NPM1 Monoclonal Antibody (NA24) | Invitrogen MA5-12508 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NPM1 Monoclonal Antibody (NA24)

catalog :

MA5-12508

quantity :

500 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NA24

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
ELISA; human; loading ...; fig s3	Kourentzi K, Crum M, Patil U, Prebisch A, Chavan D, Vu B, et al. Recombinant expression, characterization, and quantification in human cancer cell lines of the Anaplastic Large-Cell Lymphoma-characteristic NPM-ALK fusion protein. Sci Rep. 2020;10:5078 pubmed publisher
	Lee K, Zhang P, Kim H, Mitrea D, Sarkar M, Freibaum B, et al. C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. Cell. 2016;167:774-788.e17 pubmed publisher
	Leal M, Mazzotti T, Calcagno D, Cirilo P, Martinez M, Demachki S, et al. Deregulated expression of Nucleophosmin 1 in gastric cancer and its clinicopathological implications. BMC Gastroenterol. 2014;14:9 pubmed publisher
	Zimmerman D, Boddy C, Schoenherr C. Oct4/Sox2 binding sites contribute to maintaining hypomethylation of the maternal igf2/h19 imprinting control region. PLoS ONE. 2013;8:e81962 pubmed publisher
	Sokoloski K, Dickson A, Chaskey E, Garneau N, Wilusz C, Wilusz J. Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and mosquito cells. Cell Host Microbe. 2010;8:196-207 pubmed publisher
	Coutinho Camillo C, Lourenço S, Nishimoto I, Kowalski L, Soares F. Nucleophosmin, p53, and Ki-67 expression patterns on an oral squamous cell carcinoma tissue microarray. Hum Pathol. 2010;41:1079-86 pubmed publisher
	Wang P, Wu F, Ma Y, Li L, Lai R, Young L. Functional characterization of the kinase activation loop in nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) using tandem affinity purification and liquid chromatography-mass spectrometry. J Biol Chem. 2010;285:95-103 pubmed publisher

product information

Product Type :

Antibody

Product Name :

NPM1 Monoclonal Antibody (NA24)

Catalog # :

MA5-12508

Quantity :

500 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ChIP assay: 2.5 µg/10^6 cells, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1-2 µg/mL, Western Blot: 1:100-1:1,000

Species :

Human, Mouse

Clone :

NA24

Isotype :

IgG, kappa

Storage :

4° C

Description :

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

Immunogen :

GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 aa of ALK protein

Format :

Liquid

Applications w/Dilutions :

ChIP assay: 2.5 µg/10^6 cells, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1-2 µg/mL, Western Blot: 1:100-1:1,000

Aliases :

B23; B23 antibody; B23NP; MGC104254; mNPM antibody; mNPM1 antibody; NO38; NO38 antibody; Npm; npm1; npm1.L; Nucleolar phosphoprotein B23; nucleolar protein B23.1; nucleolar protein B23/No38; nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog; nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; nucleoplasmin; numatrin; numatrin antibody; testicular tissue protein Li 128; unnamed protein product; XELAEV_18020022mg

more info or order :

Invitrogen product webpage