product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD4 Monoclonal Antibody (4B12)
catalog :
MA5-12259
quantity :
500 µL
price :
US 461.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4B12
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 47
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; fig 4g
Liu X, Kong W, Peterson C, McGrail D, Hoang A, Zhang X, et al. PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma. Nat Commun. 2020;11:2135 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...
George J, Saito M, Tsuta K, Iwakawa R, Shiraishi K, Scheel A, et al. Genomic Amplification of CD274 (PD-L1) in Small-Cell Lung Cancer. Clin Cancer Res. 2017;23:1220-1226 pubmed publisher
  • immunohistochemistry - paraffin section; human
Kim W, Jung H, Nam S, Kim T, Heo D, Kim C, et al. Expression of programmed cell death ligand 1 (PD-L1) in advanced stage EBV-associated extranodal NK/T cell lymphoma is associated with better prognosis. Virchows Arch. 2016;469:581-590 pubmed
  • immunohistochemistry; mouse; fig 6b
Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1; fig 3
Banat G, Tretyn A, Pullamsetti S, Wilhelm J, Weigert A, Olesch C, et al. Immune and Inflammatory Cell Composition of Human Lung Cancer Stroma. PLoS ONE. 2015;10:e0139073 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 1
Patyka M, Malamud D, Weissman D, Abrams W, Kurago Z. Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study. PLoS ONE. 2015;10:e0132942 pubmed publisher
  • immunohistochemistry - paraffin section; human
Jeon Y, Kim J, Sung J, Han J, Ko Y. Epstein-Barr virus-positive nodal T/NK-cell lymphoma: an analysis of 15 cases with distinct clinicopathological features. Hum Pathol. 2015;46:981-90 pubmed publisher
  • immunohistochemistry; human
Däster S, Eppenberger Castori S, Hirt C, Zlobec I, Delko T, Nebiker C, et al. High frequency of CD8 positive lymphocyte infiltration correlates with lack of lymph node involvement in early rectal cancer. Dis Markers. 2014;2014:792183 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Azzimonti B, Zavattaro E, Provasi M, Vidali M, Conca A, Catalano E, et al. Intense Foxp3+ CD25+ regulatory T-cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8+/Foxp3+ CD25+ ratio. Br J Dermatol. 2015;172:64-73 pubmed publisher
  • immunohistochemistry; human
Koch Hansen L, Sevelsted Møller L, Rabjerg M, Larsen D, Hansen T, Klinge L, et al. Expression of T-cell KV1.3 potassium channel correlates with pro-inflammatory cytokines and disease activity in ulcerative colitis. J Crohns Colitis. 2014;8:1378-91 pubmed publisher
  • immunohistochemistry - paraffin section; human
Rizzo S, Basso C, Troost D, Aronica E, Frigo A, Driessen A, et al. T-cell-mediated inflammatory activity in the stellate ganglia of patients with ion-channel disease and severe ventricular arrhythmias. Circ Arrhythm Electrophysiol. 2014;7:224-9 pubmed publisher
  • immunohistochemistry; human; 1:100
Pohl M, Kawakami N, Kitic M, Bauer J, Martins R, Fischer M, et al. T cell-activation in neuromyelitis optica lesions plays a role in their formation. Acta Neuropathol Commun. 2013;1:85 pubmed publisher
  • immunohistochemistry; human; 1:100
Hammerstad S, Jahnsen F, Tauriainen S, Hyoty H, Paulsen T, Norheim I, et al. Immunological changes and increased expression of myxovirus resistance protein a in thyroid tissue of patients with recent onset and untreated Graves' disease. Thyroid. 2014;24:537-44 pubmed publisher
  • immunohistochemistry; human; 1:20
Descours B, Lambert Niclot S, Mory B, Samri A, Charlotte F, Peytavin G, et al. Direct quantification of cell-associated HIV DNA in isolated rectal and blood memory CD4 T cells revealed their similar and low infection levels in long-term treated HIV-infected patients. J Acquir Immune Defic Syndr. 2013;62:255-9 pubmed publisher
  • immunohistochemistry; human; 1:100
Hammerstad S, Jahnsen F, Tauriainen S, Hyoty H, Paulsen T, Norheim I, et al. Inflammation and increased myxovirus resistance protein A expression in thyroid tissue in the early stages of Hashimoto's thyroiditis. Thyroid. 2013;23:334-41 pubmed publisher
  • immunohistochemistry; human; 1:20
Zizzi A, Aspriello S, Ferrante L, Stramazzotti D, Colella G, Balercia P, et al. Immunohistochemical correlation between microvessel density and lymphoid infiltrate in radicular cysts. Oral Dis. 2013;19:92-9 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
  • immunohistochemistry; human; 1:100
Prabowo A, Anink J, Lammens M, Nellist M, van den Ouweland A, Adle Biassette H, et al. Fetal brain lesions in tuberous sclerosis complex: TORC1 activation and inflammation. Brain Pathol. 2013;23:45-59 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 3e
Jeon Y, Go H, Nam S, Keam B, Kim T, Jung K, et al. Expression of the promyelocytic leukemia zinc-finger in T-lymphoblastic lymphoma and leukemia has strong implications for their cellular origin and greater association with initial bone marrow involvement. Mod Pathol. 2012;25:1236-45 pubmed publisher
  • immunohistochemistry; human
Kim S, Jeong H, Woo O, Seo J, Kim A, Lee E, et al. Tumor-infiltrating lymphocytes, tumor characteristics, and recurrence in patients with early breast cancer. Am J Clin Oncol. 2013;36:224-31 pubmed publisher
  • immunohistochemistry; human; 1:10
West N, Milne K, Truong P, MacPherson N, Nelson B, Watson P. Tumor-infiltrating lymphocytes predict response to anthracycline-based chemotherapy in estrogen receptor-negative breast cancer. Breast Cancer Res. 2011;13:R126 pubmed publisher
  • immunohistochemistry; human; 1:20
Wu M, Kuo T, Ho H. Tumor-infiltrating lymphocytes contain a higher proportion of FOXP3(+) T lymphocytes in cervical cancer. J Formos Med Assoc. 2011;110:580-6 pubmed publisher
  • immunohistochemistry; human; 1:20; fig S5B
Ruffell B, Au A, Rugo H, Esserman L, Hwang E, Coussens L. Leukocyte composition of human breast cancer. Proc Natl Acad Sci U S A. 2012;109:2796-801 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:10; tbl 2
West N, Panet Raymond V, Truong P, Alexander C, Babinszky S, Milne K, et al. Intratumoral Immune Responses Can Distinguish New Primary and True Recurrence Types of Ipsilateral Breast Tumor Recurrences (IBTR). Breast Cancer (Auckl). 2011;5:105-15 pubmed publisher
  • immunohistochemistry; human; 1:100
Sta M, Sylva Steenland R, Casula M, de Jong J, Troost D, Aronica E, et al. Innate and adaptive immunity in amyotrophic lateral sclerosis: evidence of complement activation. Neurobiol Dis. 2011;42:211-20 pubmed publisher
  • immunohistochemistry; human; 1:20
Riezu Boj J, Larrea E, Aldabe R, Guembe L, Casares N, Galeano E, et al. Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection. J Hepatol. 2011;54:422-31 pubmed publisher
  • immunohistochemistry; human
Gordon S, Cervasi B, Odorizzi P, Silverman R, Aberra F, Ginsberg G, et al. Disruption of intestinal CD4+ T cell homeostasis is a key marker of systemic CD4+ T cell activation in HIV-infected individuals. J Immunol. 2010;185:5169-79 pubmed publisher
  • immunohistochemistry; human
Ferrara G, Chiarelli C, Simonetti S. B-cell lymphofollicular infiltrates in mycosis fungoides. Tumori. 2010;96:487-91 pubmed
  • immunohistochemistry; human; 1:5
El Shazly A, Arafa M, Roncarati P, Dortu E, Lefebvre P, Delvenne P. Characterization of hepatitis C virus-induced nasal mucosa remodelling. Histopathology. 2010;57:488-92 pubmed publisher
  • immunohistochemistry; mouse
Chen C, Yeh Y, Wu G, Huang Y, Lai W, Liu J, et al. Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging. Reproduction. 2010;140:105-12 pubmed publisher
  • immunohistochemistry; human; 1:20
Tao X, Xia J, Chen X, Wang H, Dai Y, Rhodus N, et al. FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity. Oral Dis. 2010;16:76-82 pubmed publisher
  • immunohistochemistry; human; 1:10; fig 1
Milne K, Barnes R, Girardin A, Mawer M, Nesslinger N, Ng A, et al. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer. PLoS ONE. 2008;3:e3409 pubmed publisher
  • immunohistochemistry; human
Estes J, Baker J, Brenchley J, Khoruts A, Barthold J, Bantle A, et al. Collagen deposition limits immune reconstitution in the gut. J Infect Dis. 2008;198:456-64 pubmed publisher
  • immunohistochemistry; human; 1:100
Aronica E, Boer K, Becker A, Redeker S, Spliet W, van Rijen P, et al. Gene expression profile analysis of epilepsy-associated gangliogliomas. Neuroscience. 2008;151:272-92 pubmed
