ATP1B1 Monoclonal Antibody (M17-P5-F11) | Invitrogen MA3-930 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ATP1B1 Monoclonal Antibody (M17-P5-F11)

catalog :

MA3-930

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M17-P5-F11

reactivity :

human, mouse, dogs, pigs , domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 52

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; loading ...; fig 2d	Wyckelsma V, Levinger I, Murphy R, Petersen A, Perry B, Hedges C, et al. Intense interval training in healthy older adults increases skeletal muscle [3H]ouabain-binding site content and elevates Na+,K+-ATPase ?2 isoform abundance in Type II fibers. Physiol Rep. 2017;5: pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig s9b	Afshari N, Igo R, Morris N, Stambolian D, Sharma S, Pulagam V, et al. Genome-wide association study identifies three novel loci in Fuchs endothelial corneal dystrophy. Nat Commun. 2017;8:14898 pubmed publisher
western blot; human; loading ...; fig 4	Skovgaard C, Almquist N, Bangsbo J. Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners. J Appl Physiol (1985). 2017;122:48-59 pubmed publisher
western blot; human; 1:1500; loading ...; fig 5d	Perry B, Wyckelsma V, Murphy R, Steward C, Anderson M, Levinger I, et al. Dissociation between short-term unloading and resistance training effects on skeletal muscle Na+,K+-ATPase, muscle function, and fatigue in humans. J Appl Physiol (1985). 2016;121:1074-1086 pubmed publisher
western blot; human; 1:1000; fig 2	Vorup J, Tybirk J, Gunnarsson T, Ravnholt T, Dalsgaard S, Bangsbo J. Effect of speed endurance and strength training on performance, running economy and muscular adaptations in endurance-trained runners. Eur J Appl Physiol. 2016;116:1331-41 pubmed publisher
western blot; human; 1:1000; fig 3	Tokhtaeva E, Sun H, Deiss Yehiely N, Wen Y, Soni P, Gabrielli N, et al. The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase β1 subunits. J Cell Sci. 2016;129:2394-406 pubmed publisher
western blot; human; 1:500; fig 1	Wyckelsma V, McKenna M, Levinger I, Petersen A, Lamboley C, Murphy R. Cell specific differences in the protein abundances of GAPDH and Na(+),K(+)-ATPase in skeletal muscle from aged individuals. Exp Gerontol. 2016;75:8-15 pubmed publisher
western blot; human; fig 1	Mohr M, Thomassen M, Girard O, Racinais S, Nybo L. Muscle variables of importance for physiological performance in competitive football. Eur J Appl Physiol. 2016;116:251-62 pubmed publisher
western blot; human; fig 3c	Hostrup M, Kalsen A, Onslev J, Jessen S, Haase C, Habib S, et al. Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic Î²2-adrenergic stimulation in men. J Appl Physiol (1985). 2015;119:475-86 pubmed publisher
western blot; human	Perry B, Levinger P, Morris H, Petersen A, Garnham A, Levinger I, et al. The effects of knee injury on skeletal muscle function, Na+, K+-ATPase content, and isoform abundance. Physiol Rep. 2015;3: pubmed publisher
western blot; human	Skovgaard C, Christensen P, Larsen S, Andersen T, Thomassen M, Bangsbo J. Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners. J Appl Physiol (1985). 2014;117:1097-109 pubmed publisher
western blot; human; 1:5000; fig 3	Prasad M, Bhalla K, Pan Z, O Connell J, Weder A, Chakravarti A, et al. A polymorphic 3'UTR element in ATP1B1 regulates alternative polyadenylation and is associated with blood pressure. PLoS ONE. 2013;8:e76290 pubmed publisher
western blot; human	Perry B, Levinger P, Serpiello F, Caldow M, Cameron Smith D, Bartlett J, et al. The effects of osteoarthritis and age on skeletal muscle strength, Na+-K+-ATPase content, gene and isoform expression. J Appl Physiol (1985). 2013;115:1443-9 pubmed publisher
western blot; human; 1:1000	Gunnarsson T, Christensen P, Thomassen M, Nielsen L, Bangsbo J. Effect of intensified training on muscle ion kinetics, fatigue development, and repeated short-term performance in endurance-trained cyclists. Am J Physiol Regul Integr Comp Physiol. 2013;305:R811-21 pubmed publisher
western blot; human	McKenna M, Perry B, Serpiello F, Caldow M, Levinger P, Cameron Smith D, et al. Unchanged [3H]ouabain binding site content but reduced Na+-K+ pump α2-protein abundance in skeletal muscle in older adults. J Appl Physiol (1985). 2012;113:1505-11 pubmed publisher
immunocytochemistry; dogs	Tokhtaeva E, Clifford R, Kaplan J, Sachs G, Vagin O. Subunit isoform selectivity in assembly of Na,K-ATPase ?-? heterodimers. J Biol Chem. 2012;287:26115-25 pubmed publisher
western blot; human; 1:1000	Gunnarsson T, Bangsbo J. The 10-20-30 training concept improves performance and health profile in moderately trained runners. J Appl Physiol (1985). 2012;113:16-24 pubmed publisher
western blot; human	Nordsborg N, Siebenmann C, Jacobs R, Rasmussen P, Diaz V, Robach P, et al. Four weeks of normobaric "live high-train low" do not alter muscular or systemic capacity for maintaining pH and K? homeostasis during intense exercise. J Appl Physiol (1985). 2012;112:2027-36 pubmed publisher
western blot; dogs	Tokhtaeva E, Sachs G, Sun H, Dada L, Sznajder J, Vagin O. Identification of the amino acid region involved in the intercellular interaction between the ?1 subunits of Na+/K+ -ATPase. J Cell Sci. 2012;125:1605-16 pubmed publisher
western blot; human	Boon H, Kostovski E, Pirkmajer S, Song M, Lubarski I, Iversen P, et al. Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans. Am J Physiol Endocrinol Metab. 2012;302:E864-71 pubmed publisher
western blot; human	Petersen A, Leikis M, McMahon L, Kent A, Murphy K, Gong X, et al. Impaired exercise performance and muscle Na(+),K(+)-pump activity in renal transplantation and haemodialysis patients. Nephrol Dial Transplant. 2012;27:2036-43 pubmed publisher
western blot; human	Benziane B, Widegren U, Pirkmajer S, Henriksson J, Stepto N, Chibalin A. Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle. Am J Physiol Endocrinol Metab. 2011;301:E456-66 pubmed publisher
western blot; dogs	Tokhtaeva E, Sachs G, Souda P, Bassilian S, Whitelegge J, Shoshani L, et al. Epithelial junctions depend on intercellular trans-interactions between the Na,K-ATPase ?? subunits. J Biol Chem. 2011;286:25801-12 pubmed publisher
western blot; human	Iaia F, Perez Gomez J, Thomassen M, Nordsborg N, Hellsten Y, Bangsbo J. Relationship between performance at different exercise intensities and skeletal muscle characteristics. J Appl Physiol (1985). 2011;110:1555-63 pubmed publisher
western blot; human	Tokhtaeva E, Sachs G, Vagin O. Diverse pathways for maturation of the Na,K-ATPase ?1 and ?2 subunits in the endoplasmic reticulum of Madin-Darby canine kidney cells. J Biol Chem. 2010;285:39289-302 pubmed publisher
western blot; human	Thomassen M, Christensen P, Gunnarsson T, Nybo L, Bangsbo J. Effect of 2-wk intensified training and inactivity on muscle Na+-K+ pump expression, phospholemman (FXYD1) phosphorylation, and performance in soccer players. J Appl Physiol (1985). 2010;108:898-905 pubmed publisher
western blot; human; 1:1,000	Bangsbo J, Gunnarsson T, Wendell J, Nybo L, Thomassen M. Reduced volume and increased training intensity elevate muscle Na+-K+ pump alpha2-subunit expression as well as short- and long-term work capacity in humans. J Appl Physiol (1985). 2009;107:1771-80 pubmed publisher
western blot; domestic sheep; 1:200,000	Clifford R, Kaplan J. Regulation of Na,K-ATPase subunit abundance by translational repression. J Biol Chem. 2009;284:22905-15 pubmed publisher
western blot; human	Lecuona E, Sun H, Vohwinkel C, Ciechanover A, Sznajder J. Ubiquitination participates in the lysosomal degradation of Na,K-ATPase in steady-state conditions. Am J Respir Cell Mol Biol. 2009;41:671-9 pubmed publisher
western blot; human; 1:1,000	Iaia F, Thomassen M, Kolding H, Gunnarsson T, Wendell J, Rostgaard T, et al. Reduced volume but increased training intensity elevates muscle Na+-K+ pump alpha1-subunit and NHE1 expression as well as short-term work capacity in humans. Am J Physiol Regul Integr Comp Physiol. 2008;294:R966-74 pubmed
immunocytochemistry; human immunohistochemistry; mouse	Zimnicka A, Maryon E, Kaplan J. Human copper transporter hCTR1 mediates basolateral uptake of copper into enterocytes: implications for copper homeostasis. J Biol Chem. 2007;282:26471-80 pubmed
western blot; human	Aughey R, Murphy K, Clark S, Garnham A, Snow R, Cameron Smith D, et al. Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes. J Appl Physiol (1985). 2007;103:39-47 pubmed
western blot; dogs; 1:400,000	Laughery M, Clifford R, Chi Y, Kaplan J. Selective basolateral localization of overexpressed Na-K-ATPase beta1- and beta2- subunits is disrupted by butryate treatment of MDCK cells. Am J Physiol Renal Physiol. 2007;292:F1718-25 pubmed
western blot; human; 1:5,000	Murphy K, Petersen A, Goodman C, Gong X, Leppik J, Garnham A, et al. Prolonged submaximal exercise induces isoform-specific Na+-K+-ATPase mRNA and protein responses in human skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2006;290:R414-24 pubmed
	Christiansen D, Eibye K, Rasmussen V, Voldbye H, Thomassen M, Nyberg M, et al. Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of anti-oxidant infusion in humans. J Physiol. 2019;597:2421-2444 pubmed publisher
	Fransson D, Nielsen T, Olsson K, Christensson T, Bradley P, Fatouros I, et al. Skeletal muscle and performance adaptations to high-intensity training in elite male soccer players: speed endurance runs versus small-sided game training. Eur J Appl Physiol. 2018;118:111-121 pubmed publisher
	Skovgaard C, Almquist N, Kvorning T, Christensen P, Bangsbo J. Effect of tapering after a period of high-volume sprint interval training on running performance and muscular adaptations in moderately trained runners. J Appl Physiol (1985). 2018;124:259-267 pubmed publisher
	Mohr M, Nielsen T, Weihe P, Thomsen J, Aquino G, Krustrup P, et al. Muscle ion transporters and antioxidative proteins have different adaptive potential in arm than in leg skeletal muscle with exercise training. Physiol Rep. 2017;5: pubmed publisher
	Liu W, Schrott Fischer A, Glueckert R, Benav H, Rask Andersen H. The Human "Cochlear Battery" - Claudin-11 Barrier and Ion Transport Proteins in the Lateral Wall of the Cochlea. Front Mol Neurosci. 2017;10:239 pubmed publisher
	Wyckelsma V, McKenna M, Serpiello F, Lamboley C, Aughey R, Stepto N, et al. Single-fiber expression and fiber-specific adaptability to short-term intense exercise training of Na+-K+-ATPase Î±- and Î²-isoforms in human skeletal muscle. J Appl Physiol (1985). 2015;118:699-706 pubmed publisher
	Thomassen M, Murphy R, Bangsbo J. Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans. J Physiol. 2013;591:1523-33 pubmed publisher
	Suñé G, Sarró E, Puigmulé M, López Hellín J, Zufferey M, Pertel T, et al. Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney. PLoS ONE. 2010;5:e13930 pubmed publisher
	Clifford R, Kaplan J. beta-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells. Am J Physiol Renal Physiol. 2008;295:F1314-23 pubmed publisher
	Nordsborg N, Ovesen J, Thomassen M, Zangenberg M, Jøns C, Iaia F, et al. Effect of dexamethasone on skeletal muscle Na+,K+ pump subunit specific expression and K+ homeostasis during exercise in humans. J Physiol. 2008;586:1447-59 pubmed publisher
	Vagin O, Tokhtaeva E, Sachs G. The role of the beta1 subunit of the Na,K-ATPase and its glycosylation in cell-cell adhesion. J Biol Chem. 2006;281:39573-87 pubmed
	Laughery M, Todd M, Kaplan J. Oligomerization of the Na,K-ATPase in cell membranes. J Biol Chem. 2004;279:36339-48 pubmed
	Murphy K, Snow R, Petersen A, Murphy R, Mollica J, Lee J, et al. Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle. J Physiol. 2004;556:507-19 pubmed
	Greig E, Mathialahan T, Boot Handford R, Sandle G. Molecular and functional studies of electrogenic Na(+) transport in the distal colon and rectum of young and elderly subjects. Gut. 2003;52:1607-15 pubmed
	Laughery M, Todd M, Kaplan J. Mutational analysis of alpha-beta subunit interactions in the delivery of Na,K-ATPase heterodimers to the plasma membrane. J Biol Chem. 2003;278:34794-803 pubmed
	Hoffman J, Wickrema A, Potapova O, Milanick M, Yingst D. Na pump isoforms in human erythroid progenitor cells and mature erythrocytes. Proc Natl Acad Sci U S A. 2002;99:14572-7 pubmed
	Bortner C, Gómez Angelats M, Cidlowski J. Plasma membrane depolarization without repolarization is an early molecular event in anti-Fas-induced apoptosis. J Biol Chem. 2001;276:4304-14 pubmed
	Sun Y, Ball W. Determination of Na(+)-K(+)-ATPase alpha- and beta-isoforms and kinetic properties in mammalian liver. Am J Physiol. 1992;262:C1491-9 pubmed

product information

Product Type :

Antibody

Product Name :

ATP1B1 Monoclonal Antibody (M17-P5-F11)

Catalog # :

MA3-930

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Ovine, Porcine

Applications :

Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:10,000

Species :

Canine, Human, Mouse, Ovine, Porcine

Clone :

M17-P5-F11

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.

Immunogen :

Purified lamb kidney beta sodium/potassium ATPase.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:10,000

Aliases :

adenosinetriphosphatase; ATP1B; ATP1B1; Atp4b; ATPase beta subunit; ATPase Na+/K+ transporting subunit beta 1; ATPase, Na+/K+ transporting, beta 1 polypeptide; Atpb; Atpb-1; ATPBS; Beta 1-subunit of Na(+),K(+)-ATPase; hypothetical protein LOC519758; MGC1798; Na, K-ATPase beta-1 polypeptide; Na,K-ATPase beta 1 subunit; Na+, K+-ATPase; Na+, K+-ATPase beta-subunit; Na+,K+-ATPase beta 1 subunit; Na+/K+ ATPase Beta; NKbeta1; OTTHUMP00000032537; OTTHUMP00000032538; P-type ATPase; Sodium Potassium ATPase; sodium pump subunit beta-1; sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; sodium/potassium-transporting ATPase subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); unnamed protein product

more info or order :

Invitrogen product webpage