PARP1 Monoclonal Antibody (C-2-10) | Invitrogen MA3-950 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PARP1 Monoclonal Antibody (C-2-10)

catalog :

MA3-950

quantity :

50µL

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C-2-10

reactivity :

Chinese hamsters, human, mouse, rat, bovine

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human	Jelinic P, Levine D. New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair. Mol Cancer Ther. 2014;13:1645-54 pubmed publisher
western blot; human	Lin Y, Chen Y, Cohen S, Cheng T. Identification of TSG101 functional domains and p21 loci required for TSG101-mediated p21 gene regulation. PLoS ONE. 2013;8:e79674 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1500 western blot; human; 1:1500	Lonskaya I, Hebron M, Algarzae N, Desforges N, Moussa C. Decreased parkin solubility is associated with impairment of autophagy in the nigrostriatum of sporadic Parkinson's disease. Neuroscience. 2013;232:90-105 pubmed publisher
western blot; human	Wu X, Ellmann S, Rubin E, Gil M, Jin K, Han L, et al. ADP ribosylation by PARP-1 suppresses HOXB7 transcriptional activity. PLoS ONE. 2012;7:e40644 pubmed publisher
western blot; human	Yung T, Narita T, Komori T, Yamaguchi Y, Handa H. Cellular dynamics of the negative transcription elongation factor NELF. Exp Cell Res. 2009;315:1693-705 pubmed publisher
western blot; human	Komori T, Inukai N, Yamada T, Yamaguchi Y, Handa H. Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis. Genes Cells. 2009;14:343-54 pubmed publisher
western blot; mouse; 1:1000; fig 1A	Zhu C, Wang X, Huang Z, Qiu L, Xu F, Vahsen N, et al. Apoptosis-inducing factor is a major contributor to neuronal loss induced by neonatal cerebral hypoxia-ischemia. Cell Death Differ. 2007;14:775-84 pubmed
western blot; human; fig 2	Duensing A, Medeiros F, McConarty B, Joseph N, Panigrahy D, Singer S, et al. Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs). Oncogene. 2004;23:3999-4006 pubmed
	Mao H, Lockyer P, Townley Tilson W, Xie L, Pi X. LRP1 Regulates Retinal Angiogenesis by Inhibiting PARP-1 Activity and Endothelial Cell Proliferation. Arterioscler Thromb Vasc Biol. 2016;36:350-60 pubmed publisher

image

image 1 :

Immunofluorescent analysis of PARP in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PARP monoclonal antibody (Product # MA3-950) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PARP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of PARP in HT-29 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PARP monoclonal antibody (Product # MA3-950) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PARP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

PARP1 Monoclonal Antibody (C-2-10)

Catalog # :

MA3-950

Quantity :

50µL

Price :

US 390

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Applications :

ELISA: Assay dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500

Species :

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Clone :

C-2-10

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

Immunogen :

Purified calf thymus poly (ADP-ribose) polymerase.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500

Aliases :

5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; BOS_15645; C80510; DNA ADP-ribosyltransferase PARP1; I79_014850; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; pADPRT-1; Parp; PARP1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly ADP-ribose polymerase; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1

more info or order :

Invitrogen product webpage