Cyclin D1 Monoclonal Antibody (DCS-11) | Invitrogen MA5-12707 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin D1 Monoclonal Antibody (DCS-11)

catalog :

MA5-12707

quantity :

500 µL

price :

US 389.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-11

reactivity :

human, mouse, rat

citations: 17

Reference
Knudsen E, Kumarasamy V, Nambiar R, Pearson J, Vail P, Rosenheck H, et al. CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities. Cell Rep. 2022;38:110448 pubmed publisher
Guiley K, Stevenson J, Lou K, Barkovich K, Kumarasamy V, Wijeratne T, et al. p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition. Science. 2019;366: pubmed publisher
Bisteau X, Paternot S, Colleoni B, Ecker K, Coulonval K, De Groote P, et al. CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point. PLoS Genet. 2013;9:e1003546 pubmed publisher
Gully C, Velazquez Torres G, Shin J, Fuentes Mattei E, Wang E, Carlock C, et al. Aurora B kinase phosphorylates and instigates degradation of p53. Proc Natl Acad Sci U S A. 2012;109:E1513-22 pubmed publisher
Rocha A, Paternot S, Coulonval K, Dumont J, Soares P, Roger P. Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation. Mol Biol Cell. 2008;19:4814-25 pubmed publisher
Zhang Y, Xu P, Park K, Choi Y, Moore D, Wang L. Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation. Hepatology. 2008;48:289-98 pubmed publisher
Rubio D, Garcia S, Paz M, de la Cueva T, Lopez Fernandez L, Lloyd A, et al. Molecular characterization of spontaneous mesenchymal stem cell transformation. PLoS ONE. 2008;3:e1398 pubmed publisher
Wang C, Fan S, Li Z, Fu M, Rao M, Ma Y, et al. Cyclin D1 antagonizes BRCA1 repression of estrogen receptor alpha activity. Cancer Res. 2005;65:6557-67 pubmed
Munoz Alonso M, Acosta J, Richard C, Delgado M, Sedivy J, Leon J. p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells. J Biol Chem. 2005;280:18120-9 pubmed
Ren S, Rollins B. Cyclin C/cdk3 promotes Rb-dependent G0 exit. Cell. 2004;117:239-51 pubmed
Hutter E, Renner K, Pfister G, Stöckl P, Jansen Durr P, Gnaiger E. Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J. 2004;380:919-28 pubmed
Bearss D, Lee R, Troyer D, Pestell R, Windle J. Differential effects of p21(WAF1/CIP1) deficiency on MMTV-ras and MMTV-myc mammary tumor properties. Cancer Res. 2002;62:2077-84 pubmed
Wagner M, Hampel B, Hutter E, Pfister G, Krek W, Zwerschke W, et al. Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2. Exp Gerontol. 2001;37:41-55 pubmed
Hennigan R, Stambrook P. Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes. Mol Biol Cell. 2001;12:2352-63 pubmed
Latella L, Sacco A, Pajalunga D, Tiainen M, Macera D, D ANGELO M, et al. Reconstitution of cyclin D1-associated kinase activity drives terminally differentiated cells into the cell cycle. Mol Cell Biol. 2001;21:5631-43 pubmed
Ravanko K, Jarvinen K, Paasinen Sohns A, Hölttä E. Loss of p27Kip1 from cyclin E/cyclin-dependent kinase (CDK) 2 but not from cyclin D1/CDK4 complexes in cells transformed by polyamine biosynthetic enzymes. Cancer Res. 2000;60:5244-53 pubmed
Tong W, Pollard J. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biol. 1999;19:2251-64 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin D1 Monoclonal Antibody (DCS-11)

Catalog # :

MA5-12707

Quantity :

500 µL

Price :

US 389.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Functional Assay: Assay-dependent

Species :

Human, Mouse, Rat

Clone :

DCS-11

Isotype :

IgG2a

Storage :

4° C

Description :

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

Immunogen :

Human recombinant full length cyclin D1 protein

Format :

Liquid

Applications w/Dilutions :

Functional Assay: Assay-dependent

Aliases :

AI327039; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cD1; cDNA; CycD1; cyclin D1; Cyl-1; D11S287E; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD1; PRAD-1; PRAD1 oncogene; U21B31