Mu-Calpain Monoclonal Antibody (107-82) | Invitrogen MA3-942 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Mu-Calpain Monoclonal Antibody (107-82)

catalog :

MA3-942

quantity :

100µL

price :

US 345

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

107-82

reactivity :

rainbow trout, fission yeast, human, rat, bovine, pigs , domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; bovine; 1:1000; loading ...; tbl 1	Gagaoua M, Couvreur S, Le Bec G, Aminot G, Picard B. Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows. J Agric Food Chem. 2017;65:3569-3580 pubmed publisher
western blot; human; 1:500	Kumagai K, Ozaki Y, Nakanishi T, Inomata M, Furuno T, Nakanishi M, et al. Role of mu-calpain in human decidua for recurrent miscarriage. Am J Reprod Immunol. 2008;59:339-46 pubmed publisher
western blot; rainbow trout	Saito M, Li H, Thompson V, Kunisaki N, Goll D. Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss. Comp Biochem Physiol B Biochem Mol Biol. 2007;146:445-55 pubmed
western blot; bovine; 1:500	Cho Y, Choi H, Hwang I, Kim Y, Myung K. Effects of 25-hydroxyvitamin D3 and manipulated dietary cation-anion difference on the tenderness of beef from cull native Korean cows. J Anim Sci. 2006;84:1481-8 pubmed
western blot; fission yeast; 1:2,000	Robertson L, Morton J, Yamaguchi M, Bickerstaffe R, Shearer T, Azuma M. Calpain may contribute to hereditary cataract formation in sheep. Invest Ophthalmol Vis Sci. 2005;46:4634-40 pubmed
	Curry S, Burrough E, Schwartz K, Yoon K, Lonergan S, Gabler N. Porcine epidemic diarrhea virus reduces feed efficiency in nursery pigs. J Anim Sci. 2018;96:85-97 pubmed publisher
	Xu L, Deng X. Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone induces phosphorylation of mu- and m-calpain in association with increased secretion, cell migration, and invasion. J Biol Chem. 2004;279:53683-90 pubmed
	Sultan K, Dittrich B, Leisner E, Paul N, Pette D. Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle. Am J Physiol Cell Physiol. 2001;280:C239-47 pubmed
	Boehm M, Kendall T, Thompson V, Goll D. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J Anim Sci. 1998;76:2415-34 pubmed
	Cong J, Thompson V, Goll D. Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains. J Biol Chem. 1993;268:25740-7 pubmed
	Lane R, Allan D, Mellgren R. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp Cell Res. 1992;203:5-16 pubmed

image

image 1 :

Immunofluorescent analysis of M-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of M-Calpain in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 3 :

Immunofluorescent analysis of M-Calpain in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Mu-Calpain Monoclonal Antibody (107-82)

Catalog # :

MA3-942

Quantity :

100µL

Price :

US 345

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Bovine, Human, Porcine, Rabbit, Rat

Applications :

ELISA: Assay dependent, Flow Cytometry: Assay Dependent, Immunocytochemistry: 1:20, Immunofluorescence: 1:20, Immunohistochemistry (Frozen): 1:50, Western Blot: 1:500

Species :

Bovine, Human, Porcine, Rabbit, Rat

Clone :

107-82

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.

Immunogen :

Purified bovine skeletal muscle 80 kDa subunit of m-calpain.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay dependent, Flow Cytometry: Assay Dependent, Immunocytochemistry: 1:20, Immunofluorescence: 1:20, Immunohistochemistry (Frozen): 1:50, Western Blot: 1:500

Aliases :

BOS_24946; Calcium-activated neutral proteinase 1; calpain 1; calpain 1, (mu/I) large subunit; calpain 1, large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP; CANP 1; Canp1; CANPL1; Capa1; Capa-1; CAPN1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; Cls1; I79_024883; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; Micromolar-calpain; mu-calpain; mu-calpin; muCANP; muCL; PIG30; SPG76

more info or order :

Invitrogen product webpage