p27Kip1 Monoclonal Antibody (DCS-72.F6) | Invitrogen MA5-12835 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p27Kip1 Monoclonal Antibody (DCS-72.F6)

catalog :

MA5-12835

quantity :

500µL

price :

US 299

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-72.F6

reactivity :

human, mouse, rat, dogs

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 48

Reference
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Nguyen K, Blain S, Gress F, Treem W. Inflammatory mediators of esophagitis alter p27 Kip1 expression in esophageal epithelial cells. J Pediatr Gastroenterol Nutr. 2010;51:556-62 pubmed publisher
Sassen A, Diermeier Daucher S, Sieben M, Ortmann O, Hofstaedter F, Schwarz S, et al. Presence of HER4 associates with increased sensitivity to Herceptin in patients with metastatic breast cancer. Breast Cancer Res. 2009;11:R50 pubmed publisher
Zhang W, Zeng Z, Zhou Y, Xiong W, Fan S, Xiao L, et al. Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis. Acta Biochim Biophys Sin (Shanghai). 2009;41:414-28 pubmed
Hoffmann U, Bergler T, Rihm M, Pace C, Kruger B, Rümmele P, et al. Upregulation of TNF receptor type 2 in human and experimental renal allograft rejection. Am J Transplant. 2009;9:675-86 pubmed publisher
Hu H, Zhang J, Lee H, Kim S, Lu J. Penta-O-galloyl-beta-D-glucose induces S- and G(1)-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1. Carcinogenesis. 2009;30:818-23 pubmed publisher
Cinti C, Vindigni C, Zamparelli A, La Sala D, Epistolato M, Marrelli D, et al. Activated Akt as an indicator of prognosis in gastric cancer. Virchows Arch. 2008;453:449-55 pubmed publisher
Voduc D, Nielsen T, Cheang M, Foulkes W. The combination of high cyclin E and Skp2 expression in breast cancer is associated with a poor prognosis and the basal phenotype. Hum Pathol. 2008;39:1431-7 pubmed publisher
Xia W, Nagase S, Montia A, KALACHIKOV S, Keniry M, Su T, et al. BAF180 is a critical regulator of p21 induction and a tumor suppressor mutated in breast cancer. Cancer Res. 2008;68:1667-74 pubmed publisher
Song Z, Wang Y, Xie L, Zang X, Yin H. Expression of senescence-related genes in human corneal endothelial cells. Mol Vis. 2008;14:161-70 pubmed
Ye L, Dong N, Wang Q, Xu Z, Cai N, Wang H, et al. Progressive changes in hepatoma cells stably transfected with hepatitis B virus X gene. Intervirology. 2008;51:50-8 pubmed publisher
Shi S, Liu C, Pootrakul L, Tang L, Young A, Chen R, et al. Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry. Am J Clin Pathol. 2008;129:358-66 pubmed publisher
James M, Ray A, Leznova D, Blain S. Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol. 2008;28:498-510 pubmed
Kim D. Prognostic implications of cyclin B1, p34cdc2, p27(Kip1) and p53 expression in gastric cancer. Yonsei Med J. 2007;48:694-700 pubmed
Manton K, Leong D, Cool S, Nurcombe V. Disruption of heparan and chondroitin sulfate signaling enhances mesenchymal stem cell-derived osteogenic differentiation via bone morphogenetic protein signaling pathways. Stem Cells. 2007;25:2845-54 pubmed
Skvortsov S, Skvortsova I, Stasyk T, Schiefermeier N, Neher A, Gunkel A, et al. Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines. Mol Cancer Ther. 2007;6:1898-908 pubmed
Wang W, Bergh A, Damber J. Increased expression of CCAAT/enhancer-binding protein beta in proliferative inflammatory atrophy of the prostate: relation with the expression of COX-2, the androgen receptor, and presence of focal chronic inflammation. Prostate. 2007;67:1238-46 pubmed
Kawakami K, Enokida H, Tachiwada T, Nishiyama K, Seki N, Nakagawa M. Increased SKP2 and CKS1 gene expression contributes to the progression of human urothelial carcinoma. J Urol. 2007;178:301-7 pubmed
Wiseman D, Werner S, Crowell P. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. J Pharmacol Exp Ther. 2007;320:1163-70 pubmed
Wu X, Shi P, Hu P, Chen Y, Ding S. N-all-trans-retinoyl-L-proline inhibits metastatic potential of hepatocellular carcinoma cells. Cell Biol Int. 2006;30:672-80 pubmed
Rothschild G, Zhao X, Iavarone A, Lasorella A. E Proteins and Id2 converge on p57Kip2 to regulate cell cycle in neural cells. Mol Cell Biol. 2006;26:4351-61 pubmed
Doetzlhofer A, White P, Lee Y, Groves A, Segil N. Prospective identification and purification of hair cell and supporting cell progenitors from the embryonic cochlea. Brain Res. 2006;1091:282-8 pubmed
Piechocki M, Yoo G, Dibbley S, Amjad E, Lonardo F. Iressa induces cytostasis and augments Fas-mediated apoptosis in acinic cell adenocarcinoma overexpressing HER2/neu. Int J Cancer. 2006;119:441-54 pubmed
Farabegoli F, Ceccarelli C, Santini D, Taffurelli M. Suppressor of cytokine signalling 2 (SOCS-2) expression in breast carcinoma. J Clin Pathol. 2005;58:1046-50 pubmed
Siitonen T, Koistinen P, Savolainen E. Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells. Leuk Res. 2005;29:1335-42 pubmed
Yoo G, Subramanian G, Boinpally R, Iskander A, Shehadeh N, Oliver J, et al. An in vivo evaluation of docetaxel delivered intratumorally in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2005;131:418-29 pubmed
Ito Y, Yoshida H, Motoo Y, Iovanna J, Nakamura Y, Kakudo K, et al. Expression of p8 protein in breast carcinoma; an inverse relationship with apoptosis. Anticancer Res. 2005;25:833-7 pubmed
Mallikarjuna G, Dhanalakshmi S, Singh R, Agarwal C, Agarwal R. Silibinin protects against photocarcinogenesis via modulation of cell cycle regulators, mitogen-activated protein kinases, and Akt signaling. Cancer Res. 2004;64:6349-56 pubmed
Radde Gallwitz K, Pan L, Gan L, Lin X, Segil N, Chen P. Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear. J Comp Neurol. 2004;477:412-21 pubmed
Au N, Cheang M, Huntsman D, Yorida E, Coldman A, Elliott W, et al. Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers. J Pathol. 2004;204:101-9 pubmed
Doetzlhofer A, White P, Johnson J, Segil N, Groves A. In vitro growth and differentiation of mammalian sensory hair cell progenitors: a requirement for EGF and periotic mesenchyme. Dev Biol. 2004;272:432-47 pubmed
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Ozkara S, Corakci A. Significantly decreased P27 expression in endometrial carcinoma compared to complex hyperplasia with atypia (correlation with p53 expression). Pathol Oncol Res. 2004;10:89-97 pubmed
Claudio P, Russo G, Kumar C, Minimo C, Farina A, Tutton S, et al. pRb2/p130, vascular endothelial growth factor, p27(KIP1), and proliferating cell nuclear antigen expression in hepatocellular carcinoma: their clinical significance. Clin Cancer Res. 2004;10:3509-17 pubmed
Farabegoli F, Hermsen M, Ceccarelli C, Santini D, Weiss M, Meijer G, et al. Simultaneous chromosome 1q gain and 16q loss is associated with steroid receptor presence and low proliferation in breast carcinoma. Mod Pathol. 2004;17:449-55 pubmed
Qiu L, Sinniah R, I Hong Hsu S. Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy. J Am Soc Nephrol. 2004;15:79-90 pubmed
Daneshmand M, Parolin D, Hirte H, Major P, Goss G, Stewart D, et al. A pharmacodynamic study of the epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 in metastatic colorectal cancer patients. Clin Cancer Res. 2003;9:2457-64 pubmed
Dubowy R, Feinberg R, Keefe D, Doncel G, Williams S, McSweet J, et al. Improved endometrial assessment using cyclin E and p27. Fertil Steril. 2003;80:146-56 pubmed
Claudio P, Zamparelli A, Garcia F, Claudio L, Ammirati G, Farina A, et al. Expression of cell-cycle-regulated proteins pRb2/p130, p107, p27(kip1), p53, mdm-2, and Ki-67 (MIB-1) in prostatic gland adenocarcinoma. Clin Cancer Res. 2002;8:1808-15 pubmed
Chen P, Johnson J, Zoghbi H, Segil N. The role of Math1 in inner ear development: Uncoupling the establishment of the sensory primordium from hair cell fate determination. Development. 2002;129:2495-505 pubmed
Lu Y, Zi X, Zhao Y, Mascarenhas D, Pollak M. Insulin-like growth factor-I receptor signaling and resistance to trastuzumab (Herceptin). J Natl Cancer Inst. 2001;93:1852-7 pubmed
Yaroslavskiy B, Stolz D, Watkins S, Alber S, Bradbury N, Steinman R. p27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes. Mol Med. 2001;7:49-58 pubmed
Masciullo V, Ferrandina G, Pucci B, Fanfani F, Lovergine S, Palazzo J, et al. p27Kip1 expression is associated with clinical outcome in advanced epithelial ovarian cancer: multivariate analysis. Clin Cancer Res. 2000;6:4816-22 pubmed
Tsutsui T, Hesabi B, Moons D, Pandolfi P, Hansel K, Koff A, et al. Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity. Mol Cell Biol. 1999;19:7011-9 pubmed

product information

Product Type :

Antibody

Product Name :

p27Kip1 Monoclonal Antibody (DCS-72.F6)

Catalog # :

MA5-12835

Quantity :

500µL

Price :

US 299

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

Flow Cytometry: 2 µg/test, Immunofluorescence: Assay Dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL

Species :

Canine, Human, Mouse, Rat

Clone :

DCS-72.F6

Isotype :

IgG1, kappa

Storage :

4° C

Description :

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Immunogen :

Mouse recombinant p27 protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 2 µg/test, Immunofluorescence: Assay Dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL

Aliases :

AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; cyclin-dependent kinase inhibitor p27/Kip1; KIP1; MEN1B; MEN4; p27; P27 CDKN1B; p27(KIP1); p27Kip1; putative cyclin-dependent kinase inhibitor p27