Mu-Calpain Monoclonal Antibody (9A4H8D3) | Invitrogen MA3-940 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Mu-Calpain Monoclonal Antibody (9A4H8D3)

catalog :

MA3-940

quantity :

100µg

price :

US 345

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9A4H8D3

reactivity :

fission yeast, rainbow trout, Chinese hamsters, red swamp crayfish, human, mouse, rat, bovine, pigs , domestic sheep, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 44

Published Application/Species/Sample/Dilution	Reference
western blot; domestic sheep; 1:1000; loading ...; fig 3	Du M, Li X, Li Z, Li M, Gao L, Zhang D. Phosphorylation inhibits the activity of ?-calpain at different incubation temperatures and Ca2+ concentrations in vitro. Food Chem. 2017;228:649-655 pubmed publisher
western blot; domestic sheep; 1:5000	Girard M, Dohme Meier F, Silacci P, Ampuero Kragten S, Kreuzer M, Bee G. Forage legumes rich in condensed tannins may increase n-3 fatty acid levels and sensory quality of lamb meat. J Sci Food Agric. 2016;96:1923-33 pubmed publisher
western blot; pigs	Foditsch E, Saenger A, Monticelli F. Skeletal muscle proteins: a new approach to delimitate the time since death. Int J Legal Med. 2016;130:433-40 pubmed publisher
western blot; pigs ; 1:10000	Wang J, Yan X, Liu R, Fu Q, Zhou G, Zhang W. Differences in calpain system, desmin degradation and water holding capacity between commercial Meishan and Durocâ€‰Ã—â€‰Landraceâ€‰Ã—â€‰Yorkshire crossbred pork. Anim Sci J. 2016;87:109-16 pubmed publisher
western blot; bovine; 1:5000	Cruzen S, Kim Y, Lonergan S, Grubbs J, Fritchen A, Huff Lonergan E. Effect of early postmortem enhancement of calcium lactate/phosphate on quality attributes of beef round muscles under different packaging systems. Meat Sci. 2015;101:63-72 pubmed publisher
western blot; pigs ; 1:10000	Yin Y, Zhang W, Zhou G, Guo B. Comparison of protein degradation, protein oxidation, and ?-calpain activation between pale, soft, and exudative and red, firm, and nonexudative pork during postmortem aging. J Anim Sci. 2014;92:3745-52 pubmed publisher
western blot; pigs ; 1:10000	Li Y, Liu R, Zhang W, Fu Q, Liu N, Zhou G. Effect of nitric oxide on ?-calpain activation, protein proteolysis, and protein oxidation of pork during post-mortem aging. J Agric Food Chem. 2014;62:5972-7 pubmed publisher
western blot; bovine; 1:5000	Lomiwes D, Hurst S, Dobbie P, Frost D, Hurst R, Young O, et al. The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins. Meat Sci. 2014;97:548-57 pubmed publisher
western blot; bovine; 1:10000	Pogge D, Lonergan S, Hansen S. Influence of supplementing vitamin C to yearling steers fed a high sulfur diet during the finishing period on meat color, tenderness and protein degradation, and fatty acid profile of the longissimus muscle. Meat Sci. 2014;97:419-27 pubmed publisher
immunocytochemistry; rat western blot; rat	Wang Y, Briz V, Chishti A, Bi X, Baudry M. Distinct roles for ?-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration. J Neurosci. 2013;33:18880-92 pubmed publisher
western blot; bovine; 1:10000	Pogge D, Lonergan S, Hansen S. Influence of supplemental vitamin C on postmortem protein degradation and fatty acid profiles of the longissimus thoracis of steers fed varying concentrations of dietary sulfur. Meat Sci. 2014;96:956-63 pubmed publisher
western blot; bovine; 1:10000	Cruzen S, Paulino P, Lonergan S, Huff Lonergan E. Postmortem proteolysis in three muscles from growing and mature beef cattle. Meat Sci. 2014;96:854-61 pubmed publisher
western blot; bovine; 1:5000	Lomiwes D, Farouk M, Wu G, Young O. The development of meat tenderness is likely to be compartmentalised by ultimate pH. Meat Sci. 2014;96:646-51 pubmed publisher
western blot; bovine; 1:5000	Lomiwes D, Farouk M, Frost D, Dobbie P, Young O. Small heat shock proteins and toughness in intermediate pHu beef. Meat Sci. 2013;95:472-9 pubmed publisher
western blot; domestic sheep; 1:10000	Kim Y, Luc G, Rosenvold K. Pre rigor processing, ageing and freezing on tenderness and colour stability of lamb loins. Meat Sci. 2013;95:412-8 pubmed publisher
western blot; pigs ; 1:10000	Cruzen S, Harris A, Hollinger K, Punt R, Grubbs J, Selsby J, et al. Evidence of decreased muscle protein turnover in gilts selected for low residual feed intake. J Anim Sci. 2013;91:4007-16 pubmed publisher
western blot; bovine; 1:10000	Magolski J, Berg E, Hall N, Anderson V, Keller W, Jeske T, et al. Evaluation of feedlot cattle working chute behavior relative to temperament, tenderness, and postmortem proteolysis. Meat Sci. 2013;95:92-7 pubmed publisher
western blot; bovine; 1:5000 immunoprecipitation; rat	Kim Y, Lonergan S, Grubbs J, Cruzen S, Fritchen A, Della Malva A, et al. Effect of low voltage electrical stimulation on protein and quality changes in bovine muscles during postmortem aging. Meat Sci. 2013;94:289-96 pubmed publisher
western blot; bovine	Anderson M, Lonergan S, Fedler C, Prusa K, Binning J, Huff Lonergan E. Profile of biochemical traits influencing tenderness of muscles from the beef round. Meat Sci. 2012;91:247-54 pubmed publisher
western blot; bovine; 1:10000	Kim Y, Stuart A, Nygaard G, Rosenvold K. High pre rigor temperature limits the ageing potential of beef that is not completely overcome by electrical stimulation and muscle restraining. Meat Sci. 2012;91:62-8 pubmed publisher
western blot; mouse	Bee G, Anderson A, Lonergan S, Huff Lonergan E. Rate and extent of pH decline affect proteolysis of cytoskeletal proteins and water-holding capacity in pork. Meat Sci. 2007;76:359-65 pubmed publisher
western blot; pigs ; 1:10000	Zhang W, Lonergan S, Gardner M, Huff Lonergan E. Contribution of postmortem changes of integrin, desmin and ?-calpain to variation in water holding capacity of pork. Meat Sci. 2006;74:578-85 pubmed publisher
western blot; mouse; 1:500	Nakajima T, Shearer T, Azuma M. Involvement of calpain 2 in ionomycin-induced cell death in cultured mouse lens epithelial cells. Curr Eye Res. 2011;36:930-6 pubmed publisher
immunohistochemistry; human immunohistochemistry; mouse	Vergo S, Craner M, Etzensperger R, Attfield K, Friese M, Newcombe J, et al. Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model. Brain. 2011;134:571-84 pubmed publisher
western blot; bovine; 1:10000	Kim Y, Lonergan S, Huff Lonergan E. Protein denaturing conditions in beef deep semimembranosus muscle results in limited ?-calpain activation and protein degradation. Meat Sci. 2010;86:883-7 pubmed publisher
western blot; bovine	Kim Y, Huff Lonergan E, Sebranek J, Lonergan S. High-oxygen modified atmosphere packaging system induces lipid and myoglobin oxidation and protein polymerization. Meat Sci. 2010;85:759-67 pubmed publisher
western blot; human	Cevik S, Hori Y, Kaplan O, Kida K, Toivenon T, Foley Fisher C, et al. Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans. J Cell Biol. 2010;188:953-69 pubmed publisher
western blot; red swamp crayfish; 1:4000	Chen G, Guttmann R, Xiong Y, Webster C, Romaire R. Protease activity in post-mortem red swamp crayfish (Procambarus clarkii) muscle stored in modified atmosphere packaging. J Agric Food Chem. 2008;56:8658-63 pubmed publisher
western blot; human; 1:2000	Kumagai K, Ozaki Y, Nakanishi T, Inomata M, Furuno T, Nakanishi M, et al. Role of mu-calpain in human decidua for recurrent miscarriage. Am J Reprod Immunol. 2008;59:339-46 pubmed publisher
western blot; bovine; 1:10,000	Camou J, Mares S, Marchello J, Vazquez R, Taylor M, Thompson V, et al. Isolation and characterization of mu-calpain, m-calpain, and calpastatin from postmortem muscle. I. Initial steps. J Anim Sci. 2007;85:3400-14 pubmed
western blot; bovine	Camou J, Marchello J, Thompson V, Mares S, Goll D. Effect of postmortem storage on activity of mu- and m-calpain in five bovine muscles. J Anim Sci. 2007;85:2670-81 pubmed
western blot; rainbow trout	Saito M, Li H, Thompson V, Kunisaki N, Goll D. Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss. Comp Biochem Physiol B Biochem Mol Biol. 2007;146:445-55 pubmed
western blot; bovine; 1:2000	Cho Y, Choi H, Hwang I, Kim Y, Myung K. Effects of 25-hydroxyvitamin D3 and manipulated dietary cation-anion difference on the tenderness of beef from cull native Korean cows. J Anim Sci. 2006;84:1481-8 pubmed
western blot; fission yeast; 1:2,000	Robertson L, Morton J, Yamaguchi M, Bickerstaffe R, Shearer T, Azuma M. Calpain may contribute to hereditary cataract formation in sheep. Invest Ophthalmol Vis Sci. 2005;46:4634-40 pubmed
western blot; pigs	Maddock K, Huff Lonergan E, Rowe L, Lonergan S. Effect of pH and ionic strength on mu- and m-calpain inhibition by calpastatin. J Anim Sci. 2005;83:1370-6 pubmed
	Blank C, Russell J, Lonergan S, Hansen S. Influence of feed efficiency classification and growing and finishing diet type on meat tenderness attributes of beef steers. J Anim Sci. 2017;95:2986-2992 pubmed publisher
	Rowe L, Maddock K, Lonergan S, Huff Lonergan E. Oxidative environments decrease tenderization of beef steaks through inactivation of mu-calpain. J Anim Sci. 2004;82:3254-66 pubmed
	Melody J, Lonergan S, Rowe L, Huiatt T, Mayes M, Huff Lonergan E. Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles. J Anim Sci. 2004;82:1195-205 pubmed
	Thompson V, Saldana S, Cong J, Luedke D, Goll D. The calpain system in human placenta. Life Sci. 2002;70:2493-508 pubmed
	Sultan K, Dittrich B, Leisner E, Paul N, Pette D. Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle. Am J Physiol Cell Physiol. 2001;280:C239-47 pubmed
	Reddy R, Lu J, Lee A. The endoplasmic reticulum chaperone glycoprotein GRP94 with Ca(2+)-binding and antiapoptotic properties is a novel proteolytic target of calpain during etoposide-induced apoptosis. J Biol Chem. 1999;274:28476-83 pubmed
	Boehm M, Kendall T, Thompson V, Goll D. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J Anim Sci. 1998;76:2415-34 pubmed
	Cong J, Thompson V, Goll D. Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains. J Biol Chem. 1993;268:25740-7 pubmed
	Lane R, Allan D, Mellgren R. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp Cell Res. 1992;203:5-16 pubmed

image

image 1 :

Immunofluorescent analysis of Mu-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mu-Calpain monoclonal antibody (Product # MA3-940) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mu-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Mu-Calpain Monoclonal Antibody (9A4H8D3)

Catalog # :

MA3-940

Quantity :

100µg

Price :

US 345

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Hamster, Human, Mouse, Porcine, Rabbit, Rat

Applications :

Immunocytochemistry: Assay dependent, Immunofluorescence: Assay dependent, Immunohistochemistry (Frozen): Assay dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 2 µg/mL

Species :

Bovine, Hamster, Human, Mouse, Porcine, Rabbit, Rat

Clone :

9A4H8D3

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.

Immunogen :

Purified bovine skeletal muscle 80 kDa mu-calpain subunit.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay dependent, Immunofluorescence: Assay dependent, Immunohistochemistry (Frozen): Assay dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 2 µg/mL

Aliases :

BOS_24946; Calcium-activated neutral proteinase 1; calpain 1; calpain 1, (mu/I) large subunit; calpain 1, large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP; CANP 1; Canp1; CANPL1; Capa1; Capa-1; CAPN1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; Cls1; I79_024883; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; Micromolar-calpain; mu-calpain; mu-calpin; muCANP; muCL; PIG30; SPG76

more info or order :

Invitrogen product webpage