Cyclin D1 Monoclonal Antibody (DCS-6), Biotin | Invitrogen MA5-12699 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin D1 Monoclonal Antibody (DCS-6), Biotin

catalog :

MA5-12699

quantity :

500µL

price :

US 375.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

DCS-6

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 105

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:400	Do S, Kim K, Lee H, Kim H, Do T, Yun J, et al. Aberrant expression pattern and location of cullin 1 are associated with the development of papillary carcinoma in thyroid and cyclin D1 expression. Endocr Pathol. 2014;25:282-7 pubmed publisher
western blot; mouse	Cerqueira A, Martin A, Symonds C, Odajima J, Dubus P, Barbacid M, et al. Genetic characterization of the role of the Cip/Kip family of proteins as cyclin-dependent kinase inhibitors and assembly factors. Mol Cell Biol. 2014;34:1452-9 pubmed publisher
immunohistochemistry; human; 1:50	Wang H, Wang H, Makki M, Wen J, Dai Y, Shi Q, et al. Overexpression of ?-catenin and cyclinD1 predicts a poor prognosis in ovarian serous carcinomas. Int J Clin Exp Pathol. 2014;7:264-71 pubmed
western blot; human	Elong Edimo W, Schurmans S, Roger P, Erneux C. SHIP2 signaling in normal and pathological situations: Its impact on cell proliferation. Adv Biol Regul. 2014;54:142-51 pubmed publisher
western blot; mouse	Shukla S, Bhaskaran N, Babcook M, Fu P, MacLennan G, Gupta S. Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway. Carcinogenesis. 2014;35:452-60 pubmed publisher
western blot; human	Du Q, Zhang X, Liu Q, Zhang X, Bartels C, Geller D. Nitric oxide production upregulates Wnt/?-catenin signaling by inhibiting Dickkopf-1. Cancer Res. 2013;73:6526-37 pubmed publisher
immunohistochemistry; human; 1:25	Wake N, Ricketts C, Morris M, Prigmore E, Gribble S, Skytte A, et al. UBE2QL1 is disrupted by a constitutional translocation associated with renal tumor predisposition and is a novel candidate renal tumor suppressor gene. Hum Mutat. 2013;34:1650-61 pubmed publisher
immunohistochemistry; rat; 1:500 western blot; rat; 1:500	Wu J, Raver C, Piao C, Keller A, Faden A. Cell cycle activation contributes to increased neuronal activity in the posterior thalamic nucleus and associated chronic hyperesthesia after rat spinal cord contusion. Neurotherapeutics. 2013;10:520-38 pubmed publisher
western blot; human	Bisteau X, Paternot S, Colleoni B, Ecker K, Coulonval K, De Groote P, et al. CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point. PLoS Genet. 2013;9:e1003546 pubmed publisher
western blot; human	Greussing R, Hackl M, Charoentong P, Pauck A, Monteforte R, Cavinato M, et al. Identification of microRNA-mRNA functional interactions in UVB-induced senescence of human diploid fibroblasts. BMC Genomics. 2013;14:224 pubmed publisher
western blot; human; 1:200	Liu L, Xu Y, Reiter R. Melatonin inhibits the proliferation of human osteosarcoma cell line MG-63. Bone. 2013;55:432-8 pubmed publisher
immunohistochemistry; human; 1:10	Hawryluk E, Baran J, Gerami P, Sepehr A. 'Monster cell' melanoma with pulmonary metastasis and cyclin D1 amplification. J Cutan Pathol. 2013;40:61-5 pubmed publisher
immunohistochemistry; mouse; 1:100	Souza C, Gamba C, Campos C, Lopes M, Ferreira M, Andrade S, et al. Carboplatin delays mammary cancer 4T1 growth in mice. Pathol Res Pract. 2013;209:24-9 pubmed publisher
immunohistochemistry; human; 1:700	Naganuma S, Whelan K, Natsuizaka M, Kagawa S, Kinugasa H, Chang S, et al. Notch receptor inhibition reveals the importance of cyclin D1 and Wnt signaling in invasive esophageal squamous cell carcinoma. Am J Cancer Res. 2012;2:459-75 pubmed
western blot; human; 1:500	Wang J, Thiere M, Henne Bruns D, Knippschild U, Kornmann M. Inhibition of pancreatic cancer cell growth in vivo using a tetracycline-inducible cyclin D1 antisense expression system. Pancreas. 2013;42:141-8 pubmed publisher
western blot; human; 1:1000	Ramis G, Thomàs Moyà E, Fernandez De Mattos S, Rodriguez J, Villalonga P. EGFR inhibition in glioma cells modulates Rho signaling to inhibit cell motility and invasion and cooperates with temozolomide to reduce cell growth. PLoS ONE. 2012;7:e38770 pubmed publisher
western blot; mouse	Casimiro M, Crosariol M, Loro E, Ertel A, Yu Z, Dampier W, et al. ChIP sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice. J Clin Invest. 2012;122:833-43 pubmed publisher
western blot; mouse	Wang X, Sistrunk C, Miliani de Marval P, Kim Y, Rodriguez Puebla M. Combined effect of cyclin D3 expression and abrogation of cyclin D1 prevent mouse skin tumor development. Cell Cycle. 2012;11:335-42 pubmed publisher
immunohistochemistry; human; 1:50	Zhang L, Jiang F, Xu L, Wang J, Bai J, Yin R, et al. The role of cyclin D1 expression and patient's survival in non-small-cell lung cancer: a systematic review with meta-analysis. Clin Lung Cancer. 2012;13:188-95 pubmed publisher
western blot; human	Obrador Hevia A, Serra Sitjar M, Rodriguez J, Villalonga P, Fernandez De Mattos S. The tumour suppressor FOXO3 is a key regulator of mantle cell lymphoma proliferation and survival. Br J Haematol. 2012;156:334-45 pubmed publisher
western blot; human	Berdiel Acer M, Bohem M, Lopez Doriga A, Vidal A, Salazar R, Martínez Iniesta M, et al. Hepatic carcinoma-associated fibroblasts promote an adaptative response in colorectal cancer cells that inhibit proliferation and apoptosis: nonresistant cells die by nonapoptotic cell death. Neoplasia. 2011;13:931-46 pubmed
western blot; human	Gierut J, Zheng Y, Bie W, Carroll R, Ball Kell S, Haegebarth A, et al. Disruption of the mouse protein tyrosine kinase 6 gene prevents STAT3 activation and confers resistance to azoxymethane. Gastroenterology. 2011;141:1371-80, 1380.e1-2 pubmed publisher
immunohistochemistry; human; 1:200	Mellai M, Piazzi A, Caldera V, Monzeglio O, Cassoni P, Valente G, et al. IDH1 and IDH2 mutations, immunohistochemistry and associations in a series of brain tumors. J Neurooncol. 2011;105:345-57 pubmed publisher
immunoprecipitation; human western blot; human	Cerliani J, Guillardoy T, Giulianelli S, Vaque J, Gutkind J, Vanzulli S, et al. Interaction between FGFR-2, STAT5, and progesterone receptors in breast cancer. Cancer Res. 2011;71:3720-31 pubmed publisher
western blot; mouse	de Carcer G, Escobar B, Higuero A, Garcia L, Ansón A, Pérez G, et al. Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression. Mol Cell Biol. 2011;31:1225-39 pubmed publisher
western blot; human; 1:1000	Yap T, Walton M, Hunter L, Valenti M, de Haven Brandon A, Eve P, et al. Preclinical pharmacology, antitumor activity, and development of pharmacodynamic markers for the novel, potent AKT inhibitor CCT128930. Mol Cancer Ther. 2011;10:360-71 pubmed publisher
