p53 Monoclonal Antibody (PAb 1801) | Invitrogen MA5-11296 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p53 Monoclonal Antibody (PAb 1801)

catalog :

MA5-11296

quantity :

500 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Pab 1801

reactivity :

African green monkey, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, other

more info or order :

Invitrogen product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
other; mouse; loading ...; fig 6q, 7q	Vessey K, Jobling A, Tran M, Wang A, Greferath U, Fletcher E. Treatments targeting autophagy ameliorate the age-related macular degeneration phenotype in mice lacking APOE (apolipoprotein E). Autophagy. 2022;18:2368-2384 pubmed publisher
western blot; human; 1:1000; fig 5	Shi Z, Shi F, Wang Y, Sheftel A, Nie G, Zhao Y, et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci. 2015;72:983-97 pubmed publisher
western blot; human; 1:800	Williams R, Barnhill L, Kuo H, Lin W, Batova A, Yu A, et al. Chimeras of p14ARF and p16: functional hybrids with the ability to arrest growth. PLoS ONE. 2014;9:e88219 pubmed publisher
immunocytochemistry; human; 1:100 western blot; human; 1:500	Hernández Bule M, Trillo M, Ubeda A. Molecular mechanisms underlying antiproliferative and differentiating responses of hepatocarcinoma cells to subthermal electric stimulation. PLoS ONE. 2014;9:e84636 pubmed publisher
western blot; human; 0.5 ug/ml	Sousa M, Zub K, Aas P, Hanssen Bauer A, Demirovic A, Sarno A, et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS ONE. 2013;8:e55493 pubmed publisher
western blot; human; 1:2000; fig 3	Frazzi R, Valli R, Tamagnini I, Casali B, Latruffe N, Merli F. Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation. Int J Cancer. 2013;132:1013-21 pubmed publisher
immunohistochemistry; human; 1:100	Fan C, Lin X, Wang E. Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study. Tumour Biol. 2012;33:1045-52 pubmed
immunocytochemistry; human; 1:100 immunohistochemistry; human; 1:100	Fan C, Zhao Y, Liu D, Zhang X, Wang E. Detection of Brk expression in non-small cell lung cancer: clinicopathological relevance. Tumour Biol. 2011;32:873-80 pubmed publisher
western blot; human; fig 1	Frazzi R, Casali B, Iori M, Nicoli D, Mammi C, Merli F. Increase in clusterin forms part of the stress response in Hodgkin's lymphoma. Int J Oncol. 2011;38:677-84 pubmed publisher
immunohistochemistry; human	Graziano S, Gu L, Wang X, Tatum A, Vollmer R, Strauss G, et al. Prognostic significance of mucin and p53 expression in stage IB non-small cell lung cancer: a laboratory companion study to CALGB 9633. J Thorac Oncol. 2010;5:810-7 pubmed
immunohistochemistry; human; 1:200	de Feraudy S, Boubakour Azzouz I, Fraitag S, Berneburg M, Chan L, Chew K, et al. Diagnosing xeroderma pigmentosum group C by immunohistochemistry. Am J Dermatopathol. 2010;32:109-17 pubmed publisher
western blot; human	Cai B, Chen J, Lu M, Chang L, Lin H, Chang Y, et al. Functional four-base A/T gap core sequence CATTAG of P53 response elements specifically bound tetrameric P53 differently than two-base A/T gap core sequence CATG bound both dimeric and tetrameric P53. Nucleic Acids Res. 2009;37:1984-90 pubmed publisher
chromatin immunoprecipitation; human immunoprecipitation; human; 1 ug/time western blot; human	Zhu Q, Wani G, Yao J, Patnaik S, Wang Q, El Mahdy M, et al. The ubiquitin-proteasome system regulates p53-mediated transcription at p21waf1 promoter. Oncogene. 2007;26:4199-208 pubmed
immunohistochemistry; human; 1:150	Mangia A, Chiriatti A, Ranieri G, Abbate I, Coviello M, Simone G, et al. H pylori status and angiogenesis factors in human gastric carcinoma. World J Gastroenterol. 2006;12:5465-72 pubmed
western blot; human; 1:2000; fig 5 western blot; African green monkey; 1:2000; fig 3	Kanezaki R, Toki T, Xu G, Narayanan R, Ito E. Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5. Tohoku J Exp Med. 2006;209:169-80 pubmed
western blot; human; 1:1000-1:5000	Heminger K, Jain V, Kadakia M, Dwarakanath B, Berberich S. Altered gene expression induced by ionizing radiation and glycolytic inhibitor 2-deoxy-glucose in a human glioma cell line: implications for radio sensitization. Cancer Biol Ther. 2006;5:815-23 pubmed
western blot; human	van Beusechem V, van den Doel P, Gerritsen W. Conditionally replicative adenovirus expressing degradation-resistant p53 for enhanced oncolysis of human cancer cells overexpressing murine double minute 2. Mol Cancer Ther. 2005;4:1013-8 pubmed
immunohistochemistry - paraffin section; mouse; fig 1	Elso C, Lu X, Culiat C, Rutledge J, Cacheiro N, Generoso W, et al. Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice. Hum Mol Genet. 2004;13:2813-21 pubmed
immunohistochemistry - paraffin section; human	Barshack I, Goldberg I, Chowers Y, Weiss B, Horowitz A, Kopolovic J. Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue. J Clin Pathol. 2001;54:684-8 pubmed
western blot; human	Evdokiou A, Atkins G, Bouralexis S, Hay S, Raggatt L, Cowled P, et al. Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone. Int J Oncol. 2001;19:625-32 pubmed
	Patwardhan G, Hosain S, Liu D, Khiste S, Zhao Y, Bielawski J, et al. Ceramide modulates pre-mRNA splicing to restore the expression of wild-type tumor suppressor p53 in deletion-mutant cancer cells. Biochim Biophys Acta. 2014;1841:1571-80 pubmed publisher
	Wunderlich M, Ghosh M, Weghorst K, Berberich S. MdmX represses E2F1 transactivation. Cell Cycle. 2004;3:472-8 pubmed
	Chandra J, Hackbarth J, Le S, Loegering D, Bone N, Bruzek L, et al. Involvement of reactive oxygen species in adaphostin-induced cytotoxicity in human leukemia cells. Blood. 2003;102:4512-9 pubmed
	Ricotti L, Tesei A, De Paola F, Ulivi P, Frassineti G, Milandri C, et al. In vitro schedule-dependent interaction between docetaxel and gemcitabine in human gastric cancer cell lines. Clin Cancer Res. 2003;9:900-5 pubmed
	Blajeski A, Phan V, Kottke T, Kaufmann S. G(1) and G(2) cell-cycle arrest following microtubule depolymerization in human breast cancer cells. J Clin Invest. 2002;110:91-9 pubmed
	Gajra A, Tatum A, Newman N, Gamble G, Lichtenstein S, Rooney M, et al. The predictive value of neuroendocrine markers and p53 for response to chemotherapy and survival in patients with advanced non-small cell lung cancer. Lung Cancer. 2002;36:159-65 pubmed
	Nielsen L, Gurnani M, Shi B, Terracina G, Johnson R, Carroll J, et al. Derivation and initial characterization of a mouse mammary tumor cell line carrying the polyomavirus middle T antigen: utility in the development of novel cancer therapeutics. Cancer Res. 2000;60:7066-74 pubmed
	Shinobu N, Maeda T, Aso T, Ito T, Kondo T, Koike K, et al. Physical interaction and functional antagonism between the RNA polymerase II elongation factor ELL and p53. J Biol Chem. 1999;274:17003-10 pubmed
	Boyer S, Wazer D, Band V. E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway. Cancer Res. 1996;56:4620-4 pubmed

product information

Product Type :

Antibody

Product Name :

p53 Monoclonal Antibody (PAb 1801)

Catalog # :

MA5-11296

Quantity :

500 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

ChIP Assay: 5 ug, Immunoprecipitation: 2 ug/mL, Western Blot: 0.5-2 ug/mL

Species :

Human

Clone :

PAb 1801

Isotype :

IgG1

Storage :

4 C

Description :

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

Immunogen :

Recombinant Human p53 protein

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 5 ug, Immunoprecipitation: 2 ug/mL, Western Blot: 0.5-2 ug/mL

Aliases :

Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3

more info or order :

Invitrogen product webpage