Pds1 Monoclonal Antibody (DCS-280) | Invitrogen MA5-12252 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Pds1 Monoclonal Antibody (DCS-280)

catalog :

MA5-12252

quantity :

500 µL

price :

US 413.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-280

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 8	Myatt S, Kongsema M, Man C, Kelly D, Gomes A, Khongkow P, et al. SUMOylation inhibits FOXM1 activity and delays mitotic transition. Oncogene. 2014;33:4316-29 pubmed publisher
western blot; human; 1:500	Caporali S, Alvino E, Levati L, Esposito A, Ciomei M, Brasca M, et al. Down-regulation of the PTTG1 proto-oncogene contributes to the melanoma suppressive effects of the cyclin-dependent kinase inhibitor PHA-848125. Biochem Pharmacol. 2012;84:598-611 pubmed publisher
western blot; human	Wu Y, Yen W, Lee T, Yih L. Heat shock protein inhibitors, 17-DMAG and KNK437, enhance arsenic trioxide-induced mitotic apoptosis. Toxicol Appl Pharmacol. 2009;236:231-8 pubmed publisher
western blot; mouse	Wishart T, Pemberton H, James S, McCabe C, Gillingwater T. Modified cell cycle status in a mouse model of altered neuronal vulnerability (slow Wallerian degeneration; Wlds). Genome Biol. 2008;9:R101 pubmed publisher
western blot; human	McNeely S, Taylor B, States J. Mitotic arrest-associated apoptosis induced by sodium arsenite in A375 melanoma cells is BUBR1-dependent. Toxicol Appl Pharmacol. 2008;231:61-7 pubmed publisher
western blot; human; 1:1000	Kim D, Franklyn J, Smith V, Stratford A, Pemberton H, Warfield A, et al. Securin induces genetic instability in colorectal cancer by inhibiting double-stranded DNA repair activity. Carcinogenesis. 2007;28:749-59 pubmed
western blot; mouse	Huang X, Hatcher R, York J, Zhang P. Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells. Mol Biol Cell. 2005;16:4725-32 pubmed
western blot; human	Huang X, Tran T, Zhang L, Hatcher R, Zhang P. DNA damage-induced mitotic catastrophe is mediated by the Chk1-dependent mitotic exit DNA damage checkpoint. Proc Natl Acad Sci U S A. 2005;102:1065-70 pubmed
	Repo H, Gurvits N, Löyttyniemi E, Nykanen M, Lintunen M, Karra H, et al. PTTG1-interacting protein (PTTG1IP/PBF) predicts breast cancer survival. BMC Cancer. 2017;17:705 pubmed publisher
	Hernando E, Nahle Z, Juan G, Diaz Rodriguez E, Alaminos M, Hemann M, et al. Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control. Nature. 2004;430:797-802 pubmed
	Michel L, Diaz Rodriguez E, Narayan G, Hernando E, Murty V, Benezra R. Complete loss of the tumor suppressor MAD2 causes premature cyclin B degradation and mitotic failure in human somatic cells. Proc Natl Acad Sci U S A. 2004;101:4459-64 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human tonsil stained with Pds1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lympocytes in germinal center.

product information

Product Type :

Antibody

Product Name :

Pds1 Monoclonal Antibody (DCS-280)

Catalog # :

MA5-12252

Quantity :

500 µL

Price :

US 413.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunohistochemistry (Paraffin): 1:100-200, Western Blot: 1-3 µg/mL

Species :

Human, Mouse

Clone :

DCS-280

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

Pds1 is an anaphase inhibitor and plays important role in DNA damage and spindle check point pathways. Pds1 inhibits sister chromatid separation by binding and inhibiting Esp1, cystein protease that causes cleavage of the cohesin Scc1 that binds the sister chromatids together. Pds1 is responsible for targeting of Esp1 to nucleus and its binding to the spindle. Degradation of Pds1 occurs shortly before anaphase, which liberates Esp1 and is a prerequisite for anaphase entry. Pds1 is targeted for degradation by ubiquitination mediated by cyclosome/anaphase-promoting complex (APC) functioning as a ubiquitin ligase. In response to DNA damage Chk1 phosphorylates Pds1 to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase.

Immunogen :

Human recombinant full length Pds1 protein

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:100-200, Western Blot: 1-3 µg/mL

Aliases :

AL033362; C11orf43; GRDF; Igf2; Igf-2; IGF-II; insulin like growth factor 2; insulin-like growth factor 2; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; insulin-like growth factor type 2; M6pr; Mpr; multiplication-stimulating polypeptide; Peg2; PP1446; PP9974; preptin; somatomedin A; Somatomedin-A; T3M-11-derived growth factor

more info or order :

Invitrogen product webpage