SREBP1 Monoclonal Antibody (2A4) | Invitrogen MA5-11685 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SREBP1 Monoclonal Antibody (2A4)

catalog :

MA5-11685

quantity :

500 µL

price :

US 419

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A4

reactivity :

goose, Chinese hamsters, human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 6d	Yang Z, Tsuchiya H, Zhang Y, Lee S, Liu C, Huang Y, et al. REV-ERB? Activates C/EBP Homologous Protein to Control Small Heterodimer Partner-Mediated Oscillation of Alcoholic Fatty Liver. Am J Pathol. 2016;186:2909-2920 pubmed publisher
western blot; mouse; loading ...; fig 2b	Hsieh J, Koseki M, Molusky M, Yakushiji E, Ichi I, Westerterp M, et al. TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis. Nature. 2016;535:303-7 pubmed publisher
western blot; mouse	Bojic L, Telford D, Fullerton M, Ford R, Sutherland B, Edwards J, et al. PPAR? activation attenuates hepatic steatosis in Ldlr-/- mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity. J Lipid Res. 2014;55:1254-66 pubmed publisher
western blot; human; 1:1000	Poirier S, Samami S, Mamarbachi M, Demers A, Chang T, Vance D, et al. The epigenetic drug 5-azacytidine interferes with cholesterol and lipid metabolism. J Biol Chem. 2014;289:18736-51 pubmed publisher
western blot; goose; 1:1000	Han C, Wang J, Pan Z, Tang H, Xiang S, Wang J, et al. Effect of cholesterol on lipogenesis and VLDL-TG assembly and secretion in goose primary hepatocytes. Mol Cell Biochem. 2013;374:163-72 pubmed publisher
western blot; human	Beyea M, Reaume S, Sawyez C, Edwards J, O Neil C, Hegele R, et al. The oxysterol 24(s),25-epoxycholesterol attenuates human smooth muscle-derived foam cell formation via reduced low-density lipoprotein uptake and enhanced cholesterol efflux. J Am Heart Assoc. 2012;1:e000810 pubmed publisher
immunohistochemistry; mouse	Tu K, Zheng X, Yin G, Zan X, Yao Y, Liu Q. Evaluation of Fbxw7 expression and its correlation with expression of SREBP-1 in a mouse model of NAFLD. Mol Med Rep. 2012;6:525-30 pubmed publisher
western blot; mouse; 1.5 ug/ml	Lundåsen T, Andersson E, Snaith M, Lindmark H, Lundberg J, Ostlund Lindqvist A, et al. Inhibition of intestinal bile acid transporter Slc10a2 improves triglyceride metabolism and normalizes elevated plasma glucose levels in mice. PLoS ONE. 2012;7:e37787 pubmed publisher
western blot; mouse	Benhamed F, Denechaud P, Lemoine M, Robichon C, Moldes M, Bertrand Michel J, et al. The lipogenic transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans. J Clin Invest. 2012;122:2176-94 pubmed publisher
western blot; mouse	Dentin R, Tomás Cobos L, Foufelle F, Leopold J, Girard J, Postic C, et al. Glucose 6-phosphate, rather than xylulose 5-phosphate, is required for the activation of ChREBP in response to glucose in the liver. J Hepatol. 2012;56:199-209 pubmed publisher
western blot; rat	Johnson K, McDowell E, Viereck M, Xia J. Species-specific dibutyl phthalate fetal testis endocrine disruption correlates with inhibition of SREBP2-dependent gene expression pathways. Toxicol Sci. 2011;120:460-74 pubmed publisher
western blot; mouse	Dubuquoy C, Robichon C, Lasnier F, Langlois C, Dugail I, Foufelle F, et al. Distinct regulation of adiponutrin/PNPLA3 gene expression by the transcription factors ChREBP and SREBP1c in mouse and human hepatocytes. J Hepatol. 2011;55:145-53 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Onoyama I, Suzuki A, Matsumoto A, Tomita K, Katagiri H, Oike Y, et al. Fbxw7 regulates lipid metabolism and cell fate decisions in the mouse liver. J Clin Invest. 2011;121:342-54 pubmed publisher
western blot; human	Lee J, Brauweiler A, Rudolph M, Hooper J, Drabkin H, Gemmill R. The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways. Mol Cancer Res. 2010;8:93-106 pubmed publisher
western blot; human	Lerat H, Kammoun H, Hainault I, Merour E, Higgs M, Callens C, et al. Hepatitis C virus proteins induce lipogenesis and defective triglyceride secretion in transgenic mice. J Biol Chem. 2009;284:33466-74 pubmed publisher
western blot; mouse	Kammoun H, Chabanon H, Hainault I, Luquet S, Magnan C, Koike T, et al. GRP78 expression inhibits insulin and ER stress-induced SREBP-1c activation and reduces hepatic steatosis in mice. J Clin Invest. 2009;119:1201-15 pubmed publisher
western blot; mouse	Scapa E, Pocai A, Wu M, Gutierrez Juarez R, Glenz L, Kanno K, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD2. FASEB J. 2008;22:2579-90 pubmed publisher
western blot; mouse	Dentin R, Benhamed F, Hainault I, Fauveau V, Foufelle F, Dyck J, et al. Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice. Diabetes. 2006;55:2159-70 pubmed
western blot; rat	Gregori C, Guillet Deniau I, Girard J, Decaux J, Pichard A. Insulin regulation of glucokinase gene expression: evidence against a role for sterol regulatory element binding protein 1 in primary hepatocytes. FEBS Lett. 2006;580:410-4 pubmed
western blot; mouse	Dentin R, Benhamed F, Pégorier J, Foufelle F, Viollet B, Vaulont S, et al. Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation. J Clin Invest. 2005;115:2843-54 pubmed
western blot; human	Le Jossic Corcos C, Gonthier C, Zaghini I, Logette E, Shechter I, Bournot P. Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids. Biochem J. 2005;385:787-94 pubmed
	Budge J, Roobol J, Singh G, Mozzanino T, Knight T, Povey J, et al. A proline metabolism selection system and its application to the engineering of lipid biosynthesis in Chinese hamster ovary cells. Metab Eng Commun. 2021;13:e00179 pubmed publisher
	Lewandowski C, Khan M, BenAissa M, Dubrovskyi O, Ackerman Berrier M, Ladu M, et al. Metabolomic analysis of a selective ABCA1 inducer in obesogenic challenge provides a rationale for therapeutic development. EBioMedicine. 2021;66:103287 pubmed publisher
	Tiscione S, Vivas O, Ginsburg K, Bers D, Ory D, Santana L, et al. Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. J Cell Biol. 2019;218:4141-4156 pubmed publisher
	Cook E, Nelson J, Sorrentino V, Koenis D, Moeton M, Scheij S, et al. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE. 2017;12:e0172721 pubmed publisher
	Ettinger S, Sobel R, Whitmore T, Akbari M, Bradley D, Gleave M, et al. Dysregulation of sterol response element-binding proteins and downstream effectors in prostate cancer during progression to androgen independence. Cancer Res. 2004;64:2212-21 pubmed
	Dentin R, Pégorier J, Benhamed F, Foufelle F, Ferre P, Fauveau V, et al. Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression. J Biol Chem. 2004;279:20314-26 pubmed

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product information

Product Type :

Antibody

Product Name :

SREBP1 Monoclonal Antibody (2A4)

Catalog # :

MA5-11685

Quantity :

500 µL

Pricing :

US 419

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Hamster, Human

Applications :

Western Blot: 1-2 µg/mL

Species :

Hamster, Human

Clone :

2A4

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Immunogen :

Recombinant protein encoding N-terminal amino acids 301-407 (the bHLH/leucine zipper domain) of human SREBP-1

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1-2 µg/mL

Aliases :

ADD1; ADD-1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; I79_010155; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBF1; SREBP1; SREBP-1; SREBP1a; SREBP-1a; SREBP1c; SREBP-1c; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1; sterol regulatory element binding-protein 1; sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1

more info or order :

Invitrogen product webpage