Involucrin Monoclonal Antibody (SY5), Biotin | Invitrogen MA5-11800 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Involucrin Monoclonal Antibody (SY5), Biotin

catalog :

MA5-11800

quantity :

500µL

price :

US 375.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

SY5

reactivity :

human, mouse, rat, dog, pig, sheep

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 37

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Metallo C, Azarin S, Moses L, Ji L, de Pablo J, Palecek S. Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs. Tissue Eng Part A. 2010;16:213-23 pubmed publisher
immunocytochemistry; human; 1:40 immunohistochemistry; human; 1:40	Li D, Chen Z, Song X, de Paiva C, Kim H, Pflugfelder S. Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness. Exp Eye Res. 2005;80:581-90 pubmed
flow cytometry; human	Gröger S, Michel J, Meyle J. Establishment and characterization of immortalized human gingival keratinocyte cell lines. J Periodontal Res. 2008;43:604-14 pubmed publisher
western blot; human	Hasskarl J, Velupillai P, Piboonniyom S, Grace M, Munger K. Long-term maintenance of human keratinocytes in vitro. J Invest Dermatol. 2005;124:475-8 pubmed
immunohistochemistry; human; 1:100	Sanmano B, Mizoguchi M, Suga Y, Ikeda S, Ogawa H. Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite. J Dermatol Sci. 2005;37:29-39 pubmed
immunocytochemistry; human; 1:100 immunohistochemistry; human; 1:100	Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, et al. Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 2010;130:1996-2009 pubmed publisher
immunohistochemistry; human	Mainzer C, Barrichello C, Debret R, Remoue N, Sigaudo-Roussel D, Sommer P. Insulin-transferrin-selenium as an alternative to foetal serum for epidermal equivalents. Int J Cosmet Sci. 2014;36:427-35 pubmed publisher
immunocytochemistry; rat; 1:100	Bonfanti P, Claudinot S, Amici A, Farley A, Blackburn C, Barrandon Y. Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells. Nature. 2010;466:978-82 pubmed publisher
immunohistochemistry; human; 1:500	Molina-Ruiz A, Del Carmen Fariña M, Carrasco L, Santonja C, Rodriguez-Peralto J, Torrelo A, et al. Saurian papulosis: a new clinicopathological entity. J Am Acad Dermatol. 2013;68:e17-22 pubmed publisher
immunohistochemistry; human	Qi H, Zheng X, Yuan X, Pflugfelder S, Li D. Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium. J Cell Physiol. 2010;225:180-5 pubmed publisher
immunocytochemistry; human immunohistochemistry; human western blot; human	Araya J, Cambier S, Markovics J, Wolters P, Jablons D, Hill A, et al. Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients. J Clin Invest. 2007;117:3551-62 pubmed
immunohistochemistry; human; 1:100	Pontiggia L, Klar A, Böttcher-Haberzeth S, Biedermann T, Meuli M, Reichmann E. Optimizing in vitro culture conditions leads to a significantly shorter production time of human dermo-epidermal skin substitutes. Pediatr Surg Int. 2013;29:249-56 pubmed publisher
immunohistochemistry; human; 1:50	Dhong E, Hwang N, Kim D, Rajashekhar G, Johnstone B, March K. Morphologic changes in photodamaged organotypic human skin culture after treatment of autologous adipose-derived stromal cells. J Craniofac Surg. 2012;23:805-11 pubmed publisher
western blot; human; fig 3	Wilson V. Growth and differentiation of HaCaT keratinocytes. Methods Mol Biol. 2014;1195:33-41 pubmed publisher
western blot; human	Warters R, Packard A, Kramer G, Gaffney D, Moos P. Differential gene expression in primary human skin keratinocytes and fibroblasts in response to ionizing radiation. Radiat Res. 2009;172:82-95 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Kirschner N, Haftek M, Niessen C, Behne M, Furuse M, Moll I, et al. CD44 regulates tight-junction assembly and barrier function. J Invest Dermatol. 2011;131:932-43 pubmed publisher
immunohistochemistry; human	Hachiya A, Sriwiriyanont P, Patel A, Saito N, Ohuchi A, Kitahara T, et al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther. 2007;14:648-56 pubmed
immunocytochemistry; human; 1:40	Chen Z, Mok H, Pflugfelder S, Li D, Barry M. Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors. Exp Eye Res. 2006;83:798-806 pubmed
immunocytochemistry; human; 1:200	Hayashi N, Kido J, Kido R, Wada C, Kataoka M, Shinohara Y, et al. Regulation of calprotectin expression by interleukin-1alpha and transforming growth factor-beta in human gingival keratinocytes. J Periodontal Res. 2007;42:1-7 pubmed
immunohistochemistry; human; 1:100	Tharakan S, Pontiggia L, Biedermann T, Böttcher-Haberzeth S, Schiestl C, Reichmann E, et al. Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells. Pediatr Surg Int. 2010;26:71-7 pubmed publisher
immunocytochemistry; sheep	Islam M, Zhou H. Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus. Eur J Dermatol. 2007;17:302-8 pubmed
immunohistochemistry; human; 200 mg/ml	Mitchell D, Israr M, Alam S, Kishel J, Dinello D, Meyers C. Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium. HIV Med. 2012;13:276-90 pubmed publisher
western blot; human; 1:100	Beckstead B, Tung J, Liang K, Tavakkol Z, Usui M, Olerud J, et al. Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model. J Biomed Mater Res A. 2009;91:436-46 pubmed publisher
immunocytochemistry; human; 1:100	Bulysheva A, Bowlin G, Klingelhutz A, Yeudall W. Low-temperature electrospun silk scaffold for in vitro mucosal modeling. J Biomed Mater Res A. 2012;100:757-67 pubmed publisher
western blot; rat; 1:100	Beckstead B, Santosa D, Giachelli C. Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation. J Biomed Mater Res A. 2006;79:94-103 pubmed
western blot; human	Cheung P, Yip Y, Tsao S, Ching Y, Cheung A. Id-1 induces cell invasiveness in immortalized epithelial cells by regulating cadherin switching and Rho GTPases. J Cell Biochem. 2011;112:157-68 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Senoo M, Pinto F, Crum C, McKeon F. p63 Is essential for the proliferative potential of stem cells in stratified epithelia. Cell. 2007;129:523-36 pubmed
immunocytochemistry; human; 1:40	Chen Z, Evans W, Pflugfelder S, Li D. Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells. Stem Cells. 2006;24:1265-73 pubmed
	Hinterhuber G, Marquardt Y, Diem E, Rappersberger K, Wolff K, Foedinger D. Organotypic keratinocyte coculture using normal human serum: an immunomorphological study at light and electron microscopic levels. Exp Dermatol. 2002;11:413-20 pubmed
	Chen Z, de Paiva C, Luo L, Kretzer F, Pflugfelder S, Li D. Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells. 2004;22:355-66 pubmed
	Asai Y, Ohyama Y, Gen K, Ogawa T. Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2. Infect Immun. 2001;69:7387-95 pubmed
	D'Silva N, Mitra R, Zhang Z, Kurnit D, Babcock C, Polverini P, et al. Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes. J Cell Physiol. 2003;196:532-40 pubmed
	Mizoguchi M, Suga Y, Sanmano B, Ikeda S, Ogawa H. Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis. J Dermatol Sci. 2004;35:199-206 pubmed
	Kee S, Steinert P. Microtubule disruption in keratinocytes induces cell-cell adhesion through activation of endogenous E-cadherin. Mol Biol Cell. 2001;12:1983-93 pubmed
	Hale L, Markert M. Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus. J Immunol. 2004;172:617-24 pubmed
	Uzgare A, Isaacs J. Enhanced redundancy in Akt and mitogen-activated protein kinase-induced survival of malignant versus normal prostate epithelial cells. Cancer Res. 2004;64:6190-9 pubmed
	Hale L, Buckley R, Puck J, Patel D. Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency. Clin Immunol. 2004;110:63-70 pubmed

product information

Product Type :

Antibody

Product Name :

Involucrin Monoclonal Antibody (SY5), Biotin

Catalog # :

MA5-11800

Quantity :

500µL

Price :

US 375.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Canine, Human, Non-human primate, Porcine, Rat

Applications :

ELISA: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 1:100-200, Immunoprecipitation: 2 µg/ml, Western Blot: 1-2 µg/ml

Species :

Canine, Human, Non-human primate, Porcine, Rat

Clone :

SY5

Isotype :

IgG1

Storage :

4° C

Description :

Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Immunogen :

Human keratinocytes' involucrin

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 1:100-200, Immunoprecipitation: 2 µg/ml, Western Blot: 1-2 µg/ml

PubMed References :

11451997, 11705912, 12366694, 12891710, 14688374, 14962797, 15153612, 15342404, 15381241, 15619432, 15675970, 15781286, 16424398, 16758464, 16793039, 17214633, 17268532, 17482546, 17540636, 17965775, 18771458, 18985776, 19580510, 19686061, 19856181, 20376062, 20458737, 20725041, 21053361, 21191420, 21982635, 22238242, 22276657, 22565925, 23377785, 24155234, 24847782