TGM2 Monoclonal Antibody (CUB 7402) | Invitrogen MA5-12739 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TGM2 Monoclonal Antibody (CUB 7402)

catalog :

MA5-12739

quantity :

500 µL

price :

US 404.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CUB 7402

reactivity :

guinea pig, hamsters, African green monkey, human, mouse, rat, dogs, pigs , domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, reverse phase protein lysate microarray, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 129

Published Application/Species/Sample/Dilution	Reference
reverse phase protein lysate microarray; human; loading ...; fig 7a	Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
immunocytochemistry; human western blot; human; 1:5000	CsomÃ³s K, KristÃ³f E, Jakob B, CsomÃ³s I, KovÃ¡cs G, Rotem O, et al. Protein cross-linking by chlorinated polyamines and transglutamylation stabilizes neutrophil extracellular traps. Cell Death Dis. 2016;7:e2332 pubmed publisher
immunoprecipitation; human; fig 3 western blot; human; fig 2	Altuntas S, Rossin F, Marsella C, D Eletto M, Diaz Hidalgo L, Farrace M, et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget. 2015;6:44941-54 pubmed publisher
western blot; mouse; fig 6	Myneni V, Melino G, Kaartinen M. Transglutaminase 2--a novel inhibitor of adipogenesis. Cell Death Dis. 2015;6:e1868 pubmed publisher
immunohistochemistry - frozen section; human; 1:1000; fig 1f	Di Niro R, Snir O, Kaukinen K, Yaari G, Lundin K, Gupta N, et al. Responsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease. Mucosal Immunol. 2016;9:254-64 pubmed publisher
immunocytochemistry; human	Piacentini M, D Eletto M, Farrace M, Rodolfo C, Del Nonno F, Ippolito G, et al. Characterization of distinct sub-cellular location of transglutaminase type II: changes in intracellular distribution in physiological and pathological states. Cell Tissue Res. 2014;358:793-805 pubmed publisher
immunohistochemistry; human; 1:50-100 western blot; human; 1:5000	Leicht D, Kausar T, Wang Z, Ferrer Torres D, Wang T, Thomas D, et al. TGM2: a cell surface marker in esophageal adenocarcinomas. J Thorac Oncol. 2014;9:872-81 pubmed publisher
immunohistochemistry; human	Quaglia S, De Leo L, Ziberna F, Vatta S, Villanacci V, Granzotto M, et al. Intestinal-mucosa anti-transglutaminase antibody assays to test for genetic gluten intolerance. Cell Mol Immunol. 2014;11:617-20 pubmed publisher
immunohistochemistry; rat; 5 ug/ml	Steppan J, Sikka G, Jandu S, Barodka V, Halushka M, Flavahan N, et al. Exercise, vascular stiffness, and tissue transglutaminase. J Am Heart Assoc. 2014;3:e000599 pubmed publisher
immunocytochemistry; human western blot; human	Boisvert H, Lorand L, Duncan M. Transglutaminase 2 is essential for adherence of Porphyromonas gingivalis to host cells. Proc Natl Acad Sci U S A. 2014;111:5355-60 pubmed publisher
immunocytochemistry; human western blot; human	Ku B, Lee C, Lee S, Kim S. Increased expression of transglutaminase 2 drives glycolytic metabolism in renal carcinoma cells. Amino Acids. 2014;46:1527-36 pubmed publisher
immunocytochemistry; mouse western blot; mouse; 1:500	Kim J, Hong J, Jeong E, Lee W, Kim H, Kang J, et al. Lack of transglutaminase 2 diminished T-cell responses in mice. Immunology. 2014;142:506-16 pubmed publisher
western blot; human	Lee D, Chang G. Methylglyoxal in cells elicits a negative feedback loop entailing transglutaminase 2 and glyoxalase 1. Redox Biol. 2014;2:196-205 pubmed publisher
immunohistochemistry; human; 1:200	Caruso R, Marafini I, Del Vecchio Blanco G, Fina D, Paoluzi O, Colantoni A, et al. Sampling of proximal and distal duodenal biopsies in the diagnosis and monitoring of celiac disease. Dig Liver Dis. 2014;46:323-9 pubmed publisher
