Cytochrome C Monoclonal Antibody (7H8.2C12) | Invitrogen MA5-11674 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cytochrome C Monoclonal Antibody (7H8.2C12)

catalog :

MA5-11674

quantity :

500 µL

price :

US 436.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H8.2C12

reactivity :

human, mouse, rat, fruit fly , Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 40

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	Arora R, Sawney S, Saini V, Steffi C, Tiwari M, Saluja D. Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1. Mol Cancer. 2016;15:64 pubmed
immunohistochemistry - paraffin section; rat; fig 4	Galal A, El Bakly W, Al Haleem E, El Demerdash E. Selective A3 adenosine receptor agonist protects against doxorubicin-induced cardiotoxicity. Cancer Chemother Pharmacol. 2016;77:309-22 pubmed publisher
immunohistochemistry - paraffin section; rat; fig 5	Mahran Y, El Demerdash E, Nada A, El Naga R, Ali A, Abdel Naim A. Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis. PLoS ONE. 2015;10:e0140055 pubmed publisher
immunohistochemistry; rat	Said R, Badr A, Nada A, El Demerdash E. Sodium selenite treatment restores long-lasting ovarian damage induced by irradiation in rats: impact on oxidative stress and apoptosis. Reprod Toxicol. 2014;43:85-93 pubmed publisher
immunohistochemistry; rat; fig 6	Said R, Nada A, El Demerdash E. Sodium selenite improves folliculogenesis in radiation-induced ovarian failure: a mechanistic approach. PLoS ONE. 2012;7:e50928 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Jung Y, Joo K, Seong D, Choi Y, Kong D, Kim Y, et al. Identification of prognostic biomarkers for glioblastomas using protein expression profiling. Int J Oncol. 2012;40:1122-32 pubmed publisher
western blot; rat; 1:1000	Chen Y, Aon M, Hsu Y, Soane L, Teng X, McCaffery J, et al. Bcl-xL regulates mitochondrial energetics by stabilizing the inner membrane potential. J Cell Biol. 2011;195:263-76 pubmed publisher
western blot; human	Chang S, Shen S, Lee W, Yang L, Chen Y. Imatinib mesylate induction of ROS-dependent apoptosis in melanoma B16F0 cells. J Dermatol Sci. 2011;62:183-91 pubmed publisher
western blot; Xenopus laevis	Tsuchiya Y, Yamashita S. Anti-apoptotic activity and proteasome-mediated degradation of Xenopus Mcl-1 protein in egg extracts. J Biol Chem. 2011;286:15806-14 pubmed publisher
western blot; human	Hashiramoto A, Murata M, Kawazoe T, Yoshida K, Akiyama C, Shiozawa K, et al. Heat shock protein 90 maintains the tumour-like character of rheumatoid synovial cells by stabilizing integrin-linked kinase, extracellular signal-regulated kinase and protein kinase B. Rheumatology (Oxford). 2011;50:852-61 pubmed publisher
immunocytochemistry; human; 1:100	Redondo S, Ruiz E, Gordillo Moscoso A, Navarro Dorado J, Ramajo M, Rodriguez E, et al. Overproduction of cyclo-oxygenase-2 (COX-2) is involved in the resistance to apoptosis in vascular smooth muscle cells from diabetic patients: a link between inflammation and apoptosis. Diabetologia. 2011;54:190-9 pubmed publisher
western blot; rat; 1:1000; fig 15	Guerrero Beltrán C, Calderon Oliver M, Martínez Abundis E, Tapia E, Zarco Márquez G, Zazueta C, et al. Protective effect of sulforaphane against cisplatin-induced mitochondrial alterations and impairment in the activity of NAD(P)H: quinone oxidoreductase 1 and ? glutamyl cysteine ligase: studies in mitochondria isolated from rat kidney and in LLC-PK1 . Toxicol Lett. 2010;199:80-92 pubmed publisher
