Progesterone Receptor Monoclonal Antibody (hPRa 7) | Invitrogen MA5-12658 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Progesterone Receptor Monoclonal Antibody (hPRa 7)

catalog :

MA5-12658

quantity :

500 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

hPRa 7

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; fig 4	Joshi P, Waterhouse P, Kannan N, Narala S, Fang H, Di Grappa M, et al. RANK Signaling Amplifies WNT-Responsive Mammary Progenitors through R-SPONDIN1. Stem Cell Reports. 2015;5:31-44 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50	Chen F, Li A, Gao S, Hollern D, Williams M, Liu F, et al. Tip30 controls differentiation of murine mammary luminal progenitor to estrogen receptor-positive luminal cell through regulating FoxA1 expression. Cell Death Dis. 2014;5:e1242 pubmed publisher
immunocytochemistry; mouse	Melchor L, Molyneux G, Mackay A, Magnay F, Atienza M, Kendrick H, et al. Identification of cellular and genetic drivers of breast cancer heterogeneity in genetically engineered mouse tumour models. J Pathol. 2014;233:124-37 pubmed publisher
western blot; mouse	Spallanzani R, Dalotto Moreno T, Raffo Iraolagoitia X, Ziblat A, Domaica C, Avila D, et al. Expansion of CD11b(+)Ly6G (+)Ly6C (int) cells driven by medroxyprogesterone acetate in mice bearing breast tumors restrains NK cell effector functions. Cancer Immunol Immunother. 2013;62:1781-95 pubmed publisher
immunohistochemistry; mouse; 1:50	Aupperlee M, Leipprandt J, Bennett J, Schwartz R, Haslam S. Amphiregulin mediates progesterone-induced mammary ductal development during puberty. Breast Cancer Res. 2013;15:R44 pubmed publisher
western blot; human	Rivas M, Venturutti L, Huang Y, Schillaci R, Huang T, Elizalde P. Downregulation of the tumor-suppressor miR-16 via progestin-mediated oncogenic signaling contributes to breast cancer development. Breast Cancer Res. 2012;14:R77 pubmed
western blot; human; 4 ug/ml; fig 5	Tieszen C, Goyeneche A, Brandhagen B, Ortbahn C, Telleria C. Antiprogestin mifepristone inhibits the growth of cancer cells of reproductive and non-reproductive origin regardless of progesterone receptor expression. BMC Cancer. 2011;11:207 pubmed publisher
immunohistochemistry; mouse	Welsh J, Zinser L, Mianecki Morton L, Martin J, Waltz S, James H, et al. Age-related changes in the epithelial and stromal compartments of the mammary gland in normocalcemic mice lacking the vitamin D3 receptor. PLoS ONE. 2011;6:e16479 pubmed publisher
immunoprecipitation; human immunohistochemistry; human; 1:100	Kariagina A, Xie J, Leipprandt J, Haslam S. Amphiregulin mediates estrogen, progesterone, and EGFR signaling in the normal rat mammary gland and in hormone-dependent rat mammary cancers. Horm Cancer. 2010;1:229-44 pubmed publisher
western blot; human	Proietti C, Beguelin W, Flaqué M, Cayrol F, Rivas M, Tkach M, et al. Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. Steroids. 2011;76:381-92 pubmed publisher
immunohistochemistry; mouse; 1:50	Zhang C, Mori M, Gao S, Li A, Hoshino I, Aupperlee M, et al. Tip30 deletion in MMTV-Neu mice leads to enhanced EGFR signaling and development of estrogen receptor-positive and progesterone receptor-negative mammary tumors. Cancer Res. 2010;70:10224-33 pubmed publisher
chromatin immunoprecipitation; mouse	Beguelin W, Díaz Flaqué M, Proietti C, Cayrol F, Rivas M, Tkach M, et al. Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. Mol Cell Biol. 2010;30:5456-72 pubmed publisher
immunocytochemistry; mouse; 1:100 western blot; mouse; 1:100	Bottino M, Cerliani J, Rojas P, Giulianelli S, Soldati R, Mondillo C, et al. Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects. Breast Cancer Res Treat. 2011;126:621-36 pubmed publisher
immunohistochemistry; mouse; 1:200 western blot; mouse; 2 ug/ml	Wargon V, Fernandez S, Goin M, Giulianelli S, Russo J, Lanari C. Hypermethylation of the progesterone receptor A in constitutive antiprogestin-resistant mouse mammary carcinomas. Breast Cancer Res Treat. 2011;126:319-32 pubmed publisher
immunohistochemistry; mouse	Soldati R, Wargon V, Cerliani J, Giulianelli S, Vanzulli S, Gorostiaga M, et al. Inhibition of mammary tumor growth by estrogens: is there a specific role for estrogen receptors alpha and beta?. Breast Cancer Res Treat. 2010;123:709-24 pubmed publisher
immunocytochemistry; human; 1:100	Salama S, Kamel M, Botting S, Salih S, Borahay M, Hamed A, et al. Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells. PLoS ONE. 2009;4:e7356 pubmed publisher
western blot; human	Proietti C, Rosemblit C, Beguelin W, Rivas M, Díaz Flaqué M, Charreau E, et al. Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth. Mol Cell Biol. 2009;29:1249-65 pubmed publisher
immunohistochemistry; mouse; 1:50	Giulianelli S, Cerliani J, Lamb C, Fabris V, Bottino M, Gorostiaga M, et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int J Cancer. 2008;123:2518-31 pubmed publisher
flow cytometry; human immunocytochemistry; human; 1:50 western blot; human; 2 ug/ml	Arruvito L, Giulianelli S, Flores A, Paladino N, Barboza M, Lanari C, et al. NK cells expressing a progesterone receptor are susceptible to progesterone-induced apoptosis. J Immunol. 2008;180:5746-53 pubmed
immunohistochemistry; human; 1:50	Hoogendoorn W, Hollema H, van Boven H, Bergman E, de Leeuw Mantel G, Platteel I, et al. Prognosis of uterine corpus cancer after tamoxifen treatment for breast cancer. Breast Cancer Res Treat. 2008;112:99-108 pubmed
immunocytochemistry; human	Chi J, Rodriguez E, Wang Z, Nuyten D, Mukherjee S, van de Rijn M, et al. Gene expression programs of human smooth muscle cells: tissue-specific differentiation and prognostic significance in breast cancers. PLoS Genet. 2007;3:1770-84 pubmed
immunohistochemistry; rat; 1:50, 1:100	Kariagina A, Aupperlee M, Haslam S. Progesterone receptor isoforms and proliferation in the rat mammary gland during development. Endocrinology. 2007;148:2723-36 pubmed
immunohistochemistry; human; 1:200	Ishizuka M, Hatori M, Dohi O, Suzuki T, Miki Y, Tazawa C, et al. Expression profiles of sex steroid receptors in desmoid tumors. Tohoku J Exp Med. 2006;210:189-98 pubmed
immunoprecipitation; mouse immunocytochemistry; mouse; 1:100 western blot; mouse	Salatino M, Beguelin W, Peters M, Carnevale R, Proietti C, Galigniana M, et al. Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation. Oncogene. 2006;25:7723-39 pubmed
immunohistochemistry; human; 1:100	Akahane T, Sekizawa A, Okuda T, Kushima M, Saito H, Okai T. Disappearance of steroid hormone dependency during malignant transformation of ovarian clear cell cancer. Int J Gynecol Pathol. 2005;24:369-76 pubmed
immunohistochemistry; human; 1:100	Yamashita H, Nishio M, Kobayashi S, Ando Y, Sugiura H, Zhang Z, et al. Phosphorylation of estrogen receptor alpha serine 167 is predictive of response to endocrine therapy and increases postrelapse survival in metastatic breast cancer. Breast Cancer Res. 2005;7:R753-64 pubmed
immunohistochemistry; human; 1:100	Oh S, Kim C, Park I, Romero R, Sohn Y, Moon K, et al. Progesterone receptor isoform (A/B) ratio of human fetal membranes increases during term parturition. Am J Obstet Gynecol. 2005;193:1156-60 pubmed
immunohistochemistry; mouse western blot; mouse	Proietti C, Salatino M, Rosemblit C, Carnevale R, Pecci A, Kornblihtt A, et al. Progestins induce transcriptional activation of signal transducer and activator of transcription 3 (Stat3) via a Jak- and Src-dependent mechanism in breast cancer cells. Mol Cell Biol. 2005;25:4826-40 pubmed
immunohistochemistry; human	Hasegawa J, Yanaihara A, Iwasaki S, Otsuka Y, Negishi M, Akahane T, et al. Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality. Hum Reprod. 2005;20:2194-200 pubmed
immunohistochemistry; human; 1:100	Nishimura Y, Watanabe J, Jobo T, Hattori M, Arai T, Kuramoto H. Cytologic scoring of endometrioid adenocarcinoma of the endometrium. Cancer. 2005;105:8-12 pubmed
	Ponikwicka Tyszko D, Chruściel M, Pulawska K, Bernaczyk P, Sztachelska M, Guo P, et al. Mifepristone Treatment Promotes Testicular Leydig Cell Tumor Progression in Transgenic Mice. Cancers (Basel). 2020;12: pubmed publisher
	Ponikwicka Tyszko D, Chruściel M, Stelmaszewska J, Bernaczyk P, Chrusciel P, Sztachelska M, et al. Molecular mechanisms underlying mifepristone's agonistic action on ovarian cancer progression. EBioMedicine. 2019;47:170-183 pubmed publisher
	Dinh D, Breen J, Akison L, DeMayo F, Brown H, Robker R, et al. Tissue-specific progesterone receptor-chromatin binding and the regulation of progesterone-dependent gene expression. Sci Rep. 2019;9:11966 pubmed publisher
	Grindstad T, Richardsen E, Andersen S, Skjefstad K, Rakaee Khanehkenari M, Donnem T, et al. Progesterone Receptors in Prostate Cancer: Progesterone receptor B is the isoform associated with disease progression. Sci Rep. 2018;8:11358 pubmed publisher
	Salatino M, Schillaci R, Proietti C, Carnevale R, Frahm I, Molinolo A, et al. Inhibition of in vivo breast cancer growth by antisense oligodeoxynucleotides to type I insulin-like growth factor receptor mRNA involves inactivation of ErbBs, PI-3K/Akt and p42/p44 MAPK signaling pathways but not modulation of progesterone receptor. Oncogene. 2004;23:5161-74 pubmed
	Abd Elaziz M, Akahira J, Moriya T, Suzuki T, Yaegashi N, Sasano H. Nuclear receptor DAX-1 in human common epithelial ovarian carcinoma: an independent prognostic factor of clinical outcome. Cancer Sci. 2003;94:980-5 pubmed
	Vermeulen M, Pazos P, Lanari C, Molinolo A, Gamberale R, Geffner J, et al. Medroxyprogesterone acetate enhances in vivo and in vitro antibody production. Immunology. 2001;104:80-6 pubmed
	Kirma N, Gill K, Mandava U, Tekmal R. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice. Cancer Res. 2001;61:1910-8 pubmed
	O Brien S, Welter B, Mantzke K, Price T. Identification of progesterone receptor in human subcutaneous adipose tissue. J Clin Endocrinol Metab. 1998;83:509-13 pubmed

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image 1 :

product information

Product Type :

Antibody

Product Name :

Progesterone Receptor Monoclonal Antibody (hPRa 7)

Catalog # :

MA5-12658

Quantity :

500 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1:50, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Mouse, Non-human primate, Rat

Clone :

hPRa 7

Isotype :

IgG1, kappa

Storage :

4° C

Description :

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

Immunogen :

PgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product

more info or order :

Invitrogen product webpage