Cytokeratin HMW Monoclonal Antibody (34BetaE12) | Invitrogen MA5-12135 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cytokeratin HMW Monoclonal Antibody (34BetaE12)

catalog :

MA5-12135

quantity :

500 µL

price :

US 445.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

34betaE12

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:100; tbl 2	Hsieh M, Wang H, Lee Y, Ko J, Chang Y. Reevaluation of MAML2 fusion-negative mucoepidermoid carcinoma: a subgroup being actually hyalinizing clear cell carcinoma of the salivary gland with EWSR1 translocation. Hum Pathol. 2017;61:9-18 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Sajin M, Luchian M, Hodorogea PrisÄƒcaru A, Dumitru A, PÄƒtraÅŸcu O, Costache D, et al. Trichilemmal carcinoma - a rare cutaneous malignancy: report of two cases. Rom J Morphol Embryol. 2014;55:687-91 pubmed
immunohistochemistry; human	Stefanovic D, Stefanovic M, Nikin Z. Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent. Biotech Histochem. 2013;88:329-35 pubmed publisher
immunohistochemistry; human; 1:50	Marian C, Boilă A, Soanca D, Malau M, Podeanu D, Resetkova E, et al. Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature. APMIS. 2013;121:272-9 pubmed publisher
immunohistochemistry; human	Yang S, Sun R, Liang J, Zhou Z, Zhou J, Rui J. Sinonasal teratocarcinosarcoma: a clinical and pathological analysis. Int J Surg Pathol. 2013;21:37-43 pubmed publisher
immunohistochemistry; human; 1:30	Bolzoni Villaret A, Barbieri D, Peretti G, Schreiber A, Fisogni S, Lonardi S, et al. Angiogenesis and lymphangiogenesis in early-stage laryngeal carcinoma: Prognostic implications. Head Neck. 2013;35:1132-7 pubmed publisher
immunohistochemistry; human; 1:100	Lin J, Lu C, Lin W, Wu Y, Huang Y, Shih F, et al. A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma. J Clin Neurosci. 2012;19:836-43 pubmed publisher
immunohistochemistry; human; 1:100	Tao Y, Chai D, Ma L, Zhang T, Feng Z, Cheng Z, et al. Identification of distinct gene expression profiles between esophageal squamous cell carcinoma and adjacent normal epithelial tissues. Tohoku J Exp Med. 2012;226:301-11 pubmed
immunohistochemistry; human; 1:100	Lehmusvaara S, Erkkilä T, Urbanucci A, Waltering K, Seppälä J, Larjo A, et al. Chemical castration and anti-androgens induce differential gene expression in prostate cancer. J Pathol. 2012;227:336-45 pubmed publisher
immunohistochemistry; human; 1:100	Lin J, Wu Y, Chang I. The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution. Indian J Pathol Microbiol. 2011;54:683-7 pubmed publisher
immunohistochemistry; human; 1:100	Tolonen T, Kujala P, Laurila M, Tirkkonen M, Ilvesaro J, Tuominen V, et al. Routine dual-color immunostaining with a 3-antibody cocktail improves the detection of small cancers in prostate needle biopsies. Hum Pathol. 2011;42:1635-42 pubmed publisher
immunohistochemistry; human; 1:100	Chen H, Wu Y, Lin Y, Lin J. Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution. J Neurooncol. 2011;103:551-9 pubmed publisher
immunohistochemistry; human; 1:100	Lin J, Ho J, Lin Y, Wu Y. Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution. J Neurooncol. 2010;100:465-73 pubmed publisher
immunohistochemistry; human; 1:50	Midi A, Tecimer T, Bozkurt S, Ozkan N. Differences in the structural features of atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma. Indian J Urol. 2008;24:169-77 pubmed
	Jung M, Kowalczyk K, Hankins R, Bandi G, Kallakury B, Carrasquilla M, et al. Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients. Cancer Res Commun. 2022;2:1617-1625 pubmed publisher
	Kupczyk P, Rykala M, Serek P, Pawlak A, Slowikowski B, Holysz M, et al. The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin. J Vet Intern Med. 2022;36:1508-1524 pubmed publisher
	Gornalusse G, Valdez R, Fenkart G, Vojtech L, Fleming L, Pandey U, et al. Mechanisms of Endogenous HIV-1 Reactivation by Endocervical Epithelial Cells. J Virol. 2020;94: pubmed publisher
	Wang W, Bergh A, Damber J. Chronic inflammation in benign prostate hyperplasia is associated with focal upregulation of cyclooxygenase-2, Bcl-2, and cell proliferation in the glandular epithelium. Prostate. 2004;61:60-72 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human breast cancer stained with Keratin HMW antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

product information

Product Type :

Antibody

Product Name :

Cytokeratin HMW Monoclonal Antibody (34BetaE12)

Catalog # :

MA5-12135

Quantity :

500 µL

Price :

US 445.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:50-1:100, Western Blot: 1:1,000-1:10,000

Species :

Human

Clone :

34BetaE12

Isotype :

IgG1

Storage :

4° C

Description :

Keratins are heteropolymeric structural proteins that form the intermediate filament. Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of cytokeratins – the low weight, acidic type I and the high weight, basic type II. Cytokeratins are usually found in pairs – one type I and one type II. KRT1 regulates the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase I (RACK1). In complex with C1QBP it is a high affinity receptor for kininogen-1/HMWK. KRT1 is expressed in the spinous and granular layers of the epidermis with family member KRT10. Mutations affecting the gene result in bullous congenital ichthyosiform erythroderma. KRT5 is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations affecting the gene result in epidermolysis bullosa simplex diseases. KRT10 plays a role in the establishment of the epidermal barrier on plantar skin. It also acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB – thus it plays a role in nasal colonization. Mutations affecting the gene result in epidermolytic hyperkeratosis. KRT14’s nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Mutations affecting the gene result in epidermolysis bullosa simplex.

Immunogen :

Solubilized keratin extracted from human stratum corneum

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:50-1:100, Western Blot: 1:1,000-1:10,000

Aliases :

58 kda cytokeratin; 67 kDa cytokeratin; BCIE; BIE; CK1; CK-1; CK10; CK-10; CK14; CK-14; CK-2P; CK5; CK-5; cytokeratin 1; cytokeratin 10; cytokeratin 14; cytokeratin 2; cytokeratin-1; Cytokeratin-10; Cytokeratin-14; cytokeratin-2P; Cytokeratin-5; DDD; DDD1; EBS2; EBS3; EBS4; EHK; EHK1; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; EPPK; hair alpha protein; HUMCYT2A; K1; K10; K14; K2P; K5; K76; keratin 1; keratin 1, type II; keratin 10; keratin 10, type I; keratin 14; keratin 14, type I; keratin 2p; keratin 5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 76; keratin 76, type II; keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 oral; keratin, type II cytoskeletal 5; Keratin-1; Keratin-10; keratin-14; Keratin-5; Keratin-76; KPP; KRT1; KRT10; KRT14; KRT1A; KRT2B; KRT2P; KRT5; KRT5A; KRT76; KRTA; NEPPK; NFJ; Type-II keratin Kb1; type-II keratin Kb5; Type-II keratin Kb9

more info or order :

Invitrogen product webpage