  • immunohistochemistry; human
Keller T, van der Meer J, Teeling P, van der Sluijs K, Idu M, Rimmelzwaan G, et al. Selective expansion of influenza A virus-specific T cells in symptomatic human carotid artery atherosclerotic plaques. Stroke. 2008;39:174-9 pubmed
  • immunocytochemistry; human; 1:100
Boer K, Jansen F, Nellist M, Redeker S, van den Ouweland A, Spliet W, et al. Inflammatory processes in cortical tubers and subependymal giant cell tumors of tuberous sclerosis complex. Epilepsy Res. 2008;78:7-21 pubmed
  • immunohistochemistry; human; 1:20
Dong H, Elstrand M, Holth A, Silins I, Berner A, Trope C, et al. NK- and B-cell infiltration correlates with worse outcome in metastatic ovarian carcinoma. Am J Clin Pathol. 2006;125:451-8 pubmed
  • immunohistochemistry; human
Ruprecht C, Gattorno M, Ferlito F, Gregorio A, Martini A, Lanzavecchia A, et al. Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia. J Exp Med. 2005;201:1793-803 pubmed
  • immunohistochemistry; human
Gattorno M, Prigione I, Morandi F, Gregorio A, Chiesa S, Ferlito F, et al. Phenotypic and functional characterisation of CCR7+ and CCR7- CD4+ memory T cells homing to the joints in juvenile idiopathic arthritis. Arthritis Res Ther. 2005;7:R256-67 pubmed
Autio M, Leivonen S, Brück O, Mustjoki S, Mészáros Jørgensen J, Karjalainen Lindsberg M, et al. Immune cell constitution in the tumor microenvironment predicts the outcome in diffuse large B-cell lymphoma. Haematologica. 2021;106:718-729 pubmed publisher
Hawkshaw N, Pilkington S, Murphy S, Al Gazaq N, Farrar M, Watson R, et al. UV radiation recruits CD4+GATA3+ and CD8+GATA3+ T cells while altering the lipid microenvironment following inflammatory resolution in human skin in vivo. Clin Transl Immunology. 2020;9:e01104 pubmed publisher
Lennikov A, Saddala M, Mukwaya A, Tang S, Huang H. Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation. Front Immunol. 2019;10:1903 pubmed publisher
Saeed M, Mertens R, Handgretinger R, Efferth T. Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling. Int J Oncol. 2018;53:1721-1731 pubmed publisher
Ludwig B, Ludwig S, Steffen A, Knauf Y, Zimerman B, Heinke S, et al. Favorable outcome of experimental islet xenotransplantation without immunosuppression in a nonhuman primate model of diabetes. Proc Natl Acad Sci U S A. 2017;114:11745-11750 pubmed publisher
Shanmugasundaram U, Hilton J, Critchfield J, Greenblatt R, Giudice L, Averbach S, et al. Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium. Am J Reprod Immunol. 2016;76:137-48 pubmed publisher
Radnai B, Sturm E, Stančić A, Jandl K, Labocha S, Ferreirós N, et al. Eosinophils Contribute to Intestinal Inflammation via Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells, CRTH2, in Experimental Crohn's Disease. J Crohns Colitis. 2016;10:1087-95 pubmed publisher
Lemoine R, Pachlopnik Schmid J, Farin H, Bigorgne A, Debré M, Sepulveda F, et al. Immune deficiency-related enteropathy-lymphocytopenia-alopecia syndrome results from tetratricopeptide repeat domain 7A deficiency. J Allergy Clin Immunol. 2014;134:1354-1364.e6 pubmed publisher
Bunders M, van der Loos C, Klarenbeek P, van Hamme J, Boer K, Wilde J, et al. Memory CD4(+)CCR5(+) T cells are abundantly present in the gut of newborn infants to facilitate mother-to-child transmission of HIV-1. Blood. 2012;120:4383-90 pubmed publisher
image
image 1 :
Invitrogen MA5-12259 image 1
Formalin-fixed, paraffin-embedded human tonsil stained with CD4 antibody using peroxidase-conjugate and DAB chromogen. Note membrane staining of T cells.
product information
Product Type :
Antibody
Product Name :
CD4 Monoclonal Antibody (4B12)
Catalog # :
MA5-12259
Quantity :
500 µL
Price :
US 461.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1:2, Immunohistochemistry (Paraffin): 1:10-1:20, Western Blot: 1:10-1:100
Species :
Human
Clone :
4B12
Isotype :
IgG1, kappa
Storage :
4° C
Description :
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
Immunogen :
Stimulated human leukocytes
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1:2, Immunohistochemistry (Paraffin): 1:10-1:20, Western Blot: 1:10-1:100
Aliases :
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA