This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Estrogen Receptor alpha Monoclonal Antibody (AER314)
catalog :
MA5-12328
quantity :
500 µL
price :
US 482.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AER314
reactivity :
human, mouse, rat, bovine, domestic rabbit
application :
western blot, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, blocking or activating experiments, western blot knockout validation
citations: 17
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; fig 4
Park W, Choi J, Hwang E, Lee J. Identification of a variant estrogen receptor lacking exon 4 and its coexpression with wild-type estrogen receptor in ovarian carcinomas. Clin Cancer Res. 1996;2:2029-35 pubmed
  • western blot; human; fig s8
Yoshimaru T, Komatsu M, Matsuo T, Chen Y, Murakami Y, Mizuguchi K, et al. Targeting BIG3-PHB2 interaction to overcome tamoxifen resistance in breast cancer cells. Nat Commun. 2013;4:2443 pubmed publisher
  • EMSA; human
Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed publisher
  • chromatin immunoprecipitation; human
Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 pubmed publisher
  • western blot; mouse
Milanesi L, Vasconsuelo A, de Boland A, Boland R. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue. Steroids. 2009;74:489-97 pubmed publisher
  • blocking or activating experiments; mouse
  • immunocytochemistry; mouse; 1:50
  • western blot; mouse; 1:400
Milanesi L, Russo de Boland A, Boland R. Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line. J Cell Biochem. 2008;104:1254-73 pubmed publisher
  • chromatin immunoprecipitation; mouse
Milne T, Martin M, Brock H, Slany R, Hess J. Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications. Cancer Res. 2005;65:11367-74 pubmed
Laganiere J, Deblois G, Giguere V. Nuclear receptor target gene discovery using high-throughput chromatin immunoprecipitation. Methods Enzymol. 2003;364:339-50 pubmed
Krieg A, Krieg S, Ahn B, Shapiro D. Interplay between estrogen response element sequence and ligands controls in vivo binding of estrogen receptor to regulated genes. J Biol Chem. 2004;279:5025-34 pubmed
Thomas P, Risinger K, Klinge C. Identification of estrogen receptor beta expression in Chinese hamster ovary (CHO) cells and comparison of estrogen-responsive gene transcription in cells adapted to serum-free media. J Steroid Biochem Mol Biol. 2003;86:41-55 pubmed
Kimmins S, Russell G, Lim H, Hall B, Maclaren L. The effects of estrogen, its antagonist ICI 182, 780, and interferon-tau on the expression of estrogen receptors and integrin alphaV beta 3 on cycle day 16 in bovine endometrium. Reprod Biol Endocrinol. 2003;1:38 pubmed
Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, et al. Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition. Biol Reprod. 2002;66:976-82 pubmed
Monje P, Zanello S, Holick M, Boland R. Differential cellular localization of estrogen receptor alpha in uterine and mammary cells. Mol Cell Endocrinol. 2001;181:117-29 pubmed
Lamb J, Ladha M, McMahon C, Sutherland R, Ewen M. Regulation of the functional interaction between cyclin D1 and the estrogen receptor. Mol Cell Biol. 2000;20:8667-75 pubmed
Feng W, Graumann K, Hahn R, Jungbauer A. Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography. J Chromatogr A. 1999;852:161-73 pubmed
McMahon C, Suthiphongchai T, DiRenzo J, Ewen M. P/CAF associates with cyclin D1 and potentiates its activation of the estrogen receptor. Proc Natl Acad Sci U S A. 1999;96:5382-7 pubmed
Biswas D, Averboukh L, Sheng S, Martin K, Ewaniuk D, Jawde T, et al. Classification of breast cancer cells on the basis of a functional assay for estrogen receptor. Mol Med. 1998;4:454-67 pubmed
product information
Product Type :
Antibody
Product Name :
Estrogen Receptor alpha Monoclonal Antibody (AER314)
Catalog # :
MA5-12328
Quantity :
500 µL
Price :
US 482.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Bovine, Human, Mouse, Rabbit, Rat
Applications :
Gel Shift: 1 mg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1:100-1:400
Species :
Bovine, Human, Mouse, Rabbit, Rat
Clone :
AER314
Isotype :
IgG1, lambda
Storage :
4° C
Description :
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Immunogen :
Purified, SDS-denatured calf uterus estrogen receptor (ER)
Format :
Liquid
Applications w/Dilutions :
Gel Shift: 1 mg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1:100-1:400
Aliases :
DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA