CFTR Monoclonal Antibody (M3A7) | Invitrogen MA5-11768 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CFTR Monoclonal Antibody (M3A7)

catalog :

MA5-11768

quantity :

500 µL

price :

US 430

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M3A7

reactivity :

American opossums, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; American opossums; 1:100	Fan S, Harfoot N, Bartolo R, Butt A. CFTR is restricted to a small population of high expresser cells that provide a forskolin-sensitive transepithelial Cl- conductance in the proximal colon of the possum, Trichosurus vulpecula. J Exp Biol. 2012;215:1218-30 pubmed publisher
immunocytochemistry; human	Cardinale V, Wang Y, Carpino G, Cui C, Gatto M, Rossi M, et al. Multipotent stem/progenitor cells in human biliary tree give rise to hepatocytes, cholangiocytes, and pancreatic islets. Hepatology. 2011;54:2159-72 pubmed publisher
immunohistochemistry; American opossums; 2 ug/ml western blot; American opossums; 1:100	Gill M, Bartolo R, Demmers K, Harfoot N, Fan S, Butt G. The distribution and expression of CFTR restricts electrogenic anion secretion to the ileum of the brushtail possum, Trichosurus vulpecula. J Exp Biol. 2011;214:1943-54 pubmed publisher
immunohistochemistry; rat; 1:50	Ham M, Mizumori M, Watanabe C, Wang J, Inoue T, Nakano T, et al. Endogenous luminal surface adenosine signaling regulates duodenal bicarbonate secretion in rats. J Pharmacol Exp Ther. 2010;335:607-13 pubmed publisher
immunohistochemistry; rat; 1:100 immunohistochemistry; human; 1:100	Su M, Guo Y, Zhao Y, Korteweg C, Gu J. Expression of cystic fibrosis transmembrane conductance regulator in paracervical ganglia. Biochem Cell Biol. 2010;88:747-55 pubmed publisher
western blot; American opossums; 1:2000	Demmers K, Carter D, Fan S, Mao P, Maqbool N, McLeod B, et al. Molecular and functional characterization of the cystic fibrosis transmembrane conductance regulator from the Australian common brushtail possum, Trichosurus vulpecula. J Comp Physiol B. 2010;180:545-61 pubmed publisher
immunohistochemistry; human; 1:100	Guo Y, Su M, McNutt M, Gu J. Expression and distribution of cystic fibrosis transmembrane conductance regulator in neurons of the human brain. J Histochem Cytochem. 2009;57:1113-20 pubmed publisher
immunohistochemistry; human; 1:100	Guo Y, Su M, Su M, McNutt M, Gu J. Expression and distribution of cystic fibrosis transmembrane conductance regulator in neurons of the spinal cord. J Neurosci Res. 2009;87:3611-9 pubmed publisher
immunohistochemistry; mouse; 1:100	Glaser S, Gaudio E, Rao A, Pierce L, Onori P, Franchitto A, et al. Morphological and functional heterogeneity of the mouse intrahepatic biliary epithelium. Lab Invest. 2009;89:456-69 pubmed publisher
immunohistochemistry; rat; 1:100	Pan P, Guo Y, Gu J. Expression of cystic fibrosis transmembrane conductance regulator in ganglion cells of the hearts. Neurosci Lett. 2008;441:35-8 pubmed publisher
immunocytochemistry; mouse; 1:50	Francis H, Glaser S, Demorrow S, Gaudio E, Ueno Y, Venter J, et al. Small mouse cholangiocytes proliferate in response to H1 histamine receptor stimulation by activation of the IP3/CaMK I/CREB pathway. Am J Physiol Cell Physiol. 2008;295:C499-513 pubmed publisher
	Mauduit O, Aure M, Delcroix V, Basova L, Srivastava A, Umazume T, et al. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep. 2022;39:110663 pubmed publisher

image

image 1 :

Formalin-fixed, paraffin-embedded human pancreas stained with CFTR antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of epithelial cells in the intercalated ducts.

product information

Product Type :

Antibody

Product Name :

CFTR Monoclonal Antibody (M3A7)

Catalog # :

MA5-11768

Quantity :

500 µL

Pricing :

US 430

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1:12.5-25, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human, Mouse, Rat

Clone :

M3A7

Isotype :

IgG1

Storage :

4° C

Description :

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.

Immunogen :

Recombinant protein encoding NBF2 domain of human CFTR

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:12.5-25, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

ABC35; Abcc7; ATP Binding Cassette Superfamily C Member 7 (ABCC7); ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; ATP-binding cassette, subfamily c, member 7; AW495489; cAMP-dependent chloride channel; CF; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); cystic fibrosis transmembrane conductance regulator homolog; cystic fibrosis transmembrane conductance regulator homolog; ATP-binding cassette, subfamily c, member 7; dJ760C5.1; MRP7; RGD1561193; tcag7.78; TNR CFTR; TNR-CFTR

more info or order :

Invitrogen product webpage