CD47 Monoclonal Antibody (B6H12.2) | Invitrogen MA5-11895 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD47 Monoclonal Antibody (B6H12.2)

catalog :

MA5-11895

quantity :

500 uL

price :

US 429.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B6H12.2

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 5c western blot; human; loading ...; fig 6b	Wang R, Geng J, Sheng W, Liu X, Jiang M, Zhen Y. The ionophore antibiotic gramicidin A inhibits pancreatic cancer stem cells associated with CD47 down-regulation. Cancer Cell Int. 2019;19:145 pubmed publisher
blocking or activating experiments; human western blot; human	Bergström S, Bergdahl E, Sundqvist K. A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation. Immunology. 2013;140:441-55 pubmed publisher
blocking or activating experiments; human	Ali N, Gaughan A, Orosz C, Baran C, McMaken S, Wang Y, et al. Latency associated peptide has in vitro and in vivo immune effects independent of TGF-beta1. PLoS ONE. 2008;3:e1914 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Isenberg J, Romeo M, Abu Asab M, Tsokos M, Oldenborg A, Pappan L, et al. Increasing survival of ischemic tissue by targeting CD47. Circ Res. 2007;100:712-20 pubmed
blocking or activating experiments; human; 2 ug/ml	Wandersee N, Punzalan R, Rettig M, Kennedy M, Pajewski N, Sabina R, et al. Erythrocyte adhesion is modified by alterations in cellular tonicity and volume. Br J Haematol. 2005;131:366-77 pubmed
	Li K, Huo Q, Dimmitt N, Qu G, Bao J, Pandya P, et al. Osteosarcoma-enriched transcripts paradoxically generate osteosarcoma-suppressing extracellular proteins. elife. 2023;12: pubmed publisher
	Meier L, Faragher J, Osinski V, Auger J, VOELLER R, Marath A, et al. CD47 Promotes Autoimmune Valvular Carditis by Impairing Macrophage Efferocytosis and Enhancing Cytokine Production. J Immunol. 2022;208:2643-2651 pubmed publisher
	Lin A, Razzokov J, Verswyvel H, Privat Maldonado A, De Backer J, Yusupov M, et al. Oxidation of Innate Immune Checkpoint CD47 on Cancer Cells with Non-Thermal Plasma. Cancers (Basel). 2021;13: pubmed publisher
	Mair B, Aldridge P, Atwal R, Philpott D, Zhang M, Masud S, et al. High-throughput genome-wide phenotypic screening via immunomagnetic cell sorting. Nat Biomed Eng. 2019;3:796-805 pubmed publisher
	Jain S, Van Scoyk A, Morgan E, Matthews A, Stevenson K, Newton G, et al. Targeted inhibition of CD47-SIRPα requires Fc-FcγR interactions to maximize activity in T-cell lymphomas. Blood. 2019;134:1430-1440 pubmed publisher
	Pitale D, Gendalur N, Descoteaux A, Shaha C. Leishmania donovani Induces Autophagy in Human Blood-Derived Neutrophils. J Immunol. 2019;202:1163-1175 pubmed publisher
	Sch xfc rch C, Forster S, Br xfc hl F, Yang S, Felley Bosco E, Hewer E. The "don't eat me" signal CD47 is a novel diagnostic biomarker and potential therapeutic target for diffuse malignant mesothelioma. Oncoimmunology. 2017;7:e1373235 pubmed publisher
	Kojima Y, Volkmer J, McKenna K, Civelek M, Lusis A, Miller C, et al. CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis. Nature. 2016;536:86-90 pubmed
	Congote L, Temmel N. The C-terminal 26-residue peptide of serpin A1 stimulates proliferation of breast and liver cancer cells: role of protein kinase C and CD47. FEBS Lett. 2004;576:343-7 pubmed
	Wandersee N, Olson S, Holzhauer S, Hoffmann R, Barker J, Hillery C. Increased erythrocyte adhesion in mice and humans with hereditary spherocytosis and hereditary elliptocytosis. Blood. 2004;103:710-6 pubmed

product information

Product Type :

Antibody

Product Name :

CD47 Monoclonal Antibody (B6H12.2)

Catalog # :

MA5-11895

Quantity :

500 uL

Price :

US 429.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 0.5 ug/test, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1:10-1:100

Species :

Human

Clone :

B6H12.2

Isotype :

IgG1, kappa

Storage :

4 C

Description :

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Immunogen :

Intact CD47 purified from placenta

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 ug/test, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1:10-1:100

Aliases :

9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen/integrin-associated protein; CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

more info or order :

Invitrogen product webpage