western blot; mouse; 1:500	Bottino M, Cerliani J, Rojas P, Giulianelli S, Soldati R, Mondillo C, et al. Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects. Breast Cancer Res Treat. 2011;126:621-36 pubmed publisher
western blot; rat; 1:1000	Tian Y, Liu Y, Chen X, Kang Q, Zhang J, Shi Q, et al. AMN082 promotes the proliferation and differentiation of neural progenitor cells with influence on phosphorylation of MAPK signaling pathways. Neurochem Int. 2010;57:8-15 pubmed publisher
western blot; human	Heiferman M, Salabat M, Ujiki M, Strouch M, Cheon E, Silverman R, et al. Sansalvamide induces pancreatic cancer growth arrest through changes in the cell cycle. Anticancer Res. 2010;30:73-8 pubmed
western blot; mouse; 1:500	Gonzalez Pardo V, Martin D, Gutkind J, Verstuyf A, Bouillon R, de Boland A, et al. 1 Alpha,25-dihydroxyvitamin D3 and its TX527 analog inhibit the growth of endothelial cells transformed by Kaposi sarcoma-associated herpes virus G protein-coupled receptor in vitro and in vivo. Endocrinology. 2010;151:23-31 pubmed publisher
western blot; rat; 1:1000	Chen X, Tian Y, Yao L, Zhang J, Liu Y. Hypoxia stimulates proliferation of rat neural stem cells with influence on the expression of cyclin D1 and c-Jun N-terminal protein kinase signaling pathway in vitro. Neuroscience. 2010;165:705-14 pubmed publisher
western blot; human	Lau C, Koty Z, Nalbantoglu J. Differential response of glioma cells to FOXO1-directed therapy. Cancer Res. 2009;69:5433-40 pubmed publisher
western blot; human	Li Y, Tan X, Dai C, Stolz D, Wang D, Liu Y. Inhibition of integrin-linked kinase attenuates renal interstitial fibrosis. J Am Soc Nephrol. 2009;20:1907-18 pubmed publisher
immunohistochemistry; human; 1:50	Sarkar R, Hunter I, Rajaganeshan R, Perry S, Guillou P, Jayne D. Expression of cyclin D2 is an independent predictor of the development of hepatic metastasis in colorectal cancer. Colorectal Dis. 2010;12:316-23 pubmed publisher
western blot; human	Ose R, Yanagawa T, Ikeda S, Ohara O, Koga H. PCDH24-induced contact inhibition involves downregulation of beta-catenin signaling. Mol Oncol. 2009;3:54-66 pubmed publisher
immunohistochemistry; human	Dong H, Weisberger J, Liu Z, Tugulea S. Immunophenotypic analysis of CD103+ B-lymphoproliferative disorders: hairy cell leukemia and its mimics. Am J Clin Pathol. 2009;131:586-95 pubmed publisher
immunohistochemistry; human; 1:50	Khoury T, Alrawi S, Ramnath N, Li Q, Grimm M, Black J, et al. Eukaryotic initiation factor-4E and cyclin D1 expression associated with patient survival in lung cancer. Clin Lung Cancer. 2009;10:58-66 pubmed publisher
western blot; mouse	Frémin C, Bessard A, Ezan F, Gailhouste L, Régeard M, Le Seyec J, et al. Multiple division cycles and long-term survival of hepatocytes are distinctly regulated by extracellular signal-regulated kinases ERK1 and ERK2. Hepatology. 2009;49:930-9 pubmed publisher
immunohistochemistry; human; 1:400	Vékony H, Roser K, Löning T, Raaphorst F, Leemans C, Van der Waal I, et al. Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands. Histopathology. 2008;53:658-66 pubmed publisher
western blot; human	Wang Z, Qi Y, Chen Q, Yang D, Tang S, Jin X, et al. Cyclin A is essential for the p53-modulated inhibition from benzo(a)pyrene toxicity in A549 cells. Toxicology. 2009;256:1-6 pubmed publisher
western blot; mouse; 1:50	Giulianelli S, Cerliani J, Lamb C, Fabris V, Bottino M, Gorostiaga M, et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int J Cancer. 2008;123:2518-31 pubmed publisher
immunoprecipitation; human western blot; human	LARREA M, Liang J, Da Silva T, Hong F, Shao S, Han K, et al. Phosphorylation of p27Kip1 regulates assembly and activation of cyclin D1-Cdk4. Mol Cell Biol. 2008;28:6462-72 pubmed publisher
western blot; human	Schnier J, Nishi K, Harley W, Gorin F. An acidic environment changes cyclin D1 localization and alters colony forming ability in gliomas. J Neurooncol. 2008;89:19-26 pubmed publisher
western blot; mouse	Zheng Y, Bie W, Yang R, Perekatt A, Poole A, Tyner A. Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine. Cancer Biol Ther. 2008;7:873-9 pubmed
immunohistochemistry; human	Sathyan K, Nalinakumari K, Abraham T, Kannan S. CCND1 polymorphisms (A870G and C1722G) modulate its protein expression and survival in oral carcinoma. Oral Oncol. 2008;44:689-97 pubmed
western blot; human; 1:1000	Qin X, Zhang H, Zhou X, Wang C, Zhang H, Zhang X, et al. Proliferation and migration mediated by Dkk-1/Wnt/beta-catenin cascade in a model of hepatocellular carcinoma cells. Transl Res. 2007;150:281-94 pubmed
immunoprecipitation; mouse western blot; mouse	Santamaria D, Barrière C, Cerqueira A, Hunt S, Tardy C, Newton K, et al. Cdk1 is sufficient to drive the mammalian cell cycle. Nature. 2007;448:811-5 pubmed
western blot; human	Yang D, Qi Y, Chen Q, Wang Z, Jin X, Gao J, et al. The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells. Mol Cell Biochem. 2007;304:219-26 pubmed
immunohistochemistry; human; 1:50	Lebe B, Pabuccuoglu U, Ozer E. The significance of Ki-67 proliferative index and cyclin D1 expression of dysplastic nevi in the biologic spectrum of melanocytic lesions. Appl Immunohistochem Mol Morphol. 2007;15:160-4 pubmed
western blot; mouse	Yang Z, Mott S, Rosmarin A. The Ets transcription factor GABP is required for cell-cycle progression. Nat Cell Biol. 2007;9:339-46 pubmed
western blot; human; 1:500	Wiseman D, Werner S, Crowell P. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. J Pharmacol Exp Ther. 2007;320:1163-70 pubmed
immunoprecipitation; human western blot; human	Qi Y, Tu Y, Yang D, Chen Q, Xiao J, Chen Y, et al. Cyclin A but not cyclin D1 is essential for c-myc-modulated cell-cycle progression. J Cell Physiol. 2007;210:63-71 pubmed
western blot; human	Paternot S, Dumont J, Roger P. Differential utilization of cyclin D1 and cyclin D3 in the distinct mitogenic stimulations by growth factors and TSH of human thyrocytes in primary culture. Mol Endocrinol. 2006;20:3279-92 pubmed
western blot; rat	Shan Y, Zhou W, Fu X, Yan H, Yang W, Liu S, et al. The role of p28GANK in rat oval cells activation and proliferation. Liver Int. 2006;26:240-7 pubmed
western blot; human	Toschi E, Bacigalupo I, Strippoli R, Chiozzini C, Cereseto A, Falchi M, et al. HIV-1 Tat regulates endothelial cell cycle progression via activation of the Ras/ERK MAPK signaling pathway. Mol Biol Cell. 2006;17:1985-94 pubmed
immunohistochemistry; human	Pawarode A, Baer M, Padmanabhan S, Wallace P, Barcos M, Sait S, et al. Simultaneous presentation of acute monoblastic leukemia and mantle cell lymphoma: case report and review of the literature. Leuk Lymphoma. 2005;46:1813-8 pubmed
immunohistochemistry; human western blot; human	Hui A, Or Y, Takano H, Tsang R, To K, Guan X, et al. Array-based comparative genomic hybridization analysis identified cyclin D1 as a target oncogene at 11q13.3 in nasopharyngeal carcinoma. Cancer Res. 2005;65:8125-33 pubmed
western blot; human	Mermelshtein A, Gerson A, Walfisch S, Delgado B, Shechter Maor G, Delgado J, et al. Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas. Br J Cancer. 2005;93:338-45 pubmed
western blot; human; 1:200	Raynaud F, Whittaker S, Fischer P, McClue S, Walton M, Barrie S, et al. In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202. Clin Cancer Res. 2005;11:4875-87 pubmed
immunohistochemistry; human; 1:20	Schmitt Graeff A, Ertelt Heitzmann V, Allgaier H, Olschewski M, Nitschke R, Haxelmans S, et al. Coordinated expression of cyclin D1 and LEF-1/TCF transcription factor is restricted to a subset of hepatocellular carcinoma. Liver Int. 2005;25:839-47 pubmed
	Kossatz U, Dietrich N, Zender L, Buer J, Manns M, Malek N. Skp2-dependent degradation of p27kip1 is essential for cell cycle progression. Genes Dev. 2004;18:2602-7 pubmed
	Jirawatnotai S, Aziyu A, Osmundson E, Moons D, Zou X, Kineman R, et al. Cdk4 is indispensable for postnatal proliferation of the anterior pituitary. J Biol Chem. 2004;279:51100-6 pubmed
	Vouret Craviari V, Boulter E, Grall D, Matthews C, Van Obberghen Schilling E. ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells. J Cell Sci. 2004;117:4559-69 pubmed
	Kirma N, Luthra R, Jones J, Liu Y, Nair H, Mandava U, et al. Overexpression of the colony-stimulating factor (CSF-1) and/or its receptor c-fms in mammary glands of transgenic mice results in hyperplasia and tumor formation. Cancer Res. 2004;64:4162-70 pubmed
	Takeda A, Osaki M, Adachi K, Honjo S, Ito H. Role of the phosphatidylinositol 3'-kinase-Akt signal pathway in the proliferation of human pancreatic ductal carcinoma cell lines. Pancreas. 2004;28:353-8 pubmed
	Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari K, Abraham T, et al. p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma. Int J Cancer. 2004;109:710-6 pubmed
	van den Brink G, Bleuming S, Hardwick J, Schepman B, Offerhaus G, Keller J, et al. Indian Hedgehog is an antagonist of Wnt signaling in colonic epithelial cell differentiation. Nat Genet. 2004;36:277-82 pubmed
	Whittaker S, Walton M, Garrett M, Workman P. The Cyclin-dependent kinase inhibitor CYC202 (R-roscovitine) inhibits retinoblastoma protein phosphorylation, causes loss of Cyclin D1, and activates the mitogen-activated protein kinase pathway. Cancer Res. 2004;64:262-72 pubmed
	Faast R, White J, Cartwright P, Crocker L, Sarcevic B, Dalton S. Cdk6-cyclin D3 activity in murine ES cells is resistant to inhibition by p16(INK4a). Oncogene. 2004;23:491-502 pubmed
	Cui X, Li S, Shraim A, Kobayashi Y, Hayakawa T, Kanno S, et al. Subchronic exposure to arsenic through drinking water alters expression of cancer-related genes in rat liver. Toxicol Pathol. 2004;32:64-72 pubmed
	Haven C, van Puijenbroek M, Karperien M, Fleuren G, Morreau H. Differential expression of the calcium sensing receptor and combined loss of chromosomes 1q and 11q in parathyroid carcinoma. J Pathol. 2004;202:86-94 pubmed
	Doglioni C, Piccinin S, Demontis S, Cangi M, Pecciarini L, Chiarelli C, et al. Alterations of beta-catenin pathway in non-melanoma skin tumors: loss of alpha-ABC nuclear reactivity correlates with the presence of beta-catenin gene mutation. Am J Pathol. 2003;163:2277-87 pubmed
