cell-cycle entry. Mol Syst Biol. 2012;8:573 pubmed publisher
  • western blot; human
Masgras I, Rasola A, Bernardi P. Induction of the permeability transition pore in cells depleted of mitochondrial DNA. Biochim Biophys Acta. 2012;1817:1860-6 pubmed publisher
  • western blot; human; 1:2000
Sofiadis A, Becker S, Hellman U, Hultin Rosenberg L, Dinets A, Hulchiy M, et al. Proteomic profiling of follicular and papillary thyroid tumors. Eur J Endocrinol. 2012;166:657-67 pubmed publisher
  • immunocytochemistry; human
  • western blot; human; 1:1000
Dart D, Brooke G, Sita Lumsden A, Waxman J, Bevan C. Reducing prohibitin increases histone acetylation, and promotes androgen independence in prostate tumours by increasing androgen receptor activation by adrenal androgens. Oncogene. 2012;31:4588-98 pubmed publisher
  • western blot; human
Minet A, Gaster M. The dynamic equilibrium between ATP synthesis and ATP consumption is lower in isolated mitochondria from myotubes established from type 2 diabetic subjects compared to lean control. Biochem Biophys Res Commun. 2011;409:591-5 pubmed publisher
  • western blot; rat
Zhang J, Ye J, Altafaj A, Cardona M, Bahi N, Llovera M, et al. EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes. PLoS ONE. 2011;6:e17998 pubmed publisher
  • western blot; human; 1:150
Yan Y, Xu H, Peng S, Zhao W, Wang B. Proteome analysis of ultraviolet-B-induced protein expression in vitro human dermal fibroblasts. Photodermatol Photoimmunol Photomed. 2010;26:318-26 pubmed publisher
  • western blot; human
Theiss A, Laroui H, Obertone T, Chowdhury I, Thompson W, Merlin D, et al. Nanoparticle-based therapeutic delivery of prohibitin to the colonic epithelial cells ameliorates acute murine colitis. Inflamm Bowel Dis. 2011;17:1163-76 pubmed publisher
  • western blot; bovine
Lee H, Arnouk H, Sripathi S, Chen P, Zhang R, Bartoli M, et al. Prohibitin as an oxidative stress biomarker in the eye. Int J Biol Macromol. 2010;47:685-90 pubmed publisher
  • immunocytochemistry; human; 1:50
  • western blot; human; 1:200
Sperandio S, Poksay K, Schilling B, Crippen D, Gibson B, Bredesen D. Identification of new modulators and protein alterations in non-apoptotic programmed cell death. J Cell Biochem. 2010;111:1401-12 pubmed publisher
  • immunohistochemistry; rat; 1:100
  • western blot; rat; 1:500
Shen Q, Xu H, Wei L, Chen J, Liu H. Intrauterine growth restriction and postnatal high-protein diet affect the kidneys in adult rats. Nutrition. 2011;27:364-71 pubmed publisher
  • western blot; human
Sofiadis A, Dinets A, Orre L, Branca R, Juhlin C, Foukakis T, et al. Proteomic study of thyroid tumors reveals frequent up-regulation of the Ca2+ -binding protein S100A6 in papillary thyroid carcinoma. Thyroid. 2010;20:1067-76 pubmed publisher
  • western blot; human
Liu H, Hsu P, Lai M, Chang H, Ho C, Cheng H, et al. An unusual function of RON receptor tyrosine kinase as a transcriptional regulator in cooperation with EGFR in human cancer cells. Carcinogenesis. 2010;31:1456-64 pubmed publisher
  • western blot; human
Sassi N, De Marchi U, Fioretti B, Biasutto L, Gulbins E, Franciolini F, et al. An investigation of the occurrence and properties of the mitochondrial intermediate-conductance Ca2+-activated K+ channel mtKCa3.1. Biochim Biophys Acta. 2010;1797:1260-7 pubmed publisher
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse; 1:50
  • western blot; mouse
Jain R, Ghoshal A, Mandal C, Shaha C. Leishmania cell surface prohibitin: role in host-parasite interaction. Cell Microbiol. 2010;12:432-52 pubmed publisher
  • immunohistochemistry; human; 1:100
  • western blot; human; 1:200
He Q, Zhang S, Chu Y, Jia X, Zhao L, Wang X. Separation and identification of differentially expressed nuclear matrix proteins in breast carcinoma forming. Acta Oncol. 2010;49:76-84 pubmed publisher
  • immunocytochemistry; human; 1:300
  • western blot; human; 1:1000
Shi S, Li Q, Liu Q, Xu D, Tang J, Liang Y, et al. Nuclear matrix protein, prohibitin, was down-regulated and translocated from nucleus to cytoplasm during the differentiation of osteosarcoma MG-63 cells induced by ginsenoside Rg1, cinnamic acid, and tanshinone IIA (RCT). J Cell Biochem. 2009;108:926-34 pubmed publisher
  • western blot; human
  • western blot; mouse
Theiss A, Jenkins A, Okoro N, Klapproth J, Merlin D, Sitaraman S. Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin alpha3 expression. Mol Biol Cell. 2009;20:4412-23 pubmed publisher
  • western blot; human
Rothfuss O, Fischer H, Hasegawa T, Maisel M, Leitner P, Miesel F, et al. Parkin protects mitochondrial genome integrity and supports mitochondrial DNA repair. Hum Mol Genet. 2009;18:3832-50 pubmed publisher
  • western blot; human
Proulx D, Aubin E, Lemieux R, Bazin R. Spontaneous internalization of IVIg in activated B cells. Immunol Lett. 2009;124:18-26 pubmed publisher
  • western blot; human
De Marchi U, Sassi N, Fioretti B, Catacuzzeno L, Cereghetti G, Szabo I, et al. Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells. Cell Calcium. 2009;45:509-16 pubmed publisher
  • western blot; human; 1:400
Zhang Z, Jia L, Feng Y, Zheng W. Overexpression of follicle-stimulating hormone receptor facilitates the development of ovarian epithelial cancer. Cancer Lett. 2009;278:56-64 pubmed publisher
  • western blot; rat
Chuang Y, Chen S, Liou C, Lin T, Chang W, Chan S, et al. Contribution of nitric oxide, superoxide anion, and peroxynitrite to activation of mitochondrial apoptotic signaling in hippocampal CA3 subfield following experimental temporal lobe status epilepticus. Epilepsia. 2009;50:731-46 pubmed publisher
  • western blot; Hepacivirus C; 1:1000
Nitahara Kasahara Y, Fukasawa M, Shinkai Ouchi F, Sato S, Suzuki T, Murakami K, et al. Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells. Virology. 2009;383:319-27 pubmed publisher
  • western blot; mouse; 1:1000
Da Cruz S, Martinou J. Purification and proteomic analysis of the mouse liver mitochondrial inner membrane. Methods Mol Biol. 2008;432:101-16 pubmed publisher
  • immunohistochemistry; human
Vesela K, Hulkova H, Hansikova H, Zeman J, Elleder M. Structural analysis of tissues affected by cytochrome C oxidase deficiency due to mutations in the SCO2 gene. APMIS. 2008;116:41-9 pubmed publisher
  • western blot; mouse; 1:500
Schmidt V, Chiariello C, Capilla E, Miller F, Bahou W. Development of hepatocellular carcinoma in Iqgap2-deficient mice is IQGAP1 dependent. Mol Cell Biol. 2008;28:1489-502 pubmed publisher
  • immunohistochemistry; human; 1:500
Sikora J, Dvorakova L, Vlaskova H, Stolnaja L, Betlach J, Spacek J, et al. A case of excessive autophagocytosis with multiorgan involvement and low clinical penetrance. Cesk Patol. 2007;43:93-103 pubmed
  • western blot; human
Wu T, Wu H, Wang Y, Chang T, Chan S, Lin Y, et al. Prohibitin in the pathogenesis of transitional cell bladder cancer. Anticancer Res. 2007;27:895-900 pubmed
  • western blot; rat
Chuang Y, Chen S, Lin T, Liou C, Chang W, Chan S, et al. Upregulation of nitric oxide synthase II contributes to apoptotic cell death in the hippocampal CA3 subfield via a cytochrome c/caspase-3 signaling cascade following induction of experimental temporal lobe status epilepticus in the rat. Neuropharmacology. 2007;52:1263-73 pubmed
  • western blot; rat
Chan J, Cheng H, Chou J, Li F, Dai K, Chan S, et al. Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death. J Biol Chem. 2007;282:4585-600 pubmed
  • western blot; chicken; 1:400
Pucci B, Adams C, Fertala J, Snyder B, Mansfield K, Tafani M, et al. Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activation. J Cell Physiol. 2007;210:609-15 pubmed
  • immunohistochemistry; human; 1:100
  • immunocytochemistry; rat; 1:100
  • western blot; rat; 1:1000
Guo W, Xu H, Chen J, Yang Y, Jin J, Fu R, et al. Prohibitin suppresses renal interstitial fibroblasts proliferation and phenotypic change induced by transforming growth factor-beta1. Mol Cell Biochem. 2007;295:167-77 pubmed
  • immunohistochemistry; human; 1:500
Sikora J, Harzer K, Elleder M. Neurolysosomal pathology in human prosaposin deficiency suggests essential neurotrophic function of prosaposin. Acta Neuropathol. 2007;113:163-75 pubmed
  • chromatin immunoprecipitation; human
Joshi B, Rastogi S, Morris M, Carastro L, DeCook C, Seto E, et al. Differential regulation of human YY1 and caspase 7 promoters by prohibitin through E2F1 and p53 binding sites. Biochem J. 2007;401:155-66 pubmed
  • western blot; human
Tsintzas K, Jewell K, Kamran M, Laithwaite D, Boonsong T, Littlewood J, et al. Differential regulation of metabolic genes in skeletal muscle during starvation and refeeding in humans. J Physiol. 2006;575:291-303 pubmed
  • western blot; rat
Chang A, Chan J, Chou J, Li F, Dai K, Chan S. Heat shock protein 60 in rostral ventrolateral medulla reduces cardiovascular fatality during endotoxaemia in the rat. J Physiol. 2006;574:547-64 pubmed
  • western blot; human
Chan H, Gharbi S, Gaffney P, Cramer R, Waterfield M, Timms J. Proteomic analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine- and lysine-labelling two-dimensional difference gel electrophoresis. Proteomics. 2005;5:2908-26 pubmed
  • western blot; human
Suzuki R, Sakamoto S, Tsutsumi T, Rikimaru A, Tanaka K, Shimoike T, et al. Molecular determinants for subcellular localization of hepatitis C virus core protein. J Virol. 2005;79:1271-81 pubmed
  • western blot; human; fig 5
Li Q, Ching A, Chan B, Chow S, Lim P, Ho T, et al. A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway. J Biol Chem. 2004;279:52106-16 pubmed
Ullmann R, Morbini P, Halbwedl I, Bongiovanni M, Gogg Kammerer M, Papotti M, et al. Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays. J Pathol. 2004;203:798-807 pubmed
Thompson W, Ramalho Santos J, Sutovsky P. Ubiquitination of prohibitin in mammalian sperm mitochondria: possible roles in the regulation of mitochondrial inheritance and sperm quality control. Biol Reprod. 2003;69:254-60 pubmed
image
image 1 :
Invitrogen MA5-12855 image 1
Formalin-fixed, paraffin-embedded human heart stained with Prohibitin antibody using peroxidase-conjugate and DAB chromogen.
image 2 :
Invitrogen MA5-12855 image 2
Formalin-fixed, paraffin-embedded rat heart stained with Prohibitin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of cardiac muscles.
product information
Product Type :
Antibody
Product Name :
Prohibitin Monoclonal Antibody (II-14-10), Biotin
Catalog # :
MA5-12855
Quantity :
500 µL
Price :
US 508.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Chicken, Human, Mouse, Non-human primate, Porcine, Rat
Applications :
Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 0.5-1.0 µg/mL
Species :
Chicken, Human, Mouse, Non-human primate, Porcine, Rat
Clone :
II-14-10
Isotype :
IgG1
Storage :
4° C
Description :
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Immunogen :
Purified recombinant rat prohibitin protein
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 0.5-1.0 µg/mL
Aliases :
BAP 32; Bap32; B-cell receptor-associated protein 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; fa18f07; fb09f05; HEL-215; HEL-S-54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; PHB; phb protein; PHB1; Prohibitin; sr:nyz128; wu:fa18f07; wu:fb09f05
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA