CHK2 Monoclonal Antibody (2CHK01 (DCS-293)) | Invitrogen MA5-11940 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CHK2 Monoclonal Antibody (2CHK01 (DCS-293))

catalog :

MA5-11940

quantity :

500 µL

price :

US 498

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2CHK01 (DCS-293)

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1500	Stephan H, Concannon C, Kremmer E, Carty M, Nasheuer H. Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res. 2009;37:6028-41 pubmed publisher
western blot; human; 1:1000	Jiang H, Wu J, He C, Yang W, Li H. Tumor suppressor protein C53 antagonizes checkpoint kinases to promote cyclin-dependent kinase 1 activation. Cell Res. 2009;19:458-68 pubmed publisher
	Tsvetkov L, Xu X, Li J, Stern D. Polo-like kinase 1 and Chk2 interact and co-localize to centrosomes and the midbody. J Biol Chem. 2003;278:8468-75 pubmed
	Tort F, Hernandez S, Bea S, Martinez A, Esteller M, Herman J, et al. CHK2-decreased protein expression and infrequent genetic alterations mainly occur in aggressive types of non-Hodgkin lymphomas. Blood. 2002;100:4602-8 pubmed

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product information

Product Type :

Antibody

Product Name :

CHK2 Monoclonal Antibody (2CHK01 (DCS-293))

Catalog # :

MA5-11940

Quantity :

500 µL

Pricing :

US 498

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 µg/mg protein Lysate, Western Blot: 1-3 µg/mL

Species :

Human

Clone :

2CHK01 (DCS-293)

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Checkpoint kinase 2 (Chk2) is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radition, and phosphorylate Thr68 and other sites in the amino-terminal region of Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphates, preventing the entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

Immunogen :

Recombinant Chk2 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 µg/mg protein Lysate, Western Blot: 1-3 µg/mL

Aliases :

CDS1; cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; checkpoint-like protein CHK2; Chek2; Chk2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; del9; EC 2.7.11.1; hCds1; HuCds 1; HuCds1; kinase Chk2; LFS2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; PP1425; protein kinase Chk2; Rad53; Rad53 homolog; RP11-436C9.1; Serine/threonine protein kinase Chk2; serine/threonine-protein kinase Chk2

more info or order :

Invitrogen product webpage