Tau Monoclonal Antibody (TAU-5), Biotin | Invitrogen MA5-12805 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Tau Monoclonal Antibody (TAU-5), Biotin

catalog :

MA5-12805

quantity :

500 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

Tau-5

reactivity :

human, mouse, rat, bovine, domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 53

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; loading ...; fig 5a	Zhang X, Zou L, Meng L, Xiong M, Pan L, Chen G, et al. Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. elife. 2021;10: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4b	Zhang Z, Obianyo O, Dall E, Du Y, Fu H, Liu X, et al. Inhibition of delta-secretase improves cognitive functions in mouse models of Alzheimer's disease. Nat Commun. 2017;8:14740 pubmed publisher
western blot; rat	Du L, Xie J, Cheng X, Li X, Kong F, Jiang X, et al. Activation of sirtuin 1 attenuates cerebral ventricular streptozotocin-induced tau hyperphosphorylation and cognitive injuries in rat hippocampi. Age (Dordr). 2014;36:613-23 pubmed publisher
immunoprecipitation; human western blot; human; 1:5000	Cameron A, Giacomozzi B, Joyce J, Gray A, Graham D, Ousson S, et al. Generation and characterization of a rabbit monoclonal antibody site-specific for tau O-GlcNAcylated at serine 400. FEBS Lett. 2013;587:3722-8 pubmed publisher
western blot; mouse	Li X, Du L, Cheng X, Jiang X, Zhang Y, Lv B, et al. Glycation exacerbates the neuronal toxicity of ?-amyloid. Cell Death Dis. 2013;4:e673 pubmed publisher
western blot; human; 1:200	Park Y, Ko J, Jang Y, Kwon Y. Activation of AMP-activated protein kinase alleviates homocysteine-mediated neurotoxicity in SH-SY5Y cells. Neurochem Res. 2013;38:1561-71 pubmed publisher
western blot; mouse; 1:500	Tackenberg C, Grinschgl S, Trutzel A, Santuccione A, Frey M, Konietzko U, et al. NMDA receptor subunit composition determines beta-amyloid-induced neurodegeneration and synaptic loss. Cell Death Dis. 2013;4:e608 pubmed publisher
western blot; mouse	Cheng X, Zhao K, Jiang X, Du L, Li X, Ma Z, et al. Nmnat2 attenuates Tau phosphorylation through activation of PP2A. J Alzheimers Dis. 2013;36:185-95 pubmed publisher
western blot; human; 1:1000	Yu G, Yan T, Feng Y, Liu X, Xia Y, Luo H, et al. Ser9 phosphorylation causes cytoplasmic detention of I2PP2A/SET in Alzheimer disease. Neurobiol Aging. 2013;34:1748-58 pubmed publisher
western blot; rat; 1:1000	Hebron M, Algarzae N, Lonskaya I, Moussa C. Fractalkine signaling and Tau hyper-phosphorylation are associated with autophagic alterations in lentiviral Tau and A?1-42 gene transfer models. Exp Neurol. 2014;251:127-38 pubmed publisher
western blot; mouse	Bhoiwala D, Koleilat I, Qian J, Beyer B, Hushmendy S, Mathew A, et al. Overexpression of RCAN1 isoform 4 in mouse neurons leads to a moderate behavioral impairment. Neurol Res. 2013;35:79-89 pubmed publisher
western blot; rat; 1:5000	Zussy C, Brureau A, Keller E, Marchal S, Blayo C, Delair B, et al. Alzheimer's disease related markers, cellular toxicity and behavioral deficits induced six weeks after oligomeric amyloid-? peptide injection in rats. PLoS ONE. 2013;8:e53117 pubmed publisher
immunocytochemistry; human; 1:100 western blot; human; 1:1000	Liu X, Song J, Jiang Q, Wang Q, Tian Q, Wang J. Expression of the hyperphosphorylated tau attenuates ER stress-induced apoptosis with upregulation of unfolded protein response. Apoptosis. 2012;17:1039-49 pubmed publisher
western blot; mouse	Li X, Xie J, Jiang X, Lv B, Cheng X, Du L, et al. Methylglyoxal induces tau hyperphosphorylation via promoting AGEs formation. Neuromolecular Med. 2012;14:338-48 pubmed publisher
western blot; mouse	Tweedie D, Ferguson R, Fishman K, Frankola K, van Praag H, Holloway H, et al. Tumor necrosis factor-? synthesis inhibitor 3,6'-dithiothalidomide attenuates markers of inflammation, Alzheimer pathology and behavioral deficits in animal models of neuroinflammation and Alzheimer's disease. J Neuroinflammation. 2012;9:106 pubmed publisher
western blot; rat	Yao Z, Zhang J, Xie X. Enriched environment prevents cognitive impairment and tau hyperphosphorylation after chronic cerebral hypoperfusion. Curr Neurovasc Res. 2012;9:176-84 pubmed
western blot; human; 1:1000	Liu Z, Fu Z, Song J, Zhang J, Wei Y, Chu J, et al. Bip enhanced the association of GSK-3? with tau during ER stress both in vivo and in vitro. J Alzheimers Dis. 2012;29:727-40 pubmed publisher
western blot; rat	Li G, Cai F, Yan W, Li C, Wang J. A proteomic analysis of MCLR-induced neurotoxicity: implications for Alzheimer's disease. Toxicol Sci. 2012;127:485-95 pubmed publisher
western blot; rat; 1:1000 western blot; human; 1:1000	Jones N, Nguyen T, Corcoran N, Velakoulis D, Chen T, Grundy R, et al. Targeting hyperphosphorylated tau with sodium selenate suppresses seizures in rodent models. Neurobiol Dis. 2012;45:897-901 pubmed publisher
western blot; human	Kim J, Park Y, Jang Y, Kwon Y. AMPK activation inhibits apoptosis and tau hyperphosphorylation mediated by palmitate in SH-SY5Y cells. Brain Res. 2011;1418:42-51 pubmed publisher
western blot; rat; 1:1000	Wei W, Liu Y, Zhang C, Wang Q, Wei Z, Mousseau D, et al. Folate/vitamin-B12 prevents chronic hyperhomocysteinemia-induced tau hyperphosphorylation and memory deficits in aged rats. J Alzheimers Dis. 2011;27:639-50 pubmed publisher
western blot; human; 1:1000	Fu Z, Yang Y, Song J, Jiang Q, Lin Z, Wang Q, et al. LiCl attenuates thapsigargin-induced tau hyperphosphorylation by inhibiting GSK-3? in vivo and in vitro. J Alzheimers Dis. 2010;21:1107-17 pubmed
immunohistochemistry; human western blot; human immunohistochemistry; mouse western blot; mouse	Chen L, Xiong Y, Kong F, Qu M, Wang Q, Chen X, et al. Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling. J Neurochem. 2012;120:157-64 pubmed publisher
western blot; human	Li X, Lv B, Xie J, Liu J, Zhou X, Wang J. AGEs induce Alzheimer-like tau pathology and memory deficit via RAGE-mediated GSK-3 activation. Neurobiol Aging. 2012;33:1400-10 pubmed publisher
western blot; human; 1:1000	Yu G, Li Y, Tian Q, Liu R, Wang Q, Wang J, et al. Berberine attenuates calyculin A-induced cytotoxicity and Tau hyperphosphorylation in HEK293 cells. J Alzheimers Dis. 2011;24:525-35 pubmed publisher
western blot; rat; 1:1000	Zhang Z, Zhao R, Qi J, Wen S, Tang Y, Wang D. Inhibition of glycogen synthase kinase-3? by Angelica sinensis extract decreases ?-amyloid-induced neurotoxicity and tau phosphorylation in cultured cortical neurons. J Neurosci Res. 2011;89:437-47 pubmed publisher
immunocytochemistry; mouse	Dachsel J, Behrouz B, Yue M, Beevers J, Melrose H, Farrer M. A comparative study of Lrrk2 function in primary neuronal cultures. Parkinsonism Relat Disord. 2010;16:650-5 pubmed publisher
western blot; rat	Yuzwa S, Yadav A, Skorobogatko Y, Clark T, Vosseller K, Vocadlo D. Mapping O-GlcNAc modification sites on tau and generation of a site-specific O-GlcNAc tau antibody. Amino Acids. 2011;40:857-68 pubmed publisher
western blot; human	Park Y, Jang Y, Kwon Y. Protective effect of isoflavones against homocysteine-mediated neuronal degeneration in SH-SY5Y cells. Amino Acids. 2010;39:785-94 pubmed publisher
western blot; human	Liu X, Liao K, Liu R, Wang H, Zhang Y, Zhang Q, et al. Tau dephosphorylation potentiates apoptosis by mechanisms involving a failed dephosphorylation/activation of Bcl-2. J Alzheimers Dis. 2010;19:953-62 pubmed publisher
western blot; mouse; 1:200	Kulic L, Wollmer M, Rhein V, Pagani L, Kuehnle K, Cattepoel S, et al. Combined expression of tau and the Harlequin mouse mutation leads to increased mitochondrial dysfunction, tau pathology and neurodegeneration. Neurobiol Aging. 2011;32:1827-38 pubmed publisher
western blot; human	Hundelt M, Fath T, Selle K, Oesterwind K, Jordan J, Schultz C, et al. Altered phosphorylation but no neurodegeneration in a mouse model of tau hyperphosphorylation. Neurobiol Aging. 2011;32:991-1006 pubmed publisher
western blot; human	Park Y, Jang Y, Kwon Y. Isoflavones prevent endoplasmic reticulum stress-mediated neuronal degeneration by inhibiting tau hyperphosphorylation in SH-SY5Y cells. J Med Food. 2009;12:528-35 pubmed publisher
western blot; rat; 1:1000	Zhang J, Peng C, Shi H, Wang S, Wang Q, Wang J. Inhibition of autophagy causes tau proteolysis by activating calpain in rat brain. J Alzheimers Dis. 2009;16:39-47 pubmed publisher
western blot; human; 1:1000	Liu X, Zhu L, Zhang Q, Shi H, Wang S, Wang Q, et al. Estradiol attenuates tau hyperphosphorylation induced by upregulation of protein kinase-A. Neurochem Res. 2008;33:1811-20 pubmed publisher
western blot; mouse	Shi H, Zhu L, Wang S, Liu X, Tian Q, Zhang Q, et al. 17beta-estradiol attenuates glycogen synthase kinase-3beta activation and tau hyperphosphorylation in Akt-independent manner. J Neural Transm (Vienna). 2008;115:879-88 pubmed publisher
immunohistochemistry; mouse; 1:5 western blot; mouse; 1:200	Bai F, Xi J, Andley U. Up-regulation of tau, a brain microtubule-associated protein, in lens cortical fractions of aged alphaA-, alphaB-, and alphaA/B-crystallin knockout mice. Mol Vis. 2007;13:1589-600 pubmed
western blot; human; 1:200	Liu G, Zhang Y, Yao X, Zhang C, Fang J, Wang Q, et al. Activation of glycogen synthase kinase-3 inhibits protein phosphatase-2A and the underlying mechanisms. Neurobiol Aging. 2008;29:1348-58 pubmed
western blot; human; 1:2000	Ren Q, Liao X, Chen X, Liu G, Wang J. Effects of tau phosphorylation on proteasome activity. FEBS Lett. 2007;581:1521-8 pubmed
western blot; human; 1:200	Ren Q, Liao X, Wang Z, Qu Z, Wang J. The involvement of glycogen synthase kinase-3 and protein phosphatase-2A in lactacystin-induced tau accumulation. FEBS Lett. 2006;580:2503-11 pubmed
western blot; rat; 1:250	Esposito G, De Filippis D, Carnuccio R, Izzo A, Iuvone T. The marijuana component cannabidiol inhibits beta-amyloid-induced tau protein hyperphosphorylation through Wnt/beta-catenin pathway rescue in PC12 cells. J Mol Med (Berl). 2006;84:253-8 pubmed
immunoprecipitation; human	Dalfo E, Albasanz J, Rodriguez A, Martín M, Ferrer I. Abnormal group I metabotropic glutamate receptor expression and signaling in the frontal cortex in Pick disease. J Neuropathol Exp Neurol. 2005;64:638-47 pubmed
western blot; human	Hu Lieskovan S, Zhang J, Wu L, Shimada H, Schofield D, Triche T. EWS-FLI1 fusion protein up-regulates critical genes in neural crest development and is responsible for the observed phenotype of Ewing's family of tumors. Cancer Res. 2005;65:4633-44 pubmed
western blot; human; 1:2000	Zhang Y, Xu Y, Chen X, Wang X, Wang J. Nitration and oligomerization of tau induced by peroxynitrite inhibit its microtubule-binding activity. FEBS Lett. 2005;579:2421-7 pubmed
	Steuer E, Kemper L, Hlynialuk C, Leinonen Wright K, Montonye M, Lapcinski I, et al. Blocking Site-Specific Cleavage of Human Tau Delays Progression of Disease-Related Phenotypes in Genetically Matched Tau-Transgenic Mice Modeling Frontotemporal Dementia. J Neurosci. 2022;42:4737-4754 pubmed publisher
	Liu P, Smith B, Huang E, Mahesh A, Vonsattel J, Petersen A, et al. A soluble truncated tau species related to cognitive dysfunction and caspase-2 is elevated in the brain of Huntington's disease patients. Acta Neuropathol Commun. 2019;7:111 pubmed publisher
	Liu Y, Cao L, Zhang X, Liang Y, Xu Y, Zhu C. Memantine Differentially Regulates Tau Phosphorylation Induced by Chronic Restraint Stress of Varying Duration in Mice. Neural Plast. 2019;2019:4168472 pubmed publisher
	Lin D, Cao L, Wang Z, Li J, Washington J, Zuo Z. Lidocaine attenuates cognitive impairment after isoflurane anesthesia in old rats. Behav Brain Res. 2012;228:319-27 pubmed publisher
	Patzke H, Maddineni U, Ayala R, Morabito M, Volker J, Dikkes P, et al. Partial rescue of the p35-/- brain phenotype by low expression of a neuronal-specific enolase p25 transgene. J Neurosci. 2003;23:2769-78 pubmed
	Stanford P, Shepherd C, Halliday G, Brooks W, Schofield P, Brodaty H, et al. Mutations in the tau gene that cause an increase in three repeat tau and frontotemporal dementia. Brain. 2003;126:814-26 pubmed
	Xu G, Gonzales V, Borchelt D. Rapid detection of protein aggregates in the brains of Alzheimer patients and transgenic mouse models of amyloidosis. Alzheimer Dis Assoc Disord. 2002;16:191-5 pubmed
	LoPresti P, Konat G. Hydrogen peroxide induces transient dephosphorylation of tau protein in cultured rat oligodendrocytes. Neurosci Lett. 2001;311:142-4 pubmed
	Kins S, Crameri A, Evans D, Hemmings B, Nitsch R, Gotz J. Reduced protein phosphatase 2A activity induces hyperphosphorylation and altered compartmentalization of tau in transgenic mice. J Biol Chem. 2001;276:38193-200 pubmed

product information

Product Type :

Antibody

Product Name :

Tau Monoclonal Antibody (TAU-5), Biotin

Catalog # :

MA5-12805

Quantity :

500 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Ovine, Rat

Applications :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Bovine, Human, Mouse, Ovine, Rat

Clone :

TAU-5

Isotype :

IgG1

Storage :

4° C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

Purified bovine microtubule-associated proteins

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)