p53 Monoclonal Antibody (PAb 122) | Invitrogen MA5-12453 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p53 Monoclonal Antibody (PAb 122)

catalog :

MA5-12453

quantity :

500 µL

price :

US 449.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAb 122

reactivity :

Chinese hamsters, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; 1:1000; fig 11 western blot; rat; fig s6	Lin S, Gou G, Hsia C, Ho C, Huang K, Wu Y, et al. Simulated Microgravity Disrupts Cytoskeleton Organization and Increases Apoptosis of Rat Neural Crest Stem Cells Via Upregulating CXCR4 Expression and RhoA-ROCK1-p38 MAPK-p53 Signaling. Stem Cells Dev. 2016;25:1172-93 pubmed publisher
western blot; mouse; 1:500	Tanaka T, Okada M, Hozumi Y, Tachibana K, Kitanaka C, Hamamoto Y, et al. Cytoplasmic localization of DGK? exerts a protective effect against p53-mediated cytotoxicity. J Cell Sci. 2013;126:2785-97 pubmed publisher
western blot; human	Cai B, Hsu P, Hsin I, Chao C, Lu M, Lin H, et al. p53 acts as a co-repressor to regulate keratin 14 expression during epidermal cell differentiation. PLoS ONE. 2012;7:e41742 pubmed publisher
western blot; mouse	Kasper J, Arai T, DeCaprio J. A novel p53-binding domain in CUL7. Biochem Biophys Res Commun. 2006;348:132-8 pubmed
immunoprecipitation; human	Borger D, DeCaprio J. Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. J Virol. 2006;80:4292-303 pubmed
immunoprecipitation; mouse	di Masi A, Antoccia A, Dimauro I, Argentino Storino A, Mosiello A, Mango R, et al. Gene expression and apoptosis induction in p53-heterozygous irradiated mice. Mutat Res. 2006;594:49-62 pubmed
	Soto Mercado V, Mendivil Perez M, Velez Pardo C, Lopera F, Jimenez Del Rio M. Cholinergic-like neurons carrying PSEN1 E280A mutation from familial Alzheimer's disease reveal intraneuronal sAPPβ fragments accumulation, hyperphosphorylation of TAU, oxidative stress, apoptosis and Ca2+ dysregulation: Therapeutic implications. PLoS ONE. 2020;15:e0221669 pubmed publisher
	Paredes J, Ji P, Lacomb J, Shroyer K, Martello L, Williams J. Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity. Int J Oncol. 2018;53:1516-1528 pubmed publisher
	Liu F, Ma F, Wang Y, Hao L, Zeng H, Jia C, et al. PKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis. Nat Cell Biol. 2017;19:1358-1370 pubmed publisher
	Truksa J, Kovar J, Valenta T, Ehrlichova M, Polak J, Naumann P. Iron deprivation induces apoptosis independently of p53 in human and murine tumour cells. Cell Prolif. 2003;36:199-213 pubmed
	Ratovitski E, Patturajan M, Hibi K, Trink B, Yamaguchi K, Sidransky D. p53 associates with and targets Delta Np63 into a protein degradation pathway. Proc Natl Acad Sci U S A. 2001;98:1817-22 pubmed
	Venkatachalam S, Shi Y, Jones S, Vogel H, Bradley A, Pinkel D, et al. Retention of wild-type p53 in tumors from p53 heterozygous mice: reduction of p53 dosage can promote cancer formation. EMBO J. 1998;17:4657-67 pubmed

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image 1 :

product information

Product Type :

Antibody

Product Name :

p53 Monoclonal Antibody (PAb 122)

Catalog # :

MA5-12453

Quantity :

500 µL

Price :

US 449.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Hamster, Human, Mouse, Non-human primate, Rat

Applications :

Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL

Species :

Hamster, Human, Mouse, Non-human primate, Rat

Clone :

PAb 122

Isotype :

IgG2b, kappa

Storage :

4° C

Description :

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

Immunogen :

SV40-transformed mouse B4 cells

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL

Aliases :

Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3

more info or order :

Invitrogen product webpage