	Chen B, van den Brekel M, Buschers W, Balm A, van Velthuysen M. Validation of tissue array technology in head and neck squamous cell carcinoma. Head Neck. 2003;25:922-30 pubmed
	Papeleu P, Loyer P, Vanhaecke T, Elaut G, Geerts A, Guguen Guillouzo C, et al. Trichostatin A induces differential cell cycle arrests but does not induce apoptosis in primary cultures of mitogen-stimulated rat hepatocytes. J Hepatol. 2003;39:374-82 pubmed
	de Leng W, Westerman A, Weterman M, De Rooij F, Dekken Hv H, de Goeij A, et al. Cyclooxygenase 2 expression and molecular alterations in Peutz-Jeghers hamartomas and carcinomas. Clin Cancer Res. 2003;9:3065-72 pubmed
	Gingras B, Rodier G, Giasson E, Coulombe P, Chassagne C, Meloche S. Expression of angiotensin type II receptor downregulates Cdk4 synthesis and inhibits cell-cycle progression. Oncogene. 2003;22:2633-42 pubmed
	Vijapurkar U, Kim M, Koland J. Roles of mitogen-activated protein kinase and phosphoinositide 3'-kinase in ErbB2/ErbB3 coreceptor-mediated heregulin signaling. Exp Cell Res. 2003;284:291-302 pubmed
	Chen B, Pollard J. Cyclin D2 compensates for the loss of cyclin D1 in estrogen-induced mouse uterine epithelial cell proliferation. Mol Endocrinol. 2003;17:1368-81 pubmed
	Rodrigues N, Dillon D, Carter D, Parisot N, Haffty B. Differences in the pathologic and molecular features of intraductal breast carcinoma between younger and older women. Cancer. 2003;97:1393-403 pubmed
	Fadare O, Shukla P. Another case of mantle cell lymphoma presenting as breast masses. J Clin Pathol. 2002;55:640 pubmed
	Ecarnot Laubriet A, Assem M, Poirson Bichat F, Moisant M, Bernard C, Lecour S, et al. Stage-dependent activation of cell cycle and apoptosis mechanisms in the right ventricle by pressure overload. Biochim Biophys Acta. 2002;1586:233-42 pubmed
	Bearss D, Lee R, Troyer D, Pestell R, Windle J. Differential effects of p21(WAF1/CIP1) deficiency on MMTV-ras and MMTV-myc mammary tumor properties. Cancer Res. 2002;62:2077-84 pubmed
	Beier F, Ali Z, Mok D, Taylor A, Leask T, Albanese C, et al. TGFbeta and PTHrP control chondrocyte proliferation by activating cyclin D1 expression. Mol Biol Cell. 2001;12:3852-63 pubmed
	Wagner M, Hampel B, Hutter E, Pfister G, Krek W, Zwerschke W, et al. Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2. Exp Gerontol. 2001;37:41-55 pubmed
	Lewis J, Thomas T, Klinge C, Gallo M, Thomas T. Regulation of cell cycle and cyclins by 16alpha-hydroxyestrone in MCF-7 breast cancer cells. J Mol Endocrinol. 2001;27:293-307 pubmed
	Vogt B, Rossman T. Effects of arsenite on p53, p21 and cyclin D expression in normal human fibroblasts -- a possible mechanism for arsenite's comutagenicity. Mutat Res. 2001;478:159-68 pubmed
	Reutens A, Fu M, Wang C, Albanese C, McPhaul M, Sun Z, et al. Cyclin D1 binds the androgen receptor and regulates hormone-dependent signaling in a p300/CBP-associated factor (P/CAF)-dependent manner. Mol Endocrinol. 2001;15:797-811 pubmed
	Schulze A, Lehmann K, Jefferies H, McMahon M, Downward J. Analysis of the transcriptional program induced by Raf in epithelial cells. Genes Dev. 2001;15:981-94 pubmed
	Kirma N, Gill K, Mandava U, Tekmal R. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice. Cancer Res. 2001;61:1910-8 pubmed
