Thrombospondin 1 Antibody Cocktail | Invitrogen MA5-11330 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Thrombospondin 1 Antibody Cocktail

catalog :

MA5-11330

quantity :

500 µL

price :

US 506.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D4.6, A6.1, MBC 200.1

reactivity :

human, mouse, rat, domestic sheep

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, blocking or activating experiments

citations: 83

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; fig 6	Durrans A, Gao D, Gupta R, Fischer K, Choi H, El Rayes T, et al. Identification of Reprogrammed Myeloid Cell Transcriptomes in NSCLC. PLoS ONE. 2015;10:e0129123 pubmed publisher
immunocytochemistry; human western blot; human; 1:5000	Ganguly R, Sahu S, Chavez R, Raman P. Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro. Am J Physiol Cell Physiol. 2015;308:C111-22 pubmed publisher
western blot; human	Talme T, Bergdahl E, Sundqvist K. Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1. Immunology. 2014;142:176-92 pubmed publisher
western blot; human	Rogers M, Novak K, Zurakowski D, Cryan L, Blois A, Lifshits E, et al. Spontaneous reversion of the angiogenic phenotype to a nonangiogenic and dormant state in human tumors. Mol Cancer Res. 2014;12:754-64 pubmed publisher
immunohistochemistry; rat	Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C. Long-term gene therapy with thrombospondin 2 inhibits TGF-? activation, inflammation and angiogenesis in chronic allograft nephropathy. PLoS ONE. 2013;8:e83846 pubmed publisher
western blot; rat; 1:200	Phamduong E, Rathore M, Crews N, D Angelo A, Leinweber A, Kappera P, et al. Acute and chronic mu opioids differentially regulate thrombospondins 1 and 2 isoforms in astrocytes. ACS Chem Neurosci. 2014;5:106-14 pubmed publisher
western blot; mouse	Morrison M, Palenski T, Jamali N, Sheibani N, Sorenson C. Modulation of vascular cell function by bim expression. Int J Cell Biol. 2013;2013:297537 pubmed publisher
western blot; mouse; 1:1000	Zhao Y, Wang S, Sorenson C, Teixeira L, Dubielzig R, Peters D, et al. Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress. Mol Cell Biol. 2013;33:4225-40 pubmed publisher
western blot; human	Bergström S, Bergdahl E, Sundqvist K. A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation. Immunology. 2013;140:441-55 pubmed publisher
western blot; mouse	Palenski T, Sorenson C, Sheibani N. Inflammatory cytokine-specific alterations in retinal endothelial cell function. Microvasc Res. 2013;89:57-69 pubmed publisher
western blot; mouse	Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-? signaling pathways. J Cell Sci. 2013;126:1392-405 pubmed publisher
western blot; human	Bienes Martínez R, Ordoñez A, Feijóo Cuaresma M, Corral Escariz M, Mateo G, STENINA O, et al. Autocrine stimulation of clear-cell renal carcinoma cell migration in hypoxia via HIF-independent suppression of thrombospondin-1. Sci Rep. 2012;2:788 pubmed publisher
ELISA; human; 1:500	Martin Manso G, Navarathna D, Galli S, Soto Pantoja D, Kuznetsova S, Tsokos M, et al. Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis. PLoS ONE. 2012;7:e48775 pubmed publisher
ELISA; rat; 1:150	Narmada B, Chia S, Tucker Kellogg L, Yu H. HGF regulates the activation of TGF-?1 in rat hepatocytes and hepatic stellate cells. J Cell Physiol. 2013;228:393-401 pubmed publisher
immunohistochemistry; human; 1:100	Alkim C, Sakiz D, Alkim H, Livaoglu A, Kendir T, Demirsoy H, et al. Thrombospondin-1 and VEGF in inflammatory bowel disease. Libyan J Med. 2012;7: pubmed publisher
western blot; mouse	Sheibani N, Morrison M, Gurel Z, Park S, Sorenson C. BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment. Am J Physiol Renal Physiol. 2012;302:F809-19 pubmed publisher
western blot; human; 1:400	Sundaram P, Hultine S, Smith L, Dews M, Fox J, Biyashev D, et al. p53-responsive miR-194 inhibits thrombospondin-1 and promotes angiogenesis in colon cancers. Cancer Res. 2011;71:7490-501 pubmed publisher
western blot; human	Dews M, Fox J, Hultine S, Sundaram P, Wang W, Liu Y, et al. The myc-miR-17~92 axis blunts TGF{beta} signaling and production of multiple TGF{beta}-dependent antiangiogenic factors. Cancer Res. 2010;70:8233-46 pubmed publisher
western blot; rat	Ikeda H, Miyatake M, Koshikawa N, Ochiai K, Yamada K, Kiss A, et al. Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation. J Biol Chem. 2010;285:38415-27 pubmed publisher
western blot; human; 1:250	Hohberg M, Knöchel J, Hoffmann C, Chlench S, Wunderlich W, Alter A, et al. Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries. J Cell Physiol. 2011;226:350-61 pubmed publisher
western blot; mouse	Grutzmacher C, Park S, Elmergreen T, Tang Y, Scheef E, Sheibani N, et al. Opposing effects of bim and bcl-2 on lung endothelial cell migration. Am J Physiol Lung Cell Mol Physiol. 2010;299:L607-20 pubmed publisher
western blot; human	Olsson A, Björk A, Vallon Christersson J, Isaacs J, Leanderson T. Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors. Mol Cancer. 2010;9:107 pubmed publisher
immunohistochemistry; human western blot; human	Haldar S, Lu Y, Jeyaraj D, Kawanami D, Cui Y, Eapen S, et al. Klf15 deficiency is a molecular link between heart failure and aortic aneurysm formation. Sci Transl Med. 2010;2:26ra26 pubmed publisher
western blot; human; 1:2000	Zaslavsky A, Baek K, Lynch R, Short S, Grillo J, Folkman J, et al. Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis. Blood. 2010;115:4605-13 pubmed publisher
western blot; human; 1:200	Hyder S, Liang Y, Wu J, Welbern V. Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells. Endocr Relat Cancer. 2009;16:809-17 pubmed publisher
blocking or activating experiments; mouse	Isenberg J, Shiva S, Gladwin M. Thrombospondin-1-CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO-cGMP pathway activation. Nitric Oxide. 2009;21:52-62 pubmed publisher
western blot; human; 1:200	Hyder S, Liang Y, Wu J. Estrogen regulation of thrombospondin-1 in human breast cancer cells. Int J Cancer. 2009;125:1045-53 pubmed publisher
western blot; human; 1:400	Scheef E, Sorenson C, Sheibani N. Attenuation of proliferation and migration of retinal pericytes in the absence of thrombospondin-1. Am J Physiol Cell Physiol. 2009;296:C724-34 pubmed publisher
western blot; mouse	Huang Q, Sheibani N. High glucose promotes retinal endothelial cell migration through activation of Src, PI3K/Akt1/eNOS, and ERKs. Am J Physiol Cell Physiol. 2008;295:C1647-57 pubmed publisher
blocking or activating experiments; human	Gayen Betal S, Setty B. Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain. Transl Res. 2008;152:165-77 pubmed publisher
immunohistochemistry; mouse; 1:400	Johnson A, O Connell M, Miyamoto H, Huang J, Yao J, Messing E, et al. Androgenic dependence of exophytic tumor growth in a transgenic mouse model of bladder cancer: a role for thrombospondin-1. BMC Urol. 2008;8:7 pubmed publisher
immunohistochemistry; human; 1:2000 western blot; human	Rojas A, Meherem S, Kim Y, Washington M, Willis J, Markowitz S, et al. The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer. Int J Cancer. 2008;123:14-21 pubmed publisher
western blot; mouse	Kondo S, Tang Y, Scheef E, Sheibani N, Sorenson C. Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2. Am J Physiol Cell Physiol. 2008;294:C1521-30 pubmed publisher
western blot; human	Moon Y, Kim J, Yang H, Eling T. Growth compensatory role of sulindac sulfide-induced thrombospondin-1 linked with ERK1/2 and RhoA GTPase signaling pathways. Life Sci. 2008;82:591-9 pubmed publisher
ELISA; mouse	Calzada M, Kuznetsova S, Sipes J, Rodrigues R, Cashel J, Annis D, et al. Calcium indirectly regulates immunochemical reactivity and functional activities of the N-domain of thrombospondin-1. Matrix Biol. 2008;27:339-51 pubmed publisher
western blot; human	Park J, Lee H, Ko K, Park S, Kim J, Choe G, et al. Meteorin regulates angiogenesis at the gliovascular interface. Glia. 2008;56:247-58 pubmed
western blot; mouse	Scheef E, Huang Q, Wang S, Sorenson C, Sheibani N. Isolation and characterization of corneal endothelial cells from wild type and thrombospondin-1 deficient mice. Mol Vis. 2007;13:1483-95 pubmed
blocking or activating experiments; human	Derks R, Jankowska Gan E, Xu Q, Burlingham W. Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1. J Immunol. 2007;179:3443-51 pubmed
blocking or activating experiments; human; 1:1000	Hong S, Chang S, Yeom D, Kang J, Hong K. Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A. Anticancer Drugs. 2007;18:1005-14 pubmed
immunohistochemistry; domestic sheep; 1:50	Sozo F, Hooper S, Wallace M. Thrombospondin-1 expression and localization in the developing ovine lung. J Physiol. 2007;584:625-35 pubmed
ELISA; human	Annis D, Gunderson K, Mosher D. Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations. J Biol Chem. 2007;282:27067-75 pubmed
western blot; mouse; 1:3000	Kondo S, Scheef E, Sheibani N, Sorenson C. PECAM-1 isoform-specific regulation of kidney endothelial cell migration and capillary morphogenesis. Am J Physiol Cell Physiol. 2007;292:C2070-83 pubmed
immunohistochemistry; rat	de Nigris F, Balestrieri M, Williams Ignarro S, D Armiento F, Fiorito C, Ignarro L, et al. The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats. Nitric Oxide. 2007;17:50-4 pubmed
western blot; mouse	Kaipainen A, Kieran M, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, et al. PPARalpha deficiency in inflammatory cells suppresses tumor growth. PLoS ONE. 2007;2:e260 pubmed
western blot; mouse; 1:4000	Sheibani N, Scheef E, Dimaio T, Wang Y, Kondo S, Sorenson C. Bcl-2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells. J Cell Physiol. 2007;210:616-25 pubmed
immunohistochemistry; mouse; 1:25	Isenberg J, Hyodo F, Matsumoto K, Romeo M, Abu Asab M, Tsokos M, et al. Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide-mediated vascular smooth muscle relaxation. Blood. 2007;109:1945-52 pubmed
western blot; mouse; 1:1000	Giuriato S, Ryeom S, Fan A, Bachireddy P, Lynch R, Rioth M, et al. Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch. Proc Natl Acad Sci U S A. 2006;103:16266-71 pubmed
immunohistochemistry; human; 1:500	Duvic M, Talpur R, Ni X, Zhang C, Hazarika P, Kelly C, et al. Phase 2 trial of oral vorinostat (suberoylanilide hydroxamic acid, SAHA) for refractory cutaneous T-cell lymphoma (CTCL). Blood. 2007;109:31-9 pubmed
western blot; human	Hopkinson A, McIntosh R, Shanmuganathan V, Tighe P, Dua H. Proteomic analysis of amniotic membrane prepared for human transplantation: characterization of proteins and clinical implications. J Proteome Res. 2006;5:2226-35 pubmed
western blot; mouse	Dews M, Homayouni A, Yu D, Murphy D, Sevignani C, Wentzel E, et al. Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster. Nat Genet. 2006;38:1060-5 pubmed
immunohistochemistry; mouse; 1:50	Shoji T, Koyama H, Morioka T, Tanaka S, Kizu A, Motoyama K, et al. Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes. Diabetes. 2006;55:2245-55 pubmed
western blot; mouse	Li Z, Wang C, Jiao X, Lu Y, Fu M, Quong A, et al. Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling. Mol Cell Biol. 2006;26:4240-56 pubmed
immunohistochemistry; human; 1:50	Bodner Adler B, Nather A, Bodner K, Czerwenka K, Kimberger O, Leodolter S, et al. Expression of thrombospondin 1 (TSP 1) in patients with uterine smooth muscle tumors: an immunohistochemical study. Gynecol Oncol. 2006;103:186-9 pubmed
western blot; human; 1:750 immunocytochemistry; mouse; 1:250 western blot; mouse; 1:750	Connerney J, Andreeva V, Leshem Y, Muentener C, Mercado M, Spicer D. Twist1 dimer selection regulates cranial suture patterning and fusion. Dev Dyn. 2006;235:1345-57 pubmed
immunohistochemistry; human; 1,000 ug/ml western blot; human; 1:200	Jo W, Mizukami Y, Duerr E, Zukerberg L, Chung D. Wnt signaling can repress thrombospondin-1 expression in colonic tumorigenesis. Cancer Biol Ther. 2005;4:1361-6 pubmed
western blot; mouse; 1:100	Sezaki S, Hirohata S, Iwabu A, Nakamura K, Toeda K, Miyoshi T, et al. Thrombospondin-1 is induced in rat myocardial infarction and its induction is accelerated by ischemia/reperfusion. Exp Biol Med (Maywood). 2005;230:621-30 pubmed
western blot; mouse	Scheef E, Wang S, Sorenson C, Sheibani N. Isolation and characterization of murine retinal astrocytes. Mol Vis. 2005;11:613-24 pubmed
western blot; human	Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, et al. Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch. Am J Pathol. 2005;166:1883-94 pubmed
ELISA; human; 1:500	Isenberg J, Calzada M, Zhou L, Guo N, Lawler J, Wang X, et al. Endogenous thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor. Matrix Biol. 2005;24:110-23 pubmed
immunohistochemistry; mouse	Olson L, Zhang J, Taylor H, Rose D, Rosenfeld M. Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling. Proc Natl Acad Sci U S A. 2005;102:3708-13 pubmed
western blot; rat	Bouvet C, Gilbert L, Girardot D, Deblois D, Moreau P. Different involvement of extracellular matrix components in small and large arteries during chronic NO synthase inhibition. Hypertension. 2005;45:432-7 pubmed
immunoprecipitation; rat immunohistochemistry; rat western blot; rat	Ihara Y, Manabe S, Kanda M, Kawano H, Nakayama T, Sekine I, et al. Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats. Glycobiology. 2005;15:383-92 pubmed
	Marcheteau E, Farge T, P xe9 r xe8 s M, Labrousse G, Tenet J, Delmas S, et al. Thrombospondin-1 Silencing Improves Lymphocyte Infiltration in Tumors and Response to Anti-PD-1 in Triple-Negative Breast Cancer. Cancers (Basel). 2021;13: pubmed publisher
	Seif K, Alidzanovic L, Tischler B, Ibrahim N, Zagrapan B, Rauscher S, et al. Neutrophil-Mediated Proteolysis of Thrombospondin-1 Promotes Platelet Adhesion and String Formation. Thromb Haemost. 2018;118:2074-2085 pubmed publisher
	Li S, Forslöw A, Sundqvist K. Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction. J Immunol. 2005;174:654-61 pubmed
	Flügel Koch C, Ohlmann A, Fuchshofer R, Welge Lussen U, Tamm E. Thrombospondin-1 in the trabecular meshwork: localization in normal and glaucomatous eyes, and induction by TGF-beta1 and dexamethasone in vitro. Exp Eye Res. 2004;79:649-63 pubmed
	Emonard H, Bellon G, Troeberg L, Berton A, Robinet A, Henriet P, et al. Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism. J Biol Chem. 2004;279:54944-51 pubmed
	Brittain J, Han J, Ataga K, Orringer E, Parise L. Mechanism of CD47-induced alpha4beta1 integrin activation and adhesion in sickle reticulocytes. J Biol Chem. 2004;279:42393-402 pubmed
	Ziehr J, Sheibani N, Sorenson C. Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice. Am J Physiol Renal Physiol. 2004;287:F1154-63 pubmed
	Sengupta K, Banerjee S, Saxena N, Banerjee S. Thombospondin-1 disrupts estrogen-induced endothelial cell proliferation and migration and its expression is suppressed by estradiol. Mol Cancer Res. 2004;2:150-8 pubmed
	Liu X, Wu Z, Sheibani N, Brandt C, Polansky J, Kaufman P. Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells. Exp Eye Res. 2003;77:181-8 pubmed
	Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt Clermont P, Kovacic H. Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol Cell Biol. 2003;23:5401-8 pubmed
	Alaoui Jamali M, Song D, Benlimame N, Yen L, Deng X, Hernandez Perez M, et al. Regulation of multiple tumor microenvironment markers by overexpression of single or paired combinations of ErbB receptors. Cancer Res. 2003;63:3764-74 pubmed
	Su X, Sorenson C, Sheibani N. Isolation and characterization of murine retinal endothelial cells. Mol Vis. 2003;9:171-8 pubmed
	Rice A, Steward M, Quinn C. Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast. J Clin Pathol. 2002;55:921-5 pubmed
	Flügel Koch C, Ohlmann A, Piatigorsky J, Tamm E. Disruption of anterior segment development by TGF-beta1 overexpression in the eyes of transgenic mice. Dev Dyn. 2002;225:111-25 pubmed
	Mattern J, Koomägi R, Volm M. Characteristics of long-term survivors of untreated lung cancer. Lung Cancer. 2002;36:277-82 pubmed
	Li S, Ivanoff A, Bergström S, Sandström A, Christensson B, van Nerven J, et al. T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components. Eur J Immunol. 2002;32:1069-79 pubmed
	Zhang X, Riedel C, Carrasco N, Arvan P. Polarized trafficking of thyrocyte proteins in MDCK cells. Mol Cell Endocrinol. 2002;188:27-36 pubmed
	Kragh M, Quistorff B, Tenan M, Van Meir E, Kristjansen P. Overexpression of thrombospondin-1 reduces growth and vascular index but not perfusion in glioblastoma. Cancer Res. 2002;62:1191-5 pubmed
	Rofstad E, Graff B. Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts. J Invest Dermatol. 2001;117:1042-9 pubmed
	Shi S, Cote R, Hawes D, Thu S, Shi Y, Young L, et al. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?. J Histochem Cytochem. 1999;47:463-70 pubmed
	Gupta K, Gupta P, Solovey A, Hebbel R. Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin. Biochim Biophys Acta. 1999;1453:63-73 pubmed

product information

Product Type :

Antibody

Product Name :

Thrombospondin 1 Antibody Cocktail

Catalog # :

MA5-11330

Quantity :

500 µL

Price :

US 506.00

Clonality :

Cocktail

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Western Blot: 0.5-1.0 µg/mL

Species :

Human

Clone :

D4.6, A6.1, MBC 200.1

Isotype :

IgG1

Storage :

4° C

Description :

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Immunogen :

Purified human TSP

Format :

Liquid

Applications w/Dilutions :

Western Blot: 0.5-1.0 µg/mL

Aliases :

glycoprotein G; tbsp1; THBS; Thbs1; Thbs-1; thrombospondin; thrombospondin 1; thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1; TSP; TSP1; TSP-1