immunohistochemistry; human western blot; human	Ciacci C, Russo I, Bucci C, Iovino P, Pellegrini L, Giangrieco I, et al. The kiwi fruit peptide kissper displays anti-inflammatory and anti-oxidant effects in in-vitro and ex-vivo human intestinal models. Clin Exp Immunol. 2014;175:476-84 pubmed publisher
ELISA; human; 1:200	Antonella Nadalutti C, Korponay Szabo I, Kaukinen K, Wang Z, Griffin M, Maki M, et al. Thioredoxin is involved in endothelial cell extracellular transglutaminase 2 activation mediated by celiac disease patient IgA. PLoS ONE. 2013;8:e77277 pubmed publisher
blocking or activating experiments; human; 0.5 ug/ml western blot; human	Wang Z, Perez M, Caja S, Melino G, Johnson T, Lindfors K, et al. A novel extracellular role for tissue transglutaminase in matrix-bound VEGF-mediated angiogenesis. Cell Death Dis. 2013;4:e808 pubmed publisher
ELISA; human; 1:200 western blot; human	Nadalutti C, Korponay Szabo I, Kaukinen K, Griffin M, Maki M, Lindfors K. Celiac disease patient IgA antibodies induce endothelial adhesion and cell polarization defects via extracellular transglutaminase 2. Cell Mol Life Sci. 2014;71:1315-26 pubmed publisher
ELISA; human western blot; human; 1:2000	Kanchan K, Ergülen E, Kiraly R, Simon Vecsei Z, Fuxreiter M, Fesus L. Identification of a specific one amino acid change in recombinant human transglutaminase 2 that regulates its activity and calcium sensitivity. Biochem J. 2013;455:261-72 pubmed publisher
immunoprecipitation; human; 1:1000 western blot; human; 1:1000	Currò M, Ferlazzo N, Risitano R, Condello S, Vecchio M, Caccamo D, et al. Transglutaminase 2 and phospholipase A? interactions in the inflammatory response in human Thp-1 monocytes. Amino Acids. 2014;46:759-66 pubmed publisher
immunoprecipitation; human western blot; human	Ku B, Kim D, Kim K, Yoo B, Kim S, Gong Y, et al. Transglutaminase 2 inhibition found to induce p53 mediated apoptosis in renal cell carcinoma. FASEB J. 2013;27:3487-95 pubmed publisher
flow cytometry; human ELISA; human	Iversen R, Di Niro R, Stamnaes J, Lundin K, Wilson P, Sollid L. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol. 2013;190:5981-91 pubmed publisher
immunohistochemistry; human	Bucci C, Zingone F, Russo I, Morra I, Tortora R, Pogna N, et al. Gliadin does not induce mucosal inflammation or basophil activation in patients with nonceliac gluten sensitivity. Clin Gastroenterol Hepatol. 2013;11:1294-1299.e1 pubmed publisher
western blot; rat; fig 5	Wang Z, Griffin M. The role of TG2 in regulating S100A4-mediated mammary tumour cell migration. PLoS ONE. 2013;8:e57017 pubmed publisher
western blot; human; 1:1000	Byrne G, Freeley M, Feighery C, Whelan A, Long A. Protein kinase C delta is a substrate of tissue transglutaminase and a novel autoantigen in coeliac disease. Clin Immunol. 2013;147:1-8 pubmed publisher
western blot; human	Park M, You H, Lee H, Kang J, Oh S, Kim S, et al. Transglutaminase-2 induces N-cadherin expression in TGF-?1-induced epithelial mesenchymal transition via c-Jun-N-terminal kinase activation by protein phosphatase 2A down-regulation. Eur J Cancer. 2013;49:1692-705 pubmed publisher
western blot; mouse	Yoo H, Ahn E, Kim S, Lee S, Oh S, Kim S. Divergent results induced by different types of septic shock in transglutaminase 2 knockout mice. Amino Acids. 2013;44:189-97 pubmed publisher
immunohistochemistry; human	Elli L, Roncoroni L, Hils M, Pasternack R, Barisani D, Terrani C, et al. Immunological effects of transglutaminase-treated gluten in coeliac disease. Hum Immunol. 2012;73:992-7 pubmed publisher
western blot; rat	D Argenio G, Mazzone G, Ribecco M, Lembo V, Vitaglione P, Guarino M, et al. Garlic extract attenuating rat liver fibrosis by inhibiting TGF-?1. Clin Nutr. 2013;32:252-8 pubmed publisher
