EpCAM Monoclonal Antibody (323/A3) | Invitrogen MA5-12436 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EpCAM Monoclonal Antibody (323/A3)

catalog :

MA5-12436

quantity :

500 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

323/A3

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, other

more info or order :

Invitrogen product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
other; human; tbl 1	Schasfoort R, Andree K, van der Velde N, van der Kooi A, StojanoviÄ‡ I, Terstappen L. Interpolation method for accurate affinity ranking of arrayed ligand-analyte interactions. Anal Biochem. 2016;500:21-3 pubmed publisher
flow cytometry; human; 5 ug/ml	Hsieh C, Chen H, Chang Y, Pang S, Kuo M, Chuang C, et al. Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies. Anticancer Res. 2013;33:4875-89 pubmed
immunohistochemistry; human; 1:500	Turner R, Mendel G, Wauthier E, Barbier C, Reid L. Hyaluronan-supplemented buffers preserve adhesion mechanisms facilitating cryopreservation of human hepatic stem/progenitor cells. Cell Transplant. 2012;21:2257-66 pubmed publisher
immunocytochemistry; human; 2.5 ug/ml	Eifler R, Lind J, Falkenhagen D, Weber V, Fischer M, Zeillinger R. Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept. Cytometry B Clin Cytom. 2011;80:100-11 pubmed publisher
flow cytometry; human immunocytochemistry; human	Lin J, Liao S, Lee E, Hung M, Sayion Y, Chen H, et al. Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome. J Biomed Sci. 2009;16:105 pubmed publisher
immunohistochemistry; human	Bellone S, Siegel E, Cocco E, Cargnelutti M, Silasi D, Azodi M, et al. Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy. Int J Gynecol Cancer. 2009;19:860-6 pubmed publisher
western blot; human	Neighbors M, Apt D, Chang J, Brinkman A, Sipos Solman I, Ong R, et al. EpCAM-specific vaccine response by modified antigen and chimeric costimulatory molecule in cynomolgus monkeys. J Immunother. 2008;31:644-55 pubmed publisher
immunocytochemistry; human	Sequeira S, Ranganathan A, Adam A, Iglesias B, Farias E, Aguirre Ghiso J. Inhibition of proliferation by PERK regulates mammary acinar morphogenesis and tumor formation. PLoS ONE. 2007;2:e615 pubmed
flow cytometry; mouse	Elia L, Mennuni C, Storto M, Podda S, Calvaruso F, Salucci V, et al. Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers. Eur J Immunol. 2006;36:1337-49 pubmed publisher
	Motomura K, Romero R, Xu Y, Theis K, Galaz J, Winters A, et al. Intra-Amniotic Infection with Ureaplasma parvum Causes Preterm Birth and Neonatal Mortality That Are Prevented by Treatment with Clarithromycin. MBio. 2020;11: pubmed publisher
	Libanje F, Raingeaud J, Luan R, Thomas Z, Zajac O, Veiga J, et al. ROCK2 inhibition triggers the collective invasion of colorectal adenocarcinomas. EMBO J. 2019;38:e99299 pubmed publisher
	Senger S, Ingano L, Freire R, Anselmo A, Zhu W, Sadreyev R, et al. Human Fetal-Derived Enterospheres Provide Insights on Intestinal Development and a Novel Model to Study Necrotizing Enterocolitis (NEC). Cell Mol Gastroenterol Hepatol. 2018;5:549-568 pubmed publisher
	Carter E, Gopsill J, Gomm J, Jones J, Grose R. A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer. Breast Cancer Res. 2017;19:50 pubmed publisher
	Qu Y, Han B, Gao B, Bose S, Gong Y, Wawrowsky K, et al. Differentiation of Human Induced Pluripotent Stem Cells to Mammary-like Organoids. Stem Cell Reports. 2017;8:205-215 pubmed publisher
	Jung B, Obinata H, Galvani S, Mendelson K, Ding B, Skoura A, et al. Flow-regulated endothelial S1P receptor-1 signaling sustains vascular development. Dev Cell. 2012;23:600-10 pubmed publisher

image

image 1 :

Formalin-fixed, paraffin-embedded human breast carcinoma stained with ESA antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.

product information

Product Type :

Antibody

Product Name :

EpCAM Monoclonal Antibody (323/A3)

Catalog # :

MA5-12436

Quantity :

500 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 1-2 µg/mL, Radioimmune assays: Assay-dependent

Species :

Human, Mouse

Clone :

323/A3

Isotype :

IgG1

Storage :

4° C

Description :

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

Immunogen :

MCF-7 human breast cancer cells

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 1-2 µg/mL, Radioimmune assays: Assay-dependent

Aliases :

adenocarcinoma-associated antigen; CD326; cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; EPCAM; Ep-CAM; EpCAM1; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; gp40; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; Ly74; lymphocyte antigen 74; M1S2; M4S1; major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; Protein D5.7A; Tacsd1; Tacstd1; TROP1; Trop-1 protein; Tumor-associated calcium signal transducer 1

more info or order :

Invitrogen product webpage