CD31 Monoclonal Antibody (HEC7) | Invitrogen MA3100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD31 Monoclonal Antibody (HEC7)

catalog :

MA3100

quantity :

200 µg

price :

US 420.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HEC7

reactivity :

human, mouse, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s1-2	Bersini S, Schulte R, Huang L, Tsai H, Hetzer M. Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome. elife. 2020;9: pubmed publisher
immunocytochemistry; human; 1:100; loading ...; fig 4i	Wang X, Liu R, Zhu W, Chu H, Yu H, Wei P, et al. UDP-glucose accelerates SNAI1 mRNA decay and impairs lung cancer metastasis. Nature. 2019;571:127-131 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 2f	Lovric S, Gonçalves S, Gee H, Oskouian B, Srinivas H, Choi W, et al. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency. J Clin Invest. 2017;127:912-928 pubmed publisher
immunocytochemistry; human	Totonchy J, Osborn J, Botto S, Clepper L, Moses A. Aberrant proliferation in CXCR7+ endothelial cells via degradation of the retinoblastoma protein. PLoS ONE. 2013;8:e69828 pubmed publisher
flow cytometry; human	Alexaki V, Notas G, Pelekanou V, Kampa M, Valkanou M, Theodoropoulos P, et al. Adipocytes as immune cells: differential expression of TWEAK, BAFF, and APRIL and their receptors (Fn14, BAFF-R, TACI, and BCMA) at different stages of normal and pathological adipose tissue development. J Immunol. 2009;183:5948-56 pubmed publisher
blocking or activating experiments; human; 20 ug/ml	Williams M, Sakurai Y, Zughaier S, Eskin S, McIntire L. Transmigration across activated endothelium induces transcriptional changes, inhibits apoptosis, and decreases antimicrobial protein expression in human monocytes. J Leukoc Biol. 2009;86:1331-43 pubmed publisher
blocking or activating experiments; human; 20 ug/ml	Schlichting C, Schareck W, Weis M. Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors. J Cardiovasc Pharmacol. 2005;46:250-4 pubmed
immunohistochemistry; human	Garmy Susini B, Jin H, Zhu Y, Sung R, Hwang R, Varner J. Integrin alpha4beta1-VCAM-1-mediated adhesion between endothelial and mural cells is required for blood vessel maturation. J Clin Invest. 2005;115:1542-51 pubmed
immunohistochemistry; human	Burman A, Haworth O, Hardie D, Amft E, Siewert C, Jackson D, et al. A chemokine-dependent stromal induction mechanism for aberrant lymphocyte accumulation and compromised lymphatic return in rheumatoid arthritis. J Immunol. 2005;174:1693-700 pubmed
flow cytometry; human immunoprecipitation; human immunocytochemistry; human	Albelda S, Muller W, Buck C, Newman P. Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule. J Cell Biol. 1991;114:1059-68 pubmed
	Leveque E, Rouch A, Syrykh C, Mazieres J, Brouchet L, Valitutti S, et al. Phenotypic and Histological Distribution Analysis Identify Mast Cell Heterogeneity in Non-Small Cell Lung Cancer. Cancers (Basel). 2022;14: pubmed publisher
	Bond K, Chiba T, Wynne K, Vary C, Sims Lucas S, Coburn J, et al. The Extracellular Matrix Environment of Clear Cell Renal Cell Carcinoma Determines Cancer Associated Fibroblast Growth. Cancers (Basel). 2021;13: pubmed publisher
	Kir xe1 ly N, Thalwieser Z, Fon xf3 di M, Csortos C, Boratk xf3 A. Dephosphorylation of annexin A2 by protein phosphatase 1 regulates endothelial cell barrier. IUBMB Life. 2021;73:1257-1268 pubmed publisher
	Rahman I, Collado Sánchez A, Davies J, Rzeniewicz K, Abukscem S, Joachim J, et al. L-selectin regulates human neutrophil transendothelial migration. J Cell Sci. 2021;134: pubmed publisher
	Kook Y, Hwang S, Kim H, Rhee K, Lee K, Koh W. Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers. Sci Rep. 2020;10:20321 pubmed publisher
	Salman M, Marsh G, Kusters I, Delince M, Di Caprio G, Upadhyayula S, et al. Design and Validation of a Human Brain Endothelial Microvessel-on-a-Chip Open Microfluidic Model Enabling Advanced Optical Imaging. Front Bioeng Biotechnol. 2020;8:573775 pubmed publisher
	Lee D, Stewart G, Chambers M. Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus. Sci Rep. 2020;10:18495 pubmed publisher
	Laplagne C, Meddour S, Figarol S, Michelas M, Calvayrac O, Favre G, et al. Vγ9Vδ2 T Cells Activation Through Phosphoantigens Can Be Impaired by a RHOB Rerouting in Lung Cancer. Front Immunol. 2020;11:1396 pubmed publisher
	Lagrée A, Fasani F, Rouxel C, Pivet M, Pourcelot M, Fablet A, et al. Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections. Int J Mol Sci. 2020;21: pubmed publisher
	Han L, Choudhury S, Mich Basso J, Ammanamanchi N, Ganapathy B, Suresh S, et al. Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration. Dev Cell. 2020;53:42-59.e11 pubmed publisher
	Morgenroth A, Tinkir E, Vogg A, Sankaranarayanan R, Baazaoui F, Mottaghy F. Targeting of prostate-specific membrane antigen for radio-ligand therapy of triple-negative breast cancer. Breast Cancer Res. 2019;21:116 pubmed publisher
	Karki S, Farb M, Sharma V, Jash S, Zizza E, Hess D, et al. Fat-Specific Protein 27 Regulation of Vascular Function in Human Obesity. J Am Heart Assoc. 2019;8:e011431 pubmed publisher
	Luo W, Min J, Huang W, Wang X, Peng Y, Han S, et al. Vitexin reduces epilepsy after hypoxic ischemia in the neonatal brain via inhibition of NKCC1. J Neuroinflammation. 2018;15:186 pubmed publisher
	Li F, Huang J, Ji D, Meng Q, Wang C, Chen S, et al. Azithromycin effectively inhibits tumor angiogenesis by suppressing vascular endothelial growth factor receptor 2-mediated signaling pathways in lung cancer. Oncol Lett. 2017;14:89-96 pubmed publisher
	Totonchy J, Clepper L, Phillips K, McCarty O, Moses A. CXCR7 expression disrupts endothelial cell homeostasis and causes ligand-dependent invasion. Cell Adh Migr. 2014;8:165-76 pubmed
	Link A, Hardie D, Favre S, Britschgi M, Adams D, Sixt M, et al. Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans. Am J Pathol. 2011;178:1662-75 pubmed publisher
	Keller P, Lin A, Arendt L, Klebba I, Jones A, Rudnick J, et al. Mapping the cellular and molecular heterogeneity of normal and malignant breast tissues and cultured cell lines. Breast Cancer Res. 2010;12:R87 pubmed publisher
	Mamdouh Z, Kreitzer G, Muller W. Leukocyte transmigration requires kinesin-mediated microtubule-dependent membrane trafficking from the lateral border recycling compartment. J Exp Med. 2008;205:951-66 pubmed publisher
	Stucki A, Rivier A, Gikic M, Monai N, Schapira M, Spertini O. Endothelial cell activation by myeloblasts: molecular mechanisms of leukostasis and leukemic cell dissemination. Blood. 2001;97:2121-9 pubmed
	Chandrasekaran L, He C, Al Barazi H, Krutzsch H, Iruela Arispe M, Roberts D. Cell contact-dependent activation of alpha3beta1 integrin modulates endothelial cell responses to thrombospondin-1. Mol Biol Cell. 2000;11:2885-900 pubmed
	Kim S, Bell K, Mousa S, Varner J. Regulation of angiogenesis in vivo by ligation of integrin alpha5beta1 with the central cell-binding domain of fibronectin. Am J Pathol. 2000;156:1345-62 pubmed
	Yang S, Graham J, Kahn J, Schwartz E, Gerritsen M. Functional roles for PECAM-1 (CD31) and VE-cadherin (CD144) in tube assembly and lumen formation in three-dimensional collagen gels. Am J Pathol. 1999;155:887-95 pubmed
	Groger M, Matsumura T, Kohrgruber N, Maurer D, Wolff K, Petzelbauer P. A standardized, a computer-assisted in vitro assay for the assessment of neutrophil transmigration across endothelial monolayers. J Immunol Methods. 1999;222:101-9 pubmed
	Sauer A, Hartung T, Aigner J, Wendel A. Endotoxin-inducible granulocyte-mediated hepatocytotoxicity requires adhesion and serine protease release. J Leukoc Biol. 1996;60:633-43 pubmed
	Luscinskas F, Ding H, Tan P, Cumming D, Tedder T, Gerritsen M. L- and P-selectins, but not CD49d (VLA-4) integrins, mediate monocyte initial attachment to TNF-alpha-activated vascular endothelium under flow in vitro. J Immunol. 1996;157:326-35 pubmed
	Muller W, Weigl S, Deng X, Phillips D. PECAM-1 is required for transendothelial migration of leukocytes. J Exp Med. 1993;178:449-60 pubmed
	Muller W, Ratti C, McDonnell S, Cohn Z. A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions. J Exp Med. 1989;170:399-414 pubmed
	Newman P, Berndt M, Gorski J, White G, Lyman S, Paddock C, et al. PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science. 1990;247:1219-22 pubmed

product information

Product Type :

Antibody

Product Name :

CD31 Monoclonal Antibody (HEC7)

Catalog # :

MA3100

Quantity :

200 µg

Price :

US 420.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Human

Applications :

Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 1:50-1:500, Western Blot: 1:1,000

Species :

Bovine, Human

Clone :

HEC7

Isotype :

IgG2a

Storage :

-20°C

Description :

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Immunogen :

Human PECAM-1

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 1:50-1:500, Western Blot: 1:1,000

Aliases :

C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

more info or order :

Invitrogen product webpage