western blot; human	Wu P, Tian Y, Chen G, Wang B, Gui L, Xi L, et al. Ubiquitin B: an essential mediator of trichostatin A-induced tumor-selective killing in human cancer cells. Cell Death Differ. 2010;17:109-18 pubmed publisher
western blot; rat; 1:2000; tbl 1	Zucchini Pascal N, De Sousa G, Rahmani R. Lindane and cell death: at the crossroads between apoptosis, necrosis and autophagy. Toxicology. 2009;256:32-41 pubmed publisher
western blot; rat; 1:200	Fu Y, Zhang Z, Zhang G, Liu Y, Cao Y, Yu J, et al. Adenovirus-mediated gene transfer of tissue factor pathway inhibitor induces apoptosis in vascular smooth muscle cells. Apoptosis. 2008;13:634-40 pubmed publisher
immunohistochemistry; fruit fly ; 1:200 western blot; fruit fly ; 1:500	Abdelwahid E, Yokokura T, Krieser R, Balasundaram S, Fowle W, White K. Mitochondrial disruption in Drosophila apoptosis. Dev Cell. 2007;12:793-806 pubmed
immunocytochemistry; rat; 1:300	López Sánchez N, Rodriguez J, Frade J. Mitochondrial c-Jun NH2-terminal kinase prevents the accumulation of reactive oxygen species and reduces necrotic damage in neural tumor cells that lack trophic support. Mol Cancer Res. 2007;5:47-60 pubmed
western blot; mouse	Matsumoto T, Hayasaki T, Nishimura Y, Nakamura M, Takeda T, Tabuchi Y, et al. Butyrate induces necrotic cell death in murine colonic epithelial cell MCE301. Biol Pharm Bull. 2006;29:2041-5 pubmed
western blot; human; 1:500	Richard E, Monteoliva L, Juarez S, Perez B, Desviat L, Ugarte M, et al. Quantitative analysis of mitochondrial protein expression in methylmalonic acidemia by two-dimensional difference gel electrophoresis. J Proteome Res. 2006;5:1602-10 pubmed
immunocytochemistry; mouse western blot; mouse	Yasuda O, Fukuo K, Sun X, Nishitani M, Yotsui T, Higuchi M, et al. Apop-1, a novel protein inducing cyclophilin D-dependent but Bax/Bak-related channel-independent apoptosis. J Biol Chem. 2006;281:23899-907 pubmed
western blot; rat; 1:400; fig 6	Tanaka S, Takehashi M, Iida S, Kitajima T, Kamanaka Y, Stedeford T, et al. Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation. J Neurochem. 2005;95:179-90 pubmed
western blot; human	Roucou X, Giannopoulos P, Zhang Y, Jodoin J, Goodyer C, Leblanc A. Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells. Cell Death Differ. 2005;12:783-95 pubmed
western blot; mouse; 1:1000	Masubuchi Y, Suda C, Horie T. Involvement of mitochondrial permeability transition in acetaminophen-induced liver injury in mice. J Hepatol. 2005;42:110-6 pubmed
western blot; rat	Schamberger C, Gerner C, Cerni C. Caspase-9 plays a marginal role in serum starvation-induced apoptosis. Exp Cell Res. 2005;302:115-28 pubmed
western blot; mouse; fig 4	Abarca Rojano E, Rosas Medina P, Zamudio Cortéz P, Mondragón Flores R, Sánchez García F. Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages. Scand J Immunol. 2003;58:419-27 pubmed
western blot; rat; 1:200; fig 3	Olichon A, Emorine L, Descoins E, Pelloquin L, Brichese L, Gas N, et al. The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space. FEBS Lett. 2002;523:171-6 pubmed
western blot; human; fig 2	Tang D, Lahti J, Grenet J, Kidd V. Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism. J Biol Chem. 1999;274:7245-52 pubmed
	Rana R, Huirem R, Kant R, Chauhan K, Sharma S, Yashavarddhan M, et al. Cytochrome C as a potential clinical marker for diagnosis and treatment of glioma. Front Oncol. 2022;12:960787 pubmed publisher
	Ne x17e i x107 L, Amid x17e i x107 L, x160 krbi x107 R, Gajanin R, Mandi x107 D, Dumanovi x107 J, et al. Amelioration of Endotoxin-Induced Acute Lung Injury and Alveolar Epithelial Cells Apoptosis by Simvastatin Is Associated with Up-Regulation of Survivin/NF-kB/p65 Pathway. Int J Mol Sci. 2022;23: pubmed publisher
	Donati G, Ravà M, Filipuzzi M, Nicoli P, Cassina L, Verrecchia A, et al. Targeting mitochondrial respiration and the BCL2 family in high-grade MYC-associated B-cell lymphoma. Mol Oncol. 2021;: pubmed publisher
	Abedin M, Powers K, Aiardo R, Barua D, Barua S. Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells. Sci Rep. 2021;11:7347 pubmed publisher
	Nezić L, Skrbic R, Amidzić L, Gajanin R, Milovanović Z, Nepovimova E, et al. Protective Effects of Simvastatin on Endotoxin-Induced Acute Kidney Injury through Activation of Tubular Epithelial Cells' Survival and Hindering Cytochrome C-Mediated Apoptosis. Int J Mol Sci. 2020;21: pubmed publisher
	Du J, Gao R, Wang Y, Nguyen T, Yang F, Shi Y, et al. MicroRNA-26a/b have protective roles in oral lichen planus. Cell Death Dis. 2020;11:15 pubmed publisher
	Bayraktar E, Baudrier L, Özerdem C, Lewis C, Chan S, Kunchok T, et al. MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo. Proc Natl Acad Sci U S A. 2019;116:303-312 pubmed publisher
	Suwa M, Nakano H, Radak Z, Kumagai S. Effects of Nitric Oxide Synthase Inhibition on Fiber-Type Composition, Mitochondrial Biogenesis, and SIRT1 Expression in Rat Skeletal Muscle. J Sports Sci Med. 2015;14:548-55 pubmed
	Grant J, Saldanha J, Gould A. A Drosophila model for primary coenzyme Q deficiency and dietary rescue in the developing nervous system. Dis Model Mech. 2010;3:799-806 pubmed publisher
	Gorsic M, Majdic G, Komel R. Identification of differentially expressed genes in 4-day axolotl limb blastema by suppression subtractive hybridization. J Physiol Biochem. 2008;64:37-50 pubmed
	Tsai H, Tsai H, Hsieh M. Full-length expanded ataxin-3 enhances mitochondrial-mediated cell death and decreases Bcl-2 expression in human neuroblastoma cells. Biochem Biophys Res Commun. 2004;324:1274-82 pubmed
	Andoniou C, Andrews D, Manzur M, Ricciardi Castagnoli P, Degli Esposti M. A novel checkpoint in the Bcl-2-regulated apoptotic pathway revealed by murine cytomegalovirus infection of dendritic cells. J Cell Biol. 2004;166:827-37 pubmed
	Zhang J, Zhou F, Song Y, Ying W, Zhang Y. Long dwell-time exposure of human chorionic villi to transvaginal ultrasound in the first trimester of pregnancy induces activation of caspase-3 and cytochrome C release. Biol Reprod. 2002;67:580-3 pubmed

product information

Product Type :

Antibody

Product Name :

Cytochrome C Monoclonal Antibody (7H8.2C12)

Catalog # :

MA5-11674

Quantity :

500 µL

Price :

US 436.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:100, Western Blot: Assay-dependent

Species :

Human, Mouse

Clone :

7H8.2C12

Isotype :

IgG2b, kappa

Storage :

4° C

Description :

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Immunogen :

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon cytochrome c

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:100, Western Blot: Assay-dependent

Aliases :

CG17903; CG17903-PA; CG17903-PB; CRF; CYC; CYC2; Cycs; CYCSA; Cyct-c-p; Cyt c; cyt c-p; cyt.c; CytC; Cyt-c; CYTC2; Cyt-c2; CytC-2; cyt-c-p; cytC-p; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome c proximal; Cytochrome C, expressed in somatic tissues; cytochrome c, somatic; Cytochrome c-2; cytochrome c-like; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; DC4; dCyt.C-p; DMc01; Dmel CG17903; Dmel_CG17903; hc1; HCS; l(2)k13905; lethal (2) k13905; LOC100341175; LOC100357840; MGC128356 protein; pDMc01; RH17228p; THC4

more info or order :

Invitrogen product webpage