XPF Monoclonal Antibody (219) | Invitrogen MA5-12054 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

XPF Monoclonal Antibody (219)

catalog :

MA5-12054

quantity :

500 µL

price :

US 456

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

219

reactivity :

hamsters, human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; fig 8	Fu H, Martin M, Regairaz M, Huang L, You Y, Lin C, et al. The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage. Nat Commun. 2015;6:6746 pubmed publisher
immunohistochemistry; human; 1:3000; fig s3	Egloff A, Lee J, Langer C, Quon H, Vaezi A, Grandis J, et al. Phase II study of cetuximab in combination with cisplatin and radiation in unresectable, locally advanced head and neck squamous cell carcinoma: Eastern cooperative oncology group trial E3303. Clin Cancer Res. 2014;20:5041-51 pubmed publisher
immunocytochemistry; human	Godon C, Mourgues S, Nonnekens J, Mourcet A, Coin F, Vermeulen W, et al. Generation of DNA single-strand displacement by compromised nucleotide excision repair. EMBO J. 2012;31:3550-63 pubmed publisher
western blot; human; 1:1000	DeLoia J, Bhagwat N, Darcy K, Strange M, Tian C, Nuttall K, et al. Comparison of ERCC1/XPF genetic variation, mRNA and protein levels in women with advanced stage ovarian cancer treated with intraperitoneal platinum. Gynecol Oncol. 2012;126:448-54 pubmed publisher
western blot; hamsters	Fisher L, Bessho M, Wakasugi M, Matsunaga T, Bessho T. Role of interaction of XPF with RPA in nucleotide excision repair. J Mol Biol. 2011;413:337-46 pubmed publisher
immunocytochemistry; human; 1:300	Rageul J, Frémin C, Ezan F, Baffet G, Langouet S. The knock-down of ERCC1 but not of XPF causes multinucleation. DNA Repair (Amst). 2011;10:978-90 pubmed publisher
immunoprecipitation; human	Kim Y, Lach F, Desetty R, Hanenberg H, Auerbach A, Smogorzewska A. Mutations of the SLX4 gene in Fanconi anemia. Nat Genet. 2011;43:142-6 pubmed publisher
western blot; human	Zhang Y, Regairaz M, Seiler J, Agama K, Doroshow J, Pommier Y. Poly(ADP-ribose) polymerase and XPF-ERCC1 participate in distinct pathways for the repair of topoisomerase I-induced DNA damage in mammalian cells. Nucleic Acids Res. 2011;39:3607-20 pubmed publisher
western blot; human	Arora S, Kothandapani A, Tillison K, Kalman Maltese V, Patrick S. Downregulation of XPF-ERCC1 enhances cisplatin efficacy in cancer cells. DNA Repair (Amst). 2010;9:745-53 pubmed publisher
immunoprecipitation; human; 1:1000 immunocytochemistry; human; 1:1000 western blot; human	Bhagwat N, Roginskaya V, Acquafondata M, Dhir R, Wood R, Niedernhofer L. Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue. Cancer Res. 2009;69:6831-8 pubmed publisher
immunoprecipitation; human	Wu Y, Mitchell T, Zhu X. Human XPF controls TRF2 and telomere length maintenance through distinctive mechanisms. Mech Ageing Dev. 2008;129:602-10 pubmed publisher
western blot; human; 1:200	Rubio D, Garcia S, Paz M, de la Cueva T, Lopez Fernandez L, Lloyd A, et al. Molecular characterization of spontaneous mesenchymal stem cell transformation. PLoS ONE. 2008;3:e1398 pubmed publisher
western blot; human	Fisher L, Bessho M, Bessho T. Processing of a psoralen DNA interstrand cross-link by XPF-ERCC1 complex in vitro. J Biol Chem. 2008;283:1275-81 pubmed
immunocytochemistry; human western blot; human	Wu Y, Zacal N, Rainbow A, Zhu X. XPF with mutations in its conserved nuclease domain is defective in DNA repair but functions in TRF2-mediated telomere shortening. DNA Repair (Amst). 2007;6:157-66 pubmed
	Panier S, Maric M, Hewitt G, Mason Osann E, Gali H, Dai A, et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell. 2019;76:27-43.e11 pubmed publisher
	Stoepker C, Hain K, Schuster B, Hilhorst Hofstee Y, Rooimans M, Steltenpool J, et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet. 2011;43:138-41 pubmed publisher
	Ahmad A, Enzlin J, Bhagwat N, Wijgers N, Raams A, Appledoorn E, et al. Mislocalization of XPF-ERCC1 nuclease contributes to reduced DNA repair in XP-F patients. PLoS Genet. 2010;6:e1000871 pubmed publisher

product information

Product Type :

Antibody

Product Name :

XPF Monoclonal Antibody (219)

Catalog # :

MA5-12054

Quantity :

500 µL

Price :

US 456

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human

Clone :

219

Storage :

4° C

Description :

The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.

Immunogen :

Recombinant human XPF protein

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

AI606920; DNA excision repair protein ERCC-4; DNA repair endonuclease subunit; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; ERCC excision repair 4, endonuclease catalytic subunit; ERCC11; ERCC4; Ercc4 protein-like; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; FANCQ; RAD1; RGD1560340; xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F; XFEPS; XPF