HDJ2 Monoclonal Antibody (KA2A5.6) | Invitrogen MA5-12748 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HDJ2 Monoclonal Antibody (KA2A5.6)

catalog :

MA5-12748

quantity :

500 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KA2A5.6

reactivity :

Chinese hamsters, human, mouse, rat, pigs , domestic rabbit, rhesus macaque

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 3b	Jeong A, Cheng S, Zhong R, Bennett D, Bergo M, Li L. Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice. Acta Neuropathol Commun. 2021;9:129 pubmed publisher
western blot; human; loading ...; fig 6??	Sluder I, Nitika -, Knighton L, Truman A. The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity. PLoS Genet. 2018;14:e1007462 pubmed publisher
western blot; mouse; 1:1000; fig s6	Kyathanahalli C, Organ K, Moreci R, Anamthathmakula P, Hassan S, Caritis S, et al. Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. Proc Natl Acad Sci U S A. 2015;112:14090-5 pubmed publisher
western blot; human	Kabbaj F, Lu S, Faouzi M, Meddah B, Proksch P, Cherrah Y, et al. Bioactive metabolites from Chaetomium aureum: structure elucidation and inhibition of the Hsp90 machine chaperoning activity. Bioorg Med Chem. 2015;23:126-31 pubmed publisher
western blot; human	Keating G, Reid H, Eivers S, Mulvaney E, Kinsella B. Transcriptional regulation of the human thromboxane A2 receptor gene by Wilms' tumor (WT)1 and hypermethylated in cancer (HIC) 1 in prostate and breast cancers. Biochim Biophys Acta. 2014;1839:476-92 pubmed publisher
immunohistochemistry; rhesus macaque	Shimizu J, Fukuda T, Abe T, Ogihara M, Kubota J, Sasaki A, et al. Ultrasound safety with midfrequency transcranial sonothrombolysis: preliminary study on normal macaca monkey brain. Ultrasound Med Biol. 2012;38:1040-50 pubmed publisher
western blot; mouse; 1:400	Yang S, Chang S, Tu Y, Lawson G, Bergo M, Fong L, et al. Severe hepatocellular disease in mice lacking one or both CaaX prenyltransferases. J Lipid Res. 2012;53:77-86 pubmed publisher
western blot; human	Gannon A, Turner E, Reid H, Kinsella B. Regulated expression of the alpha isoform of the human thromboxane A2 receptor during megakaryocyte differentiation: a coordinated role for WT1, Egr1, and Sp1. J Mol Biol. 2009;394:29-45 pubmed publisher
western blot; human; 1:400	Coffinier C, Hudon S, Lee R, Farber E, Nobumori C, Miner J, et al. A potent HIV protease inhibitor, darunavir, does not inhibit ZMPSTE24 or lead to an accumulation of farnesyl-prelamin A in cells. J Biol Chem. 2008;283:9797-804 pubmed publisher
western blot; mouse	Coffinier C, Hudon S, Farber E, Chang S, Hrycyna C, Young S, et al. HIV protease inhibitors block the zinc metalloproteinase ZMPSTE24 and lead to an accumulation of prelamin A in cells. Proc Natl Acad Sci U S A. 2007;104:13432-7 pubmed
western blot; mouse	Shachaf C, Perez O, Youssef S, Fan A, Elchuri S, Goldstein M, et al. Inhibition of HMGcoA reductase by atorvastatin prevents and reverses MYC-induced lymphomagenesis. Blood. 2007;110:2674-84 pubmed
western blot; human	Qiu Y, Liu X, Zou W, Yue P, Lonial S, Khuri F, et al. The farnesyltransferase inhibitor R115777 up-regulates the expression of death receptor 5 and enhances TRAIL-induced apoptosis in human lung cancer cells. Cancer Res. 2007;67:4973-80 pubmed
western blot; human	Sun S, Liu X, Zou W, Yue P, Marcus A, Khuri F. The farnesyltransferase inhibitor lonafarnib induces CCAAT/enhancer-binding protein homologous protein-dependent expression of death receptor 5, leading to induction of apoptosis in human cancer cells. J Biol Chem. 2007;282:18800-9 pubmed
western blot; mouse	Uchiyama Y, Takeda N, Mori M, Terada K. Heat shock protein 40/DjB1 is required for thermotolerance in early phase. J Biochem. 2006;140:805-12 pubmed
western blot; mouse; fig 3	Nagaoka U, Kim K, Jana N, Doi H, Maruyama M, Mitsui K, et al. Increased expression of p62 in expanded polyglutamine-expressing cells and its association with polyglutamine inclusions. J Neurochem. 2004;91:57-68 pubmed
	Luttman J, Hoj J, Lin K, Lin J, Gu J, Rouse C, et al. ABL allosteric inhibitors synergize with statins to enhance apoptosis of metastatic lung cancer cells. Cell Rep. 2021;37:109880 pubmed publisher
	Su W, Chapman N, Wei J, Zeng H, Dhungana Y, Shi H, et al. Protein Prenylation Drives Discrete Signaling Programs for the Differentiation and Maintenance of Effector Treg Cells. Cell Metab. 2020;32:996-1011.e7 pubmed publisher
	Nitika -, Blackman J, Knighton L, Takakuwa J, Calderwood S, Truman A. Chemogenomic screening identifies the Hsp70 co-chaperone DNAJA1 as a hub for anticancer drug resistance. Sci Rep. 2020;10:13831 pubmed publisher
	Mijimolle N, Velasco J, Dubus P, Guerra C, Weinbaum C, Casey P, et al. Protein farnesyltransferase in embryogenesis, adult homeostasis, and tumor development. Cancer Cell. 2005;7:313-24 pubmed
	Berciano M, Villagra N, Ojeda J, Navascues J, Gomes A, Lafarga M, et al. Oculopharyngeal muscular dystrophy-like nuclear inclusions are present in normal magnocellular neurosecretory neurons of the hypothalamus. Hum Mol Genet. 2004;13:829-38 pubmed
	Delmas C, End D, Rochaix P, Favre G, Toulas C, Cohen Jonathan E. The farnesyltransferase inhibitor R115777 reduces hypoxia and matrix metalloproteinase 2 expression in human glioma xenograft. Clin Cancer Res. 2003;9:6062-8 pubmed
	Patnaik A, Eckhardt S, Izbicka E, Tolcher A, Hammond L, Takimoto C, et al. A phase I, pharmacokinetic, and biological study of the farnesyltransferase inhibitor tipifarnib in combination with gemcitabine in patients with advanced malignancies. Clin Cancer Res. 2003;9:4761-71 pubmed
	Qiao Y, Ouyang Y, Giffard R. Overexpression of HDJ-2 protects astrocytes from ischemia-like injury and reduces redistribution of ubiquitin staining in vitro. J Cereb Blood Flow Metab. 2003;23:1113-6 pubmed
	Cohen S, Ho L, Ranganathan S, Abbruzzese J, Alpaugh R, Beard M, et al. Phase II and pharmacodynamic study of the farnesyltransferase inhibitor R115777 as initial therapy in patients with metastatic pancreatic adenocarcinoma. J Clin Oncol. 2003;21:1301-6 pubmed
	Planey S, Abrams M, Robertson N, Litwack G. Role of apical caspases and glucocorticoid-regulated genes in glucocorticoid-induced apoptosis of pre-B leukemic cells. Cancer Res. 2003;63:172-8 pubmed
	Lin B, Makhov A, Griffith J, Broker T, Chow L. Chaperone proteins abrogate inhibition of the human papillomavirus (HPV) E1 replicative helicase by the HPV E2 protein. Mol Cell Biol. 2002;22:6592-604 pubmed
	Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama K, et al. CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity. Mol Cell. 2002;10:55-67 pubmed
	Britten C, Rowinsky E, Soignet S, Patnaik A, Yao S, Deutsch P, et al. A phase I and pharmacological study of the farnesyl protein transferase inhibitor L-778,123 in patients with solid malignancies. Clin Cancer Res. 2001;7:3894-903 pubmed
	Lobell R, Omer C, Abrams M, Bhimnathwala H, Brucker M, Buser C, et al. Evaluation of farnesyl:protein transferase and geranylgeranyl:protein transferase inhibitor combinations in preclinical models. Cancer Res. 2001;61:8758-68 pubmed
	Wyttenbach A, Swartz J, Kita H, Thykjaer T, Carmichael J, Bradley J, et al. Polyglutamine expansions cause decreased CRE-mediated transcription and early gene expression changes prior to cell death in an inducible cell model of Huntington's disease. Hum Mol Genet. 2001;10:1829-45 pubmed
	Peters D, Hoover R, Gerlach M, Koh E, Zhang H, Choe K, et al. Activity of the farnesyl protein transferase inhibitor SCH66336 against BCR/ABL-induced murine leukemia and primary cells from patients with chronic myeloid leukemia. Blood. 2001;97:1404-12 pubmed

product information

Product Type :

Antibody

Product Name :

HDJ2 Monoclonal Antibody (KA2A5.6)

Catalog # :

MA5-12748

Quantity :

500 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bacteria, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL

Species :

Bacteria, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat

Clone :

KA2A5.6

Isotype :

IgG1

Storage :

4° C

Description :

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1. 6 kb and a protein product with a molecular weight of about 35 kDa.

Immunogen :

N-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL

Aliases :

DJ-2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ heat shock protein family (Hsp40) member A1; dnaJ homolog subfamily A member 1; DnaJ protein homolog 2; Dnaj2; DNAJA1; dnaJ-like protein 1; DnaJ-like protein 2; DnaJ-like subfamily A member 1; h HSJ-2; HDJ2; hDj-2; Heat shock 40 kDa protein 4; heat shock protein J2; heat shock protein, DNAJ-like 2; HSDJ; HSJ2; HSJ-2; HSPF4; human DnaJ protein 2; I79_019991; LOC100346508; LOW QUALITY PROTEIN: dnaJ homolog subfamily A member 1; NEDD7; neural precursor cell expressed, developmentally down-regulated 7; neural precursor cell expressed, developmentally down-regulated gene 7; Rdj1