	Dunphy C, Perkins S. Large cell variants of CD5+, CD23- B-cell lymphoma/leukemia. Arch Pathol Lab Med. 2001;125:513-8 pubmed
	Soria J, Morat L, Commo F, Dabit D, Perie S, Sabatier L, et al. Telomerase activation cooperates with inactivation of p16 in early head and neck tumorigenesis. Br J Cancer. 2001;84:504-11 pubmed
	de Alboran I, O Hagan R, Gartner F, Malynn B, Davidson L, Rickert R, et al. Analysis of C-MYC function in normal cells via conditional gene-targeted mutation. Immunity. 2001;14:45-55 pubmed
	Tong W, Pollard J. Genetic evidence for the interactions of cyclin D1 and p27(Kip1) in mice. Mol Cell Biol. 2001;21:1319-28 pubmed
	Hui A, Li X, Shi Y, Takayama T, Torzilli G, Makuuchi M. Cyclin D1 overexpression is a critical event in gallbladder carcinogenesis and independently predicts decreased survival for patients with gallbladder carcinoma. Clin Cancer Res. 2000;6:4272-7 pubmed
	Geradts J, Ingram C. Abnormal expression of cell cycle regulatory proteins in ductal and lobular carcinomas of the breast. Mod Pathol. 2000;13:945-53 pubmed
	Clark W, Black E, MacLaren A, Kruse U, LaThangue N, Vogt P, et al. v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity. Mol Cell Biol. 2000;20:2529-42 pubmed
	Tsutsui T, Hesabi B, Moons D, Pandolfi P, Hansel K, Koff A, et al. Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity. Mol Cell Biol. 1999;19:7011-9 pubmed
	Collecchi P, Passoni A, Rocchetta M, Gnesi E, Baldini E, Bevilacqua G. Cyclin-D1 expression in node-positive (N+) and node-negative (N-) infiltrating human mammary carcinomas. Int J Cancer. 1999;84:139-44 pubmed
	Tong W, Pollard J. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biol. 1999;19:2251-64 pubmed
	Zwijsen R, Buckle R, Hijmans E, Loomans C, Bernards R. Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998;12:3488-98 pubmed
	Schulze A, Mannhardt B, Zerfass Thome K, Zwerschke W, Jansen Durr P. Anchorage-independent transcription of the cyclin A gene induced by the E7 oncoprotein of human papillomavirus type 16. J Virol. 1998;72:2323-34 pubmed
	Kornmann M, Arber N, Korc M. Inhibition of basal and mitogen-stimulated pancreatic cancer cell growth by cyclin D1 antisense is associated with loss of tumorigenicity and potentiation of cytotoxicity to cisplatinum. J Clin Invest. 1998;101:344-52 pubmed
	Xiong W, Pestell R, Rosner M. Role of cyclins in neuronal differentiation of immortalized hippocampal cells. Mol Cell Biol. 1997;17:6585-97 pubmed
	Neuman E, Ladha M, Lin N, Upton T, Miller S, DiRenzo J, et al. Cyclin D1 stimulation of estrogen receptor transcriptional activity independent of cdk4. Mol Cell Biol. 1997;17:5338-47 pubmed
	Reynisdottir I, Massague J. The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2. Genes Dev. 1997;11:492-503 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin D1 Monoclonal Antibody (DCS-6), Biotin

Catalog # :

MA5-12699

Quantity :

500µL

Price :

US 375.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Non-human primate, Rat

Applications :

Flow Cytometry: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Canine, Human, Mouse, Non-human primate, Rat

Clone :

DCS-6

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

Immunogen :

Human recombinant full length cyclin D1 protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

B-cell CLL/lymphoma 1, B-cell lymphoma 1 protein, BCL-1 oncogene, G1/S-specific cyclin D1, G1/S-specific cyclin-D1, PRAD1 oncogene