Collagen II Monoclonal Antibody (2B1.5), Biotin | Invitrogen MA5-12786 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Collagen II Monoclonal Antibody (2B1.5), Biotin

catalog :

MA5-12786

quantity :

500 µL

price :

US 454.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

2B1.5

reactivity :

domestic goat, guinea pig, human, mouse, rat, chicken, bovine, pigs , domestic sheep, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 52

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000	Belluoccio D, Rowley L, Little C, Bateman J. Maintaining mRNA integrity during decalcification of mineralized tissues. PLoS ONE. 2013;8:e58154 pubmed publisher
immunohistochemistry; mouse; 1:100	Kalwani N, Ong C, Lysaght A, Haward S, McKinley G, Stankovic K. Quantitative polarized light microscopy of unstained mammalian cochlear sections. J Biomed Opt. 2013;18:26021 pubmed publisher
immunohistochemistry; mouse	Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, et al. FGF23 suppresses chondrocyte proliferation in the presence of soluble ?-Klotho both in vitro and in vivo. J Biol Chem. 2013;288:2414-27 pubmed publisher
immunohistochemistry; rat; 1:50	Rui Y, Lui P, Wong Y, Tan Q, Chan K. Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy. Stem Cells Dev. 2013;22:1076-85 pubmed publisher
immunocytochemistry; mouse; 1:100	Katebi N, Kolpakova Hart E, Lin C, Olsen B. The mouse palate and its cellular responses to midpalatal suture expansion forces. Orthod Craniofac Res. 2012;15:148-58 pubmed publisher
immunocytochemistry; rat; 1:100	Kang E, Ha K, Kim Y. Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes. J Korean Med Sci. 2012;27:586-93 pubmed publisher
immunohistochemistry; domestic rabbit; 1:100	Suwannaloet W, Laupattarakasem W, Sukon P, Ong Chai S, Laupattarakasem P. Combined effect of subchondral drilling and hyaluronic acid with/without diacerein in full-thickness articular cartilage lesion in rabbits. ScientificWorldJournal. 2012;2012:310745 pubmed publisher
immunohistochemistry; domestic rabbit; 1:500	Zhang L, Yuan T, Guo L, Zhang X. An in vitro study of collagen hydrogel to induce the chondrogenic differentiation of mesenchymal stem cells. J Biomed Mater Res A. 2012;100:2717-25 pubmed publisher
immunohistochemistry; mouse; 1:400	Masago Y, Hosoya A, Kawasaki K, Kawano S, Nasu A, Toguchida J, et al. The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation. J Cell Sci. 2012;125:1118-28 pubmed publisher
immunohistochemistry; mouse	Koshimizu T, Kawai M, Kondou H, Tachikawa K, Sakai N, Ozono K, et al. Vinculin functions as regulator of chondrogenesis. J Biol Chem. 2012;287:15760-75 pubmed publisher
immunohistochemistry; bovine	Kuo Y, Wang C. Cartilage regeneration by culturing chondrocytes in scaffolds grafted with TATVHL peptide. Colloids Surf B Biointerfaces. 2012;93:235-40 pubmed publisher
immunohistochemistry; bovine	Kuo Y, Chung C. Chondrogenesis in scaffolds with surface modification of elastin and poly-L-lysine. Colloids Surf B Biointerfaces. 2012;93:85-91 pubmed publisher
immunohistochemistry; pigs ; 1:200	Lyu S, Kuo Y, Lin M, Hsieh W, Chuang C. Application of albumin-grafted scaffolds to promote neocartilage formation. Colloids Surf B Biointerfaces. 2012;91:296-301 pubmed publisher
immunohistochemistry; bovine	Kuo Y, Wang C. Surface modification with peptide for enhancing chondrocyte adhesion and cartilage regeneration in porous scaffolds. Colloids Surf B Biointerfaces. 2011;84:63-70 pubmed publisher
immunohistochemistry; rat	Karaoz E, Okcu A, Saglam O, Genc Z, Ayhan S, Kasap M. Pancreatic islet derived stem cells can express co-stimulatory molecules of antigen-presenting cells. Transplant Proc. 2010;42:3663-70 pubmed publisher
immunohistochemistry; bovine; 1:100	Kuo Y, Tsai Y. Heparin-conjugated scaffolds with pore structure of inverted colloidal crystals for cartilage regeneration. Colloids Surf B Biointerfaces. 2011;82:616-23 pubmed publisher
immunohistochemistry; rat	Adas G, Arikan S, Karatepe O, Kemik O, Ayhan S, Karaoz E, et al. Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study). Langenbecks Arch Surg. 2011;396:115-26 pubmed publisher
immunocytochemistry; human; 1:1	Karaoz E, Okçu A, Gacar G, Sağlam O, Yuruker S, Kenar H. A comprehensive characterization study of human bone marrow mscs with an emphasis on molecular and ultrastructural properties. J Cell Physiol. 2011;226:1367-82 pubmed publisher
immunohistochemistry; human	Hildner F, Peterbauer A, Wolbank S, Nürnberger S, Marlovits S, Redl H, et al. FGF-2 abolishes the chondrogenic effect of combined BMP-6 and TGF-beta in human adipose derived stem cells. J Biomed Mater Res A. 2010;94:978-87 pubmed publisher
immunohistochemistry; bovine; 1:100	Kuo Y, Tsai Y. Inverted colloidal crystal scaffolds for uniform cartilage regeneration. Biomacromolecules. 2010;11:731-9 pubmed publisher
immunohistochemistry; domestic sheep	Saw K, Hussin P, Loke S, Azam M, Chen H, Tay Y, et al. Articular cartilage regeneration with autologous marrow aspirate and hyaluronic Acid: an experimental study in a goat model. Arthroscopy. 2009;25:1391-400 pubmed publisher
immunocytochemistry; human	Karaoz E, Doğan B, Aksoy A, Gacar G, Akyuz S, Ayhan S, et al. Isolation and in vitro characterisation of dental pulp stem cells from natal teeth. Histochem Cell Biol. 2010;133:95-112 pubmed publisher
immunohistochemistry; human	Knutsson J, Bagger Sjoback D, von Unge M. Collagen type distribution in the healthy human tympanic membrane. Otol Neurotol. 2009;30:1225-9 pubmed publisher
immunocytochemistry; rat	Karaoz E, Aksoy A, Ayhan S, Sariboyaci A, Kaymaz F, Kasap M. Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers. Histochem Cell Biol. 2009;132:533-46 pubmed publisher
immunohistochemistry; guinea pig	Bernstein P, Dong M, Graupner S, Graupher S, Corbeil D, Gelinsky M, et al. Sox9 expression of alginate-encapsulated chondrocytes is stimulated by low cell density. J Biomed Mater Res A. 2009;91:910-8 pubmed publisher
immunohistochemistry; domestic rabbit	Pfeffer B, Bernstein S, Bartels S. Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eye. Graefes Arch Clin Exp Ophthalmol. 2009;247:193-205 pubmed publisher
immunohistochemistry; domestic goat; 1:200	Wong M, Qin L, Lee K, Leung K. Articular cartilage increases transition zone regeneration in bone-tendon junction healing. Clin Orthop Relat Res. 2009;467:1092-100 pubmed publisher
immunohistochemistry; bovine	Kuo Y, Hsu Y. Tissue-engineered polyethylene oxide/chitosan scaffolds as potential substitutes for articular cartilage. J Biomed Mater Res A. 2009;91:277-87 pubmed publisher
immunohistochemistry; mouse; 1:100	Kretlow J, Jin Y, Liu W, Zhang W, Hong T, Zhou G, et al. Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol. 2008;9:60 pubmed publisher
immunohistochemistry; rat; 1:100	Lui P, Fu S, Chan L, Hung L, Chan K. Chondrocyte phenotype and ectopic ossification in collagenase-induced tendon degeneration. J Histochem Cytochem. 2009;57:91-100 pubmed publisher
immunohistochemistry; rat; 1:200	Wang Z, Yang Z, He X, Wang L, Li L, Tu J. Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytes in vitro. Acta Pharmacol Sin. 2008;29:1215-26 pubmed publisher
immunocytochemistry; rat	Yingst S, Bloxham K, Warner L, Brown R, Cole J, Kenoyer L, et al. Characterization of collagenous matrix assembly in a chondrocyte model system. J Biomed Mater Res A. 2009;90:247-55 pubmed publisher
immunohistochemistry; human	Hui T, Cheung K, Cheung W, Chan D, Chan B. In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration. Biomaterials. 2008;29:3201-12 pubmed publisher
immunohistochemistry; pigs ; 1:100	Bilgen B, Orsini E, Aaron R, Ciombor D. FBS suppresses TGF-beta1-induced chondrogenesis in synoviocyte pellet cultures while dexamethasone and dynamic stimuli are beneficial. J Tissue Eng Regen Med. 2007;1:436-42 pubmed publisher
immunohistochemistry; mouse	Hou B, Fukai N, Olsen B. Mechanical force-induced midpalatal suture remodeling in mice. Bone. 2007;40:1483-93 pubmed
western blot; rat; 2 ug/ml	Schreiweis M, Butler J, Kulkarni N, Knierman M, Higgs R, Halladay D, et al. A proteomic analysis of adult rat bone reveals the presence of cartilage/chondrocyte markers. J Cell Biochem. 2007;101:466-76 pubmed
immunohistochemistry; human; 1:10	Romito L, Ameer G. Mechanical interlocking of engineered cartilage to an underlying polymeric substrate: towards a biohybrid tissue equivalent. Ann Biomed Eng. 2006;34:737-47 pubmed
western blot; human	Lin Y, Luo E, Chen X, Liu L, Qiao J, Yan Z, et al. Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo. J Cell Mol Med. 2005;9:929-39 pubmed
immunohistochemistry; human; 1:500	Hecht J, Hayes E, Haynes R, Cole W, Long R, Farach Carson M, et al. Differentiation-induced loss of heparan sulfate in human exostosis derived chondrocytes. Differentiation. 2005;73:212-21 pubmed
immunohistochemistry; mouse	Wang Y, Xiao R, Yang F, Karim B, Iacovelli A, Cai J, et al. Abnormalities in cartilage and bone development in the Apert syndrome FGFR2(+/S252W) mouse. Development. 2005;132:3537-48 pubmed
immunohistochemistry; human	Peker S, Kurtkaya Yapicier O, Kilic T, Pamir M. Microsurgical anatomy of the lateral walls of the pituitary fossa. Acta Neurochir (Wien). 2005;147:641-8; discussion 649 pubmed
western blot; mouse	Ortega N, Behonick D, Colnot C, Cooper D, Werb Z. Galectin-3 is a downstream regulator of matrix metalloproteinase-9 function during endochondral bone formation. Mol Biol Cell. 2005;16:3028-39 pubmed
western blot; domestic rabbit	Kose G, Korkusuz F, Ozkul A, Soysal Y, Ozdemir T, Yildiz C, et al. Tissue engineered cartilage on collagen and PHBV matrices. Biomaterials. 2005;26:5187-97 pubmed
immunohistochemistry; human; 1:100	Bobryshev Y. Transdifferentiation of smooth muscle cells into chondrocytes in atherosclerotic arteries in situ: implications for diffuse intimal calcification. J Pathol. 2005;205:641-50 pubmed
	Li W, Zhang X, Xi X, Li Y, Quan H, Liu S, et al. PLK2 modulation of enriched TAp73 affects osteogenic differentiation and prognosis in human osteosarcoma. Cancer Med. 2020;9:4371-4385 pubmed publisher
	Song K, Nam Y, Luo X, Qi X, Tan W, Huang G, et al. Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature. 2012;485:599-604 pubmed publisher
	Chang C, Ramaswamy G, Serra R. Depletion of primary cilia in articular chondrocytes results in reduced Gli3 repressor to activator ratio, increased Hedgehog signaling, and symptoms of early osteoarthritis. Osteoarthritis Cartilage. 2012;20:152-61 pubmed publisher
	Locker M, Kellermann O, Boucquey M, Khun H, Huerre M, Poliard A. Paracrine and autocrine signals promoting full chondrogenic differentiation of a mesoblastic cell line. J Bone Miner Res. 2004;19:100-10 pubmed
	Nakayama N, Duryea D, Manoukian R, Chow G, Han C. Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells. J Cell Sci. 2003;116:2015-28 pubmed
	Dabovic B, Chen Y, Colarossi C, Zambuto L, Obata H, Rifkin D. Bone defects in latent TGF-beta binding protein (Ltbp)-3 null mice; a role for Ltbp in TGF-beta presentation. J Endocrinol. 2002;175:129-41 pubmed
	Wang W, Wang Y, Reginato A, Plotkina S, Gridley T, Olsen B. Growth defect in Grg5 null mice is associated with reduced Ihh signaling in growth plates. Dev Dyn. 2002;224:79-89 pubmed
	Odelberg S, Kollhoff A, Keating M. Dedifferentiation of mammalian myotubes induced by msx1. Cell. 2000;103:1099-109 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human lung stained with Collagen II antibody using peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.

product information

Product Type :

Antibody

Product Name :

Collagen II Monoclonal Antibody (2B1.5), Biotin

Catalog # :

MA5-12786

Quantity :

500 µL

Price :

US 454.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Chicken, Human, Mouse, Rat

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): 1-2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Bovine, Chicken, Human, Mouse, Rat

Clone :

2B1.5

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Immunogen :

A purified preparation of lathyritic type II collagen from embryonic chicken sternum

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): 1-2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha 1 type IIA collagen precursor; alpha collagen type II; alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CB11; CG2A1A; CGA; Chondrocalcin; COL11A3; Col2; Col2a; col2a1; Col2a-1; col2a1.L; col2a1a; col2a1-a; col2a1-b; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen type II alpha 1 chain; collagen type IIA; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); collagen, type II, alpha 1 L homeolog; COLLII; cyanogen bromide; Del1; disproportionate micromelia; Dmm; Lpk; M100856; MGC131516; pro alpha1 type II collagen; pro-alpha 1(II); pro-alpha-1 type II collagen; procollagen alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; Rgsc856; SEDC; STL1; type II collagen; type II collagen cyanogen bromide fragment CB10; type II collogen cyanogen bromide fragment CB8; unnamed protein product; X-col II; xcol2a1; XELAEV_18013523mg

more info or order :

Invitrogen product webpage