product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Cyclin D3 Monoclonal Antibody (DCS-22)
catalog :
MA5-12717
quantity :
500 µL
price :
US 447.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DCS-22
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3a
Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  • western blot; human
Basile K, Le K, Hartsough E, Aplin A. Inhibition of mutant BRAF splice variant signaling by next-generation, selective RAF inhibitors. Pigment Cell Melanoma Res. 2014;27:479-84 pubmed publisher
  • western blot; human
Bisteau X, Paternot S, Colleoni B, Ecker K, Coulonval K, De Groote P, et al. CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point. PLoS Genet. 2013;9:e1003546 pubmed publisher
  • immunohistochemistry; human
Canzonieri V, Barzan L, Franchin G, Vaccher E, Talamini R, Sulfaro S, et al. Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas. J Cell Physiol. 2012;227:233-8 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
  • immunoprecipitation; human
  • western blot; human
Mori M, Kitazume M, Ose R, Kurokawa J, Koga K, Osuga Y, et al. Death effector domain-containing protein (DEDD) is required for uterine decidualization during early pregnancy in mice. J Clin Invest. 2011;121:318-27 pubmed publisher
  • immunohistochemistry; mouse
Mirando A, Maruyama T, Fu J, Yu H, Hsu W. ?-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis. BMC Dev Biol. 2010;10:116 pubmed publisher
  • immunoprecipitation; rat
  • western blot; rat
Blancquaert S, Wang L, Paternot S, Coulonval K, Dumont J, Harris T, et al. cAMP-dependent activation of mammalian target of rapamycin (mTOR) in thyroid cells. Implication in mitogenesis and activation of CDK4. Mol Endocrinol. 2010;24:1453-68 pubmed publisher
  • immunohistochemistry; human; 1:50
Sarkar R, Hunter I, Rajaganeshan R, Perry S, Guillou P, Jayne D. Expression of cyclin D2 is an independent predictor of the development of hepatic metastasis in colorectal cancer. Colorectal Dis. 2010;12:316-23 pubmed publisher
  • western blot; human
Bockstaele L, Bisteau X, Paternot S, Roger P. Differential regulation of cyclin-dependent kinase 4 (CDK4) and CDK6, evidence that CDK4 might not be activated by CDK7, and design of a CDK6 activating mutation. Mol Cell Biol. 2009;29:4188-200 pubmed publisher
  • western blot; human
Tiedemann R, Schmidt J, Keats J, Shi C, Zhu Y, Palmer S, et al. Identification of a potent natural triterpenoid inhibitor of proteosome chymotrypsin-like activity and NF-kappaB with antimyeloma activity in vitro and in vivo. Blood. 2009;113:4027-37 pubmed publisher
  • western blot; mouse
Zhang Y, Xu P, Park K, Choi Y, Moore D, Wang L. Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation. Hepatology. 2008;48:289-98 pubmed publisher
  • immunohistochemistry; human; 1:50
Filipits M, Pirker R, Dunant A, Lantuejoul S, Schmid K, Huynh A, et al. Cell cycle regulators and outcome of adjuvant cisplatin-based chemotherapy in completely resected non-small-cell lung cancer: the International Adjuvant Lung Cancer Trial Biologic Program. J Clin Oncol. 2007;25:2735-40 pubmed
  • western blot; human
Haraguchi T, Koujin T, Osakada H, Kojidani T, Mori C, Masuda H, et al. Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells. J Cell Sci. 2007;120:1967-77 pubmed
  • western blot; human
Paternot S, Dumont J, Roger P. Differential utilization of cyclin D1 and cyclin D3 in the distinct mitogenic stimulations by growth factors and TSH of human thyrocytes in primary culture. Mol Endocrinol. 2006;20:3279-92 pubmed
  • western blot; mouse
Leslie K, Lang C, Devgan G, Azare J, Berishaj M, Gerald W, et al. Cyclin D1 is transcriptionally regulated by and required for transformation by activated signal transducer and activator of transcription 3. Cancer Res. 2006;66:2544-52 pubmed
  • western blot; human
Piva R, Chiarle R, Manazza A, Taulli R, Simmons W, Ambrogio C, et al. Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas. Blood. 2006;107:689-97 pubmed
  • immunohistochemistry; human; 1:100
Fu K, Weisenburger D, Greiner T, Dave S, Wright G, Rosenwald A, et al. Cyclin D1-negative mantle cell lymphoma: a clinicopathologic study based on gene expression profiling. Blood. 2005;106:4315-21 pubmed
  • western blot; dogs
Fortemaison N, Blancquaert S, Dumont J, Maenhaut C, Aktories K, Roger P, et al. Differential involvement of the actin cytoskeleton in differentiation and mitogenesis of thyroid cells: inactivation of Rho proteins contributes to cyclic adenosine monophosphate-dependent gene expression but prevents mitogenesis. Endocrinology. 2005;146:5485-95 pubmed
  • immunohistochemistry; human; 1:50
Wong S, Chan A, Lo E, Lo Y. Nuclear beta-catenin expression is rare and its potential association with short survival in colorectal signet-ring cell carcinoma. Appl Immunohistochem Mol Morphol. 2005;13:248-51 pubmed
  • western blot; human
Mermelshtein A, Gerson A, Walfisch S, Delgado B, Shechter Maor G, Delgado J, et al. Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas. Br J Cancer. 2005;93:338-45 pubmed
  • western blot; human
Siitonen T, Koistinen P, Savolainen E. Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells. Leuk Res. 2005;29:1335-42 pubmed
  • immunohistochemistry - paraffin section; human
Gloghini A, Gaidano G, Larocca L, Pierconti F, Cingolani A, Dal Maso L, et al. Expression of cyclin-dependent kinase inhibitor p27(Kip1) in AIDS-related diffuse large-cell lymphomas is associated with Epstein-Barr virus-encoded latent membrane protein 1. Am J Pathol. 2002;161:163-71 pubmed
Paternot S, Colleoni B, Bisteau X, Roger P. The CDK4/CDK6 inhibitor PD0332991 paradoxically stabilizes activated cyclin D3-CDK4/6 complexes. Cell Cycle. 2014;13:2879-88 pubmed publisher
Bockstaele L, Kooken H, Libert F, Paternot S, Dumont J, De Launoit Y, et al. Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors". Mol Cell Biol. 2006;26:5070-85 pubmed
Jirawatnotai S, Aziyu A, Osmundson E, Moons D, Zou X, Kineman R, et al. Cdk4 is indispensable for postnatal proliferation of the anterior pituitary. J Biol Chem. 2004;279:51100-6 pubmed
Petermann A, Hiromura K, Pippin J, Blonski M, Couser W, Kopp J, et al. Differential expression of d-type cyclins in podocytes in vitro and in vivo. Am J Pathol. 2004;164:1417-24 pubmed
Faast R, White J, Cartwright P, Crocker L, Sarcevic B, Dalton S. Cdk6-cyclin D3 activity in murine ES cells is resistant to inhibition by p16(INK4a). Oncogene. 2004;23:491-502 pubmed
Piva R, Liu J, Chiarle R, Podda A, Pagano M, Inghirami G. In vivo interference with Skp1 function leads to genetic instability and neoplastic transformation. Mol Cell Biol. 2002;22:8375-87 pubmed
Filipits M, Jaeger U, Pohl G, Stranzl T, Simonitsch I, Kaider A, et al. Cyclin D3 is a predictive and prognostic factor in diffuse large B-cell lymphoma. Clin Cancer Res. 2002;8:729-33 pubmed
Tong W, Pollard J. Genetic evidence for the interactions of cyclin D1 and p27(Kip1) in mice. Mol Cell Biol. 2001;21:1319-28 pubmed
Servant M, Coulombe P, Turgeon B, Meloche S. Differential regulation of p27(Kip1) expression by mitogenic and hypertrophic factors: Involvement of transcriptional and posttranscriptional mechanisms. J Cell Biol. 2000;148:543-56 pubmed
Ladha M, Lee K, Upton T, Reed M, Ewen M. Regulation of exit from quiescence by p27 and cyclin D1-CDK4. Mol Cell Biol. 1998;18:6605-15 pubmed
Dei Tos A, Piccinin S, Doglioni C, Vukosavljevic T, Mentzel T, Boiocchi M, et al. Molecular aberrations of the G1-S checkpoint in myxoid and round cell liposarcoma. Am J Pathol. 1997;151:1531-9 pubmed
image
image 1 :
Invitrogen MA5-12717 image 1
image 2 :
Invitrogen MA5-12717 image 2
image 3 :
Invitrogen MA5-12717 image 3
Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin D3 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
product information
Product Type :
Antibody
Product Name :
Cyclin D3 Monoclonal Antibody (DCS-22)
Catalog # :
MA5-12717
Quantity :
500 µL
Price :
US 447.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Mouse, Non-human primate, Rat
Applications :
Immunohistochemistry (Paraffin): 1:25, Immunoprecipitation: 2 µg/mL, Western Blot: 1 µg/mL
Species :
Human, Mouse, Non-human primate, Rat
Clone :
DCS-22
Isotype :
IgG1, kappa
Storage :
4° C
Description :
The immunogen for the anti-h-Cyclin D3 was a recombinant full length Cyclin D3 protein recognizing a 34 kD protein. Cyclin D3 is a G1 cyclin closely related to Cyclins D1 and D2 phase progression. Like Cyclin D1 and Cyclin D2, it is activated by the same mechanisms and can phosphorylate pRB when associated with cdk4 and cdk6.
Immunogen :
Purified human recombinant full length cyclin D3 protein
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:25, Immunoprecipitation: 2 µg/mL, Western Blot: 1 µg/mL
Aliases :
9230106B05Rik; AA682053; AL024085; AW146355; C78795; CCND 3; Ccnd3; CGD3; cyclin D3; CyclinD3; Cyl-3; D3 type cyclin; D3-type cyclin; G1/S-specific cyclin-D3; OTTHUMP00000223415; RP5-973N23.3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA