Involucrin Monoclonal Antibody (SY5) | Invitrogen MA5-11803 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Involucrin Monoclonal Antibody (SY5)

catalog :

MA5-11803

quantity :

500 µL

price :

US 472

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SY5

reactivity :

gorilla , human, mouse, rat, dogs, pigs , domestic sheep

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 60

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; fig 5d western blot; human; fig 7d	Bruce J, To K, Lui V, Chung G, Chan Y, Tsang C, et al. Whole-genome profiling of nasopharyngeal carcinoma reveals viral-host co-operation in inflammatory NF-κB activation and immune escape. Nat Commun. 2021;12:4193 pubmed publisher
immunohistochemistry; human; 1:100; loading ...; fig 3h western blot; human; 1:100; loading ...	Dabelsteen S, Pallesen E, Marinova I, Nielsen M, Adamopoulou M, Rømer T, et al. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Dev Cell. 2020;54:669-684.e7 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3c western blot; mouse; 1:100; loading ...; fig 4a western blot; human; 1:100; loading ...; fig 4b	Jeong H, Lim K, Goldenring J, Nam K. Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice. Biomol Ther (Seoul). 2019;27:553-561 pubmed publisher
western blot; human; 1:1000; loading ...; fig s1c	Zhang Z, Zi Z, Lee E, Zhao J, Contreras D, South A, et al. Differential glucose requirement in skin homeostasis and injury identifies a therapeutic target for psoriasis. Nat Med. 2018;24:617-627 pubmed publisher
immunohistochemistry; human; 500 ug/ml; tbl 2	Rigden H, Alias A, Havelock T, O Donnell R, Djukanovic R, Davies D, et al. Squamous Metaplasia Is Increased in the Bronchial Epithelium of Smokers with Chronic Obstructive Pulmonary Disease. PLoS ONE. 2016;11:e0156009 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig 4	Suzuki D, Sahu R, Leu N, Senoo M. The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells. Development. 2015;142:282-90 pubmed publisher
immunohistochemistry; human	Mainzer C, Barrichello C, Debret R, Remoue N, Sigaudo Roussel D, Sommer P. Insulin-transferrin-selenium as an alternative to foetal serum for epidermal equivalents. Int J Cosmet Sci. 2014;36:427-35 pubmed publisher
immunohistochemistry; human	Mitchell D, Israr M, Alam S, Dinello D, Kishel J, Jia R, et al. HIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium. HIV Med. 2014;15:196-202 pubmed publisher
immunocytochemistry; human	Maruyama H, Ishitsuka Y, Fujisawa Y, Furuta J, Sekido M, Kawachi Y. B-Myb enhances proliferation and suppresses differentiation of keratinocytes in three-dimensional cell culture. Arch Dermatol Res. 2014;306:375-84 pubmed publisher
immunohistochemistry; human; fig s2	Pontiggia L, Biedermann T, Böttcher Haberzeth S, Oliveira C, Braziulis E, Klar A, et al. De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells. J Invest Dermatol. 2014;134:1735-1742 pubmed publisher
western blot; human; fig 3	Wilson V. Growth and differentiation of HaCaT keratinocytes. Methods Mol Biol. 2014;1195:33-41 pubmed publisher
immunocytochemistry; human	Bulysheva A, Bowlin G, Petrova S, Yeudall W. Enhanced chemoresistance of squamous carcinoma cells grown in 3D cryogenic electrospun scaffolds. Biomed Mater. 2013;8:055009 pubmed publisher
immunohistochemistry; mouse	Hughes M, Jiang T, Lin S, Leung Y, Kobielak K, Widelitz R, et al. Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis. J Invest Dermatol. 2014;134:24-32 pubmed publisher
immunohistochemistry; human; 1:100	Pontiggia L, Klar A, Böttcher Haberzeth S, Biedermann T, Meuli M, Reichmann E. Optimizing in vitro culture conditions leads to a significantly shorter production time of human dermo-epidermal skin substitutes. Pediatr Surg Int. 2013;29:249-56 pubmed publisher
immunocytochemistry; human; 1:150	Fatimah S, Tan G, Chua K, Tan A, Nur Azurah A, Hayati A. Effects of keratinocyte growth factor on skin epithelial differentiation of human amnion epithelial cells. Burns. 2013;39:905-15 pubmed publisher
immunohistochemistry; human; 1:100	Watarai A, Amoh Y, Maejima H, Hamada Y, Katsuoka K. Nestin expression is increased in the suprabasal epidermal layer in psoriasis vulgaris. Acta Derm Venereol. 2013;93:39-43 pubmed publisher
immunohistochemistry; mouse; 1:200	Li J, Jiang T, Hughes M, Wu P, Yu J, Widelitz R, et al. Progressive alopecia reveals decreasing stem cell activation probability during aging of mice with epidermal deletion of DNA methyltransferase 1. J Invest Dermatol. 2012;132:2681-90 pubmed publisher
immunohistochemistry; rat; 2 ug/ml	Wato K, Hara T, Yamana K, Nakao H, Inagi T, Terada K. An insight into the role of barrier related skin proteins. Int J Pharm. 2012;427:293-8 pubmed publisher
immunohistochemistry; human	Mitchell D, Israr M, Alam S, Kishel J, Dinello D, Meyers C. Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium. HIV Med. 2012;13:276-90 pubmed publisher
immunocytochemistry; human; 1:100	Bulysheva A, Bowlin G, Klingelhutz A, Yeudall W. Low-temperature electrospun silk scaffold for in vitro mucosal modeling. J Biomed Mater Res A. 2012;100:757-67 pubmed publisher
western blot; human	Yamazaki S, Uchiumi A, Katagata Y. Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells. Int J Mol Med. 2012;29:165-8 pubmed publisher
immunohistochemistry; human; 1:500	Molina Ruiz A, Del Carmen Fariña M, Carrasco L, Santonja C, Rodriguez Peralto J, Torrelo A, et al. Saurian papulosis: a new clinicopathological entity. J Am Acad Dermatol. 2013;68:e17-22 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Kirschner N, Haftek M, Niessen C, Behne M, Furuse M, Moll I, et al. CD44 regulates tight-junction assembly and barrier function. J Invest Dermatol. 2011;131:932-43 pubmed publisher
western blot; human	Cheung P, Yip Y, Tsao S, Ching Y, Cheung A. Id-1 induces cell invasiveness in immortalized epithelial cells by regulating cadherin switching and Rho GTPases. J Cell Biochem. 2011;112:157-68 pubmed publisher
immunocytochemistry; rat; 1:100	Bonfanti P, Claudinot S, Amici A, Farley A, Blackburn C, Barrandon Y. Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells. Nature. 2010;466:978-82 pubmed publisher
immunohistochemistry; human; fig 4	Qi H, Zheng X, Yuan X, Pflugfelder S, Li D. Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium. J Cell Physiol. 2010;225:180-5 pubmed publisher
immunocytochemistry; human; 1:100 immunohistochemistry; human; 1:100	Biedermann T, Pontiggia L, Böttcher Haberzeth S, Tharakan S, Braziulis E, Schiestl C, et al. Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 2010;130:1996-2009 pubmed publisher
immunohistochemistry; human; 1:100	Tharakan S, Pontiggia L, Biedermann T, Böttcher Haberzeth S, Schiestl C, Reichmann E, et al. Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells. Pediatr Surg Int. 2010;26:71-7 pubmed publisher
immunohistochemistry; human	Metallo C, Azarin S, Moses L, Ji L, de Pablo J, Palecek S. Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs. Tissue Eng Part A. 2010;16:213-23 pubmed publisher
western blot; human	Warters R, Packard A, Kramer G, Gaffney D, Moos P. Differential gene expression in primary human skin keratinocytes and fibroblasts in response to ionizing radiation. Radiat Res. 2009;172:82-95 pubmed publisher
western blot; human; 1:100	Beckstead B, Tung J, Liang K, Tavakkol Z, Usui M, Olerud J, et al. Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model. J Biomed Mater Res A. 2009;91:436-46 pubmed publisher
flow cytometry; human	Gröger S, Michel J, Meyle J. Establishment and characterization of immortalized human gingival keratinocyte cell lines. J Periodontal Res. 2008;43:604-14 pubmed publisher
immunocytochemistry; human immunohistochemistry; human western blot; human	Araya J, Cambier S, Markovics J, Wolters P, Jablons D, Hill A, et al. Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients. J Clin Invest. 2007;117:3551-62 pubmed
immunocytochemistry; domestic sheep	Islam M, Zhou H. Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus. Eur J Dermatol. 2007;17:302-8 pubmed
immunohistochemistry; mouse western blot; mouse	Senoo M, Pinto F, Crum C, McKeon F. p63 Is essential for the proliferative potential of stem cells in stratified epithelia. Cell. 2007;129:523-36 pubmed
immunohistochemistry - frozen section; human; fig 2	Peltonen S, Riehokainen J, Pummi K, Peltonen J. Tight junction components occludin, ZO-1, and claudin-1, -4 and -5 in active and healing psoriasis. Br J Dermatol. 2007;156:466-72 pubmed
immunohistochemistry; human	Hachiya A, Sriwiriyanont P, Patel A, Saito N, Ohuchi A, Kitahara T, et al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther. 2007;14:648-56 pubmed
immunocytochemistry; human; 1:200	Hayashi N, Kido J, Kido R, Wada C, Kataoka M, Shinohara Y, et al. Regulation of calprotectin expression by interleukin-1alpha and transforming growth factor-beta in human gingival keratinocytes. J Periodontal Res. 2007;42:1-7 pubmed
immunocytochemistry; human; 1:40	Chen Z, Mok H, Pflugfelder S, Li D, Barry M. Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors. Exp Eye Res. 2006;83:798-806 pubmed
western blot; rat; 1:100	Beckstead B, Santosa D, Giachelli C. Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation. J Biomed Mater Res A. 2006;79:94-103 pubmed
immunocytochemistry; human; 1:40	Chen Z, Evans W, Pflugfelder S, Li D. Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells. Stem Cells. 2006;24:1265-73 pubmed
immunocytochemistry; human; 1:40 immunohistochemistry; human; 1:40	Li D, Chen Z, Song X, de Paiva C, Kim H, Pflugfelder S. Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness. Exp Eye Res. 2005;80:581-90 pubmed
western blot; human	Hasskarl J, Velupillai P, Piboonniyom S, Grace M, Munger K. Long-term maintenance of human keratinocytes in vitro. J Invest Dermatol. 2005;124:475-8 pubmed
immunohistochemistry; human; 1:100	Sanmano B, Mizoguchi M, Suga Y, Ikeda S, Ogawa H. Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite. J Dermatol Sci. 2005;37:29-39 pubmed
	Ana Choi S, Ko J, Yu V, Ning L, Lung M. Differentiation-related zinc finger protein 750 suppresses cell growth in esophageal squamous cell carcinoma. Oncol Lett. 2021;22:513 pubmed publisher
	Goričan L, Gole B, Potocnik U. Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening. Cells. 2020;9: pubmed publisher
	Li Y, Song Y, Zhu L, Wang X, Richers B, Leung D, et al. Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1. J Immunol Res. 2020;2020:5084682 pubmed publisher
	Terlecki Zaniewicz L, Pils V, Bobbili M, Lämmermann I, Perrotta I, Grillenberger T, et al. Extracellular vesicles in human skin: cross-talk from senescent fibroblasts to keratinocytes by miRNAs. J Invest Dermatol. 2019;: pubmed publisher
	Anderson E, Sastalla I, Earland N, Mahnaz M, Moore I, Otaizo Carrasquero F, et al. Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632. PLoS ONE. 2018;13:e0198862 pubmed publisher
	Zoschke C, Ulrich M, Sochorová M, Wolff C, Vavrova K, Ma N, et al. The barrier function of organotypic non-melanoma skin cancer models. J Control Release. 2016;233:10-8 pubmed publisher
	Neumann C, Bigliardi Qi M, Widmann C, Bigliardi P. The Î´-opioid receptor affects epidermal homeostasis via ERK-dependent inhibition of transcription factor POU2F3. J Invest Dermatol. 2015;135:471-480 pubmed publisher
	Mizoguchi M, Suga Y, Sanmano B, Ikeda S, Ogawa H. Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis. J Dermatol Sci. 2004;35:199-206 pubmed
	Uzgare A, Isaacs J. Enhanced redundancy in Akt and mitogen-activated protein kinase-induced survival of malignant versus normal prostate epithelial cells. Cancer Res. 2004;64:6190-9 pubmed
	Chen Z, de Paiva C, Luo L, Kretzer F, Pflugfelder S, Li D. Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells. 2004;22:355-66 pubmed
	Hale L, Buckley R, Puck J, Patel D. Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency. Clin Immunol. 2004;110:63-70 pubmed
	Hale L, Markert M. Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus. J Immunol. 2004;172:617-24 pubmed
	D Silva N, Mitra R, Zhang Z, Kurnit D, Babcock C, Polverini P, et al. Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes. J Cell Physiol. 2003;196:532-40 pubmed
	Hinterhuber G, Marquardt Y, Diem E, Rappersberger K, Wolff K, Foedinger D. Organotypic keratinocyte coculture using normal human serum: an immunomorphological study at light and electron microscopic levels. Exp Dermatol. 2002;11:413-20 pubmed
	Asai Y, Ohyama Y, Gen K, Ogawa T. Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2. Infect Immun. 2001;69:7387-95 pubmed
	Kee S, Steinert P. Microtubule disruption in keratinocytes induces cell-cell adhesion through activation of endogenous E-cadherin. Mol Biol Cell. 2001;12:1983-93 pubmed

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product information

Product Type :

Antibody

Product Name :

Involucrin Monoclonal Antibody (SY5)

Catalog # :

MA5-11803

Quantity :

500 µL

Pricing :

US 472

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Canine, Human, Non-human primate, Porcine, Rat

Applications :

ELISA: Assay-dependent, Immunohistochemistry (Paraffin): 1:100-200, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Canine, Human, Non-human primate, Porcine, Rat

Clone :

SY5

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

Immunogen :

Human keratinocytes' involucrin

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunohistochemistry (Paraffin): 1:100-200, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

1110019C06Rik; involucrin; IVL

more info or order :

Invitrogen product webpage