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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
VEGF Monoclonal Antibody (VG1)
catalog :
MA5-12184
quantity :
500 µL
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
VG1
reactivity :
human, mouse, rat, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 57
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; fig 3
Wang Y, Tadjuidje E, Pandey R, Stefater J, Smith L, Lang R, et al. The Eyes Absent Proteins in Developmental and Pathological Angiogenesis. Am J Pathol. 2016;186:568-78 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Gurzu S, Kádár Z, Sugimura H, Bara T, Hălmaciu I, Jung I. Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity. APMIS. 2015;123:223-33 pubmed publisher
  • immunohistochemistry; human; 1:100
Kostić J, Orlić D, Borović M, Beleslin B, Milašinović D, Dobrić M, et al. Coronary thrombi neovascularization in patients with ST-elevation myocardial infarction - clinical and angiographic implications. Thromb Res. 2014;134:1038-45 pubmed publisher
  • immunohistochemistry; human; 1:50
Lattanzio R, La Sorda R, Facciolo F, Sioletic S, Lauriola L, Martucci R, et al. Thymic epithelial tumors express vascular endothelial growth factors and their receptors as potential targets of antiangiogenic therapy: a tissue micro array-based multicenter study. Lung Cancer. 2014;85:191-6 pubmed publisher
  • western blot; mouse
Zhang Q, Zhang Z. Oxygen-induced retinopathy in mice with retinal photoreceptor cell degeneration. Life Sci. 2014;102:28-35 pubmed publisher
  • immunohistochemistry; human; 1:100
Abdelzaher E, El Deeb N, Gowil A, Yehya A. Biological and demographic profile of meningiomas in a cohort of Egyptian patients: impact on tumor recurrence. ScientificWorldJournal. 2013;2013:375139 pubmed publisher
  • immunohistochemistry; human
Tamaki M, Kamio T, Kameoka S, Kojimahara N, Nishikawa T. The relevance of the intrinsic subtype to the clinicopathological features and biomarkers in Japanese breast cancer patients. World J Surg Oncol. 2013;11:293 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; tbl 2
Qiu S, Wei X, Huang W, Wu M, Qin Y, Li Y, et al. Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma. Sci Rep. 2013;3:2529 pubmed publisher
  • immunohistochemistry; human; 1:25
Gurzu S, Ciortea D, Munteanu T, Kezdi Zaharia I, Jung I. Mesenchymal-to-endothelial transition in Kaposi sarcoma: a histogenetic hypothesis based on a case series and literature review. PLoS ONE. 2013;8:e71530 pubmed publisher
  • immunohistochemistry; domestic rabbit
Karimian E, Tamm C, Chagin A, Samuelsson K, Kjartansdóttir K, Ohlsson C, et al. Resveratrol treatment delays growth plate fusion and improves bone growth in female rabbits. PLoS ONE. 2013;8:e67859 pubmed publisher
  • immunohistochemistry; human; 1:100
Feng C, Ding G, Song N, Li X, Wu Z, Jiang H, et al. Paraneoplastic hormones: parathyroid hormone-related protein (PTHrP) and erythropoietin (EPO) are related to vascular endothelial growth factor (VEGF) expression in clear cell renal cell carcinoma. Tumour Biol. 2013;34:3471-6 pubmed publisher
  • immunocytochemistry; human; 1:50
Radziwon Balicka A, Moncada de la Rosa C, Zielnik B, Doroszko A, Jurasz P. Temporal and pharmacological characterization of angiostatin release and generation by human platelets: implications for endothelial cell migration. PLoS ONE. 2013;8:e59281 pubmed publisher
  • western blot; mouse
Figueras A, Arbos M, Quiles M, Vinals F, Germà J, Capella G. The impact of KRAS mutations on VEGF-A production and tumour vascular network. BMC Cancer. 2013;13:125 pubmed publisher
  • immunohistochemistry; rat; 1:100
Micili S, Goker A, Sayin O, Akokay P, Ergur B. The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats. J Mol Histol. 2013;44:339-45 pubmed publisher
  • immunohistochemistry; human
Melinte R, Jung I, Georgescu L, Gurzu S. VEGF and CD31 expression in arthritic synovium and cartilage of human knee joints. Rom J Morphol Embryol. 2012;53:911-5 pubmed
  • immunohistochemistry; human
Bohn O, de León E, Lezama O, Rios Luna N, Sanchez Sosa S, Llombart Bosch A. Pulmonary artery sarcoma with angiosarcoma phenotype mimicking pleomorphic malignant fibrous histiocytoma: a case report. Diagn Pathol. 2012;7:154 pubmed publisher
  • immunohistochemistry; rat
Ozer H, Boztosun A, Açmaz G, Atilgan R, Akkar O, Koşar M. The efficacy of bevacizumab, sorafenib, and retinoic acid on rat endometriosis model. Reprod Sci. 2013;20:26-32 pubmed publisher
  • immunohistochemistry; human
Kasai M, Ichimura T, Kawamura N, Sumikura T, Matsuda M, Asano C, et al. Prediction of the shrinking rate of uterine leiomyoma nodules using needle biopsy specimens. Fertil Steril. 2012;98:440-3 pubmed publisher
  • immunohistochemistry - paraffin section; rat
Boztosun A, Piçnak A, Kosar M, Gulturk S, Cetin A. Effects of methylene blue, pentoxyphylline and enoxaparin on postoperative adhesion formation and markers of angiogenesis in a rat uterine horn model. Clin Exp Obstet Gynecol. 2012;39:89-95 pubmed
  • immunohistochemistry; human; 1:100
Ding G, Feng C, Song N, Fang Z, Xia G, Jiang H, et al. Paraneoplastic symptoms: cachexia, polycythemia, and hypercalcemia are, respectively, related to vascular endothelial growth factor (VEGF) expression in renal clear cell carcinoma. Urol Oncol. 2013;31:1820-5 pubmed publisher
  • immunohistochemistry; human; 1:20
Moshkin O, Rotondo F, Scheithauer B, Soares M, Coire C, Smyth H, et al. Bronchial carcinoid tumors metastatic to the sella turcica and review of the literature. Pituitary. 2012;15:160-5 pubmed publisher
  • immunocytochemistry; human
Nylander M, Osman A, Ramstrom S, Aklint E, Larsson A, Lindahl T. The role of thrombin receptors PAR1 and PAR4 for PAI-1 storage, synthesis and secretion by human platelets. Thromb Res. 2012;129:e51-8 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Xu Z, Zhao F, Lin F, Chen J, Huang Y. Lipoxin A4 inhibits the development of endometriosis in mice: the role of anti-inflammation and anti-angiogenesis. Am J Reprod Immunol. 2012;67:491-7 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Jung Y, Joo K, Seong D, Choi Y, Kong D, Kim Y, et al. Identification of prognostic biomarkers for glioblastomas using protein expression profiling. Int J Oncol. 2012;40:1122-32 pubmed publisher
  • immunohistochemistry; human
Tolunay I, Tunaoglu S, Akyurek N, Halid V, Olgunturk R, Kula S. Serum and pulmonary vascular endothelial growth factor/receptors and haemodynamic measurements in cyanotic congenital heart disease with decreased pulmonary blood flow. Cardiol Young. 2011;21:608-15 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Yadav V, Prasad S, Sung B, Gelovani J, Guha S, Krishnan S, et al. Boswellic acid inhibits growth and metastasis of human colorectal cancer in orthotopic mouse model by downregulating inflammatory, proliferative, invasive and angiogenic biomarkers. Int J Cancer. 2012;130:2176-84 pubmed publisher
  • immunohistochemistry; human; 1:50
Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, et al. Immunohistochemical study of the angiogenetic network of VEGF, HIF1?, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer. Pathol Oncol Res. 2012;18:33-41 pubmed publisher
  • immunohistochemistry; human
Karaca Z, Tanriverdi F, Unluhizarci K, Ozturk F, Gokahmetoglu S, Elbuken G, et al. VEGFR1 expression is related to lymph node metastasis and serum VEGF may be a marker of progression in the follow-up of patients with differentiated thyroid carcinoma. Eur J Endocrinol. 2011;164:277-84 pubmed publisher
  • immunohistochemistry; human
Azamfirei L, Gurzu S, Solomon R, Copotoiu R, Copotoiu S, Jung I, et al. Vascular endothelial growth factor: a possible mediator of endothelial activation in acute respiratory distress syndrome. Minerva Anestesiol. 2010;76:609-16 pubmed
  • immunohistochemistry; rat; 1:50
Saylan A, Arioz D, Koken T, Dilek H, Saylan F, Yilmazer M. Prevention of ovarian hyperstimulation syndrome in a rat model: efficacy comparison between cabergoline and meloxicam. Acta Obstet Gynecol Scand. 2010;89:692-9 pubmed publisher
  • western blot; mouse
Zhang H, Schin M, Saha J, Burke K, Holzman L, Filipiak W, et al. Podocyte-specific overexpression of GLUT1 surprisingly reduces mesangial matrix expansion in diabetic nephropathy in mice. Am J Physiol Renal Physiol. 2010;299:F91-8 pubmed publisher
  • immunohistochemistry; human; 1:100
Bircan A, Bircan S, Kapucuoglu N, Songur N, Ozturk O, Akkaya A. Maspin, VEGF and p53 expression in small biopsies of primary advanced lung cancer and relationship with clinicopathologic parameters. Pathol Oncol Res. 2010;16:553-61 pubmed publisher
  • immunohistochemistry; human; 1:100
Sanci M, Dikis C, Inan S, Turkoz E, Dicle N, Ispahi C. Immunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma. Acta Histochem. 2011;113:317-25 pubmed publisher
  • immunohistochemistry; human
Yang S. Bevacizumab and breast cancer: current therapeutic progress and future perspectives. Expert Rev Anticancer Ther. 2009;9:1715-25 pubmed publisher
  • immunohistochemistry; human
van der Loos C, Meijer Jorna L, Broekmans M, Ploegmakers H, Teeling P, de Boer O, et al. Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels. J Histochem Cytochem. 2010;58:109-18 pubmed publisher
  • immunohistochemistry; human
Deniz H, Karakok M, Yagci F, Guldur M. Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas. Int Urol Nephrol. 2010;42:103-7 pubmed publisher
  • immunohistochemistry; human; 1:50
Jung I, Gurzu S, Raica M, Cimpean A, Szentirmay Z. The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis. Rom J Morphol Embryol. 2009;50:239-43 pubmed
  • immunohistochemistry; human; 1:50
Carpenter P, Chen W, Mendez A, McLaren C, Su M. Angiogenesis in the progression of breast ductal proliferations. Int J Surg Pathol. 2011;19:335-41 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 1
Dawson M, Opat S, Taouk Y, Donovan M, Zammit M, Monaghan K, et al. Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma. Clin Cancer Res. 2009;15:714-22 pubmed publisher
  • immunohistochemistry; human; 1:100
Kim H, Kim W, Park S, Jung C, Choi H, Lee H, et al. Molecular biomarkers for advanced renal cell carcinoma: implications for prognosis and therapy. Urol Oncol. 2010;28:157-63 pubmed publisher
  • immunohistochemistry; human; 1:100
Schneider B, Wang M, Radovich M, Sledge G, Badve S, Thor A, et al. Association of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 genetic polymorphisms with outcome in a trial of paclitaxel compared with paclitaxel plus bevacizumab in advanced breast cancer: ECOG 2100. J Clin Oncol. 2008;26:4672-8 pubmed publisher
  • immunohistochemistry; human
Gurzu S, Jung I, Prantner I, Ember I, Pávai Z, Mezei T. The expression of cytoskeleton regulatory protein Mena in colorectal lesions. Rom J Morphol Embryol. 2008;49:345-9 pubmed
  • immunohistochemistry; human
Sarioglu S, Celik A, Ersen A, Uçer I, Saglam F, Camsari T, et al. Vascular endothelial growth factor expression and vascularity in renal allograft biopsies. Transplant Proc. 2008;40:178-80 pubmed publisher
  • immunohistochemistry; human; 1:250
Timotheadou E, Skarlos D, Samantas E, Papadopoulos S, Murray S, Skrickova J, et al. Evaluation of the prognostic role of a panel of biomarkers in stage IB-IIIA non-small cell lung cancer patients. Anticancer Res. 2007;27:4481-9 pubmed
  • immunohistochemistry; human; 1:300
Ozbudak I, Karaveli S, Simsek T, Erdogan G, Pestereli E. Neoangiogenesis and expression of hypoxia-inducible factor 1alpha, vascular endothelial growth factor, and glucose transporter-1 in endometrioid type endometrium adenocarcinomas. Gynecol Oncol. 2008;108:603-8 pubmed publisher
  • immunohistochemistry; human
Cabuk D, Basaran G, Celikel C, Dane F, Yumuk P, Iyikesici M, et al. Vascular endothelial growth factor, hypoxia-inducible factor 1 alpha and CD34 expressions in early-stage gastric tumors: relationship with pathological factors and prognostic impact on survival. Oncology. 2007;72:111-7 pubmed
  • immunohistochemistry; human; 1:25
Bangoura G, Liu Z, Qian Q, Jiang C, Yang G, Jing S. Prognostic significance of HIF-2alpha/EPAS1 expression in hepatocellular carcinoma. World J Gastroenterol. 2007;13:3176-82 pubmed
  • immunohistochemistry; rat
Yülek F, Or M, Ozogul C, Isik A, Ari N, Stefek M, et al. Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress. Arch Med Res. 2007;38:503-11 pubmed
  • immunohistochemistry; human; 1:250
Phuoc N, Ehara H, Gotoh T, Nakano M, Yokoi S, Deguchi T, et al. Immunohistochemical analysis with multiple antibodies in search of prognostic markers for clear cell renal cell carcinoma. Urology. 2007;69:843-8 pubmed
  • immunohistochemistry; human
Bolat F, Kayaselcuk F, Nursal T, Yagmurdur M, Bal N, Demirhan B. Microvessel density, VEGF expression, and tumor-associated macrophages in breast tumors: correlations with prognostic parameters. J Exp Clin Cancer Res. 2006;25:365-72 pubmed
  • immunohistochemistry; rat; 1:50
Canda A, Mungan M, Yilmaz O, Yorukoglu K, Tuzel E, Kirkali Z. Effects of finasteride on the vascular surface density, number of microvessels and vascular endothelial growth factor expression of the rat prostate. Int Urol Nephrol. 2006;38:275-80 pubmed
  • immunohistochemistry; human
Tzankov A, Heiss S, Ebner S, Sterlacci W, Schaefer G, Augustin F, et al. Angiogenesis in nodal B cell lymphomas: a high throughput study. J Clin Pathol. 2007;60:476-82 pubmed
  • western blot; human; 1000
Haider D, Bucek R, Giurgea A, Maurer G, Glogar H, Minar E, et al. PGE1 analog alprostadil induces VEGF and eNOS expression in endothelial cells. Am J Physiol Heart Circ Physiol. 2005;289:H2066-72 pubmed
Charan M, Das S, Mishra S, Chatterjee N, Varikuti S, Kaul K, et al. Macrophage migration inhibitory factor inhibition as a novel therapeutic approach against triple-negative breast cancer. Cell Death Dis. 2020;11:774 pubmed publisher
Ibbett P, Goverdhan S, Pipi E, Chouhan J, Keeling E, Angus E, et al. A lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophy. Sci Rep. 2019;9:7475 pubmed publisher
Laçin N, İzol B, Tuncer M, Gokalp Ozkorkmaz E, Deveci B, Deveci E. Effects of formaldehyde on VEGF, MMP2, and Osteonectin levels in periodontal membrane and alveolar bone in the rats. Folia Morphol (Warsz). 2018;: pubmed publisher
Görük N, Deveci E. Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase1. Acta Cir Bras. 2018;33:629-640 pubmed publisher
image
image 1 :
Invitrogen MA5-12184 image 1
image 2 :
Invitrogen MA5-12184 image 2
Formalin-fixed, paraffin-embedded human angiosarcoma stained with VEGF antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
product information
Product Type :
Antibody
Product Name :
VEGF Monoclonal Antibody (VG1)
Catalog # :
MA5-12184
Quantity :
500 µL
Price :
US 399.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
Immunocytochemistry: 1:40-1:200, Immunohistochemistry (Paraffin): 1:100-1:200, Western Blot: 1-2 µg/mL
Species :
Human
Clone :
VG1
Isotype :
IgG1, kappa
Storage :
4° C
Description :
VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.
Immunogen :
Recombinant VEGF189 protein
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:40-1:200, Immunohistochemistry (Paraffin): 1:100-1:200, Western Blot: 1-2 µg/mL
Aliases :
angiogenic factor; etID309976.16; eVEGF120; eVEGF164; human VEGF121 homolog; MGC70609; MVCD1; ovine vascular endothelial growth factor; RP1-261G23.1; unnamed protein product; VAS; vascular endothelial growth factor; vascular endothelial growth factor (VEGF) precursor; vascular endothelial growth factor 121 isoform; vascular endothelial growth factor 164; vascular endothelial growth factor 165b; vascular endothelial growth factor 182; vascular endothelial growth factor 182 isoform; vascular endothelial growth factor 188; vascular endothelial growth factor 188 isoform; vascular endothelial growth factor 189; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular endothelial growth factor Aa; Vascular endothelial growth factor A-A; vascular endothelial growth factor isoform 165; vascular permeability factor; Vasculotropin; VEGF; VEGF1; VEGF164; VEGF182; VEGF188; vegfa; VEGF-A; VEGFA165b; vegfaa; VEGF-A-A; VEGFMGC70609; VPF; VPF ant; wu:fj82c06
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA