Rb Monoclonal Antibody (1F8 (Rb1)) | Invitrogen MA5-11387 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Rb Monoclonal Antibody (1F8 (Rb1))

catalog :

MA5-11387

quantity :

500 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F8 (Rb1)

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:50; loading ...; fig 8a	Fomicheva M, Macara I. Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation. elife. 2020;9: pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Wang T, Goodman M, McGough R, Weiss K, Rao U. Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma. Int J Surg Pathol. 2014;22:589-99 pubmed publisher
immunohistochemistry; human; 1:200	Sahi H, Savola S, Sihto H, Koljonen V, Bohling T, Knuutila S. RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup. APMIS. 2014;122:1157-66 pubmed publisher
immunohistochemistry; human	Yanagawa N, Wang A, Kohler D, Santos G, Sykes J, Xu J, et al. Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients. Lung Cancer. 2013;79:215-20 pubmed publisher
immunohistochemistry; human; 1:50	Franchi A, Baroni G, Sardi I, Giunti L, Capanna R, Campanacci D. Dedifferentiated peripheral chondrosarcoma: a clinicopathologic, immunohistochemical, and molecular analysis of four cases. Virchows Arch. 2012;460:335-42 pubmed publisher
immunohistochemistry; human	Canzonieri V, Barzan L, Franchin G, Vaccher E, Talamini R, Sulfaro S, et al. Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas. J Cell Physiol. 2012;227:233-8 pubmed publisher
western blot; human; 1:200	Lee S, Liu S, Mitchell R, Slagle Webb B, Hong Y, Sheehan J, et al. HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A. Int J Cancer. 2011;129:2104-14 pubmed publisher
immunohistochemistry; human; 1:50	Panelos J, Beltrami G, Scoccianti G, Capanna R, Paglierani M, Pepi M, et al. Prognostic significance of the alterations of the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue. Ann Surg Oncol. 2011;18:566-71 pubmed publisher
immunocytochemistry; human; 1:200 immunohistochemistry; human western blot; human; 1:200	Derenzini M, Donati G, Mazzini G, Montanaro L, Vici M, Ceccarelli C, et al. Loss of retinoblastoma tumor suppressor protein makes human breast cancer cells more sensitive to antimetabolite exposure. Clin Cancer Res. 2008;14:2199-209 pubmed publisher
immunohistochemistry; human; 1:200	Jung C, Jung J, Lee K, Kang C, Kim M, Ko Y, et al. Centrosome abnormalities in non-small cell lung cancer: correlations with DNA aneuploidy and expression of cell cycle regulatory proteins. Pathol Res Pract. 2007;203:839-47 pubmed
immunocytochemistry; human; 1:200 western blot; human; 1:200	Montanaro L, Mazzini G, Barbieri S, Vici M, Nardi Pantoli A, Govoni M, et al. Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines. Cell Prolif. 2007;40:532-49 pubmed
western blot; mouse	Kim J, Hong S, Lee B, Hong J, Kwak J, Cho S, et al. The inhibition of T-cells proliferation by mouse mesenchymal stem cells through the induction of p16INK4A-cyclin D1/cdk4 and p21waf1, p27kip1-cyclin E/cdk2 pathways. Cell Immunol. 2007;245:16-23 pubmed
western blot; human	Choi J, Murillo G, Su B, Pezzuto J, Kinghorn A, Mehta R. Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells. FEBS J. 2006;273:5714-23 pubmed
immunohistochemistry; human	Nam E, Kim J, Kim S, Kim Y, Kim J, Yoon B, et al. The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance. Gynecol Oncol. 2007;104:207-11 pubmed
immunohistochemistry; human; 1:25	Yurakh A, Ramos D, Calabuig Fariñas S, Lopez Guerrero J, Rubio J, Solsona E, et al. Molecular and immunohistochemical analysis of the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder. Eur Urol. 2006;50:506-15; discussion 515 pubmed
western blot; rat	Kuo H, Kuo W, Lee Y, Lin W, Chou F, Tseng T. Inhibitory effect of caffeic acid phenethyl ester on the growth of C6 glioma cells in vitro and in vivo. Cancer Lett. 2006;234:199-208 pubmed
	Wadosky K, Berthiaume J, Tang W, Zungu M, Portman M, Gerdes A, et al. MuRF1 mono-ubiquitinates TRÎ± to inhibit T3-induced cardiac hypertrophy in vivo. J Mol Endocrinol. 2016;56:273-90 pubmed publisher
	Trere D, Ceccarelli C, Montanaro L, Tosti E, Derenzini M. Nucleolar size and activity are related to pRb and p53 status in human breast cancer. J Histochem Cytochem. 2004;52:1601-7 pubmed
	Derenzini M, Ceccarelli C, Santini D, Taffurelli M, Trere D. The prognostic value of the AgNOR parameter in human breast cancer depends on the pRb and p53 status. J Clin Pathol. 2004;57:755-61 pubmed
	Tzai T, Tsai Y, Chow N. The prevalence and clinicopathologic correlate of p16INK4a, retinoblastoma and p53 immunoreactivity in locally advanced urinary bladder cancer. Urol Oncol. 2004;22:112-8 pubmed
	Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari K, Abraham T, et al. p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma. Int J Cancer. 2004;109:710-6 pubmed
	Jung C, Motwani M, Schwartz G. Flavopiridol increases sensitization to gemcitabine in human gastrointestinal cancer cell lines and correlates with down-regulation of ribonucleotide reductase M2 subunit. Clin Cancer Res. 2001;7:2527-36 pubmed
	Esteller M, Cordon Cardo C, Corn P, Meltzer S, Pohar K, Watkins D, et al. p14ARF silencing by promoter hypermethylation mediates abnormal intracellular localization of MDM2. Cancer Res. 2001;61:2816-21 pubmed
	Huschtscha L, Noble J, Neumann A, Moy E, Barry P, Melki J, et al. Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells. Cancer Res. 1998;58:3508-12 pubmed

product information

Product Type :

Antibody

Product Name :

Rb Monoclonal Antibody (1F8 (Rb1))

Catalog # :

MA5-11387

Quantity :

500 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 1-2 ug/mL, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Species :

Human, Mouse

Clone :

1F8 (Rb1)

Isotype :

IgG1

Storage :

4 C

Description :

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Immunogen :

Recombinant human Rb protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 1-2 ug/mL, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Aliases :

Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p104; p104 chicken Rb; P105RB; p105-Rb; Phospho-Retinoblastoma; pp105; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; rb; RB transcriptional corepressor 1; RB1; Rb-1; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma 1 (including osteosarcoma); retinoblastoma 1 protein; retinoblastoma gene; retinoblastoma isoform A; retinoblastoma protein; retinoblastoma suspectibility protein; retinoblastoma tumor suppressor; retinoblastoma-associated protein

more info or order :

Invitrogen product webpage