ATP1A1 Monoclonal Antibody (M7-PB-E9) | Invitrogen MA3-928 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ATP1A1 Monoclonal Antibody (M7-PB-E9)

catalog :

MA3-928

quantity :

400µL

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

M7-PB-E9

reactivity :

human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3c	Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, et al. Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours. Oncogene. 2017;36:4778-4789 pubmed publisher
western blot; human; 1:500; fig 1, 2	Madsen K, Hansen G, Danielsen E, Schousboe A. The subcellular localization of GABA transporters and its implication for seizure management. Neurochem Res. 2015;40:410-9 pubmed publisher
immunocytochemistry; human; 1:50; tbl 2	Gauss A, Buchholz I, Zahn A, Schmitz G, Stremmel W, Fuellekrug J, et al. Flotillin-2 expression in the human gut: from a cell model to human tissue in health and inflammatory bowel diseases. Int J Med Sci. 2013;10:1259-70 pubmed publisher
flow cytometry; domestic sheep	Kuzinski J, Zitnan R, Albrecht E, Viergutz T, Schweigel Röntgen M. Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet. Am J Physiol Regul Integr Comp Physiol. 2012;303:R909-20 pubmed publisher
western blot; human	Krapivinsky G, Krapivinsky L, Stotz S, Manasian Y, Clapham D. POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters. Proc Natl Acad Sci U S A. 2011;108:19234-9 pubmed publisher
western blot; human; 1:250	Li F, Ryu B, Krueger R, Heldt S, Albritton L. Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins. J Virol. 2012;86:373-81 pubmed publisher
immunohistochemistry; mouse	Nagata S, Jin Y, Yoshizato K, Kitamura M, Iizuka N, Song M, et al. Early uptake and continuous accumulation of thallium-201 chloride in a benign mixed tumor of soft tissue: case report. Diagn Pathol. 2010;5:34 pubmed publisher
immunocytochemistry; domestic sheep; 15:1000 western blot; domestic sheep; 1:1000	Kuzinski J, Zitnan R, Warnke Gurgel C, Schweigel M. The vacuolar-type H-ATPase in ovine rumen epithelium is regulated by metabolic signals. J Biomed Biotechnol. 2010;2010:525034 pubmed publisher
immunohistochemistry; rat	Tramoni M, Gilleron J, Tahiri K, Carette D, Corvol M, Segretain D, et al. Contraceptive steroids from pharmaceutical waste perturbate junctional communication in Sertoli cells. Biochimie. 2009;91:1366-75 pubmed publisher
immunocytochemistry; human	Jovov B, Orlando G, Tobey N, Brown K, Djukic Z, Carson J, et al. Ion transport and barrier function in a telomerase-immortalized human nondysplastic, Barrett's cell line (BAR-T). Dis Esophagus. 2009;22:386-95 pubmed
immunocytochemistry; bovine; 1:50 western blot; bovine	Graham C, Simmons N. Functional organization of the bovine rumen epithelium. Am J Physiol Regul Integr Comp Physiol. 2005;288:R173-81 pubmed
	Hansen G, Niels Christiansen L, Danielsen E. Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study. J Histochem Cytochem. 2008;56:677-85 pubmed publisher
	Dohan O, De La Vieja A, Carrasco N. Hydrocortisone and purinergic signaling stimulate sodium/iodide symporter (NIS)-mediated iodide transport in breast cancer cells. Mol Endocrinol. 2006;20:1121-37 pubmed
	Wang H, Luo W, Zhang Y, Li Y, Thinakaran G, Greengard P, et al. Presenilins and gamma-secretase inhibitors affect intracellular trafficking and cell surface localization of the gamma-secretase complex components. J Biol Chem. 2004;279:40560-6 pubmed
	Kizhatil K, Bennett V. Lateral membrane biogenesis in human bronchial epithelial cells requires 190-kDa ankyrin-G. J Biol Chem. 2004;279:16706-14 pubmed
	Qian Z, Donald R, Wang H, Chen Q, Albritton L. Identification of a critical basic residue on the ecotropic murine leukemia virus receptor. J Virol. 2003;77:8596-601 pubmed
	Wallace D, Christensen M, Reif G, Belibi F, Thrasher B, Herrell D, et al. Electrolyte and fluid secretion by cultured human inner medullary collecting duct cells. Am J Physiol Renal Physiol. 2002;283:F1337-50 pubmed
	Schuller S, Neefjes J, Ottenhoff T, Thole J, Young D. Coronin is involved in uptake of Mycobacterium bovis BCG in human macrophages but not in phagosome maintenance. Cell Microbiol. 2001;3:785-93 pubmed
	Bortner C, Gómez Angelats M, Cidlowski J. Plasma membrane depolarization without repolarization is an early molecular event in anti-Fas-induced apoptosis. J Biol Chem. 2001;276:4304-14 pubmed
	Low S, Miura M, Roche P, Valdez A, Mostov K, Weimbs T. Intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity. Mol Biol Cell. 2000;11:3045-60 pubmed
	Chen K, Lacayo N, Duran G, Cohen D, Sikic B. Loss of cyclosporin and azidopine binding are associated with altered ATPase activity by a mutant P-glycoprotein with deleted phe(335). Mol Pharmacol. 2000;57:769-77 pubmed
	Aloj L, Caraco C, Jagoda E, Eckelman W, Neumann R. Glut-1 and hexokinase expression: relationship with 2-fluoro-2-deoxy-D-glucose uptake in A431 and T47D cells in culture. Cancer Res. 1999;59:4709-14 pubmed
	Xu K, Huso D, Dawson T, Bredt D, Becker L. Nitric oxide synthase in cardiac sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 1999;96:657-62 pubmed
	Abbott A, Ball W. The epitope for the inhibitory antibody M7-PB-E9 contains Ser-646 and Asp-652 of the sheep Na+,K(+)-ATPase alpha-subunit. Biochemistry. 1993;32:3511-8 pubmed
	Malik B, Jamieson G, Ball W. Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library. Protein Sci. 1993;2:2103-11 pubmed
	Ball W, Schwartz A, Lessard J. Isolation and characterization of monoclonal antibodies to (Na+ + K+)-ATPase. Biochim Biophys Acta. 1982;719:413-23 pubmed
	Abbott A, Ball W. The inhibitory monoclonal antibody M7-PB-E9 stabilizes E2 conformational states of Na+,K(+)-ATPase. Biochemistry. 1992;31:11236-43 pubmed

image

image 1 :

Immunofluorescent analysis of Sodium/Potassium ATPase alpha using Sodium/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product# MA3-928 ) shows staining in Hela Cells. Sodium/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodium/Potassium ATPase alpha (Product# MA3-928 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

ATP1A1 Monoclonal Antibody (M7-PB-E9)

Catalog # :

MA3-928

Quantity :

400µL

Price :

US 390

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Canine, Chicken, Human, Mouse, Ovine, Porcine, Rat

Applications :

ELISA: Assay dependent, Immunocytochemistry: Assay dependent, Immunofluorescence: 1:20, Immunohistochemistry: 1:100, Immunoprecipitation: Assay dependent, Western Blot: 1:500-1:5000

Species :

Bovine, Canine, Chicken, Human, Mouse, Ovine, Porcine, Rat

Clone :

M7-PB-E9

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen :

Purified alpha sodium/potassium ATPase from sheep kidney.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay dependent, Immunocytochemistry: Assay dependent, Immunofluorescence: 1:20, Immunohistochemistry: 1:100, Immunoprecipitation: Assay dependent, Western Blot: 1:500-1:5000

Aliases :

(Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; ATP1A1; atp1a1-a; atp1a1a.4; atp1a1-b; ATP1B; Atpa-1; ATPase Na+/K+ transporting subunit alpha 1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; BC010319; BOS_3049; K-ATPase; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na/K ATPase alpha 1 isoform; Na/K ATPase alpha 1 subunit; Na+/K+ ATPase; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ -ATPase alpha 1 subunit; Na+/K+ transporting alpha 1 polypeptide; Na+K+ ATPase; Na+K+ATPase; Nkaa1b; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium/potassium-ATPase alpha-subunit 1; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; unnamed protein product

more info or order :

Invitrogen product webpage