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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Cytokeratin 15 Monoclonal Antibody (LHK15)
catalog :
MA5-11344
quantity :
500 uL
price :
US 408.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
LHK15
reactivity :
Saccharomycetales, human, mouse, rat, dogs, bovine, domestic sheep
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 54
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 8a
Gesteira T, Sun M, Coulson Thomas Y, Yamaguchi Y, Yeh L, Hascall V, et al. Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation. Invest Ophthalmol Vis Sci. 2017;58:4407-4421 pubmed publisher
  • immunohistochemistry; dogs; 1:50; fig 5d,6d,7d,8d
Watanabe K, Chambers J, Uchida K, Nibe K, Ushio N, Horiuchi N, et al. A cutaneous mixed tumor in a dog. J Vet Med Sci. 2017;79:670-673 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2c
Shin H, Yoon J, Koh W, Kim J, Kim C, Han K, et al. Nonpigmented hair removal using photodynamic therapy in animal model. Lasers Surg Med. 2016;48:748-762 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 3c
Mikhailova A, Ilmarinen T, Ratnayake A, Petrovski G, Uusitalo H, Skottman H, et al. Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction. Exp Eye Res. 2016;146:26-34 pubmed publisher
  • immunocytochemistry; human; 1:25; fig 2a
Fridriksdottir A, Kim J, Villadsen R, Klitgaard M, Hopkinson B, Petersen O, et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun. 2015;6:8786 pubmed publisher
  • immunocytochemistry; human; 1:25
  • immunohistochemistry; human; 1:25
Balk Møller E, Kim J, Hopkinson B, Timmermans Wielenga V, Petersen O, Villadsen R. A marker of endocrine receptor-positive cells, CEACAM6, is shared by two major classes of breast cancer: luminal and HER2-enriched. Am J Pathol. 2014;184:1198-208 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 3
Leung Y, Kandyba E, Chen Y, Ruffins S, Kobielak K. Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland. PLoS ONE. 2013;8:e74174 pubmed publisher
  • immunohistochemistry; mouse
Hughes M, Jiang T, Lin S, Leung Y, Kobielak K, Widelitz R, et al. Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis. J Invest Dermatol. 2014;134:24-32 pubmed publisher
  • immunocytochemistry; mouse; 1:1500
Petersson M, Frances D, Niemann C. Lineage tracing of hair follicle stem cells in epidermal whole mounts. Methods Mol Biol. 2013;989:45-60 pubmed publisher
  • immunohistochemistry; human; 1:250
Cayirli M, Kose O, Demiriz M. Clinical, dermoscopic and immunohistochemical assessment of actinic keratoses and evaluation of the effectiveness of diclofenac therapy with immunohistochemical analysis. Arch Dermatol Res. 2013;305:389-95 pubmed publisher
  • immunohistochemistry; human; 1:100
Higa K, Kato N, Yoshida S, Ogawa Y, Shimazaki J, Tsubota K, et al. Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium. Stem Cell Res. 2013;10:147-55 pubmed publisher
  • flow cytometry; human; 1:100
Fogarty S, Patel I, Trevisan J, Nakamura T, Hirschmugl C, Fullwood N, et al. Sub-cellular spectrochemical imaging of isolated human corneal cells employing synchrotron radiation-based Fourier-transform infrared microspectroscopy. Analyst. 2013;138:240-8 pubmed publisher
  • immunohistochemistry; human; 1:100
Wang X, Shi Y, Zhou Q, Liu X, Xu S, Lei T. Detailed histological structure of human hair follicle bulge region at different ages: a visible niche for nesting adult stem cells. J Huazhong Univ Sci Technolog Med Sci. 2012;32:648-656 pubmed publisher
  • immunohistochemistry; mouse; 1:100
García Zaragoza E, Pérez Tavarez R, Ballester A, Lafarga V, Jiménez Reinoso A, Ramirez A, et al. Intraepithelial paracrine Hedgehog signaling induces the expansion of ciliated cells that express diverse progenitor cell markers in the basal epithelium of the mouse mammary gland. Dev Biol. 2012;372:28-44 pubmed publisher
  • immunohistochemistry; mouse
Costa C, Santos M, Martinez Fernandez M, Duenas M, Lorz C, García Escudero R, et al. E2F1 loss induces spontaneous tumour development in Rb-deficient epidermis. Oncogene. 2013;32:2937-51 pubmed publisher
  • immunohistochemistry; mouse
Garza L, Liu Y, Yang Z, Alagesan B, Lawson J, Norberg S, et al. Prostaglandin D2 inhibits hair growth and is elevated in bald scalp of men with androgenetic alopecia. Sci Transl Med. 2012;4:126ra34 pubmed publisher
  • immunohistochemistry; human; 1:250
Mahalingam M, Richards J, Selim M, Muzikansky A, Hoang M. An immunohistochemical comparison of cytokeratin 7, cytokeratin 15, cytokeratin 19, CAM 5.2, carcinoembryonic antigen, and nestin in differentiating porocarcinoma from squamous cell carcinoma. Hum Pathol. 2012;43:1265-72 pubmed publisher
  • immunohistochemistry; human; 1:100
Kuo T, Chen C, Baron D, Onder T, Loewer S, Almeida S, et al. Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity. Nat Cell Biol. 2011;13:1214-23 pubmed publisher
  • immunohistochemistry; mouse; 1:50
Haffner M, Chaux A, Meeker A, Esopi D, Gerber J, Pellakuru L, et al. Global 5-hydroxymethylcytosine content is significantly reduced in tissue stem/progenitor cell compartments and in human cancers. Oncotarget. 2011;2:627-37 pubmed
  • immunohistochemistry; human; 1:150; tbl 3
Chui J, Coroneo M, Tat L, Crouch R, Wakefield D, Di Girolamo N. Ophthalmic pterygium: a stem cell disorder with premalignant features. Am J Pathol. 2011;178:817-27 pubmed publisher
  • immunohistochemistry; human
Garza L, Yang C, Zhao T, Blatt H, Lee M, He H, et al. Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells. J Clin Invest. 2011;121:613-22 pubmed publisher
  • immunohistochemistry; human
Qi H, Zheng X, Yuan X, Pflugfelder S, Li D. Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium. J Cell Physiol. 2010;225:180-5 pubmed publisher
  • immunohistochemistry; mouse
Lorz C, García Escudero R, Segrelles C, Garin M, Ariza J, Santos M, et al. A functional role of RB-dependent pathway in the control of quiescence in adult epidermal stem cells revealed by genomic profiling. Stem Cell Rev. 2010;6:162-77 pubmed publisher
  • immunohistochemistry; human
Laga A, Lai C, Zhan Q, Huang S, Velazquez E, Yang Q, et al. Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology. Am J Pathol. 2010;176:903-13 pubmed publisher
  • immunohistochemistry; human; 1:100
  • immunohistochemistry; mouse; 1:100
Amoh Y, Hoffman R. Isolation and culture of hair follicle pluripotent stem (hfPS) cells and their use for nerve and spinal cord regeneration. Methods Mol Biol. 2010;585:401-20 pubmed publisher
  • immunocytochemistry; human
Guo A, Jahoda C. An improved method of human keratinocyte culture from skin explants: cell expansion is linked to markers of activated progenitor cells. Exp Dermatol. 2009;18:720-6 pubmed publisher
  • immunocytochemistry; human; 1:100
Amoh Y, Kanoh M, Niiyama S, Hamada Y, Kawahara K, Sato Y, et al. Human hair follicle pluripotent stem (hfPS) cells promote regeneration of peripheral-nerve injury: an advantageous alternative to ES and iPS cells. J Cell Biochem. 2009;107:1016-20 pubmed publisher
  • flow cytometry; human; 1:300
  • immunohistochemistry; human; 1:300
Inoue K, Aoi N, Sato T, Yamauchi Y, Suga H, Eto H, et al. Differential expression of stem-cell-associated markers in human hair follicle epithelial cells. Lab Invest. 2009;89:844-56 pubmed publisher
  • immunocytochemistry; human
  • immunohistochemistry; human
  • western blot; human
Celis J, Gromova I, Cabezon T, Gromov P, Shen T, Timmermans Wielenga V, et al. Identification of a subset of breast carcinomas characterized by expression of cytokeratin 15: relationship between CK15+ progenitor/amplified cells and pre-malignant lesions and invasive disease. Mol Oncol. 2007;1:321-49 pubmed publisher
  • immunohistochemistry; human
  • western blot; human; 1:500
Celis J, Moreira J, Gromova I, Cabezon T, Gromov P, Shen T, et al. Characterization of breast precancerous lesions and myoepithelial hyperplasia in sclerosing adenosis with apocrine metaplasia. Mol Oncol. 2007;1:97-119 pubmed publisher
  • immunocytochemistry; mouse; 1:50
Stout G, Blasco M. Genetic dissection of the mechanisms underlying telomere-associated diseases: impact of the TRF2 telomeric protein on mouse epidermal stem cells. Dis Model Mech. 2009;2:139-56 pubmed publisher
  • immunohistochemistry; human; 1:50
Choi C, Park H, Kim Y, Lee S, Cho K. Elastic fiber staining and cytokeratin 15 expression pattern in trichoepithelioma and basal cell carcinoma. J Dermatol. 2008;35:499-502 pubmed publisher
  • immunocytochemistry; human; 1:100
Kanoh M, Amoh Y, Sato Y, Katsuoka K. Expression of the hair stem cell-specific marker nestin in epidermal and follicular tumors. Eur J Dermatol. 2008;18:518-23 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Amoh Y, Li L, Katsuoka K, Hoffman R. Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function. Cell Cycle. 2008;7:1865-9 pubmed
  • immunohistochemistry; human; 1:80
Pozdnyakova O, Mahalingam M. Involvement of the bulge region in primary scarring alopecia. J Cutan Pathol. 2008;35:922-5 pubmed publisher
  • immunocytochemistry; domestic sheep; 1:100
Liu Y, Zhou H, Gao F. Isolation and identification of stem cells from adult cashmere goat skin. Int J Dermatol. 2008;47:551-6 pubmed publisher
  • immunocytochemistry; domestic sheep
Islam M, Zhou H. Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus. Eur J Dermatol. 2007;17:302-8 pubmed
  • immunohistochemistry; mouse; 1:50
Iida M, Ihara S, Matsuzaki T. Hair cycle-dependent changes of alkaline phosphatase activity in the mesenchyme and epithelium in mouse vibrissal follicles. Dev Growth Differ. 2007;49:185-95 pubmed
  • immunohistochemistry; Saccharomycetales; 1:40
  • immunohistochemistry; human; 1:40
Raposio E, Guida C, Baldelli I, Curto M, Fiocca R, Kunkl A, et al. Characterization of multipotent cells from human adult hair follicles. Toxicol In Vitro. 2007;21:320-3 pubmed
  • immunohistochemistry; human; 1:200
Murakami D, Yamato M, Nishida K, Ohki T, Takagi R, Yang J, et al. Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells. J Artif Organs. 2006;9:185-91 pubmed
  • immunohistochemistry; human
Körver J, van Duijnhoven M, Pasch M, Van Erp P, van de Kerkhof P. Assessment of epidermal subpopulations and proliferation in healthy skin, symptomless and lesional skin of spreading psoriasis. Br J Dermatol. 2006;155:688-94 pubmed
  • immunohistochemistry; dogs; 1:200
Murakami D, Yamato M, Nishida K, Ohki T, Takagi R, Yang J, et al. The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithelial cell sheets. Biomaterials. 2006;27:5518-23 pubmed
  • immunohistochemistry; human
van Duijnhoven M, Körver J, Vissers W, van Vlijmen Willems I, Pasch M, Van Erp P, et al. Effect of calcipotriol on epidermal cell populations in alefacept-treated psoriatic lesions. J Eur Acad Dermatol Venereol. 2006;20:27-33 pubmed
  • flow cytometry; human
Ohyama M, Terunuma A, Tock C, Radonovich M, Pise Masison C, Hopping S, et al. Characterization and isolation of stem cell-enriched human hair follicle bulge cells. J Clin Invest. 2006;116:249-60 pubmed
  • immunohistochemistry; mouse; 1:100
Amoh Y, Li L, Campillo R, Kawahara K, Katsuoka K, Penman S, et al. Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves. Proc Natl Acad Sci U S A. 2005;102:17734-8 pubmed
  • immunohistochemistry; mouse; 1:100
Amoh Y, Li L, Katsuoka K, Penman S, Hoffman R. Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proc Natl Acad Sci U S A. 2005;102:5530-4 pubmed
  • immunohistochemistry; human
van Duijnhoven M, van de Kerkhof P, Pasch M, Muys L, van Erp P. The combination of the Zenon labeling technique and microscopic image analysis to study cell populations in normal and psoriatic epidermis. J Cutan Pathol. 2005;32:212-9 pubmed
Lee H, Lee J, Hong S, Sunwoo J, Kim H, Kim E, et al. Transplantation of human corneal limbal epithelial cell sheet harvested on synthesized carboxymethyl cellulose and dopamine in a limbal stem cell deficiency. J Tissue Eng Regen Med. 2021;15:139-149 pubmed publisher
Fujita Y, Komatsu M, Lee S, Kushida Y, Nakayama Nishimura C, Matsumura W, et al. Intravenous Injection of Muse Cells as a Potential Therapeutic Approach for Epidermolysis Bullosa. J Invest Dermatol. 2021;141:198-202.e6 pubmed publisher
Chu S, Chou C, Huang H, Yen M, Hong H, Chao P, et al. Mechanical stretch induces hair regeneration through the alternative activation of macrophages. Nat Commun. 2019;10:1524 pubmed publisher
Shi J, Lv Z, Nie M, Lu W, Liu C, Tian Y, et al. Human nail stem cells are retained but hypofunctional during aging. J Mol Histol. 2018;49:303-316 pubmed publisher
Villadsen R, Fridriksdottir A, Rønnov Jessen L, Gudjonsson T, Rank F, Labarge M, et al. Evidence for a stem cell hierarchy in the adult human breast. J Cell Biol. 2007;177:87-101 pubmed
Langner C, Wegscheider B, Ratschek M, Schips L, Zigeuner R. Keratin immunohistochemistry in renal cell carcinoma subtypes and renal oncocytomas: a systematic analysis of 233 tumors. Virchows Arch. 2004;444:127-34 pubmed
Whitaker Menezes D, Jones S, Friedman T, Korngold R, Murphy G. An epithelial target site in experimental graft-versus-host disease and cytokine-mediated cytotoxicity is defined by cytokeratin 15 expression. Biol Blood Marrow Transplant. 2003;9:559-70 pubmed
product information
Product Type :
Antibody
Product Name :
Cytokeratin 15 Monoclonal Antibody (LHK15)
Catalog # :
MA5-11344
Quantity :
500 uL
Price :
US 408.00
Clonality :
Monoclonal
Purity :
Protein A/G
Host :
Mouse
Reactivity :
Bovine, Human, Mouse, Rat
Applications :
Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 1:100-1:500, Western Blot: 1:100-1:500
Species :
Bovine, Human, Mouse, Rat
Clone :
LHK15
Isotype :
IgG2a, kappa
Storage :
4 C
Description :
Cytokeratin 15 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21. 2.
Immunogen :
A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 1:100-1:500, Western Blot: 1:100-1:500
Aliases :
AI528832; C K1CO; CK15; CK-15; cytokeratin 15; cytokeratin-15; K15; K1CO; Ka15; keratin 15; keratin 15, type I; keratin complex 1, acidic, gene 15; keratin, type I cytoskeletal 15; Keratin-15; keratin-15, basic; keratin-15, beta; KRT1; Krt1-15; KRT15; krt15 {ECO:0000250; KRTB; type I cytoskeletal 15; type I keratin KA15; UniProtKB:P19012}
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA