This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
SLC5A5 Monoclonal Antibody (FP5A)
catalog :
MA5-12308
quantity :
500 µL
price :
US 450.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FP5A
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:150; loading ...; fig 2
Lukianova N, Andriiv A, Chekhun V. Correlation of iodine symporter expression in highly and low malignant cell lines of human breast cancer differed in their sensitivity to doxorubicin. Exp Oncol. 2016;38:169-71 pubmed
  • western blot; human; 1:450; fig 2g
Samuel J, Singh N, Kanwar J, Krishnakumar S, Kanwar R. Upregulation of sodium iodide symporter (NIS) protein expression by an innate immunity component: Promising potential for targeting radiosensitive retinoblastoma. Exp Eye Res. 2015;139:108-14 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 6
Takeuchi Y, Inubushi M, Jin Y, Murai C, Tsuji A, Hata H, et al. Detailed assessment of gene activation levels by multiple hypoxia-responsive elements under various hypoxic conditions. Ann Nucl Med. 2014;28:1011-9 pubmed publisher
  • immunocytochemistry; human; 1:1000
  • immunohistochemistry; human
Pugliese M, Fortunati N, Germano A, Asioli S, Marano F, Palestini N, et al. Histone deacetylase inhibition affects sodium iodide symporter expression and induces 131I cytotoxicity in anaplastic thyroid cancer cells. Thyroid. 2013;23:838-46 pubmed publisher
  • immunohistochemistry; human
Lacoste C, Hervé J, Bou Nader M, Dos Santos A, Moniaux N, Valogne Y, et al. Iodide transporter NIS regulates cancer cell motility and invasiveness by interacting with the Rho guanine nucleotide exchange factor LARG. Cancer Res. 2012;72:5505-15 pubmed publisher
  • immunohistochemistry; human; 1:1000
  • western blot; human; 1:2000
Inubushi M, Jin Y, Murai C, Hata H, Kitagawa Y, Saga T. Single-photon emission computed tomography of spontaneous liver metastasis from orthotopically implanted human colon cancer cell line stably expressing human sodium/iodide symporter reporter gene. EJNMMI Res. 2012;2:46 pubmed publisher
  • western blot; human
Jin Y, Inubushi M, Masamoto K, Odaka K, Aoki I, Tsuji A, et al. Long-term effects of hepatocyte growth factor gene therapy in rat myocardial infarct model. Gene Ther. 2012;19:836-43 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Li C, Ho T, Kao C, Wu S, Liang J, Hsiang C. Conserved charged amino acid residues in the extracellular region of sodium/iodide symporter are critical for iodide transport activity. J Biomed Sci. 2010;17:89 pubmed publisher
  • western blot; human
Li W, Ain K. Human sodium-iodide symporter (hNIS) gene expression is inhibited by a trans-active transcriptional repressor, NIS-repressor, containing PARP-1 in thyroid cancer cells. Endocr Relat Cancer. 2010;17:383-98 pubmed publisher
  • immunocytochemistry; human; 1:1000
Fortunati N, Catalano M, Marano F, Mugoni V, Pugliese M, Bosco O, et al. The pan-DAC inhibitor LBH589 is a multi-functional agent in breast cancer cells: cytotoxic drug and inducer of sodium-iodide symporter (NIS). Breast Cancer Res Treat. 2010;124:667-75 pubmed publisher
  • immunohistochemistry; human; 1:200
Farnedi A, Eusebi L, Poli F, Foschini M. Immunohistochemical expression of the human sodium/iodide symporter distinguishes malignant from benign gastric lesions. Int J Surg Pathol. 2009;17:327-34 pubmed publisher
  • western blot; human
Peyrottes I, Navarro V, Ondo Mendez A, Marcellin D, Bellanger L, Marsault R, et al. Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers. Eur J Endocrinol. 2009;160:215-25 pubmed publisher
  • western blot; human
Jin Y, Chung H, Kang J, Lee Y, Kimm K, Kim Y, et al. Radioiodine gene therapy of hepatocellular carcinoma targeted human alpha fetoprotein. Cancer Biother Radiopharm. 2008;23:551-60 pubmed publisher
  • western blot; rat; 1:2000
Yeom C, Chung J, Kang J, Jeon Y, Kim K, Jin Y, et al. Visualization of hypoxia-inducible factor-1 transcriptional activation in C6 glioma using luciferase and sodium iodide symporter genes. J Nucl Med. 2008;49:1489-97 pubmed publisher
  • immunocytochemistry; human; 1:150
Montiel Equihua C, Martin Duque P, De la Vieja A, Quintanilla M, Burnet J, Vassaux G, et al. Targeting sodium/iodide symporter gene expression for estrogen-regulated imaging and therapy in breast cancer. Cancer Gene Ther. 2008;15:465-73 pubmed publisher
  • immunohistochemistry; human
Liu B, Hervé J, Bioulac Sage P, Valogne Y, Roux J, Yilmaz F, et al. Sodium iodide symporter is expressed at the preneoplastic stages of liver carcinogenesis and in human cholangiocarcinoma. Gastroenterology. 2007;132:1495-503 pubmed
  • western blot; human
Liang J, Chen C, Huang S, Ho T, Hsiang C, Ding H, et al. A novel loss-of-function deletion in sodium/iodide symporter gene in follicular thyroid adenoma. Cancer Lett. 2005;230:65-71 pubmed
  • immunohistochemistry; rat; 1:500
  • western blot; rat; 1:500
Lee K, Kim H, Paik J, Matsui T, Choe Y, Choi Y, et al. Accuracy of myocardial sodium/iodide symporter gene expression imaging with radioiodide: evaluation with a dual-gene adenovirus vector. J Nucl Med. 2005;46:652-7 pubmed
Liu Y, Liu C, Pan Y, Zhou J, Ju H, Zhang Y. Pyruvate carboxylase promotes malignant transformation of papillary thyroid carcinoma and reduces iodine uptake. Cell Death Discov. 2022;8:423 pubmed publisher
R xed os S xe1 nchez E, Gonz xe1 lez Zamora A, Gonsebatt Bonaparte M, Meza Mata E, Gonz xe1 lez Delgado M, Z xe1 mago Amaro A, et al. Regulation of the Tpo, Tg, Duox2, Pds, and Mct8 genes involved in the synthesis of thyroid hormones after subchronic exposure to sodium nitrate in female Wistar rats. Environ Toxicol. 2021;36:2380-2391 pubmed publisher
Rathod M, Kelkar M, Valvi S, Salve G, De A. FOXA1 Regulation Turns Benzamide HDACi Treatment Effect-Specific in BC, Promoting NIS Gene-Mediated Targeted Radioiodine Therapy. Mol Ther Oncolytics. 2020;19:93-104 pubmed publisher
Koodie L, Robertson M, Chandrashekar M, Ruth G, Dunning M, Bianco R, et al. Rodents Versus Pig Model for Assessing the Performance of Serotype Chimeric Ad5/3 Oncolytic Adenoviruses. Cancers (Basel). 2019;11: pubmed publisher
Wächter S, Wunderlich A, Greene B, Roth S, Elxnat M, Fellinger S, et al. Selumetinib Activity in Thyroid Cancer Cells: Modulation of Sodium Iodide Symporter and Associated miRNAs. Int J Mol Sci. 2018;19: pubmed publisher
. Abstracts of the Endocrine Society of Australia's Annual Scientific Meeting 2017. Clin Endocrinol (Oxf). 2018;89 Suppl 1:4-94 pubmed publisher
Marlow L, Rohl S, Miller J, Knauf J, Fagin J, Ryder M, et al. Methodology, Criteria, and Characterization of Patient-Matched Thyroid Cell Lines and Patient-Derived Tumor Xenografts. J Clin Endocrinol Metab. 2018;103:3169-3182 pubmed publisher
Wächter S, Damanakis A, Elxnat M, Roth S, Wunderlich A, Verburg F, et al. Epigenetic Modifications in Thyroid Cancer Cells Restore NIS and Radio-Iodine Uptake and Promote Cell Death. J Clin Med. 2018;7: pubmed publisher
Lee J, Min H, Lee S, Kwon H, Chung J. Relations Between Pathological Markers and Radioiodine Scan and (18)F-FDG PET/CT Findings in Papillary Thyroid Cancer Patients With Recurrent Cervical Nodal Metastases. Nucl Med Mol Imaging. 2015;49:127-34 pubmed publisher
Merron A, Peerlinck I, Martin Duque P, Burnet J, Quintanilla M, Mather S, et al. SPECT/CT imaging of oncolytic adenovirus propagation in tumours in vivo using the Na/I symporter as a reporter gene. Gene Ther. 2007;14:1731-8 pubmed
product information
Product Type :
Antibody
Product Name :
SLC5A5 Monoclonal Antibody (FP5A)
Catalog # :
MA5-12308
Quantity :
500 µL
Price :
US 450.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
Immunohistochemistry (Paraffin): 1:400-1:800, Western Blot: Assay-dependent
Species :
Human
Clone :
FP5A
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
Immunogen :
A synthetic peptide corresponding to aa625-643 of hNIS protein
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:400-1:800, Western Blot: Assay-dependent
Aliases :
Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-)-symporter; Na+/I- symporter; Nis; SLC5A5; sodium iodide symporter; Sodium/iodide cotransporter; sodium-iodide symporter; solute carrier family 5 (sodium iodide symporter), member 5; solute carrier family 5 (sodium/iodide cotransporter), member 5; solute carrier family 5 member 5; solute carrier family 5, member 5; TDH1
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA