CDK1 Monoclonal Antibody (A17.1.1), Biotin | Invitrogen MA5-11469 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CDK1 Monoclonal Antibody (A17.1.1), Biotin

catalog :

MA5-11469

quantity :

500µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

A17.1.1

reactivity :

Domestic guinea pig, human, mouse, rat, chicken, common platanna

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Cinti C, Vindigni C, Zamparelli A, La Sala D, Epistolato M, Marrelli D, et al. Activated Akt as an indicator of prognosis in gastric cancer. Virchows Arch. 2008;453:449-55 pubmed publisher
western blot; human	Choi J, Murillo G, Su B, Pezzuto J, Kinghorn A, Mehta R. Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells. FEBS J. 2006;273:5714-23 pubmed
immunohistochemistry; human; 1:500	Nakamura N, Yamamoto H, Yao T, Oda Y, Nishiyama K, Imamura M, et al. Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade. Hum Pathol. 2005;36:828-37 pubmed
western blot; human; 1:200	Raynaud F, Whittaker S, Fischer P, McClue S, Walton M, Barrie S, et al. In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202. Clin Cancer Res. 2005;11:4875-87 pubmed
	Whittaker S, Walton M, Garrett M, Workman P. The Cyclin-dependent kinase inhibitor CYC202 (R-roscovitine) inhibits retinoblastoma protein phosphorylation, causes loss of Cyclin D1, and activates the mitogen-activated protein kinase pathway. Cancer Res. 2004;64:262-72 pubmed
	Sheen J, Woo J, Dickson R. c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells. Br J Cancer. 2003;89:1479-85 pubmed
	Petermann A, Pippin J, Hiromura K, Monkawa T, Durvasula R, Couser W, et al. Mitotic cell cycle proteins increase in podocytes despite lack of proliferation. Kidney Int. 2003;63:113-22 pubmed
	Sandal T, Stapnes C, Kleivdal H, Hedin L, Døskeland S. A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells. J Biol Chem. 2002;277:20783-93 pubmed
	Leoncini L, Lazzi S, Scano D, Mura A, Onida A, Massarelli G, et al. Expression of the ALK protein by anaplastic large-cell lymphomas correlates with high proliferative activity. Int J Cancer. 2000;86:777-81 pubmed
	Leoncini L, Bellan C, Cossu A, Claudio P, Lazzi S, Cinti C, et al. Retinoblastoma-related p107 and pRb2/p130 proteins in malignant lymphomas: distinct mechanisms of cell growth control. Clin Cancer Res. 1999;5:4065-72 pubmed
	Leoncini L, Cossu A, Megha T, Bellan C, Lazzi S, Luzi P, et al. Expression of p34(cdc2) and cyclins A and B compared to other proliferative features of non-Hodgkin's lymphomas: a multivariate cluster analysis. Int J Cancer. 1999;83:203-9 pubmed

product information

Product Type :

Antibody

Product Name :

CDK1 Monoclonal Antibody (A17.1.1), Biotin

Catalog # :

MA5-11469

Quantity :

500µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Chicken, Guinea Pig, Human, Mouse, Rat, Rodent, Xenopus laevis

Applications :

Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Chicken, Guinea Pig, Human, Mouse, Rat, Rodent, Xenopus laevis

Clone :

A17.1.1

Isotype :

IgG2a

Storage :

4° C

Description :

CDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDK1 also play important regulatory roles in cell cycle control. Destruction of cyclin B during metaphase results in inactivation of CDK1, allowing mitotic exit and cell division. CDK1 also contributes to the control of DNA replication and can be inhibited by several transcriptional targets of p53, such as p21WAF. The ability of CDK1 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. Research studies have shown that expression of CDK1 in the brain has been linked to both neurogenesis and apoptosis. In vertebrates, multiple cyclins like CDK1 have been identified, including a total of eight such regulatory proteins in mammals. Alternatively spliced transcript variants encoding different isoforms of CDK1 have been found.

Immunogen :

C-Terminal 2/3 of Xenopus CDC2 expressed in E. coli

Format :

Liquid

Applications w/Dilutions :

Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

cell cycle controller CDC2, cell division control protein 2 homolog, cell division cycle 2, G1 to S and G2 to M, cell division protein kinase 1, p34 protein kinase