Phosphotyrosine Monoclonal Antibody (IG2) | Invitrogen MA4-003 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phosphotyrosine Monoclonal Antibody (IG2)

catalog :

MA4-003

quantity :

100 ug

price :

US 451.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IG2

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 4

Reference
Nguyen M, Satoh H, Favelyukis S, Babendure J, Imamura T, Sbodio J, et al. JNK and tumor necrosis factor-alpha mediate free fatty acid-induced insulin resistance in 3T3-L1 adipocytes. J Biol Chem. 2005;280:35361-71 pubmed
Preis P, Waldman F, Frackelton A, Saya H, Levin V. Protein-tyrosine kinase activity and pp60v-src expression in whole cells measured by flow cytometry. Cancer Res. 1988;48:4633-8 pubmed
Huhn R, Posner M, Rayter S, Foulkes J, Frackelton A. Cell lines and peripheral blood leukocytes derived from individuals with chronic myelogenous leukemia display virtually identical proteins phosphorylated on tyrosine residues. Proc Natl Acad Sci U S A. 1987;84:4408-12 pubmed
Frackelton A, Posner M, Kannan B, Mermelstein F. Generation of monoclonal antibodies against phosphotyrosine and their use for affinity purification of phosphotyrosine-containing proteins. Methods Enzymol. 1991;201:79-92 pubmed

product information

Product Type :

Antibody

Product Name :

Phosphotyrosine Monoclonal Antibody (IG2)

Catalog # :

MA4-003

Quantity :

100 ug

Price :

US 451.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Chemical

Applications :

ELISA: 1 ug/mL, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 - 1:200, Immunoprecipitation: 2 ug, Western Blot: 1:100-1:1,000

Species :

Chemical

Clone :

IG2

Isotype :

IgG1

Storage :

4 C

Description :

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Immunogen :

O-phospho-L-tyrosine and O-phospho-DL-tyramine

Format :

Liquid

Applications w/Dilutions :

ELISA: 1 ug/mL, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 - 1:200, Immunoprecipitation: 2 ug, Western Blot: 1:100-1:1,000

Aliases :

Phosphotyrosine; pTyr; pY