Triadin Monoclonal Antibody (IIG12) | Invitrogen MA3-931 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Triadin Monoclonal Antibody (IIG12)

catalog :

MA3-931

quantity :

100 µL

price :

US 428

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IIG12

reactivity :

mouse, domestic rabbit

application :

western blot, immunohistochemistry

citations: 14

Reference
Aracena P, Sanchez G, Donoso P, Hamilton S, Hidalgo C. S-glutathionylation decreases Mg2+ inhibition and S-nitrosylation enhances Ca2+ activation of RyR1 channels. J Biol Chem. 2003;278:42927-35 pubmed
Mulvey C, Ohlendieck K. Use of continuous-elution gel electrophoresis as a preparative tool for blot overlay analysis. Anal Biochem. 2003;319:122-30 pubmed
Glover L, Quinn S, Ryan M, Pette D, Ohlendieck K. Supramolecular calsequestrin complex. Eur J Biochem. 2002;269:4607-16 pubmed
Glover L, Culligan K, Cala S, Mulvey C, Ohlendieck K. Calsequestrin binds to monomeric and complexed forms of key calcium-handling proteins in native sarcoplasmic reticulum membranes from rabbit skeletal muscle. Biochim Biophys Acta. 2001;1515:120-32 pubmed
Harmon S, Froemming G, Leisner E, Pette D, Ohlendieck K. Low-frequency stimulation of fast muscle affects the abundance of Ca(2+)-ATPase but not its oligomeric status. J Appl Physiol (1985). 2001;90:371-9 pubmed
Zorzato F, Anderson A, Ohlendieck K, Froemming G, Guerrini R, Treves S. Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic-reticulum junctional-face membrane. Biochem J. 2000;351 Pt 2:537-43 pubmed
Ohlendieck K, Frömming G, Murray B, Maguire P, Leisner E, Traub I, et al. Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle. Pflugers Arch. 1999;438:700-8 pubmed
Moore R, Nguyen H, Galceran J, Pessah I, Allen P. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function. J Cell Biol. 1998;140:843-51 pubmed
Guo W, Jorgensen A, Jones L, Campbell K. Biochemical characterization and molecular cloning of cardiac triadin. J Biol Chem. 1996;271:458-65 pubmed
Knudson C, Stang K, Jorgensen A, Campbell K. Biochemical characterization of ultrastructural localization of a major junctional sarcoplasmic reticulum glycoprotein (triadin). J Biol Chem. 1993;268:12637-45 pubmed
Guo W, Jorgensen A, Campbell K. Characterization and ultrastructural localization of a novel 90-kDa protein unique to skeletal muscle junctional sarcoplasmic reticulum. J Biol Chem. 1994;269:28359-65 pubmed
Liu G, Pessah I. Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem. 1994;269:33028-34 pubmed
Imagawa T, Leung A, Campbell K. Phosphorylation of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel by an intrinsic protein kinase in isolated triads from rabbit skeletal muscle. J Biol Chem. 1987;262:8333-9 pubmed
Campbell K, Knudson C, Imagawa T, Leung A, Sutko J, Kahl S, et al. Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel. J Biol Chem. 1987;262:6460-3 pubmed

product information

Product Type :

Antibody

Product Name :

Triadin Monoclonal Antibody (IIG12)

Catalog # :

MA3-931

Quantity :

100 µL

Pricing :

US 428

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Mouse, Rabbit

Applications :

Immunohistochemistry: 1:500, Western Blot: 1:1,000

Species :

Mouse, Rabbit

Clone :

IIG12

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR’s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR’s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR’s and RyR’s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Immunogen :

Rabbit skeletal muscle triads.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: 1:500, Western Blot: 1:1,000

Aliases :

2310045H21Rik; cardiac triadin; cardiac triadin isoform 1; cardiac triadin isoform 2; cardiac triadin isoform 3; CPVT5; EG432451; junctional sarcoplasmic reticulum glycoprotein; TDN; Trdn; Triadin; triadin 1; triadin 32 kDa (TRISK 32); triadin 49 kDa (TRISK 49); triadin 95; TRISK