Sarcalumenin Monoclonal Antibody (XIIC4) | Invitrogen MA3-932 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Sarcalumenin Monoclonal Antibody (XIIC4)

catalog :

MA3-932

quantity :

100µL

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

XIIC4

reactivity :

guinea pig, human, mouse, rat, dogs, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; domestic rabbit western blot; domestic rabbit; fig 3a	Wu A, Xu D, Yang N, Lin S, Chen P, Cala S, et al. Phospholamban is concentrated in the nuclear envelope of cardiomyocytes and involved in perinuclear/nuclear calcium handling. J Mol Cell Cardiol. 2016;100:1-8 pubmed publisher
western blot; mouse; 1:1000; fig 2	Mosca B, Eckhardt J, Bergamelli L, Treves S, Bongianino R, De Negri M, et al. Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles. J Biol Chem. 2016;291:14555-65 pubmed publisher
western blot; human; fig 1	Sekulic Jablanovic M, Ullrich N, Goldblum D, Palmowski Wolfe A, Zorzato F, Treves S. Functional characterization of orbicularis oculi and extraocular muscles. J Gen Physiol. 2016;147:395-406 pubmed publisher
western blot; human	Sekulic Jablanovic M, Palmowski Wolfe A, Zorzato F, Treves S. Characterization of excitation-contraction coupling components in human extraocular muscles. Biochem J. 2015;466:29-36 pubmed publisher
immunohistochemistry; human; 1:100	Di Blasi C, Blasevich F, Bellafiore E, Mottarelli E, Gibertini S, Zanotti S, et al. Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy. Neuromuscul Disord. 2010;20:326-9 pubmed publisher
western blot; mouse	Doran P, Dowling P, Donoghue P, Buffini M, Ohlendieck K. Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle. Biochim Biophys Acta. 2006;1764:773-85 pubmed
immunohistochemistry; human	Chevessier F, Bauché Godard S, Leroy J, Koenig J, Paturneau Jouas M, Eymard B, et al. The origin of tubular aggregates in human myopathies. J Pathol. 2005;207:313-23 pubmed
western blot; domestic rabbit	Sacchetto R, Bovo E, Donella Deana A, Damiani E. Glycogen- and PP1c-targeting subunit GM is phosphorylated at Ser48 by sarcoplasmic reticulum-bound Ca2+-calmodulin protein kinase in rabbit fast twitch skeletal muscle. J Biol Chem. 2005;280:7147-55 pubmed
	Doran P, Dowling P, Lohan J, McDonnell K, Poetsch S, Ohlendieck K. Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle. Eur J Biochem. 2004;271:3943-52 pubmed
	Dowling P, Doran P, Ohlendieck K. Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophy. Biochem J. 2004;379:479-88 pubmed
	Glover L, Heffron J, Ohlendieck K. Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle. J Appl Physiol (1985). 2004;96:11-8 pubmed
	Froemming G, Murray B, Harmon S, Pette D, Ohlendieck K. Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers. Biochim Biophys Acta. 2000;1466:151-68 pubmed
	Ohlendieck K, Frömming G, Murray B, Maguire P, Leisner E, Traub I, et al. Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle. Pflugers Arch. 1999;438:700-8 pubmed
	Kutchai H, Campbell K. Calcium transport by sarcoplasmic reticulum of skeletal muscle is inhibited by antibodies against the 53-kilodalton glycoprotein of the sarcoplasmic reticulum membrane. Biochemistry. 1989;28:4830-9 pubmed
	Leberer E, Timms B, Campbell K, MacLennan D. Purification, calcium binding properties, and ultrastructural localization of the 53,000- and 160,000 (sarcalumenin)-dalton glycoproteins of the sarcoplasmic reticulum. J Biol Chem. 1990;265:10118-24 pubmed
	Movsesian M, Leveille C, Krall J, Colyer J, Wang J, Campbell K. Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles. J Mol Cell Cardiol. 1990;22:1477-85 pubmed
	Ohlendieck K, Briggs F, Lee K, Wechsler A, Campbell K. Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle. Eur J Biochem. 1991;202:739-47 pubmed

product information

Product Type :

Antibody

Product Name :

Sarcalumenin Monoclonal Antibody (XIIC4)

Catalog # :

MA3-932

Quantity :

100µL

Price :

US 390

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Guinea Pig, Mouse, Rabbit, Rat

Applications :

Immunofluorescence: 1/500, Western Blot: 1-3 µg/mL

Species :

Canine, Guinea Pig, Mouse, Rabbit, Rat

Clone :

XIIC4

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner. The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.

Immunogen :

Purified rabbit sarcoplasmic reticulum membrane vesicles.

Format :

Liquid

Applications w/Dilutions :

Immunofluorescence: 1/500, Western Blot: 1-3 µg/mL

Aliases :

9830004M20Rik; AW108387; Sar; Sarcalumenin; sarcolumenin; sarcoplasmic reticulum glycoprotein; SRL

more info or order :

Invitrogen product webpage