This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TIMP2 Monoclonal Antibody (3A4)
catalog :
MA5-12207
quantity :
500 µL
price :
US 460.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3A4
reactivity :
guinea pig, human, mouse, rat, bovine, domestic rabbit
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
Cid S, Eiró N, Gonzalez L, Béridze N, Vazquez J, Vizoso F. Expression and Clinical Significance of Metalloproteases and Their Inhibitors by Endothelial Cells From Invasive Breast Carcinomas. Clin Breast Cancer. 2016;16:e83-91 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 2
Nunia K, Urs A, Kumar P. Interplay Between MMP-9 and TIMP-2 Regulates Ameloblastoma Behavior and Tooth Morphogenesis. Appl Immunohistochem Mol Morphol. 2016;24:364-72 pubmed publisher
  • immunohistochemistry; human; 1:200
Kim G, Lee J, Choi Y, Lee K, Lee J, Nam J, et al. Expression of matrix metalloproteinases and their inhibitors in different immunohistochemical-based molecular subtypes of breast cancer. BMC Cancer. 2014;14:959 pubmed publisher
  • immunohistochemistry; human; 1:50
Suárez Roa M, Asbun Bojalil J, Ruiz Godoy L, Meneses García A. Immunoexpression of matrix metalloproteinases and their inhibitors in different areas of oral squamous cell carcinoma. Aust Dent J. 2012;57:300-7 pubmed publisher
  • immunohistochemistry; human; 1:50
Fernández Guinea O, Alvarez Cofiño A, Eiró N, Gonzalez L, del Casar J, Fernandez Garcia B, et al. Low microvascular density at the tumor center is related to the expression of metalloproteases and their inhibitors and with the occurrence of distant metastasis in breast carcinomas. Int J Clin Oncol. 2013;18:629-40 pubmed publisher
  • western blot; rat; 2 ug/ml
Liu C, Wang G, Chen G, Mu Y, Zhang L, Hu X, et al. Huangqi decoction inhibits apoptosis and fibrosis, but promotes Kupffer cell activation in dimethylnitrosamine-induced rat liver fibrosis. BMC Complement Altern Med. 2012;12:51 pubmed publisher
  • immunohistochemistry; human; 1:50
Gonzalez L, Eiró N, Gonzalez L, Andicoechea A, Barbón E, García Muñiz J, et al. Effect of the expression of matrix metalloproteases and their tissue inhibitors on survival of patients with resectable colorectal cancer. Dig Dis Sci. 2012;57:2063-71 pubmed publisher
  • immunohistochemistry; domestic rabbit
Song M, Shen H, Cui J, Zhou X, Zhong X, Peng C, et al. A novel model of intimal hyperplasia with graded hypoosmotic damage. Cardiovasc Pathol. 2012;21:490-8 pubmed publisher
  • immunohistochemistry; human; 4 ug/ml
Brun J, Cortez A, Lesieur B, Uzan S, Rouzier R, Darai E. Expression of MMP-2, -7, -9, MT1-MMP and TIMP-1 and -2 has no prognostic relevance in patients with advanced epithelial ovarian cancer. Oncol Rep. 2012;27:1049-57 pubmed publisher
  • immunohistochemistry; human
Dimo B, Ioannidis I, Karameris A, Vilaras G, Tzoumakari P, Nonni A, et al. Comparative study of the immunohistochemical expression of tissue inhibitors of metalloproteinases 1 and 2 between clearly invasive carcinomas and "in situ" trophoblast invasion. Med Oncol. 2012;29:2270-5 pubmed publisher
  • immunohistochemistry; human
Lee S, Park S, Kim S, Choi E. Expression of matrix metalloproteinases and their inhibitors in squamous cell carcinoma of the tonsil and their clinical significance. Clin Exp Otorhinolaryngol. 2011;4:88-94 pubmed publisher
  • immunohistochemistry; bovine; 1:100
Moreira L, de Carvalho E, Caldas Bussiere M. Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase. Vet Res Commun. 2011;35:261-9 pubmed publisher
  • immunohistochemistry; human; 1:200
Wu Y, Wang P, Tsai A, Yang S, Chen S. Semi-quantitative expression of tissue inhibitor of matrix metalloproteinase-2 in cancer of uterine cervix. J Surg Oncol. 2011;104:210-5 pubmed publisher
  • immunohistochemistry; human; 1:200
Wang P, Ko J, Yang S, Tsai H, Tee Y, Han C, et al. Significant relation of tissue inhibitor of matrix metalloproteinase-2 and its combination with matrix metalloproteinase-2 to survival of patients with cancer of uterine cervix. Reprod Sci. 2011;18:798-808 pubmed publisher
  • western blot; human; 1:200
Liu L, Sun L, Zhao P, Yao L, Jin H, Liang S, et al. Hypoxia promotes metastasis in human gastric cancer by up-regulating the 67-kDa laminin receptor. Cancer Sci. 2010;101:1653-60 pubmed publisher
  • immunohistochemistry; human; 1:50
Gonzalez L, Junquera S, del Casar J, Gonzalez L, Marín L, Gonzalez Reyes S, et al. Immunohistochemical study of matrix metalloproteinases and their inhibitors in pure and mixed invasive and in situ ductal carcinomas of the breast. Hum Pathol. 2010;41:980-9 pubmed publisher
  • immunohistochemistry; rat; 1:100
Ucar E, Borazan A, Semerci E, Binici D, Yaldiz M, Aysal A, et al. The effects of interferon alpha2b on chemically-induced peritoneal fibrosis and on peritoneal tissue MMP-2 and TIMP-2 levels in rats. J Int Med Res. 2010;38:187-94 pubmed
  • immunohistochemistry; human; 1:50
Escaff S, Fernandez J, Gonzalez L, Suarez A, Gonzalez Reyes S, Gonzalez J, et al. Study of matrix metalloproteinases and their inhibitors in prostate cancer. Br J Cancer. 2010;102:922-9 pubmed publisher
  • immunohistochemistry; human; 1:50
Gonzalez L, Gonzalez Reyes S, Junquera S, Marin L, Gonzalez L, del Casar J, et al. Expression of metalloproteases and their inhibitors by tumor and stromal cells in ductal carcinoma in situ of the breast and their relationship with microinvasive events. J Cancer Res Clin Oncol. 2010;136:1313-21 pubmed publisher
  • immunohistochemistry; rat; 1:200
Yilmaz B, Ozat M, Kilic S, Gungor T, Aksoy Y, Lordlar N, et al. Atorvastatin causes regression of endometriotic implants in a rat model. Reprod Biomed Online. 2010;20:291-9 pubmed publisher
  • immunohistochemistry; human; 1:50
del Casar J, Carreño G, Gonzalez L, Junquera S, Gonzalez Reyes S, Gonzalez J, et al. Expression of metalloproteases and their inhibitors in primary tumors and in local recurrences after mastectomy for breast cancer. J Cancer Res Clin Oncol. 2010;136:1049-58 pubmed publisher
  • immunohistochemistry; rat; 1:200
Yilmaz B, Sucak A, Kilic S, Aksakal O, Aksoy Y, Lortlar N, et al. Metformin regresses endometriotic implants in rats by improving implant levels of superoxide dismutase, vascular endothelial growth factor, tissue inhibitor of metalloproteinase-2, and matrix metalloproteinase-9. Am J Obstet Gynecol. 2010;202:368.e1-8 pubmed publisher
  • immunohistochemistry; human; 1:50
Nam E, Lee S, Im S, Kim D, Lee K, Sung S. Expression of cyclooxygenase-2 in human breast cancer: relationship with HER-2/neu and other clinicopathological prognostic factors. Cancer Res Treat. 2005;37:165-70 pubmed publisher
  • immunohistochemistry; human; 1:50
del Casar J, Gonzalez Reyes S, Gonzalez L, Gonzalez J, Junquera S, Bongera M, et al. Expression of metalloproteases and their inhibitors in different histological types of breast cancer. J Cancer Res Clin Oncol. 2010;136:811-9 pubmed publisher
  • immunohistochemistry; human; 1:50
del Casar J, Gonzalez L, Alvarez E, Junquera S, Marin L, Gonzalez L, et al. Comparative analysis and clinical value of the expression of metalloproteases and their inhibitors by intratumor stromal fibroblasts and those at the invasive front of breast carcinomas. Breast Cancer Res Treat. 2009;116:39-52 pubmed publisher
  • immunohistochemistry; mouse; 1:250
Zion O, Genin O, Kawada N, Yoshizato K, Roffe S, Nagler A, et al. Inhibition of transforming growth factor beta signaling by halofuginone as a modality for pancreas fibrosis prevention. Pancreas. 2009;38:427-35 pubmed publisher
  • immunohistochemistry; human; 4.0 ug/ml
Brun J, Galant C, Delvaux D, Lemoine P, Henriet P, Courtoy P, et al. Menstrual activity of matrix metalloproteinases is decreased in endometrium regenerating after thermal ablation. Hum Reprod. 2009;24:333-40 pubmed publisher
  • immunohistochemistry; human
Brun J, Cortez A, Commo F, Uzan S, Rouzier R, Darai E. Serous and mucinous ovarian tumors express different profiles of MMP-2, -7, -9, MT1-MMP, and TIMP-1 and -2. Int J Oncol. 2008;33:1239-46 pubmed
  • immunohistochemistry; human
Unsal D, Akyurek N, Uner A, Erpolat O, Han U, Akmansu M, et al. Gelatinase B expression as a prognostic factor in patients with stage II/III rectal carcinoma treated by postoperative adjuvant therapy. Am J Clin Oncol. 2008;31:55-63 pubmed publisher
  • western blot; rat; 1:1000
Yang M, Huang H, Li J, Huang W, Wang H. Connective tissue growth factor increases matrix metalloproteinase-2 and suppresses tissue inhibitor of matrix metalloproteinase-2 production by cultured renal interstitial fibroblasts. Wound Repair Regen. 2007;15:817-24 pubmed
  • immunohistochemistry; human; 1:50
Gonzalez L, Pidal I, Junquera S, Corte M, Vazquez J, Rodriguez J, et al. Overexpression of matrix metalloproteinases and their inhibitors in mononuclear inflammatory cells in breast cancer correlates with metastasis-relapse. Br J Cancer. 2007;97:957-63 pubmed
  • immunohistochemistry; domestic rabbit
Aupperle H, Garbade J, Schubert A, Barten M, Dhein S, Schoon H, et al. Effects of autologous stem cells on immunohistochemical patterns and gene expression of metalloproteinases and their tissue inhibitors in doxorubicin cardiomyopathy in a rabbit model. Vet Pathol. 2007;44:494-503 pubmed
  • immunohistochemistry; human; 1:50
Vizoso F, Gonzalez L, Corte M, Rodriguez J, Vazquez J, Lamelas M, et al. Study of matrix metalloproteinases and their inhibitors in breast cancer. Br J Cancer. 2007;96:903-11 pubmed
  • immunohistochemistry; human
Unsal Kilic D, Uner A, Akyurek N, Erpolat P, Dursun A, Pak Y. Matrix metalloproteinase-9 expression correlated with tumor response in patients with locally advanced rectal cancer undergoing preoperative chemoradiotherapy. Int J Radiat Oncol Biol Phys. 2007;67:196-203 pubmed
  • immunohistochemistry; human; 1:100
Rönkkö S, Rekonen P, Kaarniranta K, Puustjarvi T, Teräsvirta M, Uusitalo H. Matrix metalloproteinases and their inhibitors in the chamber angle of normal eyes and patients with primary open-angle glaucoma and exfoliation glaucoma. Graefes Arch Clin Exp Ophthalmol. 2007;245:697-704 pubmed
  • immunohistochemistry; human; 1:200
