CD163 Monoclonal Antibody (10D6) | Invitrogen MA5-11458 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD163 Monoclonal Antibody (10D6)

catalog :

MA5-11458

quantity :

500 µL

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10D6

reactivity :

African green monkey, human, mouse, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 57

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 2e	Zhang M, Pan X, Fujiwara K, Jurcak N, Muth S, Zhou J, et al. Pancreatic cancer cells render tumor-associated macrophages metabolically reprogrammed by a GARP and DNA methylation-mediated mechanism. Signal Transduct Target Ther. 2021;6:366 pubmed publisher
immunohistochemistry - paraffin section; rhesus macaque; 1:400; loading ...; fig s6c	Chandrashekar A, Liu J, Martinot A, McMahan K, Mercado N, Peter L, et al. SARS-CoV-2 infection protects against rechallenge in rhesus macaques. Science. 2020;: pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig s4a	Cassetta L, Fragkogianni S, Sims A, Swierczak A, Forrester L, Zhang H, et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell. 2019;35:588-602.e10 pubmed publisher
immunocytochemistry; African green monkey; loading ...; fig 6c	Ferrando Martinez S, Moysi E, Pegu A, Andrews S, Nganou Makamdop K, Ambrozak D, et al. Accumulation of follicular CD8+ T cells in pathogenic SIV infection. J Clin Invest. 2018;128:2089-2103 pubmed publisher
immunohistochemistry; human; 1:50; loading ...; fig 1a	Giurisato E, Xu Q, Lonardi S, Telfer B, Russo I, Pearson A, et al. Myeloid ERK5 deficiency suppresses tumor growth by blocking protumor macrophage polarization via STAT3 inhibition. Proc Natl Acad Sci U S A. 2018;115:E2801-E2810 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:400; loading ...	Brocks T, Fedorchenko O, Schliermann N, Stein A, Moll U, Seegobin S, et al. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin. FASEB J. 2017;31:526-543 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Ang Z, Er J, Tan N, Lu J, Liou Y, Grosse J, et al. Human and mouse monocytes display distinct signalling and cytokine profiles upon stimulation with FFAR2/FFAR3 short-chain fatty acid receptor agonists. Sci Rep. 2016;6:34145 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; fig 6	Peda J, Salah S, Wallace D, Fields P, Grantham C, Fields T, et al. Autocrine IL-10 activation of the STAT3 pathway is required for pathological macrophage differentiation in polycystic kidney disease. Dis Model Mech. 2016;9:1051-61 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; loading ...; fig s1d	Salvi V, Vermi W, Gianello V, Lonardi S, Gagliostro V, Naldini A, et al. Dendritic cell-derived VEGF-A plays a role in inflammatory angiogenesis of human secondary lymphoid organs and is driven by the coordinated activation of multiple transcription factors. Oncotarget. 2016;7:39256-39269 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...	Ahmad N, Martin S, Storr S. Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis. Methods Mol Biol. 2016;1430:49-57 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 1c, d	Lin M, Yang C, Kuo S, Wu C, Chang Y, Yang P. The Prognostic Significance of pSTAT1 and CD163 Expressions in Surgically Resected Stage 1 Pulmonary Squamous Cell Carcinomas. Ann Surg Oncol. 2016;23:3071-81 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:300; tbl 1	Choi E, Hendley A, Bailey J, Leach S, Goldenring J. Expression of Activated Ras in Gastric Chief Cells of Mice Leads to the Full Spectrum of Metaplastic Lineage Transitions. Gastroenterology. 2016;150:918-30.e13 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 5a	Scalia C, Gendusa R, Cattoretti G. A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection. Appl Immunohistochem Mol Morphol. 2016;24:436-46 pubmed publisher
immunohistochemistry; human; 1:200	Balla P, Maros M, Barna G, Antal I, Papp G, Sapi Z, et al. Prognostic impact of reduced connexin43 expression and gap junction coupling of neoplastic stromal cells in giant cell tumor of bone. PLoS ONE. 2015;10:e0125316 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Nakano Tahara M, Terao M, Nishioka M, Kitaba S, Murota H, Katayama I. T helper 2 polarization in senile erythroderma with elevated levels of TARC and IgE. Dermatology. 2015;230:62-9 pubmed publisher
western blot; human; 1:50	Mimiola E, Marini O, Perbellini O, Micheletti A, Vermi W, Lonardi S, et al. Rapid reconstitution of functionally active 6-sulfoLacNAc(+) dendritic cells (slanDCs) of donor origin following allogeneic haematopoietic stem cell transplant. Clin Exp Immunol. 2014;178:129-41 pubmed publisher
immunohistochemistry - paraffin section; rhesus macaque immunohistochemistry - paraffin section; African green monkey	Kaliyaperumal S, Watkins B, Sharma P, Furlan S, Ramakrishnan S, Giver C, et al. CD8-predominant T-cell CNS infiltration accompanies GVHD in primates and is improved with immunoprophylaxis. Blood. 2014;123:1967-9 pubmed publisher
immunohistochemistry - paraffin section; human; 1:250	Klein J, Nguyen T, Bien Willner G, Chen L, Foyil K, Bartlett N, et al. CD163 immunohistochemistry is superior to CD68 in predicting outcome in classical Hodgkin lymphoma. Am J Clin Pathol. 2014;141:381-7 pubmed publisher
immunohistochemistry; human	Lorenzi L, Tabellini G, Vermi W, Moratto D, Porta F, Notarangelo L, et al. Occurrence of nodular lymphocyte-predominant hodgkin lymphoma in hermansky-pudlak type 2 syndrome is associated to natural killer and natural killer T cell defects. PLoS ONE. 2013;8:e80131 pubmed publisher
immunohistochemistry; human	Yoshida N, Oda M, Kuroda Y, Katayama Y, Okikawa Y, Masunari T, et al. Clinical significance of sIL-2R levels in B-cell lymphomas. PLoS ONE. 2013;8:e78730 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; tbl 2	Qiu S, Wei X, Huang W, Wu M, Qin Y, Li Y, et al. Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma. Sci Rep. 2013;3:2529 pubmed publisher
immunohistochemistry; human	Pagano L, Valentini C, Pulsoni A, Fisogni S, Carluccio P, Mannelli F, et al. Blastic plasmacytoid dendritic cell neoplasm with leukemic presentation: an Italian multicenter study. Haematologica. 2013;98:239-46 pubmed publisher
immunohistochemistry; human	Smith K, Pearson C, Hachey A, Xia D, Wachtman L. Alternative activation of macrophages in rhesus macaques (Macaca mulatta) with endometriosis. Comp Med. 2012;62:303-10 pubmed
immunohistochemistry; human; 1:100	Romeo S, Bovee J, Kroon H, Tirabosco R, Natali C, Zanatta L, et al. Malignant fibrous histiocytoma and fibrosarcoma of bone: a re-assessment in the light of currently employed morphological, immunohistochemical and molecular approaches. Virchows Arch. 2012;461:561-70 pubmed publisher
immunohistochemistry; human	Fung K, Chakrabarty J, Kern W, Magharyous H, Gehrs B, Li S. Intravascular large B-cell lymphoma with hemophagocytic syndrome (Asian variant) in a Caucasian patient. Int J Clin Exp Pathol. 2012;5:448-54 pubmed
immunohistochemistry; human; 1:50	Asano S, Mori K, Yamazaki K, Sata T, Kanno T, Sato Y, et al. Temporal differences of onset between primary skin lesions and regional lymph node lesions for tularemia in Japan: a clinicopathologic and immunohistochemical study of 19 skin cases and 54 lymph node cases. Virchows Arch. 2012;460:651-8 pubmed publisher
immunohistochemistry; human; 1:40	Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, et al. Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers. BMC Cancer. 2012;12:134 pubmed publisher
immunohistochemistry; rhesus macaque; 1:200	Macri S, Bailey C, de Oca N, Silva N, Rosene D, Mansfield K, et al. Immunophenotypic alterations in resident immune cells and myocardial fibrosis in the aging rhesus macaque (Macaca mulatta) heart. Toxicol Pathol. 2012;40:637-46 pubmed publisher
immunohistochemistry; human	Amador Ortiz C, Hurley M, Ghahramani G, Frisch S, Klco J, Lind A, et al. Use of classic and novel immunohistochemical markers in the diagnosis of cutaneous myeloid sarcoma. J Cutan Pathol. 2011;38:945-53 pubmed publisher
immunohistochemistry; human	Munari F, Lonardi S, Cassatella M, Doglioni C, Cangi M, Amedei A, et al. Tumor-associated macrophages as major source of APRIL in gastric MALT lymphoma. Blood. 2011;117:6612-6 pubmed publisher
immunohistochemistry; human; 1:100	Thakral B, Gleason B, Thomas A, Billings S, Victor T, Cibull T. Cellular neurothekeoma with fascicular growth features mimicking cellular dermatofibroma. Am J Dermatopathol. 2011;33:281-4 pubmed publisher
immunohistochemistry; human	Dettmer M, Hegemann I, Hegemann S. Extraintestinal Crohn's disease mimicking autoimmune inner ear disease: a histopathological approach. Audiol Neurootol. 2011;16:36-40 pubmed publisher
immunohistochemistry; human; 1:50	Poliani P, Kisand K, Marrella V, Ravanini M, Notarangelo L, Villa A, et al. Human peripheral lymphoid tissues contain autoimmune regulator-expressing dendritic cells. Am J Pathol. 2010;176:1104-12 pubmed publisher
immunohistochemistry; human	Heiland G, Aigner E, Dallos T, Sahinbegovic E, Krenn V, Thaler C, et al. Synovial immunopathology in haemochromatosis arthropathy. Ann Rheum Dis. 2010;69:1214-9 pubmed publisher
immunohistochemistry; human	Imanguli M, Swaim W, League S, Gress R, Pavletic S, Hakim F. Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa. Blood. 2009;113:3620-30 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Riener M, Wild P, Soll C, Knuth A, Jin B, Jungbluth A, et al. Frequent expression of the novel cancer testis antigen MAGE-C2/CT-10 in hepatocellular carcinoma. Int J Cancer. 2009;124:352-7 pubmed publisher
immunohistochemistry; human; 1:100	Ehses J, Perren A, Eppler E, Ribaux P, Pospisilik J, Maor Cahn R, et al. Increased number of islet-associated macrophages in type 2 diabetes. Diabetes. 2007;56:2356-70 pubmed
immunohistochemistry; rhesus macaque	Yearley J, Pearson C, Shannon R, Mansfield K. Phenotypic variation in myocardial macrophage populations suggests a role for macrophage activation in SIV-associated cardiac disease. AIDS Res Hum Retroviruses. 2007;23:515-24 pubmed
immunohistochemistry; human; 1:100	Schaer D, Schaer C, Schoedon G, Imhof A, Kurrer M. Hemophagocytic macrophages constitute a major compartment of heme oxygenase expression in sepsis. Eur J Haematol. 2006;77:432-6 pubmed
	Zwart E, van Ee T, Doppenberg D, Farina A, Wilmink J, Versteijne E, et al. The immune microenvironment after neoadjuvant therapy compared to upfront surgery in patients with pancreatic cancer. J Cancer Res Clin Oncol. 2023;149:14731-14743 pubmed publisher
	Yang F, Zeng Z, Li Y, Zhang D, Wei F, Zhao H, et al. The prognostic value of a 4-factor neoimmunologic score system in non-small cell lung cancer. J Leukoc Biol. 2022;112:1605-1619 pubmed publisher
	Noh J, Lee I, Han N, Kim M, Min Y, Lee S, et al. Additive Effect of CD73 Inhibitor in Colorectal Cancer Treatment With CDK4/6 Inhibitor Through Regulation of PD-L1. Cell Mol Gastroenterol Hepatol. 2022;14:769-788 pubmed publisher
	Sundaram S, Kim E, Jones G, Sivagnanam S, Tripathi M, Miremadi A, et al. Deciphering the Immune Complexity in Esophageal Adenocarcinoma and Pre-Cancerous Lesions With Sequential Multiplex Immunohistochemistry and Sparse Subspace Clustering Approach. Front Immunol. 2022;13:874255 pubmed publisher
	Johnson B, Creason A, Stommel J, Keck J, Parmar S, Betts C, et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med. 2022;3:100525 pubmed publisher
	Percivalle E, Sammartino J, Cassaniti I, Arbustini E, Urtis M, Smirnova A, et al. Macrophages and Monocytes: "Trojan Horses" in COVID-19. Viruses. 2021;13: pubmed publisher
	Gaibazzi N, Tuttolomondo D, Nicolini F, Tafuni A, Sartorio D, Martini C, et al. The Histopathological Correlate of Peri-Vascular Adipose Tissue Attenuation on Computed Tomography in Surgical Ascending Aorta Aneurysms: Is This a Measure of Tissue Inflammation?. Diagnostics (Basel). 2021;11: pubmed publisher
	Aid M, Busman Sahay K, Vidal S, Maliga Z, Bondoc S, Starke C, et al. Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques. Cell. 2020;183:1354-1366.e13 pubmed publisher
	Cho S, Oh Y, Jeong E, Park S, Lee D, Wang X, et al. Amplification of transglutaminase 2 enhances tumor-promoting inflammation in gastric cancers. Exp Mol Med. 2020;52:854-864 pubmed publisher
	Prencipe G, Bracaglia C, Caiello I, Pascarella A, Francalanci P, Pardeo M, et al. The interferon-gamma pathway is selectively up-regulated in the liver of patients with secondary hemophagocytic lymphohistiocytosis. PLoS ONE. 2019;14:e0226043 pubmed publisher
	Mattiola I, Tomay F, De Pizzol M, Silva Gomes R, Savino B, Gulic T, et al. The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell-mediated resistance to metastasis. Nat Immunol. 2019;20:1012-1022 pubmed publisher
	Reddy S, Reuben A, Barua S, Jiang H, Zhang S, Wang L, et al. Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer. Cancer Immunol Res. 2019;7:1025-1035 pubmed publisher
	Parker G, Li N, Takayama K, Farese A, MacVittie T. Lung and Heart Injury in a Nonhuman Primate Model of Partial-body Irradiation with Minimal Bone Marrow Sparing: Histopathological Evidence of Lung and Heart Injury. Health Phys. 2019;116:383-400 pubmed publisher
	Li W, Gorantla S, Gendelman H, Poluektova L. Systemic HIV-1 infection produces a unique glial footprint in humanized mouse brains. Dis Model Mech. 2017;10:1489-1502 pubmed publisher
	Young H, Rowling E, Bugatti M, Giurisato E, Luheshi N, Arozarena I, et al. An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition. J Exp Med. 2017;214:1691-1710 pubmed publisher
	Sauter J, Ambaye A, Mount S. Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital. Diagn Cytopathol. 2015;43:688-95 pubmed publisher
	Peroni A, Colato C, Schena D, Rongioletti F, Girolomoni G. Histiocytoid Sweet syndrome is infiltrated predominantly by M2-like macrophages. J Am Acad Dermatol. 2015;72:131-9 pubmed publisher
	Balla P, Moskovszky L, Sapi Z, Forsyth R, Knowles H, Athanasou N, et al. Epidermal growth factor receptor signalling contributes to osteoblastic stromal cell proliferation, osteoclastogenesis and disease progression in giant cell tumour of bone. Histopathology. 2011;59:376-89 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD163 Monoclonal Antibody (10D6)

Catalog # :

MA5-11458

Quantity :

500 µL

Price :

US 450.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 1:25-1:50, Western Blot: 1:100

Species :

Human

Clone :

10D6

Isotype :

IgG1

Storage :

4° C

Description :

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

Immunogen :

Recombinant protein encoding of domains 1-4 of the N-terminal region of human CD163

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:25-1:50, Western Blot: 1:100

Aliases :

CD163; CD163 antigen; CD163 molecule; CD163v2; CD163v3; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; putative CD163 antigen; SCARI1; Scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163; Soluble sCD163