immunocytochemistry; rat	Lauzier A, Charbonneau M, Paquette M, Harper K, Dubois C. Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis. Arthritis Res Ther. 2012;14:R159 pubmed publisher
western blot; human	Ai L, Skehan R, Saydi J, Lin T, Brown K. Ataxia-Telangiectasia, Mutated (ATM)/Nuclear Factor ? light chain enhancer of activated B cells (NF?B) signaling controls basal and DNA damage-induced transglutaminase 2 expression. J Biol Chem. 2012;287:18330-41 pubmed publisher
immunocytochemistry; human	Russo L, Marsella C, Nardo G, Massignan T, Alessio M, Piermarini E, et al. Transglutaminase 2 transamidation activity during first-phase insulin secretion: natural substrates in INS-1E. Acta Diabetol. 2013;50:61-72 pubmed publisher
western blot; rat; 1:2000	D Argenio G, Mazzone G, Tuccillo C, Ribecco M, Graziani G, Gravina A, et al. Apple polyphenols extract (APE) improves colon damage in a rat model of colitis. Dig Liver Dis. 2012;44:555-62 pubmed publisher
immunoprecipitation; human immunocytochemistry; human; 1:25 western blot; mouse	D Eletto M, Farrace M, Rossin F, Strappazzon F, Giacomo G, Cecconi F, et al. Type 2 transglutaminase is involved in the autophagy-dependent clearance of ubiquitinated proteins. Cell Death Differ. 2012;19:1228-38 pubmed publisher
immunohistochemistry; human western blot; human; 1:1000	Kojima S, Kuo T, Tatsukawa H. Regulation of transglutaminase-mediated hepatic cell death in alcoholic steatohepatitis and non-alcoholic steatohepatitis. J Gastroenterol Hepatol. 2012;27 Suppl 2:52-7 pubmed publisher
immunohistochemistry; human	Oikarinen M, Tauriainen S, Oikarinen S, Honkanen T, Collin P, Rantala I, et al. Type 1 diabetes is associated with enterovirus infection in gut mucosa. Diabetes. 2012;61:687-91 pubmed publisher
blocking or activating experiments; human; 1 mg/ml	Silano M, Vincentini O, Luciani A, Felli C, Caserta S, Esposito S, et al. Early tissue transglutaminase-mediated response underlies K562(S)-cell gliadin-dependent agglutination. Pediatr Res. 2012;71:532-8 pubmed publisher
immunocytochemistry; human immunohistochemistry; human western blot; human	Jung H, Chen Z, Wang M, Fayad L, Romaguera J, Kwak L, et al. Calcium blockers decrease the bortezomib resistance in mantle cell lymphoma via manipulation of tissue transglutaminase activities. Blood. 2012;119:2568-78 pubmed publisher
immunocytochemistry; human western blot; human	Kumar A, Xu J, Sung B, Kumar S, Yu D, Aggarwal B, et al. Evidence that GTP-binding domain but not catalytic domain of transglutaminase 2 is essential for epithelial-to-mesenchymal transition in mammary epithelial cells. Breast Cancer Res. 2012;14:R4 pubmed
immunocytochemistry; rat; 1:500 western blot; rat; 1:1000	Campisi A, Spatuzza M, Russo A, Raciti G, Vanella A, Stanzani S, et al. Expression of tissue transglutaminase on primary olfactory ensheathing cells cultures exposed to stress conditions. Neurosci Res. 2012;72:289-95 pubmed publisher
western blot; human	van Geel R, Debets M, Löwik D, Pruijn G, Boelens W. Detection of transglutaminase activity using click chemistry. Amino Acids. 2012;43:1251-63 pubmed publisher
blocking or activating experiments; human; 2 ug/ml	Caputo I, Lepretti M, Secondo A, Martucciello S, Paolella G, Sblattero D, et al. Anti-tissue transglutaminase antibodies activate intracellular tissue transglutaminase by modulating cytosolic Ca2+ homeostasis. Amino Acids. 2013;44:251-60 pubmed publisher
immunocytochemistry; guinea pig; 1:1000 immunohistochemistry; guinea pig; 1:1000	Wilhelmus M, de Jager M, Rozemuller A, Brevé J, Bol J, Eckert R, et al. Transglutaminase 1 and its regulator tazarotene-induced gene 3 localize to neuronal tau inclusions in tauopathies. J Pathol. 2012;226:132-42 pubmed publisher
immunocytochemistry; mouse; 1:500 western blot; mouse; 1:1000	van Strien M, Baron W, Bakker E, Bauer J, Bol J, Brevé J, et al. Tissue transglutaminase activity is involved in the differentiation of oligodendrocyte precursor cells into myelin-forming oligodendrocytes during CNS remyelination. Glia. 2011;59:1622-34 pubmed publisher
immunocytochemistry; human	Zemskov E, Mikhailenko I, Smith E, Belkin A. Tissue transglutaminase promotes PDGF/PDGFR-mediated signaling and responses in vascular smooth muscle cells. J Cell Physiol. 2012;227:2089-96 pubmed publisher
immunohistochemistry; human	Stenberg R, Kaukinen K, Bengtsson M, Lindberg E, Dahle C. Early developing celiac disease in children with cerebral palsy. J Pediatr Gastroenterol Nutr. 2011;53:674-8 pubmed publisher
immunohistochemistry; human	Ruuskanen A, Luostarinen L, Collin P, Krekelä I, Patrikainen H, Tillonen J, et al. Persistently positive gliadin antibodies without transglutaminase antibodies in the elderly: gluten intolerance beyond coeliac disease. Dig Liver Dis. 2011;43:772-8 pubmed publisher
flow cytometry; mouse immunocytochemistry; mouse	Zemskov E, Mikhailenko I, Hsia R, Zaritskaya L, Belkin A. Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes. PLoS ONE. 2011;6:e19414 pubmed publisher
flow cytometry; human; fig 4 western blot; human; 1:200; fig 4	Chiang N, Hsiao C, Huang Y, Chen H, Hsieh I, Chang G, et al. Disease-associated GPR56 mutations cause bilateral frontoparietal polymicrogyria via multiple mechanisms. J Biol Chem. 2011;286:14215-25 pubmed publisher
western blot; human	Tatsukawa H, Sano T, Fukaya Y, Ishibashi N, Watanabe M, Okuno M, et al. Dual induction of caspase 3- and transglutaminase-dependent apoptosis by acyclic retinoid in hepatocellular carcinoma cells. Mol Cancer. 2011;10:4 pubmed publisher
immunohistochemistry; human; 1:500	Wilhelmus M, Verhaar R, Andringa G, Bol J, Cras P, Shan L, et al. Presence of tissue transglutaminase in granular endoplasmic reticulum is characteristic of melanized neurons in Parkinson's disease brain. Brain Pathol. 2011;21:130-9 pubmed publisher
immunocytochemistry; human western blot; human	Kim D, Han B, Park K, Lee B, Kim S, Bae C. I-kappaBalpha depletion by transglutaminase 2 and mu-calpain occurs in parallel with the ubiquitin-proteasome pathway. Biochem Biophys Res Commun. 2010;399:300-6 pubmed publisher
immunohistochemistry; African green monkey; 1:2000	Steele K, Anderson A, Mohamadzadeh M. Fibroblastic reticular cell infection by hemorrhagic fever viruses. Immunotherapy. 2009;1:187-97 pubmed publisher
western blot; human	Frese Schaper M, Schardt J, Sakai T, Carboni G, Schmid R, Frese S. Inhibition of tissue transglutaminase sensitizes TRAIL-resistant lung cancer cells through upregulation of death receptor 5. FEBS Lett. 2010;584:2867-71 pubmed publisher
immunohistochemistry; human	Koskinen O, Lindfors K, Collin P, Peräaho M, Laurila K, Woolley N, et al. Intestinal transglutaminase 2 specific antibody deposits in non-responsive coeliac disease. Dig Liver Dis. 2010;42:692-7 pubmed publisher
western blot; human; 1:1000 immunohistochemistry; rat; 1:1000	Boscolo S, Lorenzon A, Sblattero D, Florian F, Stebel M, Marzari R, et al. Anti transglutaminase antibodies cause ataxia in mice. PLoS ONE. 2010;5:e9698 pubmed publisher
immunocytochemistry; dogs	Prunotto M, Compagnone A, Bruschi M, Candiano G, Colombatto S, Bandino A, et al. Endocellular polyamine availability modulates epithelial-to-mesenchymal transition and unfolded protein response in MDCK cells. Lab Invest. 2010;90:929-39 pubmed publisher
immunocytochemistry; human ELISA; human; 1:1000 ELISA; guinea pig; 1:1000	Faye C, Inforzato A, Bignon M, Hartmann D, Muller L, Ballut L, et al. Transglutaminase-2: a new endostatin partner in the extracellular matrix of endothelial cells. Biochem J. 2010;427:467-75 pubmed publisher
immunoprecipitation; human western blot; human; 1:100	Luciani A, Villella V, Vasaturo A, Giardino I, Pettoello Mantovani M, Guido S, et al. Lysosomal accumulation of gliadin p31-43 peptide induces oxidative stress and tissue transglutaminase-mediated PPARgamma downregulation in intestinal epithelial cells and coeliac mucosa. Gut. 2010;59:311-9 pubmed publisher
western blot; human	Suenaga Y, Kaneko Y, Matsumoto D, Hossain M, Ozaki T, Nakagawara A. Positive auto-regulation of MYCN in human neuroblastoma. Biochem Biophys Res Commun. 2009;390:21-6 pubmed publisher
immunohistochemistry; human; 1:100	Gorgun J, Portyanko A, Marakhouski Y, Cherstvoy E. Tissue transglutaminase expression in celiac mucosa: an immunohistochemical study. Virchows Arch. 2009;455:363-73 pubmed publisher
flow cytometry; human immunoprecipitation; human	Telci D, Collighan R, Basaga H, Griffin M. Increased TG2 expression can result in induction of transforming growth factor beta1, causing increased synthesis and deposition of matrix proteins, which can be regulated by nitric oxide. J Biol Chem. 2009;284:29547-58 pubmed publisher
immunocytochemistry; human; 1:30	Malorni W, Farrace M, Matarrese P, Tinari A, Ciarlo L, Mousavi Shafaei P, et al. The adenine nucleotide translocator 1 acts as a type 2 transglutaminase substrate: implications for mitochondrial-dependent apoptosis. Cell Death Differ. 2009;16:1480-92 pubmed publisher
immunoprecipitation; human western blot; human; 1:500	Luciani A, Villella V, Vasaturo A, Giardino I, Raia V, Pettoello Mantovani M, et al. SUMOylation of tissue transglutaminase as link between oxidative stress and inflammation. J Immunol. 2009;183:2775-84 pubmed publisher
immunohistochemistry; pigs	Huebner J, Johnson K, Kraus V, Terkeltaub R. Transglutaminase 2 is a marker of chondrocyte hypertrophy and osteoarthritis severity in the Hartley guinea pig model of knee OA. Osteoarthritis Cartilage. 2009;17:1056-64 pubmed publisher
western blot; mouse; 1:500 western blot; human; 1:500	Kwon M, Jung J, Jung S, Park J, Kim Y, Ha K. Quantitative and rapid analysis of transglutaminase activity using protein arrays in mammalian cells. Mol Cells. 2009;27:337-43 pubmed publisher
blocking or activating experiments; human; 1:400 immunohistochemistry; human; 1:100	Anjum N, Baker P, Robinson N, Aplin J. Maternal celiac disease autoantibodies bind directly to syncytiotrophoblast and inhibit placental tissue transglutaminase activity. Reprod Biol Endocrinol. 2009;7:16 pubmed publisher
immunohistochemistry; human; 1:200 western blot; human	Cho B, Kim M, Suh D, Hahn J, Lee B, Choi Y, et al. Increased tissue transglutaminase expression in human atherosclerotic coronary arteries. Coron Artery Dis. 2008;19:459-68 pubmed publisher
western blot; rat; 1:1000	Bramanti V, Campisi A, Tomassoni D, Li Volti G, Caccamo D, Cannavo G, et al. Effect of acetylcholine precursors on proliferation and differentiation of astroglial cells in primary cultures. Neurochem Res. 2008;33:2601-8 pubmed publisher
immunohistochemistry; human; 6.5 ug/ml	Hadjivassiliou M, Aeschlimann P, Strigun A, Sanders D, Woodroofe N, Aeschlimann D. Autoantibodies in gluten ataxia recognize a novel neuronal transglutaminase. Ann Neurol. 2008;64:332-43 pubmed publisher
western blot; human	Kim D, Park K, Jeong K, Lee B, Lee C, Kim S. Glucosamine is an effective chemo-sensitizer via transglutaminase 2 inhibition. Cancer Lett. 2009;273:243-9 pubmed publisher
western blot; human	Lai T, Liu Y, Tucker T, Daniel K, Sane D, Toone E, et al. Identification of chemical inhibitors to human tissue transglutaminase by screening existing drug libraries. Chem Biol. 2008;15:969-78 pubmed publisher
ELISA; human western blot; human	Stamnaes J, Fleckenstein B, Lund Johansen F, Sollid L. The monoclonal antibody 6B9 recognizes CD44 and not cell surface transglutaminase 2. Scand J Immunol. 2008;68:534-42 pubmed publisher
immunohistochemistry; human; 1:500	Wilhelmus M, Grunberg S, Bol J, van Dam A, Hoozemans J, Rozemuller A, et al. Transglutaminases and transglutaminase-catalyzed cross-links colocalize with the pathological lesions in Alzheimer's disease brain. Brain Pathol. 2009;19:612-22 pubmed publisher
western blot; mouse	Telci D, Wang Z, Li X, Verderio E, Humphries M, Baccarini M, et al. Fibronectin-tissue transglutaminase matrix rescues RGD-impaired cell adhesion through syndecan-4 and beta1 integrin co-signaling. J Biol Chem. 2008;283:20937-47 pubmed publisher
western blot; human	Li H, Shen J, Chang S, Hsu W, Sung Y. Calcitonin promotes outgrowth of trophoblast cells on endometrial epithelial cells: involvement of calcium mobilization and protein kinase C activation. Placenta. 2008;29:20-9 pubmed
western blot; hamsters	Zemskov E, Mikhailenko I, Strickland D, Belkin A. Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1. J Cell Sci. 2007;120:3188-99 pubmed
blocking or activating experiments; human; 2.5 ug/ml	Barone M, Caputo I, Ribecco M, Maglio M, Marzari R, Sblattero D, et al. Humoral immune response to tissue transglutaminase is related to epithelial cell proliferation in celiac disease. Gastroenterology. 2007;132:1245-53 pubmed
western blot; human	Kim D, Park S, Nam B, Kim I, Kim S. Reversal of drug resistance in breast cancer cells by transglutaminase 2 inhibition and nuclear factor-kappaB inactivation. Cancer Res. 2006;66:10936-43 pubmed
western blot; human	Zanoni G, Navone R, Lunardi C, Tridente G, Bason C, Sivori S, et al. In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes. PLoS Med. 2006;3:e358 pubmed
ELISA; human; 1:1000 western blot; human; 1:1000	Byrne G, Ryan F, Jackson J, Feighery C, Kelly J. Mutagenesis of the catalytic triad of tissue transglutaminase abrogates coeliac disease serum IgA autoantibody binding. Gut. 2007;56:336-41 pubmed
immunohistochemistry; human; 1:500 western blot; human; 1:500	Singer C, Hudelist G, Walter I, Rueckliniger E, Czerwenka K, Kubista E, et al. Tissue array-based expression of transglutaminase-2 in human breast and ovarian cancer. Clin Exp Metastasis. 2006;23:33-9 pubmed
immunocytochemistry; human western blot; human; 1:2000 or 1:8000	Balajthy Z, Csomós K, Vámosi G, Szanto A, Lanotte M, Fesus L. Tissue-transglutaminase contributes to neutrophil granulocyte differentiation and functions. Blood. 2006;108:2045-54 pubmed
western blot; mouse	Strnad P, Siegel M, Toivola D, Choi K, Kosek J, Khosla C, et al. Pharmacologic transglutaminase inhibition attenuates drug-primed liver hypertrophy but not Mallory body formation. FEBS Lett. 2006;580:2351--2357 pubmed
immunocytochemistry; mouse western blot; mouse	Al Jallad H, Nakano Y, Chen J, McMillan E, Lefebvre C, Kaartinen M. Transglutaminase activity regulates osteoblast differentiation and matrix mineralization in MC3T3-E1 osteoblast cultures. Matrix Biol. 2006;25:135-48 pubmed
western blot; rat	Caccamo D, Campisi A, Currò M, Aguennouz M, Li Volti G, Avola R, et al. Nuclear factor-kappab activation is associated with glutamate-evoked tissue transglutaminase up-regulation in primary astrocyte cultures. J Neurosci Res. 2005;82:858-65 pubmed
immunohistochemistry; mouse; 1:100 immunohistochemistry; human; 1:100 western blot; human; 1:2000	Chlenski A, Liu S, Guerrero L, Yang Q, Tian Y, Salwen H, et al. SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix. Int J Cancer. 2006;118:310-6 pubmed
immunoprecipitation; mouse	Chae K, Kim J, Park W, Kim Y, Yun H, Kwon N, et al. Distinct pH modulation for dual function of Galphah (transglutaminase II). J Biochem. 2005;137:407-13 pubmed
immunocytochemistry; human immunohistochemistry; human western blot; human immunocytochemistry; guinea pig immunohistochemistry; guinea pig western blot; guinea pig	Mehta K. Mammalian transglutaminases: a family portrait. Prog Exp Tumor Res. 2005;38:1-18 pubmed
ELISA; mouse; 1:5000 ELISA; human; 1:5000	Di Niro R, Ferrara F, Not T, Bradbury A, Chirdo F, Marzari R, et al. Characterizing monoclonal antibody epitopes by filtered gene fragment phage display. Biochem J. 2005;388:889-94 pubmed
immunoprecipitation; human; fig 4 western blot; human; fig 4	Rodolfo C, Mormone E, Matarrese P, Ciccosanti F, Farrace M, Garofano E, et al. Tissue transglutaminase is a multifunctional BH3-only protein. J Biol Chem. 2004;279:54783-92 pubmed
western blot; rat; 1:1000	Wu Y, Lai W, Tsai Y. Characterization of purified rat testicular transglutaminase and age-dependent changes of the enzyme activities. Int J Biochem Cell Biol. 2005;37:386-96 pubmed
	Ulukan B, Bihorac A, Sipahioglu T, Kiraly R, Fesus L, Telci D. Role of Tissue Transglutaminase Catalytic and Guanosine Triphosphate-Binding Domains in Renal Cell Carcinoma Progression. ACS Omega. 2020;5:28273-28284 pubmed publisher
	Lee S, Kang J, Ha J, Lee J, Oh S, Choi H, et al. Transglutaminase 2-Mediated p53 Depletion Promotes Angiogenesis by Increasing HIF-1α-p300 Binding in Renal Cell Carcinoma. Int J Mol Sci. 2020;21: pubmed publisher
	Cho S, Oh Y, Jeong E, Park S, Lee D, Wang X, et al. Amplification of transglutaminase 2 enhances tumor-promoting inflammation in gastric cancers. Exp Mol Med. 2020;52:854-864 pubmed publisher
	Trus I, Bérubé N, Jiang P, Rak J, Gerdts V, Karniychuk U. Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells. Viruses. 2020;12: pubmed publisher
	Villella V, Venerando A, Cozza G, Esposito S, Ferrari E, Monzani R, et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J. 2019;38: pubmed publisher
	Hietikko M, Koskinen O, Kurppa K, Laurila K, Saavalainen P, Salmi T, et al. Small-intestinal TG2-specific plasma cells at different stages of coeliac disease. BMC Immunol. 2018;19:36 pubmed publisher
	Lee S, Lee W, Kim N, Kang J, Kim K, Kim S, et al. Renal Cell Carcinoma Is Abrogated by p53 Stabilization through Transglutaminase 2 Inhibition. Cancers (Basel). 2018;10: pubmed publisher
	Costa S, Currò G, Pellegrino S, Lucanto M, Tuccari G, Ieni A, et al. Case report on pathogenetic link between gluten and IgA nephropathy. BMC Gastroenterol. 2018;18:64 pubmed publisher
	Condello S, Sima L, Ivan C, Cárdenas H, Schiltz G, Mishra R, et al. Tissue Tranglutaminase Regulates Interactions between Ovarian Cancer Stem Cells and the Tumor Niche. Cancer Res. 2018;78:2990-3001 pubmed publisher
	Piacentini M, Baiocchini A, Del Nonno F, Melino G, Barlev N, Rossin F, et al. Non-alcoholic fatty liver disease severity is modulated by transglutaminase type 2. Cell Death Dis. 2018;9:257 pubmed publisher
	Borrelli M, Maglio M, Korponay Szabo I, Vass V, Mearin M, Meijer C, et al. Intestinal anti-transglutaminase2 IgA deposits in children at risk for coeliac disease: data from the Prevent CD study. Clin Exp Immunol. 2017;: pubmed publisher
	Del Duca S, Serafini Fracassini D, Bonner P, Cresti M, Cai G. Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubules and actin filaments. Biochem J. 2009;418:651-64 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

TGM2 Monoclonal Antibody (CUB 7402)

Catalog # :

MA5-12739

Quantity :

500 µL

Price :

US 404.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Canine, Guinea Pig, Human, Rabbit

Applications :

Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Canine, Guinea Pig, Human, Rabbit

Clone :

CUB 7402

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Transglutaminase (TGase) catalyses the crosslink of proteins by forming e-(g-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. In mammals, eight distinct TGase isoenzymes have been identified. Tissue transglutaminase (tTGase), also known as TGase 2, has four distinct domains: N-terminal b-sandwich, catalytic core and two C-terminal b-barrel domains. tTGase may have a role in cell death, cell proliferation, cell differentiation, and receptor-mediated endocytosis. In the Alzheimer's disease brain, the elevated tTGase activity is manifested by polymerization of a number of proteins, including Ab peptide, b-amyloid precursor protein and the tau protein, with formation of neurofibrillary tangles.

Immunogen :

Purified guinea pig liver TGase II

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

C polypeptide; C polypeptide, protein-glutamine-gamma-glutamyltransferase; G alpha h; G[a]h; G-ALPHA-h; GNAH; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; RP5-1054A22.2; TG C; TG(C); TG2; TGase C; TGase H; TGase2; TGase-2; TGase-H; TgaseII; TGC; TGM2; Tissue transglutaminase; Tissue type transglutaminase; tissue-type transglutaminase; transglutaminase; transglutaminase 2; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2 C polypeptide; transglutaminase 2, C polypeptide; transglutaminase C; Transglutaminase H; transglutaminase-2; tTG; tTGas

more info or order :

Invitrogen product webpage