Zhang X, Bo X, Anderson P, Lieberman A, Zhang Y. Distribution and expression of tissue inhibitors of metalloproteinase in dorsal root entry zone and dorsal column after dorsal root injury. J Neurosci Res. 2006;84:278-90 pubmed
  • immunohistochemistry; human; 1:75
Masumoto K, de Rooij J, Suita S, Rottier R, Tibboel D, de Krijger R. The distribution of matrix metalloproteinases and tissue inhibitors of metalloproteinases in the lungs of congenital diaphragmatic hernia patients and age-matched controls. Histopathology. 2006;48:588-95 pubmed
  • immunohistochemistry; human; 1:400
Sier C, Zuidwijk K, Zijlmans H, Hanemaaijer R, Mulder Stapel A, Prins F, et al. EMMPRIN-induced MMP-2 activation cascade in human cervical squamous cell carcinoma. Int J Cancer. 2006;118:2991-8 pubmed
  • immunohistochemistry; human; 1:75
Masumoto K, de Rooij J, Suita S, Rottier R, Tibboel D, de Krijger R. Expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases during normal human pulmonary development. Histopathology. 2005;47:410-9 pubmed
Daraban Bocaneti F, Altamura G, Corteggio A, Tanase O, Dascalu M, Pașca S, et al. Expression of matrix metalloproteinases (MMPs)-2/-7/-9/-14 and tissue inhibitors of MMPs (TIMPs)-1/-2 in bovine cutaneous fibropapillomas associated with BPV-2 infection. Front Vet Sci. 2022;9:1063580 pubmed publisher
Johnson A, Zager R. Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI. J Am Soc Nephrol. 2018;29:2157-2167 pubmed publisher
Qian Y, Song J, Sun P, Yi R, Liu H, Feng X, et al. Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells. Molecules. 2018;23: pubmed publisher
Chlenski A, Liu S, Cohn S. The regulation of angiogenesis in neuroblastoma. Cancer Lett. 2003;197:47-52 pubmed
Gavin P, Crawford S, Shulman S, Garcia F, Rowley A. Systemic arterial expression of matrix metalloproteinases 2 and 9 in acute Kawasaki disease. Arterioscler Thromb Vasc Biol. 2003;23:576-81 pubmed
Hashimoto T, Wen G, Lawton M, Boudreau N, Bollen A, Yang G, et al. Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations. Stroke. 2003;34:925-31 pubmed
product information
Product Type :
Antibody
Product Name :
TIMP2 Monoclonal Antibody (3A4)
Catalog # :
MA5-12207
Quantity :
500 µL
Price :
US 460.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Bovine, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications :
Immunohistochemistry (Paraffin): 1:200, Western Blot: 1-2 µg/mL
Species :
Bovine, Guinea Pig, Human, Mouse, Rabbit, Rat
Clone :
3A4
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Immunogen :
A synthetic peptide from the N-terminal region of human TIMP-2 protein
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:200, Western Blot: 1-2 µg/mL
Aliases :
Collagenase inhibitor; CSC-21K; D11Bwg1104e; DDC8; etID32613.12; fa96h09; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2a; metalloproteinase inhibitor precursor; metalloproteinase inhibitor TIMP-2; metalloproteinase-2; similar to metalloproteinase inhibitor; testicular secretory protein Li 57; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP metallopeptidase inhibitor 2a; TIMP2; Timp-2; TIMP-2 protein; timp2a; tissue inhibitor of matrix metalloproteinase-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase 2 precursor; tissue inhibitor of metalloproteinase 2a; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinase-2 precursor; tissue inhibitor of metalloproteinases 2; tissue inhibitor of mettaloproteinase 2; wu:fa96h09; zgc:136726
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA