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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified
catalog :
MA5-11198
quantity :
100 µL
price :
US 496.00
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
G.299.10
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, other
more info or order :
Invitrogen product webpage
citations: 9
Published Application/Species/Sample/DilutionReference
  • other; human; 1:100; loading ...; fig s7a
Bakhoum M, Francis J, Agustinus A, Earlie E, Di Bona M, Abramson D, et al. Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression. Nat Commun. 2021;12:5402 pubmed publisher
Rafnsdottir S, Jang K, Halldorsdottir S, Tomasdottir A, Vinod M, M xf6 ller K, et al. SMYD5 is a novel epigenetic gatekeeper of the mild hypothermia response. bioRxiv. 2023;: pubmed publisher
Weigert R, Hetzel S, Bailly N, Haggerty C, Ilik I, Yung P, et al. Dynamic antagonism between key repressive pathways maintains the placental epigenome. Nat Cell Biol. 2023;25:579-591 pubmed publisher
Xu L, Zhang L, Xiang Y, Zhang X. Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome. Regen Ther. 2023;22:210-223 pubmed publisher
Chen W, Wang H, Ji J, WANG Z, Shi T, Wu M, et al. Epigenetic network of EZH2/SFRP1/Wnt in the epithelial-mesenchymal transition of laryngeal carcinoma cells. Neoplasma. 2022;69:680-690 pubmed publisher
Gopalan S, Fazzio T. Multi-CUT&Tag to simultaneously profile multiple chromatin factors. STAR Protoc. 2022;3:101100 pubmed publisher
Gopalan S, Wang Y, Harper N, Garber M, Fazzio T. Simultaneous profiling of multiple chromatin proteins in the same cells. Mol Cell. 2021;81:4736-4746.e5 pubmed publisher
Leske H, Dalgleish R, Lazar A, Reifenberger G, Cree I. A common classification framework for histone sequence alterations in tumours: an expert consensus proposal. J Pathol. 2021;254:109-120 pubmed publisher
Beuzelin D, Pitard B, Kaeffer B. Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat. Front Physiol. 2019;10:1037 pubmed publisher
product information
Product Type :
Antibody
Product Name :
H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified
Catalog # :
MA5-11198
Quantity :
100 µL
Price :
US 496.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse, Non-human primate, Rat
Applications :
ChIP Assay: 1-5 µg, ChIP-Sequencing: 3 µg, Immunocytochemistry: 1:1,600, Immunohistochemistry (Paraffin): 1:100-1:400, Peptide array: 1:2,000, Western Blot: 1:1,000
Species :
Human, Mouse, Non-human primate, Rat
Clone :
G.299.10
Isotype :
IgG
Storage :
-20°C
Description :
H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.
Immunogen :
Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 1-5 µg, ChIP-Sequencing: 3 µg, Immunocytochemistry: 1:1,600, Immunohistochemistry (Paraffin): 1:100-1:400, Peptide array: 1:2,000, Western Blot: 1:1,000
Aliases :
H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3B.1; H3 histone, family 3C; H3 K27; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3.3A; H3.3b; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3b; h3-5; H3-53; H3-614; H3a; H3b; H3-B; H3C1; H3C10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3c8; H3f; H3-F; H3F1K; H3F2; H3F3; h3f3a; H3f3b; h3f3b.1; H3F3C; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3k27; H3Lys27me3; h3r; Hist1; Hist1h3a; HIST1H3B; Hist1h3c; Hist1h3d; HIST1H3E; HIST1H3F; hist1h3g; hist1h3g.L; HIST1H3H; Hist1h3i; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H3a; histone cluster 1, H3b; histone cluster 1, H3f; histone cluster 1, H3g protein L homeolog; histone cluster 1, H3h; histone cluster 2, H3a; histone cluster 2, H3c; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; histone H3.1; Histone H3.2; histone H3.3; Histone H3.3C; histone H3.5; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; histone H3/l; Histone H3/m; histone H3/o; Histone H3K27me3; histone variant H3.5; hypothetical protein LOC550262; M32461; methyl Histone 3; methyl Histone H3; PP781; Trimethyl H3; Tri-methyl H3; Trimethyl Histone H3; Tri-methyl Histone H3; Tri-Methyl-H3 K27; Tri-Methyl-H3 Lys27; Tri-Methyl-Histone H3 K27; Tri-Methyl-Histone H3 Lys27; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; XELAEV_18028537mg; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA