ATP1A1 Monoclonal Antibody (M8-P1-A3) | Invitrogen MA3-929 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ATP1A1 Monoclonal Antibody (M8-P1-A3)

catalog :

MA3-929

quantity :

100µg

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M8-P1-A3

reactivity :

fission yeast, African green monkey, human, mouse, rat, dogs, pigs , domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 48

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 2d	Lobato Álvarez J, Roldán M, López Murillo T, González Ramírez R, Bonilla Delgado J, Shoshani L. The Apical Localization of Na+, K+-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the ?2 Subunit. Front Physiol. 2016;7:450 pubmed
western blot; human; 1:100; loading ...; fig 7i	Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed
immunohistochemistry; dogs; loading ...; fig 3b	Ponce A, Larre I, Castillo A, Flores Maldonado C, Verdejo Torres O, Contreras R, et al. Ouabain Modulates the Distribution of Connexin 43 in Epithelial Cells. Cell Physiol Biochem. 2016;39:1329-38 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3	Al Kafaji G, Sabry M, Bakhiet M. Increased expression of mitochondrial DNA-encoded genes in human renal mesangial cells in response to high glucose-induced reactive oxygen species. Mol Med Rep. 2016;13:1774-80 pubmed publisher
immunohistochemistry; human	Lee J, Shahidullah M, Hotchkiss A, Coca Prados M, Delamere N, Pelis R. A renal-like organic anion transport system in the ciliary epithelium of the bovine and human eye. Mol Pharmacol. 2015;87:697-705 pubmed publisher
immunoprecipitation; mouse western blot; mouse; 1:2000	Wang F, Cai B, Li K, Hu X, Lu Y, Wang Q, et al. FXYD2, a Î³ subunit of Naâº, Kâº-ATPase, maintains persistent mechanical allodynia induced by inflammation. Cell Res. 2015;25:318-34 pubmed publisher
immunocytochemistry; African green monkey	García Hernández V, Flores Maldonado C, Rincon Heredia R, Verdejo Torres O, Bonilla Delgado J, Meneses Morales I, et al. EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells. J Cell Physiol. 2015;230:105-15 pubmed publisher
western blot; human; 1:200	Bednarczyk P, Koziel A, Jarmuszkiewicz W, Szewczyk A. Large-conductance Ca²?-activated potassium channel in mitochondria of endothelial EA.hy926 cells. Am J Physiol Heart Circ Physiol. 2013;304:H1415-27 pubmed publisher
western blot; mouse	Pinder J, Taylor Harris P, Bennett P, Carter E, Hayes N, King M, et al. Isoforms of protein 4.1 are differentially distributed in heart muscle cells: relation of 4.1R and 4.1G to components of the Ca2+ homeostasis system. Exp Cell Res. 2012;318:1467-79 pubmed publisher
immunocytochemistry; dogs; loading ...; fig 3k	Larre I, Castillo A, Flores Maldonado C, Contreras R, Galván I, Muñoz Estrada J, et al. Ouabain modulates ciliogenesis in epithelial cells. Proc Natl Acad Sci U S A. 2011;108:20591-6 pubmed publisher
western blot; African green monkey; 10 nM	Paroder Belenitsky M, Maestas M, Dohan O, Nicola J, Reyna Neyra A, Follenzi A, et al. Mechanism of anion selectivity and stoichiometry of the Na+/I- symporter (NIS). Proc Natl Acad Sci U S A. 2011;108:17933-8 pubmed publisher
immunocytochemistry; dogs; 1:500	Lyons P, Fricker L. Carboxypeptidase O is a glycosylphosphatidylinositol-anchored intestinal peptidase with acidic amino acid specificity. J Biol Chem. 2011;286:39023-32 pubmed publisher
western blot; human; 1:1000	Chen J, Weber M, Um S, Walsh C, Tang Y, McDonald T. A dual mechanism for I(Ks) current reduction by the pathogenic mutation KCNQ1-S277L. Pacing Clin Electrophysiol. 2011;34:1652-64 pubmed publisher
immunocytochemistry; human	Gibbons T, Storey S, Williams C, McIntosh A, Mitchel D, Parr R, et al. Rotavirus NSP4: Cell type-dependent transport kinetics to the exofacial plasma membrane and release from intact infected cells. Virol J. 2011;8:278 pubmed publisher
western blot; fission yeast; 1:10000	Keller Wood M, Wood C, McCartney J, Jesse N, Perrone D. A role for mineralocorticoid receptors in the physiology of the ovine fetus: effects on ACTH and lung liquid composition. Pediatr Res. 2011;69:491-6 pubmed publisher
western blot; rat	Benipal B, Lash L. Influence of renal compensatory hypertrophy on mitochondrial energetics and redox status. Biochem Pharmacol. 2011;81:295-303 pubmed publisher
western blot; human	Perrotta C, Bizzozero L, Cazzato D, Morlacchi S, Assi E, Simbari F, et al. Syntaxin 4 is required for acid sphingomyelinase activity and apoptotic function. J Biol Chem. 2010;285:40240-51 pubmed publisher
immunocytochemistry; rat	Rao A, Simmons D, Sorkin A. Differential subcellular distribution of endosomal compartments and the dopamine transporter in dopaminergic neurons. Mol Cell Neurosci. 2011;46:148-58 pubmed publisher
western blot; mouse; 1:1000	Hyun D, Mughal M, Yang H, Lee J, Ko E, Hunt N, et al. The plasma membrane redox system is impaired by amyloid ?-peptide and in the hippocampus and cerebral cortex of 3xTgAD mice. Exp Neurol. 2010;225:423-9 pubmed publisher
western blot; mouse	Alekseev A, Reyes S, Yamada S, Hodgson Zingman D, Sattiraju S, Zhu Z, et al. Sarcolemmal ATP-sensitive K(+) channels control energy expenditure determining body weight. Cell Metab. 2010;11:58-69 pubmed publisher
western blot; domestic sheep; 1:200,000	Clifford R, Kaplan J. Regulation of Na,K-ATPase subunit abundance by translational repression. J Biol Chem. 2009;284:22905-15 pubmed publisher
western blot; domestic sheep; 1:10000	Keller Wood M, Von Reitzenstein M, McCartney J. Is the fetal lung a mineralocorticoid receptor target organ? Induction of cortisol-regulated genes in the ovine fetal lung, kidney and small intestine. Neonatology. 2009;95:47-60 pubmed publisher
western blot; dogs; 1:400,000	Laughery M, Clifford R, Chi Y, Kaplan J. Selective basolateral localization of overexpressed Na-K-ATPase beta1- and beta2- subunits is disrupted by butryate treatment of MDCK cells. Am J Physiol Renal Physiol. 2007;292:F1718-25 pubmed
western blot; human	Xiong L, Zhang J, He R, Hamilton S. A Ca2+-binding domain in RyR1 that interacts with the calmodulin binding site and modulates channel activity. Biophys J. 2006;90:173-82 pubmed
western blot; human; 1:500	Fernandez Ayala D, Brea Calvo G, Lopez Lluch G, Navas P. Coenzyme Q distribution in HL-60 human cells depends on the endomembrane system. Biochim Biophys Acta. 2005;1713:129-37 pubmed
immunohistochemistry; domestic sheep; 1:200	Keller Wood M, Wood C, Hua Y, Zhang D. Mineralocorticoid receptor expression in late-gestation ovine fetal lung. J Soc Gynecol Investig. 2005;12:84-91 pubmed
	Burat B, Gonzalez J, Sauvage F, Aouad H, Arnion H, Pinault E, et al. Sum of peak intensities outperforms peak area integration in iTRAQ protein expression measurement by LC-MS/MS using a TripleTOF 5600+ platform. Biosci Rep. 2019;: pubmed publisher
	Paul D, Soignier R, Minor L, Tau H, Songu Mize E, Gould H. Regulation and pharmacological blockade of sodium-potassium ATPase: a novel pathway to neuropathy. J Neurol Sci. 2014;340:139-43 pubmed publisher
	Clifford R, Kaplan J. beta-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells. Am J Physiol Renal Physiol. 2008;295:F1314-23 pubmed publisher
	Lunde P, Sejersted O, Thorud H, Tønnessen T, Henriksen U, Christensen G, et al. Effects of congestive heart failure on Ca2+ handling in skeletal muscle during fatigue. Circ Res. 2006;98:1514-9 pubmed
	Laughery M, Todd M, Kaplan J. Oligomerization of the Na,K-ATPase in cell membranes. J Biol Chem. 2004;279:36339-48 pubmed
	Hale M, Marvaud J, Popoff M, Stiles B. Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin. Infect Immun. 2004;72:2186-93 pubmed
	Quaedackers J, Roelfsema V, Hunter C, Heineman E, Gunn A, Bennet L. Polyuria and impaired renal blood flow after asphyxia in preterm fetal sheep. Am J Physiol Regul Integr Comp Physiol. 2004;286:R576-83 pubmed
	Keller R, Arnold T, Fliesler S. Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome. J Lipid Res. 2004;45:347-55 pubmed
	Kip S, Strehler E. Vitamin D3 upregulates plasma membrane Ca2+-ATPase expression and potentiates apico-basal Ca2+ flux in MDCK cells. Am J Physiol Renal Physiol. 2004;286:F363-9 pubmed
	Laughery M, Todd M, Kaplan J. Mutational analysis of alpha-beta subunit interactions in the delivery of Na,K-ATPase heterodimers to the plasma membrane. J Biol Chem. 2003;278:34794-803 pubmed
	Johansson C, Lunde P, Gothe S, Lannergren J, Westerblad H. Isometric force and endurance in skeletal muscle of mice devoid of all known thyroid hormone receptors. J Physiol. 2003;547:789-96 pubmed
	Scheiner Bobis G, Hübschle T, Diener M. Action of palytoxin on apical H+/K+-ATPase in rat colon. Eur J Biochem. 2002;269:3905-11 pubmed
	Tsivkovskii R, Eisses J, Kaplan J, Lutsenko S. Functional properties of the copper-transporting ATPase ATP7B (the Wilson's disease protein) expressed in insect cells. J Biol Chem. 2002;277:976-83 pubmed
	Lunde P, Dahlstedt A, Bruton J, Lannergren J, Thoren P, Sejersted O, et al. Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure. Circ Res. 2001;88:1299-305 pubmed
	Takeda K, Inoue H, Tanizawa Y, Matsuzaki Y, Oba J, Watanabe Y, et al. WFS1 (Wolfram syndrome 1) gene product: predominant subcellular localization to endoplasmic reticulum in cultured cells and neuronal expression in rat brain. Hum Mol Genet. 2001;10:477-84 pubmed
	Antoine S, Pinet C, Coulombe A. Are B-type Ca2+ channels of cardiac myocytes akin to the passive ion channel in the plasma membrane Ca2+ pump?. J Membr Biol. 2001;179:37-50 pubmed
	Biser P, Thayne K, Kong J, Fleming W, Taylor D. Quantification of the alpha(3) subunit of the Na(+)/K(+)-ATPase in developing rat cerebellum. Brain Res Dev Brain Res. 2000;123:165-72 pubmed
	Johansson C, Lannergren J, Lunde P, Vennstrom B, Thoren P, Westerblad H. Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice. Am J Physiol Regul Integr Comp Physiol. 2000;278:R598-603 pubmed
	Arguello J, Peluffo R, Feng J, Lingrel J, Berlin J. Substitution of glutamic 779 with alanine in the Na,K-ATPase alpha subunit removes voltage dependence of ion transport. J Biol Chem. 1996;271:24610-6 pubmed
	Ganjeizadeh M, Zolotarjova N, Huang W, Askari A. Interactions of phosphorylation and dimerizing domains of the alpha-subunits of Na+/K(+)-ATPase. J Biol Chem. 1995;270:15707-10 pubmed
	Malik B, Jamieson G, Ball W. Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library. Protein Sci. 1993;2:2103-11 pubmed
	Ball W, Schwartz A, Lessard J. Isolation and characterization of monoclonal antibodies to (Na+ + K+)-ATPase. Biochim Biophys Acta. 1982;719:413-23 pubmed

product information

Product Type :

Antibody

Product Name :

ATP1A1 Monoclonal Antibody (M8-P1-A3)

Catalog # :

MA3-929

Quantity :

100µg

Price :

US 390

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Ovine, Porcine, Rat

Applications :

Flow Cytometry: 1/20 - 1/100, Immunocytochemistry: 1/50 - 1/200, Immunofluorescence: 1/50 - 1/200, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1/100 - 1/2000, Western Blot: 1:200-1:2000

Species :

Canine, Human, Mouse, Ovine, Porcine, Rat

Clone :

M8-P1-A3

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen :

Lamb kidney alpha 1 sodium/potassium ATPase.

Format :

Liquid

Applications w/Dilutions :

Aliases :

(Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; ATP1A1; atp1a1-a; atp1a1a.4; atp1a1-b; ATP1B; Atpa-1; ATPase Na+/K+ transporting subunit alpha 1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; BC010319; BOS_3049; K-ATPase; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na/K ATPase alpha 1 isoform; Na/K ATPase alpha 1 subunit; Na+/K+ ATPase; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ -ATPase alpha 1 subunit; Na+/K+ transporting alpha 1 polypeptide; Na+K+ ATPase; Na+K+ATPase; Nkaa1b; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium/potassium-ATPase alpha-subunit 1; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; unnamed protein product

more info or order :

Invitrogen product webpage