Granzyme B Monoclonal Antibody (GZB01) | Invitrogen MA5-11587 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Granzyme B Monoclonal Antibody (GZB01)

catalog :

MA5-11587

quantity :

500 µL

price :

US 471

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GZB01

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:50; fig 3	Golombeck K, Bönte K, Mönig C, van Loo K, Hartwig M, Schwindt W, et al. Evidence of a pathogenic role for CD8(+) T cells in anti-GABAB receptor limbic encephalitis. Neurol Neuroimmunol Neuroinflamm. 2016;3:e232 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000	HÃ¶ftberger R, Leisser M, Bauer J, Lassmann H. Autoimmune encephalitis in humans: how closely does it reflect multiple sclerosis ?. Acta Neuropathol Commun. 2015;3:80 pubmed publisher
immunohistochemistry - paraffin section; human	Jeon Y, Kim J, Sung J, Han J, Ko Y. Epstein-Barr virus-positive nodal T/NK-cell lymphoma: an analysis of 15 cases with distinct clinicopathological features. Hum Pathol. 2015;46:981-90 pubmed publisher
immunohistochemistry; human; 1:100	Yang Y, Li Y, Liu Y, Yang M, Liu K. CD30+ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report. Oncol Lett. 2014;7:1419-1421 pubmed
immunohistochemistry; human	Abdou A, Shoeib M, Bakry O, El Bality H. Immunohistochemical expression of granzyme B and perforin in discoid lupus erythematosus. Ultrastruct Pathol. 2013;37:408-16 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000	Fischer M, Wimmer I, Hoftberger R, Gerlach S, Haider L, Zrzavy T, et al. Disease-specific molecular events in cortical multiple sclerosis lesions. Brain. 2013;136:1799-815 pubmed publisher
immunohistochemistry; human; 1:100	Wang T, Xu C, Liu S, Kan B, Ran Y, Liu W, et al. Clinicopathology, immunophenotype, T cell receptor gene rearrangement, Epstein-Barr virus status and p53 gene mutation of cutaneous extranodal NK/T-cell lymphoma, nasal-type. Chin Med J (Engl). 2013;126:1281-7 pubmed
immunohistochemistry; human; 1:25	Song H, Huang W, Chen Y, Chang K. Tumorous proliferations of plasmacytoid dendritic cells and Langerhans cells associated with acute myeloid leukaemia. Histopathology. 2012;61:974-83 pubmed publisher
immunohistochemistry; human; 1:1000	Berghoff A, Lassmann H, Preusser M, Hoftberger R. Characterization of the inflammatory response to solid cancer metastases in the human brain. Clin Exp Metastasis. 2013;30:69-81 pubmed publisher
immunohistochemistry; human	Yu J, Zhang Y, Yang Q, Wang X, Tang Y, Zhao S, et al. Invasion-associated genes identified by gene expression profiling in extranodal natural killer/T-cell lymphoma, nasal type. Leuk Lymphoma. 2013;54:90-8 pubmed publisher
immunohistochemistry; human; fig 3	Lee D, Yang J, Lee S, Won C, Chang S, Lee M, et al. Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients. Ann Dermatol. 2011;23:329-37 pubmed publisher
immunohistochemistry; human; 1:50; fig 5I	Ruffell B, Au A, Rugo H, Esserman L, Hwang E, Coussens L. Leukocyte composition of human breast cancer. Proc Natl Acad Sci U S A. 2012;109:2796-801 pubmed publisher
immunohistochemistry; human	Lu C, Tang Y, Yang Q, Wang M, Zhao S, Bi C, et al. Hepatosplenic T-cell lymphoma: clinicopathologic, immunophenotypic, and molecular characterization of 17 Chinese cases. Hum Pathol. 2011;42:1965-78 pubmed publisher
immunohistochemistry; human; 1:20	Prabhu S, Gupta P, Durgapal H, Rath S, Gupta S, Acharya S, et al. Study of cellular immune response against Hepatitis E virus (HEV). J Viral Hepat. 2011;18:587-94 pubmed publisher
immunohistochemistry; human	Pozdzik A, Berton A, Schmeiser H, Missoum W, Decaestecker C, Salmon I, et al. Aristolochic acid nephropathy revisited: a place for innate and adaptive immunity?. Histopathology. 2010;56:449-63 pubmed publisher
immunohistochemistry; human	Estes J, Gordon S, Zeng M, Chahroudi A, Dunham R, Staprans S, et al. Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques. J Immunol. 2008;180:6798-807 pubmed
immunohistochemistry; human	Derfuss T, Segerer S, Herberger S, Sinicina I, Hüfner K, Ebelt K, et al. Presence of HSV-1 immediate early genes and clonally expanded T-cells with a memory effector phenotype in human trigeminal ganglia. Brain Pathol. 2007;17:389-98 pubmed
	Amoh Y, Hamada Y, Katsuoka K, Hoffman R. In vivo imaging of nuclear-cytoplasmic deformation and partition during cancer cell death due to immune rejection. J Cell Biochem. 2012;113:465-72 pubmed publisher
	Wanschitz J, Maier H, Lassmann H, Budka H, Berger T. Distinct time pattern of complement activation and cytotoxic T cell response in Guillain-Barré syndrome. Brain. 2003;126:2034-42 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human lymphoma stained with Granzyme B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

product information

Product Type :

Antibody

Product Name :

Granzyme B Monoclonal Antibody (GZB01)

Catalog # :

MA5-11587

Quantity :

500 µL

Pricing :

US 471

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 1:25-1:50, Western Blot: 1:500

Species :

Human

Clone :

GZB01

Isotype :

IgG2a

Storage :

4° C

Description :

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

Immunogen :

Recombinant protein encoding the N-terminus of the mature granzyme B

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:25-1:50, Western Blot: 1:500

Aliases :

AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E

more info or order :

Invitrogen product webpage