This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD44 Monoclonal Antibody (5F12)
catalog :
MA5-12394
quantity :
500 µL
price :
US 410.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5F12
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, blocking or activating experiments
citations: 25
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; loading ...
Horvatinovich J, Grogan E, Norris M, Steinkasserer A, Lemos H, Mellor A, et al. Soluble CD83 Inhibits T Cell Activation by Binding to the TLR4/MD-2 Complex on CD14+ Monocytes. J Immunol. 2017;198:2286-2301 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 2e
Secundino I, Lizcano A, Roupé K, Wang X, Cole J, Olson J, et al. Host and pathogen hyaluronan signal through human siglec-9 to suppress neutrophil activation. J Mol Med (Berl). 2016;94:219-33 pubmed publisher
  • blocking or activating experiments; human
Hayashi T, Unno A, Baba M, Ohno T, Kitoh K, Takashima Y. CD44 mediated hyaluronan adhesion of Toxoplasma gondii-infected leukocytes. Parasitol Int. 2014;63:479-84 pubmed publisher
  • blocking or activating experiments; human; 20 ug/ml
Wu S, Chen C, Chang J, Fu Y, Wang C, Eswaramoorthy R, et al. Hyaluronan initiates chondrogenesis mainly via CD44 in human adipose-derived stem cells. J Appl Physiol (1985). 2013;114:1610-8 pubmed publisher
  • flow cytometry; human; 1:50
Zhao J, Gao Z, Mei H, Li Y, Wang Y. Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation. Am J Med Sci. 2011;341:460-8 pubmed publisher
  • blocking or activating experiments; human; 50 ug/ml
Hashizume M, Mihara M. High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1. Biochem Biophys Res Commun. 2010;403:184-9 pubmed publisher
  • flow cytometry; human
Rodgers A, Nair A, Binkley P, TEKMAL R, Schenken R. Inhibition of CD44 N- and O-linked glycosylation decreases endometrial cell lines attachment to peritoneal mesothelial cells. Fertil Steril. 2011;95:823-5 pubmed publisher
  • blocking or activating experiments; mouse; 5 ug/ml
Funayama K, Saito Kurimoto Y, Ebihara Y, Shimane M, Nomura H, Tsuji K, et al. Adhesion-mediated self-renewal abilities of Ph+ blastoma cells. Biochem Biophys Res Commun. 2010;396:193-8 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml
  • immunocytochemistry; human; 1:100
Funayama K, Shimane M, Nomura H, Asano S. An evidence for adhesion-mediated acquisition of acute myeloid leukemic stem cell-like immaturities. Biochem Biophys Res Commun. 2010;392:271-6 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human; 1:50
Yamawaki H, Hirohata S, Miyoshi T, Takahashi K, Ogawa H, Shinohata R, et al. Hyaluronan receptors involved in cytokine induction in monocytes. Glycobiology. 2009;19:83-92 pubmed publisher
  • flow cytometry; human
Pellizzaro C, Speranza A, Zorzet S, Crucil I, Sava G, Scarlata I, et al. Inhibition of human pancreatic cell line MIA PaCa2 proliferation by HA-But, a hyaluronic butyric ester: a preliminary report. Pancreas. 2008;36:e15-23 pubmed publisher
  • blocking or activating experiments; human; 20 ug/ml
Wang C, Lin Y, Chiang B, Lin Y, Hou S. High molecular weight hyaluronic acid down-regulates the gene expression of osteoarthritis-associated cytokines and enzymes in fibroblast-like synoviocytes from patients with early osteoarthritis. Osteoarthritis Cartilage. 2006;14:1237-47 pubmed
  • flow cytometry; human
He X, Li Y, Wang X, Guo X, Niu Y. [Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation]. Chin Med J (Engl). 2005;118:1728-34 pubmed
  • blocking or activating experiments; human
Tanaka M, Masuko Hongo K, Kato T, Nishioka K, Nakamura H. Suppressive effects of hyaluronan on MMP-1 and RANTES production from chondrocytes. Rheumatol Int. 2006;26:185-90 pubmed
Dorandish S, Williams A, Atali S, Sendo S, Price D, Thompson C, et al. Regulation of amyloid-β levels by matrix metalloproteinase-2/9 (MMP2/9) in the media of lung cancer cells. Sci Rep. 2021;11:9708 pubmed publisher
Dorandish S, Devos J, Clegg B, Price D, Muterspaugh R, Guthrie J, et al. Biochemical determinants of the IGFBP-3-hyaluronan interaction. FEBS Open Bio. 2020;10:1668-1684 pubmed publisher
Price D, Muterspaugh R, Clegg B, Williams A, Stephens A, Guthrie J, et al. IGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner. Sci Rep. 2020;10:5083 pubmed publisher
Ferreira S, Motwani M, Faull P, Seymour A, Yu T, Enayati M, et al. Bi-directional cell-pericellular matrix interactions direct stem cell fate. Nat Commun. 2018;9:4049 pubmed publisher
Ferreira S, Faull P, Seymour A, Yu T, Loaiza S, Auner H, et al. Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence. Biomaterials. 2018;176:13-23 pubmed publisher
Guo J, Hsu H, Tyan S, Li F, Shew J, Lee W, et al. Serglycin in tumor microenvironment promotes non-small cell lung cancer aggressiveness in a CD44-dependent manner. Oncogene. 2017;36:2457-2471 pubmed publisher
O Brien S, Chen L, Zhong W, Armellino D, Yu J, Loreth C, et al. Breast cancer cells respond differentially to modulation of TGFβ2 signaling after exposure to chemotherapy or hypoxia. Cancer Res. 2015;75:4605-16 pubmed publisher
Liang J, Jiang D, Jung Y, Xie T, Ingram J, Church T, et al. Role of hyaluronan and hyaluronan-binding proteins in human asthma. J Allergy Clin Immunol. 2011;128:403-411.e3 pubmed publisher
Coradini D, Zorzet S, Rossin R, Scarlata I, Pellizzaro C, Turrin C, et al. Inhibition of hepatocellular carcinomas in vitro and hepatic metastases in vivo in mice by the histone deacetylase inhibitor HA-But. Clin Cancer Res. 2004;10:4822-30 pubmed
Coradini D, Pellizzaro C, Abolafio G, Bosco M, Scarlata I, Cantoni S, et al. Hyaluronic-acid butyric esters as promising antineoplastic agents in human lung carcinoma: a preclinical study. Invest New Drugs. 2004;22:207-17 pubmed
Esnault S, Malter J. Hyaluronic acid or TNF-alpha plus fibronectin triggers granulocyte macrophage-colony-stimulating factor mRNA stabilization in eosinophils yet engages differential intracellular pathways and mRNA binding proteins. J Immunol. 2003;171:6780-7 pubmed
product information
Product Type :
Antibody
Product Name :
CD44 Monoclonal Antibody (5F12)
Catalog # :
MA5-12394
Quantity :
500 µL
Price :
US 410.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 10 µg/mL
Species :
Human
Clone :
5F12
Isotype :
IgG1
Storage :
4° C
Description :
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
Immunogen :
EBV transformed normal human B-lymphocytes
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 10 µg/mL
Aliases :
AU023126; AW121933; AW146109; Bovine CD44; CD44; CD44 antigen; CD44 antigen-like protein; CD44 molecule; CD44 molecule (Indian blood group); CD44 protein; CD44 variant; CD44A; CD44-like protein; CDW44; cell surface glycoprotein CD44; Cell surface glycoprotein CD44 (hyaluronate binding protein); cell-surface glycoprotein; chondroitin sulfate proteoglycan 8; CSPG8; ECMRIII; ECMR-III; Epican; extracellular matrix receptor III; extracellular matrix receptor-III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; HERMES; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; IN; LHR; Ly-24; lymphocyte antigen 24; lymphocyte surface antigen precursor CD44; MC56; MDU2; MDU3; METAA; MIC4; PGP I; Pgp1; PGP-1; PGPI; PGP-I; Phagocytic glycoprotein 1; phagocytic glycoprotein I; putative Bos taurus CD44; RHAMM; sCD 44; sCD44; soluble CD 44; soluble CD44
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA