Perforin Monoclonal Antibody (5B10) | Invitrogen MA5-12469 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Perforin Monoclonal Antibody (5B10)

catalog :

MA5-12469

quantity :

500 µL

price :

US 456.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B10

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; tbl 3	Torrelo A, Noguera Morel L, Hernandez Martin A, Clemente D, Barja J, Buzon L, et al. Recurrent lipoatrophic panniculitis of children. J Eur Acad Dermatol Venereol. 2017;31:536-543 pubmed publisher
immunohistochemistry; human	Fugier E, Marche H, Thelu M, Macek Jilkova Z, Van Campenhout N, Dufeu Duchesne T, et al. Functions of liver natural killer cells are dependent on the severity of liver inflammation and fibrosis in chronic hepatitis C. PLoS ONE. 2014;9:e95614 pubmed publisher
immunohistochemistry; human	Abdou A, Shoeib M, Bakry O, El Bality H. Immunohistochemical expression of granzyme B and perforin in discoid lupus erythematosus. Ultrastruct Pathol. 2013;37:408-16 pubmed publisher
immunohistochemistry; human	Pagni F, Isimbaldi G, Vergani F, Casiraghi P, Marzorati L, Migliorino G, et al. Primary angiitis of the central nervous system: 2 atypical cases. Folia Neuropathol. 2012;50:293-9 pubmed
immunohistochemistry; human; 1:10	Milani V, Stangl S, Issels R, Gehrmann M, Wagner B, Hube K, et al. Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report. J Transl Med. 2009;7:50 pubmed publisher
immunohistochemistry; human	Dunning K, Wudhikarn K, Safo A, Holman C, McKenna R, Pambuccian S. Adrenal extranodal NK/T-cell lymphoma diagnosed by fine-needle aspiration and cerebrospinal fluid cytology and immunophenotyping: a case report. Diagn Cytopathol. 2009;37:686-95 pubmed publisher
immunohistochemistry; human	van Es L, De Heer E, Vleming L, van der Wal A, Mallat M, Bajema I, et al. GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy. Kidney Int. 2008;73:1426-33 pubmed publisher
	Wang L, Wang G, Gao T. Acneiform primary cutaneous CD4-positive small/medium pleomorphic T-cell lymphoma with prominent necrosis. J Cutan Pathol. 2015;42:265-70 pubmed publisher

image

image 1 :

Formalin-fixed, paraffin-embedded human lymphoma stained with Perforin antibody using peroxidase-conjugate and AEC chromogen. Note granular cytoplasmic staining of lymphocytes.

product information

Product Type :

Antibody

Product Name :

Perforin Monoclonal Antibody (5B10)

Catalog # :

MA5-12469

Quantity :

500 µL

Price :

US 456.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 1:10, Western Blot: 1:500

Species :

Human

Clone :

5B10

Isotype :

IgG1

Storage :

4° C

Description :

Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

Immunogen :

Recombinant protein corresponding to C-terminal region of human perforin

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:10, Western Blot: 1:500

Aliases :

Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3

more info or order :

Invitrogen product webpage